scholarly journals Identification of antibiotic resistance patterns in Helicobacter pylori strains isolated from gastric biopsies using real-time PCR and genotypic analysis

2021 ◽  
Author(s):  
Juanita Spiteri
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Real Time ◽  
Real Time Pcr ◽  
Genotypic Analysis ◽  
Resistance Patterns ◽  
Gastric Biopsies ◽  
Antibiotic Resistance Patterns

scholarly journals 1445Antibiotic susceptibility of Helicobacter pylori in Arctic Canadian Indigenous communities

2021 ◽  
Vol 50 (Supplement_1) ◽  
Author(s):  
Lauren Lindsey ◽  
Doug Quilty ◽  
Taylor Cromarty ◽  
Ali Assi ◽  
Sander Veldhuyzen van Zanten ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Disease Burden ◽  
Gastrointestinal Endoscopy ◽  
Indigenous Communities ◽  
Upper Gastrointestinal Endoscopy ◽  
Antibiotic Resistant ◽  
Resistance Patterns ◽  
Gastric Biopsies ◽  
Antibiotic Resistance Patterns ◽  
Upper Gastrointestinal

Abstract Background Our community-driven projects address concerns of Canadian Arctic Indigenous communities about Helicobacter pylori (Hp) infection, responsible for elevated gastric cancer mortality in the region. A key concern is poor effectiveness of anti-Hp treatment. We aimed to describe antibiotic resistance patterns in Hp isolated from project participants. Methods Participants in 7 communities underwent upper gastrointestinal endoscopy with 2 gastric biopsies taken for tissue culture during 2008-2017. We tested Hp isolates for resistance to 7 antibiotics by Etest and assessed 4 outcomes: resistance to metronidazole, clarithromycin, 1+ antibiotics, and 2+ antibiotics. We tabulated proportion positive among isolates tested with 95% confidence intervals (CI) and used logistic regression to assess the relation of age and sex to resistance outcomes. Results Of 259 Hp isolates tested, resistance to metronidazole, clarithromycin, 1+ antibiotics, and 2+ antibiotics were (% [CI]): respectively, 35 [29-41], 19 [15-25], 44 [38-50], and 12 [8-17] overall; 38 [30-47], 24 [17-32], 49 [41-58], and 16 [11-23] in 146 isolates from women; and 30 [22-39], 13 [8-21], 36 [27-46], and 6 [3-12] in 113 isolates from men. Odds of resistance to clarithromycin, 1+ antibiotics, 2+ antibiotics, and, to a lesser degree metronidazole, were elevated in women relative to men after age adjustment and increased with age in women but not men. Conclusion In Arctic Indigenous communities in Canada, women were more likely than men to harbor antibiotic-resistant Hp, and their frequency of resistant Hp infection increased with age. Key messages Community-driven research answers questions posed by those who bear the disease burden.

Change of point mutations in Helicobacter pylori rRNA associated with clarithromycin resistance in Italy

2014 ◽  
Vol 63 (3) ◽  
pp. 453-457 ◽  
Author(s):  
Vincenzo De Francesco ◽  
Angelo Zullo ◽  
Floriana Giorgio ◽  
Ilaria Saracino ◽  
Cristina Zaccaro ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Real Time ◽  
Real Time Pcr ◽  
Single Point ◽  
Point Mutations ◽  
Clarithromycin Resistance ◽  
Genotypic Analysis ◽  
Previously Treated ◽  
Resistant Strains ◽  
H Pylori

Primary clarithromycin resistance is the main factor affecting the efficacy of Helicobacter pylori therapy. This study aimed: (i) to assess the concordance between phenotypic (culture) and genotypic (real-time PCR) tests in resistant strains; (ii) to search, in the case of disagreement between the methods, for point mutations other than those reported as the most frequent in Europe; and (iii) to compare the MICs associated with the single point mutations. In order to perform real-time PCR, we retrieved biopsies from patients in whom H. pylori infection was successful diagnosed by bacterial culture and clarithromycin resistance was assessed using the Etest. Only patients who had never been previously treated, and with H. pylori strains that were either resistant exclusively to clarithromycin or without any resistance, were included. Biopsies from 82 infected patients were analysed, including 42 strains that were clarithromycin resistant and 40 that were clarithromycin susceptible on culture. On genotypic analysis, at least one of the three most frequently reported point mutations (A2142C, A2142G and A2143G) was detected in only 23 cases (54.8 %), with a concordance between the two methods of 0.67. Novel point mutations (A2115G, G2141A and A2144T) were detected in a further 14 out of 19 discordant cases, increasing the resistance detection rate of PCR to 88 % (P<0.001; odds ratio 6.1, 95 % confidence interval 2−18.6) and the concordance to 0.81. No significant differences in MIC values among different point mutations were observed. This study suggests that: (i) the prevalence of the usually reported point mutations may be decreasing, with a concomitant emergence of new mutations; (ii) PCR-based methods should search for at least six point mutations to achieve good accuracy in detecting clarithromycin resistance; and (iii) none of the tested point mutations is associated with significantly higher MIC values than the others.

Helicobacter pylori Antibiotic resistance patterns among Egyptian children and predictors of resistance

QJM ◽  
2020 ◽  
Vol 113 (Supplement_1) ◽  
Author(s):  
Y M M M Awad ◽  
M T Eldeeb ◽  
M S Fathi ◽  
N Mahmoud ◽  
R M E Morsy
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Pediatric Patients ◽  
Gi Symptoms ◽  
Egyptian Children ◽  
Urease Test ◽  
Resistance Patterns ◽  
Culture And Sensitivity ◽  
Antibiotic Resistance Patterns ◽  
Resistance Data

Abstract Background Most individuals are infected by Helicobacter pylori during early childhood; in developing countries, 50% of children are infected by the age of 5 years. Successful eradication is important to prevent the development of antibiotic resistance, as well as to reduce the number of treatments and procedures. Among children receiving the standard triple therapy regimen, eradication rates are declining. Thus, national/ regional antibiotic resistance data could be used to guide treatment regimens for H pylori infection Aim of the work to evaluate the antibiotic resistance patterns of Helicobacter pylori strains among pediatric patients and the predictors of this resistance. Patients and Methods Cross sectional, carried out on 30 pediatric patients who presented to gastroenterology unit Ain Shams University for endoscopic evaluation of their upper GI symptoms including hematemesis, vomiting and abdominal pain. Five gastric biopsies were taken, two from the corpus for histopathology and culture, and 3 from the antrum, where one of them was sent for histopathology, and the second one for rapid urease test, and the third one was sent for culture and sensitivity. Results Results of culture and sensitivity has shown that resistance to amoxicillin was 20%, to clarithromycin was 50%, metronidazole resistance was 86.7%, tetracycline resistance showed to be 13.3%, finally levofloxacin resistance was 6.7%. Excess intake of fat was associated with resistance to amoxicillin and clarithromycin. While, duodenal ulcer and duodenal nodularity were associated with resistance to tetracycline and levofloxacin respectively. Conclusion The eradication regimen in Egypt should be revised according to our national antibiotic resistance data. Further studies are needed to assess predictors of resistance to antibiotics.

scholarly journals Prevalence and Antibiotic Resistance Patterns of Helicobacter pylori Infection in Koh Kong, Cambodia

2020 ◽  
Vol 21 (5) ◽  
pp. 1409-1413 ◽  
Author(s):  
Natsuda Aumpan ◽  
Ratha-Korn Vilaichone ◽  
Pornpen Gamnarai ◽  
Likhasit Sanglutong ◽  
Thawee Ratanachu-ek ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Antibiotic Resistance ◽  
Helicobacter Pylori Infection ◽  
Resistance Patterns ◽  
Antibiotic Resistance Patterns
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