The role of human papillomavirus deoxyribonucleic acid for distinguishing  between cervical adenocarcinoma and endometrial adenocarcinoma

HIGHLIGHTS1. Determining primary tumor between uterine cervical adenocarcinoma and endometrial carcinoma is often complicated due to the lack of specimen or overlapped histological and morphological spectrum.2. The distinction between endometrial and endocervical adenocarcinoma preoperatively is important because the treatment is different.3. The samples from each group are tested for HPV DNA using PCR method.4. High risk HPV infected patients have a higher probability to become uterine cervical adenocarcinoma.5. HPV DNA test has a role for distinguishing between uterine cervical adenocarcinoma and endometrial carcinoma. ABSTRACTObjectives: To analyze the role of HPV DNA for distinguishing between uterine cervical adenocarcinoma and endometrial adenocarcinoma.Materials and Methods: This was a case control study using paraffin block samples from uterine cervix adenocarcinoma and endometrial adenocarcinoma operation at Dr. Soetomo General Academic Hospital, Surabaya, Indonesia. Each group was tested for HPV DNA using PCR method. Sample size was 18 in each group.Results: A total of 36 samples fulfilled the inclusion criteria in this study. Each group comprised 18 samples. There were 83.3% of uterine cervical adenocarcinoma and 11.1% of endometrial adenocarcinoma that revealed high risk HPV. Chi-Square test result found significant correlation between high risk HPV and uterine cervical adenocarcinoma (p<0.05) with Odds Ratio (OR) 40.00 (CI 95%).Conclusion: There was a significant correlation between high risk HPV and uterine cervix adenocarcinoma. High-risk HPV infected patients had a risk to suffer from uterine cervical adenocarcinoma compared to those with endometrial adenocarcinoma. HPV DNA test had a role for distinguishing between uterine cervical adenocarcinoma and endometrial adenocarcinoma.

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Clinical Laboratory Experience with Cervista HPV HR as a Function of Cytological Classification: Comparison with RetrospectivedigeneHC2 High-Risk HPV DNA Test Data

Journal of Clinical Microbiology ◽

10.1128/jcm.02836-12 ◽

2012 ◽

Vol 51 (3) ◽

pp. 1057-1058 ◽

Cited By ~ 6

Author(s):

Brian K. Du Chateau ◽

Elizabeth R. Schroeder ◽

Erik Munson

Keyword(s):

High Risk ◽

Test Data ◽

Clinical Laboratory ◽

Laboratory Experience ◽

Dna Test ◽

Hpv Dna ◽

High Risk Hpv ◽

Hpv Dna Test

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The Evaluation of p16ink4a Immunoexpression/Immunostaining and Human Papillomavirus DNA Test in Cervical Liquid-Based Cytological Samples

International Journal of Gynecological Cancer ◽

10.1097/igc.0b013e3182009eea ◽

2011 ◽

Vol 21 (1) ◽

pp. 79-85 ◽

Cited By ~ 21

Author(s):

Maria Nasioutziki ◽

Angelos Daniilidis ◽

Kostos Dinas ◽

Maria Kyrgiou ◽

George Valasoulis ◽

...

Keyword(s):

High Risk ◽

Hpv Infection ◽

Low Grade ◽

Squamous Intraepithelial Lesion ◽

Dna Test ◽

Hpv Dna ◽

Squamous Cells ◽

Intraepithelial Lesion ◽

High Risk Hpv ◽

Hpv Dna Test

Aim:To evaluate the role of p16INK4a immunoexpression and human papillomavirus (HPV) DNA test for the detection of dyskaryotic cells in high-risk women.Materials and Methods:This work was a retrospective diagnostic study conducted in the University Hospital of Thessaloniki from January to December 2008. The subjects were women with current or previous HPV infection and current or previous cervical intraepithelial lesion (with or without treatment) or clinical warts. All liquid-based cytological samples were tested for P16INKa and HPV DNA test. The accuracy parameters used for the outcome included sensitivity, specificity, and positive predictive value.Results:A total of 226 women were included; the mean age was 29 years. Expression of p16INK4a was detected in the cytological samples of 13% of the negative cases, 44% of the cases of atypical squamous cells of undetermined significance, 46% of the cases of low-grade squamous intraepithelial lesion, and 78% of the cases of high-grade squamous intraepithelial lesion. A total of 91 women tested positive for high-risk HPV infection, and 54 of those had p16INK4a-positive staining reaction cells. The concordance between the 2 tests, HPV DNA and p16, was 59% regarding infection-positive cases. Diffuse strong parabasal p16INK4a immunostaining (nuclear score >2) was observed in 17 cases of the abnormal cytological findings (atypical squamous cells of undetermined significance, 2 cases; low-grade squamous intraepithelial lesion, 8 cases; high-grade squamous intraepithelial lesion, 7 cases). Colposcopy-directed biopsies were used as the criterion standard for the detection of cervical intraepithelial neoplasia in 91 women. The sensitivity of p16INK4a was 95% and the specificity was 92%, whereas the sensitivity of high-risk HPV was 100% and the specificity was 78%. The positive predictive value of p16INK4a was 71%, whereas that of HPV DNA was 44%.Conclusion:The findings suggest that p16INK4a immunostaining can improve the accuracy of cytological examination and HPV DNA test and may be particularly useful in the triage of low-grade lesions.

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