scholarly journals Clinico-Histopathological Studies in Canine Transmissible Venereal Tumour

2018 ◽  
Vol 7 (11) ◽  
pp. 2714-2719
Author(s):  
Anup Yadav ◽  
Praveen Kumar ◽  
Sandeep Panihar ◽  
Umed Singh Mehra ◽  
N.S. Bugalia ◽  
...  
Keyword(s):  
Histopathological Studies ◽  
Canine Transmissible Venereal Tumour ◽  
Transmissible Venereal Tumour

Treatment of Canine Transmissible Venereal Tumour using Different Surgico-chemotherapeutic Protocols

2021 ◽  
Author(s):  
Nutan Punchkande ◽  
Rukmani Dewangan ◽  
Raju Sharda ◽  
D. Jolhe ◽  
Dhaleshwari Sahu ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Histopathological Examination ◽  
Surgical Excision ◽  
Treatment Protocols ◽  
Post Surgery ◽  
Vincristine Sulphate ◽  
Group A ◽  
Group B ◽  
Canine Transmissible Venereal Tumour ◽  
Transmissible Venereal Tumour

Background: Canine transmissible venereal tumour (CTVT) also known as infectious sarcoma, venereal granuloma, transmissible lymphosarcoma or sticker tumour is usually transmitted through coitus and mainly affects the external genitalia of young sexually matured dogs. Surgery, chemotherapy, radiotherapy and immunotherapy are considered as effective treatment protocols. Therefore, depending upon the availability present study was designed to investigate the efficacy of different surgico-chemotherapeutic protocols for treatment of canine transmissible venereal tumour.Methods: The study was conducted during January 2018 to July 2018 at the Teaching Veterinary Clinical Complex (TVCC) and Department of Veterinary Surgery and Radiology, College of Veterinary Science and A.H., Anjora, Durg (C.G.) on 18 canines of various breed, irrespective of age, sex and divided into three groups consisting 6 animals in each group. Group A was treated with surgical excision of tumour only where as Group B and Group C were treated with surgical excision of tumour followed by administration of Doxorubicin (30mg/m2) BSA and Vincristine sulphate (0.025 mg/kg) intravenously alongwith DNS at 7th and 14th post-operative days respectively. Different physiological and haemato-biochemical parameters (Hb, PCV, TLC, TPC, DLC, serum glucose, TSP, SUN, SC, ALT, AST and ALP) were recorded preoperatively, postoperatively and after chemotherapy at 10th, 30th and 60th days intervals.Result: The present investigation showed transient changes in physiological and haemato-biochemical parameters before, post surgery and post chemotherapeutic management and was within normal range. Histopathological examination revealed confluent sheet of tumour cells arranged in large round oval or polyhedral shaped distributed in tight clusters or cords. Group A showed mild to moderated reoccurrence while Group B showed minimum reoccurrence. Group C showed no reoccurrence. Thus, surgery combined with vincristine therapy is most effective for treating dogs suffering with transmissible venereal tumour.

scholarly journals Evaluation of a genetic assay for canine transmissible venereal tumour diagnosis in Brazil

2016 ◽  
Vol 15 (2) ◽  
pp. 615-618 ◽  
Author(s):  
K. F. Castro ◽  
A. Strakova ◽  
M. Tinucci-Costa ◽  
E. P. Murchison
Keyword(s):  
Tumour Diagnosis ◽  
Canine Transmissible Venereal Tumour ◽  
Transmissible Venereal Tumour ◽  
Genetic Assay

scholarly journals Nasal Transmissible Venereal Tumor In A Jack Russel Terrier Bitch

2015 ◽  
Vol 65 (1) ◽  
pp. 143-148 ◽  
Author(s):  
FEGHIU Adrian ◽  
CRÎNGANU Dan ◽  
DUMITRESCU Florin ◽  
PASCAL Manuela ◽  
VASILESCU Florina ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Veterinary Medicine ◽  
Nasal Cavity ◽  
Tumor Tissue ◽  
Left Nasal Cavity ◽  
Vincristine Sulphate ◽  
Positive Immunoreactivity ◽  
Canine Transmissible Venereal Tumour ◽  
Transmissible Venereal Tumour ◽  
Primary Form

Abstract A two-year old Jack Russell Terrier female with left-sided epistaxis was brought to the Clinic of the Bucharest Faculty of Veterinary Medicine. Endoscopy of the left nasal cavity revealed a cauliflower-like mass. Cytological and histopathological features were specific to canine transmissible venereal tumour (CTVT). Tumor tissue showed positive immunoreactivity to anti-vimentin monoclonal antibody (90%) and Ki67 (35%) and samples were negative for anti-cytokeratin monoclonal antibody. Chemotherapy with vincristine sulphate at a dose of 0.025 mg/kg i.v./week for 6 administrations was successful. This paper presents a primary form of nasal CTVT in a Jack Russell Terrier female.

scholarly journals Evaluation of a canine transmissible venereal tumour cell line with tumour immunity capacity but without tumorigenic property

2019 ◽  
Vol 63 (2) ◽  
pp. 225-233 ◽  
Author(s):  
Yareellys Ramos Zayas ◽  
Moisés Armides Franco Molina ◽  
Reyes Tamez Guerra ◽  
Cristina Rodríguez Padilla
Keyword(s):  
Cell Line ◽  
Financial Burden ◽  
Morphological Characteristics ◽  
Tumour Cells ◽  
Tumour Cell Line ◽  
Histopathological Analysis ◽  
Isolated Cells ◽  
Tumour Immunity ◽  
Canine Transmissible Venereal Tumour ◽  
Transmissible Venereal Tumour

AbstractIntroduction:Canine transmissible venereal tumour (CTVT) is a sexually transmitted tumour affecting dogs worldwide, imposing a financial burden on dog owners. A stable culture cell line in continuous passages for >18 months has only been achieved once. The present study investigated a stable CTVT cell line isolated from a bitch and its potential as a vaccine.Material and Methods:A biopsy from a 2-year-old mongrel bitch with CTVT was obtained for histopathological confirmation and isolation of tumour cells. The isolated cells were cultured to passage 55 and characterised by flow cytometry, with karyotyping by GTG-banding and by PCR detection of myc S-2 and LINE AS1. The isolated CTVT cell line was also used as a preventive vaccine in a canine model.Results:Histopathological analysis of the isolated tumour cells revealed typical CTVT characteristics. Constant proliferation and stable morphological characteristics were observed during culture. Phenotypic analysis determined the expression of HLA-DR+, CD5.1+, CD14+, CD45+, CD83+, CD163+, and Ly-6G-Ly-6C+. GTG-banding revealed a mean of 57 chromosomes in the karyotype with several complex chromosomal rearrangements. LINE-c-myc insertion in the isolated CTVT cell line at 550 bp was not detected. However, a 340-bp band was amplified. Isolated CTVT cell line inoculation at a concentration of 1×108did not induce tumour growth in bitches, nor did a challenge with primary CTVT cells.Conclusion:The present study successfully identified and isolated a stable CTVT cell line that may be useful in CTVT prevention.

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