Cucumis sativus is one of the most important vegetable crop being grown in Pakistan, cucumber is being affected by different types of pathogens and downy mildew is one of the detrimental disease causing losses upto 40%. RCBD design was used for fungicide evaluation, Varieties viz., beithoalfa, Marketmore-76, Cucumber 363, Anmol , Marketmore and Desi were evaluated it was found that all varieties were showed positive response in minimizing disease disease out of which variety Beithoalfa showed the most effective control of disease followed by Marketmore-76, Cucumber 363, Anmol, Marketmore and Desi/Control. Among the treatment of fungicides: Moral fungicide showed most effective control of disease followed by Agrohut, Acute, Antracol, Cabriotop and Control, out of all fungicides “cabriotop” showed the least effective results against downy mildew disease.