scholarly journals Simulation Study of Size-structure Dynamics with Changing Spatial Pattern of Tree Sizes in a Lattice-planted Japanese Cedar (Cryptomeria japonica D. Don) Plantation

2009 ◽  
Vol 15 (1) ◽  
pp. 11-19
Author(s):  
Sumihiro Inoue ◽  
Tetsuoh Shirota ◽  
Yasushi Mitsuda ◽  
Hiroaki Ishii ◽  
Koichiro Gyokusen
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yoshihiko Nanasato ◽  
Masafumi Mikami ◽  
Norihiro Futamura ◽  
Masaki Endo ◽  
Mitsuru Nishiguchi ◽  
...  

AbstractCryptomeria japonica (Japanese cedar or sugi) is one of the most important coniferous tree species in Japan and breeding programs for this species have been launched since 1950s. Genome editing technology can be used to shorten the breeding period. In this study, we performed targeted mutagenesis using the CRISPR/Cas9 system in C. japonica. First, the CRISPR/Cas9 system was tested using green fluorescent protein (GFP)-expressing transgenic embryogenic tissue lines. Knock-out efficiency of GFP ranged from 3.1 to 41.4% depending on U6 promoters and target sequences. The GFP knock-out region was mottled in many lines, indicating genome editing in individual cells. However, in 101 of 102 mutated individuals (> 99%) from 6 GFP knock-out lines, embryos had a single mutation pattern. Next, we knocked out the endogenous C. japonica magnesium chelatase subunit I (CjChlI) gene using two guide RNA targets. Green, pale green, and albino phenotypes were obtained in the gene-edited cell lines. Sequence analysis revealed random deletions, insertions, and replacements in the target region. Thus, targeted mutagenesis using the CRISPR/Cas9 system can be used to modify the C. japonica genome.


Oecologia ◽  
2015 ◽  
Vol 179 (2) ◽  
pp. 425-433 ◽  
Author(s):  
Asaf Sadeh ◽  
Antonina Polevikov ◽  
Marc Mangel ◽  
Leon Blaustein

2015 ◽  
Vol 7 (3) ◽  
pp. 295 ◽  
Author(s):  
Jaechun Lee ◽  
Keun Hwa Lee ◽  
Hye-Sook Lee ◽  
Sung-Chul Hong ◽  
Jeong Hong Kim

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