Management of Wilt and Root Rot of Chickpea caused by Fusarium oxysporum f. sp. ciceri and Macrophomina phaseolina through Seed Biopriming and Soil Application of Bio-Agents

BurkholderiaSP. Strain TNAU-1 for Biological Control of Root Rot in Mung Bean (Vigna RadiataL.) Caused byMacrophomina PhaseolinaThe potential ofBurkholderiasp. strain TNAU-1 for the management of mung bean (Vigna radiataL.) root rot caused byMacrophomina phaseolinawas evaluated under greenhouse conditions.Burkholderiasp. strain TNAU-1 inhibited the mycelial growth ofM. phaseolina in vitroand produced an inhibition zone of 18.8 mm. Mung bean seeds when treated with the bacterial suspension, showed significant increase in root length, shoot length and seedling vigour. A talc-based powder formulation ofBurkholderiasp. strain TNAU-1 was developed and evaluated for its efficacy in the management of mung bean root rot under greenhouse conditions. Seed treatment or soil application of the powder formulation ofBurkholderiasp. strain TNAU-1 significantly reduced the incidence of root rot and increased the germination percentage and plant height. Seed treatment with the powder formulation ofBurkholderiasp. strain TNAU-1 alone was effective in controlling root rot disease; but the combined seed treatment and soil application ofBurkholderiasp. strain TNAU-1, increased the efficacy. Seed treatment and soil application withBurkholderiasp. reduced the root rot incidence from 52.6 per cent (with non-bacterized seeds) to 16.7 per cent. Control of root rot with the application ofBurkholderiasp. by seed treatment and soil application was not statistically different from that obtained with seed treatment with carbendazim. The endophytic movement ofBurkholderiasp. in the stem, roots and leaves of mung bean was confirmed through PCR usingBurkholderiasp. specific primers which resulted in the amplification of a 417 bp product.

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Management of root-rot disease complex of mungbean caused by Macrophomina phaseolina and Rhizoctonia solani through soil application of Trichoderma spp.

Crop Protection ◽

10.1016/j.cropro.2019.01.014 ◽

2019 ◽

Vol 119 ◽

pp. 24-29 ◽

Cited By ~ 4

Author(s):

Mujeebur Rahman Khan ◽

Ziaul Haque ◽

Farahnaz Rasool ◽

Khalid Salati ◽

Uzma Khan ◽

...

Keyword(s):

Rhizoctonia Solani ◽

Root Rot ◽

Macrophomina Phaseolina ◽

Trichoderma Spp ◽

Soil Application ◽

Disease Complex ◽

Root Rot Disease ◽

Rot Disease

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Halo Tolerance of Biocontrol Agents against Root Rot of Mung Bean (Vigna radiata (L.) Wilczek var. radiata) Caused by Macrophomina phaseolina (Tassi) Goid in Salt Affected Soils

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2021/v33i2430784 ◽

2021 ◽

pp. 316-326

Author(s):

P. T. Sharavanan ◽

V. K. Satya ◽

M. Rajesh

Keyword(s):

Field Condition ◽

Root Rot ◽

Trichoderma Viride ◽

Soil Condition ◽

Macrophomina Phaseolina ◽

Biocontrol Agents ◽

Soil Application ◽

Salt Affected Soils ◽

Ph Level

Root rot of mung bean [Vigna radiata (L.) Wilczek var. radiata] is major disease and claims huge yield loss if they occur in the field. The pathogen is basically soil borne and survivability may vary depends on soil condition. The fungicide chemicals are available to manage the disease; however, the biocontrol agents are nowadays available for the disease management and the microbial activity of the biocontrol agents is influenced by existing soil condition including soil pH. Hence, a study was conducted to find out the halo tolerance capacity of the biocontrol agents against root rot disease in salt affected soils under in vitro, in vivo and field condition. The root rot pathogen Macrophomina phaseolina was isolated from infected root. Efficacy of biocontrol agents against growth of M. phaseolina was assessed in vitro. The results revealed that TNAU strain of Bacillus subtilis reduced the mycelial growth of the M. phaseolina significantly when media supplemented with NaCl at 5% (1.4 cm), 7.5% (1.5 cm), 10% (1.6cm) and 12.5% (1.6 cm) and without NaCl (1.2 cm) and similar trend of reduction also expressed by BCA1 strain of B. subtilis, Pseudomonas fluorescens and Trichoderma viride under in vitro. The performance of the biocontrol agents against the pathogen is slightly reduced when media supplemented with NaCl. The reduction of mycelia weight of M.phaeolina was more in media added with TNAU strain of B.subtilis and the performance of TNAU strain of B.subtilis on reduction of mycelial weight of M.phaseolina is reduced when the broth added with NaCl at 5% (3.15g), 7.5% (3.25g), 10% (3.32g) and 12.5%(3.65g) level and which is followed by P. fluorescens, BCA 1 strain of B. subtilis and Trichoderma viride. Under pot culture conditions, the effect of talc formulated biocontrol agents and challenge inoculation with pathogen was assessed against root rot incidence. It was found that the soil application of TNAU strain of B.subtilis performed better in reducing the root rot incidence at pH of 7.0 (2.37%), 7.5 (4.50%), 8.0 (5.53%) and 8.7 (6.57%) and followed by BCA 1 of B.subtilis in all pH level. Among the biocontrol agents, TNAU strain of B.subtilis applied as seed as well as soil application expressed more population in the rhizosphere in all pH level. The biocontrol agents applied as soil application had more populations of the agents in the soil when compared to seed treatment. The halo tolerance performance of the biocontrol agents was also assessed under field condition in pH of 7.5 and 8.7 during 2019-20 and 2020-21. It was found that the minimum root rot incidence and maximum yield was observed from soil application of TNAU strain of B subtilis at 2.5 kg/ha but the effect is on par with soil application of BCA1 strain of B.subtilis at 2.5 kg/ha.

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Infections of bean plant and field soil are linked to region, root rot pathogen, and agro-ecosystem

Hellenic Plant Protection Journal ◽

10.2478/hppj-2021-0002 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

B. Naseri ◽

M. Gheitury ◽

M. Veisi

Keyword(s):

Root Rot ◽

Multivariate Analyses ◽

Control Method ◽

Control Strategies ◽

Fusarium Species ◽

Macrophomina Phaseolina ◽

Soil Variables ◽

Plant Infection ◽

Pathogen Prevalence ◽

Soil Infestation

SummaryUnderstanding pathogen-agrosystem interaction is particularly essential when applying a control method to minimize pathogen prevalence prior to plant infection. To meet this requirement, frequency of major root rot pathogens isolated from bean root and seed, and their soil populations were examined in farmers’ fields. Multivariate analyses evidenced more frequent isolations of Fusarium solani and Rhizoctonia solani from root and seed compared to Macrophomina phaseolina and Fusarium oxysporum. Two Fusarium species had denser soil populations than R. solani and M. phaseolina. More frequent isolations of pathogens were detected in root and seed collected from Abhar and Khodabandeh compared to Kheirabad region. Agronomic and soil variables corresponded less closely to root infections compared to soil infestation and seed infections. Bean market class, herbicide application, and planting depth were linked to root, seed and soil infestations. Such information provides a basis for increased confidence in choosing appropriate control strategies for a pathogen and region in sustainable agriculture.

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Morphological and molecular identification of Macrophomina phaseolina in the pool of pathogens of sugar beet (Beta vulgaris) root rot : Screening of fungicides for its control

Research on Crops ◽

10.31830/2348-7542.2020.122 ◽

2020 ◽

Vol 21 (4) ◽

Keyword(s):

Sugar Beet ◽

Beta Vulgaris ◽

Molecular Identification ◽

Root Rot ◽

Macrophomina Phaseolina

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First Report of Fusarium oxysporum Causing Root Rot in Ophiopogon bodinieri in China

Plant Disease ◽

10.1094/pdis-07-19-1542-pdn ◽

2020 ◽

Vol 104 (4) ◽

pp. 1254

Author(s):

B. H. Lu ◽

Z. Wang ◽

G. J. Yi ◽

G. W. Tan ◽

F. Zeng ◽

...

Keyword(s):

Fusarium Oxysporum ◽

Root Rot ◽

First Report

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The growth of Easter lily (Lilium longiflorum) as influenced by vesicular–arbuscular mycorrhizal fungi, Fusarium oxysporum, and fertility level

Canadian Journal of Botany ◽

10.1139/b78-331 ◽

1978 ◽

Vol 56 (21) ◽

pp. 2773-2780 ◽

Cited By ~ 10

Author(s):

R. N. Ames ◽

R. G. Linderman

Keyword(s):

Fusarium Oxysporum ◽

Root Rot ◽

Mycorrhizal Fungi ◽

Control Plant ◽

Fertilizer Treatment ◽

Fertility Level ◽

High Inoculum ◽

Easter Lily ◽

Vesicular Arbuscular ◽

Pot Culture

Easter lily bulbs were inoculated in the greenhouse with pot-culture inoculum containing a mixture of four vesicular–arbuscular (VA) mycorrhizal fungi as well as other fungi and bacteria, including pathogens. These organisms had multiplied in association with roots of lily, onion, and clover in pot cultures inoculated with sievings from lily field soils. Growth, as measured by bulb weight gain, root volume, and total leaf area, was determined on lily bulb plants inoculated at two inoculum levels and grown under three fertilizer regimes. Growth of plants inoculated with pot-culture inoculum was less than that of controls, especially in plants given the high inoculum (which included pot-culture plant roots) and the high rate of fertilization. The growth reduction apparently was due to the combined effect of greater incidence of Fusarium oxysporum root rot infections, damage to roots from fertilizer, and lower incidence of VA mycorrhizal infections. More mycorrhizal infections occurred in the low-fertilizer treatment than in the high- or no-fertilizer treatments at both high and low inoculum levels, but more F. oxysporum root rot occurred in the high-inoculum, high-fertilizer treatment.In a second experiment, lily seedlings that lacked bulb nutrient reserves were grown at a low fertilizer level and inoculated with Acaulospora trappei without any pathogens. Mycorrhizal plants were significantly larger than nonmycorrhizal control plants, and their tissues contained more N, P, K, Ca, and Mg than control plant tissues.

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