Microbial Identification using V1-V4 Hypervariable Region of 16S ribosomal RNA gene

2021 ◽  
Vol 15 (2) ◽  
pp. 142-152
Author(s):  
Hyun-jung An ◽  
Miwha Chang ◽  
Minji Lee ◽  
Haeyong Lee ◽  
Seohyun Moon
Keyword(s):  
Ribosomal Rna ◽  
Hypervariable Region ◽  
16S Ribosomal Rna ◽  
Ribosomal Rna Gene ◽  
Microbial Identification ◽  
16S Ribosomal Rna Gene

scholarly journals Molecular Approaches to Studying Microbial Communities: Targeting the 16S Ribosomal RNA Gene

2016 ◽  
Vol 38 (3) ◽  
pp. 223-232 ◽  
Author(s):  
Kazumasa FUKUDA ◽  
Midori OGAWA ◽  
Hatsumi TANIGUCHI ◽  
Mitsumasa SAITO
Keyword(s):  
Microbial Communities ◽  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Approaches To Studying ◽  
Ribosomal Rna Gene ◽  
Molecular Approaches ◽  
16S Ribosomal Rna Gene

scholarly journals Evaluation of 16S, map1 and pCS20 probes for detection of Cowdria and Ehrlichia species

1999 ◽  
Vol 122 (2) ◽  
pp. 323-328 ◽  
Author(s):  
M. T. E. P. ALLSOPP ◽  
C. M. HATTINGH ◽  
S. W. VOGEL ◽  
B. A. ALLSOPP
Keyword(s):  
16S Rrna ◽  
Ribosomal Rna ◽  
Pcr Amplification ◽  
16S Ribosomal Rna ◽  
16S Rrna Genes ◽  
Rrna Genes ◽  
Amblyomma Hebraeum ◽  
Ribosomal Rna Gene ◽  
Group Iii ◽  
16S Ribosomal Rna Gene

A panel of 16S ribosomal RNA gene probes has been developed for the study of the epidemiology of heartwater; five of these detect different cowdria genotypes, one detects five distinct genotypes; one detects any Group III Ehrlichia species other than Cowdria and one detects any Group II Ehrlichia species. These probes have been used on PCR-amplified rickettsial 16S rRNA genes from over 200 Amblyomma hebraeum ticks. Control ticks were laboratory-reared and either uninfected or fed on sheep experimentally infected with different cowdria isolates, field ticks were collected from animals in heartwater-endemic areas. All tick-derived DNA samples were also examined by PCR amplification and probing for two other cowdria genes (map1 and pCS20) which have previously been used for heartwater epidemiology. This paper describes the first direct comparison of all currently available DNA probes for heartwater-associated organisms.

scholarly journals A Case of Sepsis in a 92-Year-Old Korean Woman Caused byAerococcus urinaeand Identified by Sequencing the 16S Ribosomal RNA Gene

2016 ◽  
Vol 47 (2) ◽  
pp. e15-e17 ◽  
Author(s):  
Min Young Lee ◽  
Myeong Hee Kim ◽  
Woo In Lee ◽  
So Young Kang ◽  
You La Jeon
Keyword(s):  
Ribosomal Rna ◽  
16S Ribosomal Rna ◽  
Korean Woman ◽  
Ribosomal Rna Gene ◽  
16S Ribosomal Rna Gene

scholarly journals Bacterial diversity in aortic aneurysms determined by 16S ribosomal RNA gene analysis

2006 ◽  
Vol 44 (5) ◽  
pp. 1055-1060 ◽  
Author(s):  
Rafael Marques da Silva ◽  
Dominique A. Caugant ◽  
Emenike R.K. Eribe ◽  
Jørn A. Aas ◽  
Per S. Lingaas ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
Ribosomal Rna ◽  
Aortic Aneurysms ◽  
16S Ribosomal Rna ◽  
Gene Analysis ◽  
Ribosomal Rna Gene ◽  
16S Ribosomal Rna Gene
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