scholarly journals Comparative efficacy of different methods in the generation of 12S particles from 146S particles of FMDV serotype A and their detection using serotype (146S) specific monoclonal antibodies

2020 ◽  
Vol 9 (2) ◽  
pp. 1016-1026
Author(s):  
S. Shanmuganathan ◽  
Gollapalli Sivarama Krishna ◽  
S.R. Nagasupreeta ◽  
K. Kavya ◽  
Shreya Gopinath ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Comparative Efficacy ◽  
Serotype A ◽  
Fmdv Serotype

Comparative gene genealogical analyses of strains of serotype AD identify recombination in populations of serotypes A and D in the human pathogenic yeast Cryptococcus neoformans

Microbiology ◽  
2003 ◽  
Vol 149 (8) ◽  
pp. 2147-2154 ◽  
Author(s):  
Jianping Xu ◽  
Thomas G. Mitchell
Keyword(s):  
Monoclonal Antibodies ◽  
Cryptococcus Neoformans ◽  
Natural Populations ◽  
The Other ◽  
Pathogenic Yeast ◽  
Gene Genealogy ◽  
Serotype A ◽  
The World ◽  
Significant Divergence ◽  
Allelic Group

Cryptococcus neoformans is a major pathogen of humans throughout the world. Using commercial monoclonal antibodies to capsular epitopes, strains of C. neoformans manifest five serotypes: A, B, C, D and AD. Previous studies demonstrated significant divergence among serotypes A, B, C and D, which are typically haploid. In contrast, most strains of serotype AD are diploid or aneuploid and result from recent hybridization between strains of serotypes A and D. Whether serotypes A, B, C and D represent strictly asexual lineages is not known. Using comparative genealogical analyses of two genes, the authors investigated whether recombination occurred among strains within serotypes A and D. For each of 14 serotype AD strains, a portion (642 bp) of the orotidine monophosphate pyrophosphorylase (URA5) gene was cloned and sequenced. Each of these 14 strains contained two different alleles and sequences for both alleles were obtained. The URA5 gene genealogy was compared to that derived from the laccase (LAC) gene, which was reported recently for the same 14 strains. For both genes, each of the 14 serotype AD strains contained two phylogenetically distinct alleles: one allele was highly similar to those from serotype A strains and the other to alleles from serotype D strains. However, within both the serotype A allelic group and the serotype D allelic group, there was significant incongruence between genealogies derived from URA5 and LAC. The results suggest recombination in natural populations of both serotypes A and D.

scholarly journals Monoclonal Antibodies Reactive with Immunorecessive Epitopes of Glucuronoxylomannan, the Major Capsular Polysaccharide of Cryptococcus neoformans

2003 ◽  
Vol 10 (5) ◽  
pp. 903-909 ◽  
Author(s):  
Suzanne Brandt ◽  
Peter Thorkildson ◽  
Thomas R. Kozel
Keyword(s):  
Monoclonal Antibody ◽  
Immune Response ◽  
Monoclonal Antibodies ◽  
Cryptococcus Neoformans ◽  
Capsular Polysaccharide ◽  
Passive Immunization ◽  
The Other ◽  
Serotype A ◽  
Humoral Immune ◽  
New Information

ABSTRACT Cryptococcus neoformans is surrounded by an antiphagocytic capsule whose primary constituent is glucuronoxylomannan (GXM). An epitope shared by GXM serotypes A, B, C, and D is immunodominant when mice are immunized with serotype A GXM. In contrast, an epitope shared only by serotypes A and D is immunodominant when mice are immunized with serotype D. Hybridomas secreting antibodies reactive with subdominant epitopes were identified through a positive-negative screening procedure in which antibody-secreting colonies were characterized by reactivity with both the immunizing polysaccharide and GXMs from each of the four major serotypes. In this manner, a monoclonal antibody (MAb) that was reactive with an epitope shared only by serotypes A and B was identified and designated F10F5. Such an epitope has not been described previously. Immunization of mice with de-O-acetylated serotype A GXM generated a hybridoma that secreted an antibody, designated F12D2, that was reactive with all four serotypes. Unlike previously described monoclonal and polyclonal panspecific antibodies, the reactivity of MAb F12D2 was not altered by de-O-acetylation of GXM. These results indicate that there are at least two panspecific GXM epitopes; one epitope is dependent on O acetylation for antibody reactivity, and the other is independent of O acetylation. This study identifies strategies for production of MAbs that are reactive with subdominant or cryptic GXM epitopes and provides new information regarding the antigenic makeup and the humoral immune response to GXM, an essential virulence factor that is a target for active and passive immunization.

Preparation and Characterization of Neutralizing Monoclonal Antibodies Against FMDV Serotype O with Synthetic Peptide Antigen

Hybridoma ◽  
2010 ◽  
Vol 29 (5) ◽  
pp. 409-412 ◽  
Author(s):  
Lina Ma ◽  
Yong-sheng Liu ◽  
Yao-zhong Ding ◽  
Hao-tai Chen ◽  
Jian-hua Zhou ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Synthetic Peptide ◽  
Peptide Antigen ◽  
Serotype O ◽  
Fmdv Serotype

scholarly journals Monoclonal Antibodies Targeting the Alpha-Exosite of Botulinum Neurotoxin Serotype/A Inhibit Catalytic Activity

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0135306 ◽  
Author(s):  
Yongfeng Fan ◽  
Isin N. Geren ◽  
Jianbo Dong ◽  
Jianlong Lou ◽  
Weihua Wen ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Catalytic Activity ◽  
Botulinum Neurotoxin ◽  
Serotype A ◽  
Botulinum Neurotoxin Serotype A

Monoclonal antibodies to the glycoprotein of vesicular stomatitis virus (New Jersey serotype): A method for preliminary mapping of epitopes

Virology ◽  
1987 ◽  
Vol 161 (2) ◽  
pp. 533-540 ◽  
Author(s):  
Betsy J. Bricker ◽  
Ruth M. Snyder ◽  
Jay W. Fox ◽  
Wesley A. Volk ◽  
Robert R. Wagner
Keyword(s):  
Monoclonal Antibodies ◽  
New Jersey ◽  
Vesicular Stomatitis Virus ◽  
Serotype A ◽  
Vesicular Stomatitis

Preparation of monoclonal antibodies reactive with beta-1,2-linked oligomannosyl residues in the phosphomannan-protein complex of Candida albicans NIH B-792 strain.

1988 ◽  
Vol 34 (3) ◽  
pp. 539-543 ◽  
Author(s):  
M Tojo ◽  
N Shibata ◽  
M Kobayashi ◽  
T Mikami ◽  
M Suzuki ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Candida Albicans ◽  
Protein Complex ◽  
Protein Complexes ◽  
Myeloma Cell ◽  
Polyclonal Antiserum ◽  
Ascites Fluid ◽  
Serotype A ◽  
Mouse Myeloma Cell Line ◽  
Serotype B

Abstract Hybridomas obtained by fusing the spleen cells of BALB/c female mice hyperimmunized with heat-killed yeast-form cells of Candida albicans NIH B-792 strain and a mouse myeloma cell line, P3X63Ag8.653, produced antibodies to beta-1,2-linked oligomannosyl residues in the phosphomannan-protein complex of the parent cells. Most of these monoclonal antibodies were IgM, but about 10% of the hybridomas produced IgG1 immunoglobulins. Ascites fluid from BALB/c mice inoculated with an IgG1-producing hybridoma showed different precipitability with the phosphomannan-protein complexes of three representative C. albicans strains, with NIH B-792 (serotype B) greater than NIH A-207 (serotype A) greater than J-1012 (serotype A, formerly serotype C). In contrast, a rabbit polyclonal antiserum to C. albicans NIH B-792 cells was unable to distinguish these same complexes. This ascites fluid agglutinated the heat-killed cells of three Candida strains, but not those of three others or of Torulopsis glabrata IFO 0622. The other ascites fluids, containing antibodies of the IgM class, agglutinated cells from three C. albicans strains and also C. tropicalis IFO 0587 cells.

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