scholarly journals Diffusible and Volatile Antifungal Compounds Produced by Pseudomonas chlororaphis subsp. aurantiaca ST-TJ4 against Various Phytopathogenic Fungi

2020 ◽  
Author(s):  
Wei-Liang Kong ◽  
Pu-Sheng Li ◽  
Xiao-Qin Wu ◽  
Tian-Yu Wu ◽  
Xiao-Rui Sun
Keyword(s):  
Control Plant ◽  
Pathogenic Fungi ◽  
Plant Growth Promoting Rhizobacteria ◽  
Phytopathogenic Fungi ◽  
Plant Diseases ◽  
Pseudomonas Chlororaphis ◽  
Headspace Collection ◽  
Rdna Sequences ◽  
16S Rdna Sequences ◽  
Plant Growth Promoting

Abstract Plant growth-promoting rhizobacteria can potentially be used as an alternative strategy to control plant disease. In this study, strain ST-TJ4 isolated from the rhizosphere soil of a healthy poplar was found to have strong antifungal activity against 11 phytopathogenic fungi in agriculture and forestry. Strain ST-TJ4 was identified as Pseudomonas chlororaphis subsp. aurantiaca based on 16S rDNA sequences. The bacterium can produce siderophores, cellulase, and protease, and has genes involved in the synthesis of phenazine, 1-phenazinecarboxylic acid, pyrrolnitrin, and hydrogen cyanide. Moreover, the volatile compounds released by strain ST-TJ4 can inhibit the mycelial growth of plant pathogenic fungi more than diffusible substances can. Based on volatile compound profiles of strain ST-TJ4 obtained from headspace collection and GC-MS/MS analysis, 1-undecene was identified. In summary, the results suggested that P. chlororaphis subsp. aurantiaca ST-TJ4 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.

scholarly journals Forest Tree Associated Bacterial Diffusible and Volatile Organic Compounds against Various Phytopathogenic Fungi

2020 ◽  
Vol 8 (4) ◽  
pp. 590 ◽  
Author(s):  
Wei-Liang Kong ◽  
Pu-Sheng Li ◽  
Xiao-Qin Wu ◽  
Tian-Yu Wu ◽  
Xiao-Rui Sun
Keyword(s):  
Control Plant ◽  
Pathogenic Fungi ◽  
Plant Growth Promoting Rhizobacteria ◽  
Forest Tree ◽  
Phytopathogenic Fungi ◽  
Plant Diseases ◽  
Pseudomonas Sp ◽  
Headspace Collection ◽  
Plant Growth Promoting ◽  
Encoding Gene

Plant growth-promoting rhizobacteria (PGPR) can potentially be used as an alternative strategy to control plant diseases. In this study, strain ST–TJ4 isolated from the rhizosphere soil of a healthy poplar was found to have a strong antifungal activity against 11 phytopathogenic fungi in agriculture and forestry. Strain ST–TJ4 was identified as Pseudomonas sp. based on 16S rRNA-encoding gene sequences. The bacterium can produce siderophores, cellulase, and protease, and has genes involved in the synthesis of phenazine, 1–phenazinecarboxylic acid, pyrrolnitrin, and hydrogen cyanide. Additionally, the volatile compounds released by strain ST–TJ4 can inhibit the mycelial growth of plant pathogenic fungi more than diffusible substances can. Based on volatile compound profiles of strain ST–TJ4 obtained from headspace collection and GC–MS/MS analysis, 1-undecene was identified. In summary, the results suggested that Pseudomonas sp. ST–TJ4 can be used as a biocontrol agent for various plant diseases caused by phytopathogenic fungi.

scholarly journals Characterization of Bacteria Isolated from the Saffron (Crocus sativus L.) Rhizosphere

2017 ◽  
Vol 25 (2) ◽  
pp. 5-14
Author(s):  
Majid Jami Al-Ahmadi ◽  
Abbas Mohammadi ◽  
Esmaeil Salehi Kohabadi
Keyword(s):  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Crocus Sativus ◽  
Phenotypic Traits ◽  
Rdna Sequences ◽  
16S Rdna Sequences ◽  
Plant Growth Promoting ◽  
Sequences Analysis ◽  
Crocus Sativus L

Abstract One purpose of assessing the soil alive and active community is the identification of beneficial bacteria to use them as biological fertilizers, replacing or supplementing synthetic fertilizers. Such biofertilizers are predicted for the sustainability of agricultural production, especially for low input systems such as saffron fields. The aim of this work was to isolate and identify saffron rhizobacteria and to evaluate their possible effects on saffron growth. During 2013/14, some bacteria were isolated from the rhizosphere of the saffron plantations of different age in Gol village, Birjand, Iran. In total, 12 bacteria species were identified based on phenotypic traits and 16S rDNA sequences analysis. The strains were identified as B. subtilis, B. anthracis, B. cereus, B. megaterium, Bacillus sp., Paenibacillus, Pseudomonas fluorescens, P. putida, Escherichia coli, Pectobacterium sp. and Pantoea sp., with the dominant population belonging to the genus Bacillus. In the field study, inoculation of soil with these strains did not affect the leaf dry weight of the cultivated saffron, however, the strains of P. fluorescens increased the leaf area while P. fluorescens, Paenibacillus, Pectobacterium and B. megaterium increased the number of daughter corms and Azotobacter, B. cereus, B. subtilis and B. megaterium increased the corm weight. Our finding revealed that some bacteria present in the soil of perennial saffron plantations have a promising potential for developing as a plant growth promoting rhizobacteria.

scholarly journals Characterization of potential plant growth-promoting rhizobacteria isolated from sunflower (Helianthus annuus L.) in Iran

2019 ◽  
Vol 70 (4) ◽  
pp. 268-277
Author(s):  
Nabi Khezrinejad ◽  
Gholam Khodakaramian ◽  
Fatemeh Shahryari
Keyword(s):  
Genetic Diversity ◽  
Plant Growth Promoting Rhizobacteria ◽  
Pgp Traits ◽  
North West ◽  
Rdna Sequences ◽  
16S Rdna Sequences ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Identification And Characterization

Purpose This study aims to characterize plant growth-promoting rhizobacteria (PGPR) in sunflowers growing in different locations at North West of Iran. Materials and methods Sunflower plants were collected from different regions of West Azarbaijan, and rhizospheric bacterial strains were isolated and screened for PGP traits. Identification and characterization of the PGPR were conducted based on 16s rDNA sequences and phenotypic analysis, the strains clustered for genetic diversity by rep-PCR method. Results Among the 80 bacterial isolates, 20 showed PGP traits and were selected for other potentials. All the selected isolates produced indole-3-acetic acid at the rate of 9.2–33.7 mg/ml. In addition, 13, 15, 12, and 16 were positive for phosphate solubilization, siderephore, hydrogen cyanide, and ammonia production, respectively. The results from a subsequent pot experiment indicated that PGPRs distinctly increased sun flower shoot and root length, shoot and root fresh weight, as well as shoot and root dry weight. Based on 16S rDNA sequences and biochemical and physiological characteristics, 20 PGPRs were identified as Pseudomonas fluorescens (five isolates), Pseudomonas aeruginosa (four isolates), Pseudomonas geniculata (one isolate), Bacillus subtilis (four isolates), Bacillus pumilus (two isolates), Stenotrophomonas maltophilia (two isolates), and Brevibacterium frigoritolerans (two isolates). In rep-PCR, PGPR isolates were differentiated into seven clusters (A–G) at 65% similarity level. These results demonstrated the existence of a considerable species richness and genetic diversity among PGPRs isolated from different regions of North West of Iran. Conclusions To the best of our knowledge, this is first report for the identification and characterization of B. frigoritolerans as PGPR in sunflower plants.

scholarly journals The fabrication of the complex bio-fertilizer for wheat cultivation based on collection bacteria of the PGPR group

2020 ◽  
Vol 21 (11) ◽  
Author(s):  
RAKHILYA AIPOVA ◽  
AIZHAN ABDYKADYROVA ◽  
DMITRY SILAYEV ◽  
ERKIN TAZABEKOVA ◽  
IRINA OSHERGINA ◽  
...  
Keyword(s):  
Wheat Yield ◽  
Practical Interest ◽  
Plant Growth Promoting Rhizobacteria ◽  
Phytopathogenic Fungi ◽  
Plant Diseases ◽  
Bacterial Strains ◽  
Biological Products ◽  
Wheat Cultivation ◽  
Plant Growth Promoting ◽  
Wheat Products

Abstract. Aipova R, Abdykadyrova A, Silayev D, Tazabekova E, Oshergina I, Ten E, Kurmanbaye A. 2020. The fabrication of the complex bio-fertilizer for wheat cultivation based on collection bacteria of the PGPR group. Biodiversitas 21: 5021-5028. The development of new types of biological products based on microbial complexes from local bacterial strains is a great theoretical and practical interest for agriculture. It can provide an opportunity for better preservation of the natural properties of the wheat products under extreme conditions. The aim of this study was to obtain and test a biological product to increase wheat productivity in northern Kazakhstan. Our data indicate the potential of Plant Growth-Promoting Rhizobacteria (PGPR) group bacteria for the development of biofertilizers and biopesticides. For instance, the bacteria B. mojavensis showed effectiveness in the experiments with the wheat (Astana-2 type). We observed an increase in wheat yield by 15% under conditions of artificial infection of crops with snow mold (by 2.5fold compared with the control). The results demonstrated that the B. mojavensis Lhv 97 strain can be used as an ingredient of biological products due to its activity against plant diseases caused by phytopathogenic fungi.

The use of Bacillus spp. as bacterial biocontrol agents to control plant diseases

2021 ◽  
Author(s):  
Adrien Anckaert ◽  
◽  
Anthony Arguelles Arias ◽  
Grégory Hoff ◽  
Maryline Calonne-Salmon ◽  
...  
Keyword(s):  
Control Plant ◽  
Ecological Factors ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Diseases ◽  
Biocontrol Agents ◽  
Systemic Resistance ◽  
Health Improvement ◽  
Wide Range ◽  
Bacillus Spp ◽  
Plant Growth Promoting

Biocontrol agents (BCAs) based on plant growth promoting rhizobacteria have recently been developed as alternatives to chemical pesticides. Among those beneficial bacteria, Bacillus spp. are one of the most promising BCAs. A wide range of bioactive secondary metabolites (BSMs) are involved in biocontrol via antibiosis to phytopathogens and/or via elicitation of systemic resistance in their host plants. This chapter illustrates the diversity of pathosystems in which BCA based on Bacillus spp. have proved effective. It describes the mechanisms underpinning this biocontrol activity via production of a wide range of enzymes, proteins and small-size BSMs. As these BSMs are clearly involved in pathogen control, we emphasise the importance of understanding the ecological factors influencing their production. In the last part of the chapter, we highlight the potential interactions between Bacillus spp. and other soil microorganisms in developing consortia of biocontrol agents combining species with synergistic activities for plant health improvement.

scholarly journals NITROGEN FIXING BACTERIA AZOTOBACTER AS BIOFERTILIZER AND BIOCONTROL IN LONG BEAN

Agric ◽  
2018 ◽  
Vol 30 (1) ◽  
pp. 25-32
Author(s):  
Reginawanti Hindersah ◽  
Marthin Kalay ◽  
Abraham Talahaturuson ◽  
Yansen Lakburlawal
Keyword(s):  
Block Design ◽  
Control Plant ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Diseases ◽  
Damping Off ◽  
Randomized Block Design ◽  
Plant Growth Promoting ◽  
Npk Fertilizer ◽  
Growth Promoting ◽  
And Control

Azotobacter is Plant Growth Promoting Rhizobacteria through the mechanism of nitrogen fixation and phytohormon production but this rhizobacteria has a role to control plant diseases. The objective of experiment was to evaluate the activity of Azotobacter as biofertilizers as well as biocontrol on long bean cultivation in damping off endemic land in Ambon city, Maluku Province. The field experiment was arranged in completely randomized block design. Inoculation of long bean by Azotobacter has been done by seed inoculation, soil inoculation before planting, and plant inoculation. Plants treated with Azotobacter received fertilizer NPK of ¾ or ½ dosage recommendation while control plants were received 100% NPK. Research showed that no differences between yield of long bean inoculated with Azotobacter sp +reduced doses of NPK with that of control plants. Any application method of Azotobacter inoculation lowered damping off diseases incidence significantly until 10 days after planting, but no effect of inoculation on late blight at 21 days after planting. This study confirmed that Azotobacter has dual activity to reduce the dose of NPK fertilizer and control damping off.

scholarly journals Biocontrol potential; antifungal activity and plant growth promoting activities of endophytic bacteria from Raphanus sativus

2021 ◽  
Vol 12 (2) ◽  
pp. 1143-1150
Author(s):  
Lavanya J ◽  
Chanthosh S ◽  
Reshma Shrii ◽  
Viknesh V ◽  
Deepika S ◽  
...  
Keyword(s):  
16S Rrna ◽  
Plant Growth ◽  
Raphanus Sativus ◽  
Endophytic Bacteria ◽  
Effective Strain ◽  
Pathogenic Fungi ◽  
Plant Diseases ◽  
Plant Pathogenic Fungi ◽  
Plant Growth Promoting ◽  
Growth Promoting

The study was aimed to find an alternate approach for chemicals used in agriculture to avoid microbial infections. Fungal pathogens cause different types of plant diseases and affect a majority of edible crops by destroying the tissues of the plant in a direct or indirect mechanism. So, an alternative approach led to the development of biocontrol agents using endophytic  bacteria. A total of 8 endophytic bacteria were isolated from the root, stem, and leaves of radish (Raphanus sativus). The antagonistic activity of these bacteria against the 2 isolated plant pathogenic fungi was determined in vitro. Two out of eight bacteria showed more than 50% inhibitory activity against one fungus, were further characterized using the 16s rRNA sequencing method. On the basis of the phylogenetic tree of the 16s rRNA method, the endophytic bacterial samples were identified as Tonsilliphilus suis  and Exiguobacterium aurantiacum against plant pathogenic Aspergillus flavus  isolated from Raphanus sativus, which makes them highly suitable as an alternative for chemical fertilizers to provide resistance to plant pathogenic fungi. The cell wall degrading activities such as protease activity, amylase activity, and plant growth-promoting properties such as Hydrogen cyanide (HCN), Indole acetic acid (IAA), ammonia production of these endophytic bacteria were evaluated. The results show that T. suis  is the most effective strain for radish growth development.

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