Isolation and Characterization of Polymorphic Microsatellite <em>loci</em> from the Invasive Worm <em>Branchiomma luctuosum</em> (Grube, 1870) (Annelida: Sabellidae)

History Of ◽

Polymorphic Microsatellite ◽

High Level ◽

Activity Areas

Introduction of exotic species in new areas through anthropic action is one of the major problems that can affect biodiversity. Branchiomma luctuosum is known for its highly invasive potential and the actual occurrence of species commonly associated with port activity areas is an extra evidence that this anthropogenic activity should not be underestimated. In order to develop suitable molecular markers for future studies on colonization routes and population dynamics of the invading individuals of B. luctuosum, nine highly polymorphic microsatellite loci were isolated and their polymorphism levels were evaluated. These loci showed a range of number of alleles per locus from five to ten and all loci had a high level of genetic diversity, and exhibited significant heterozygote deficiencies probably due to the presence of null alleles. Significant deviations from the Hardy-Weinberg equilibrium were detected at several loci and most of them were related to a heterozygous deficit. Heterozygous deficiency can be expected in this case due to the biology and history of this invasive species, in relation to its recent introduction in Brazilian coast and possible action of multiple introductory events.

Isolation and Characterization of Polymorphic Microsatellite loci from the Invasive Worm Branchiomma luctuosum (Grube, 1870) (Annelida: Sabellidae)

10.20944/preprints201808.0409.v2 ◽

2018 ◽

Author(s):

Flávia Ariany Belato ◽

Elisa Maria Costa-Paiva ◽

Paulo Cesar Paiva ◽

Anderson Vilasboa

Keyword(s):

Null Alleles ◽

Anthropogenic Activity ◽

Brazilian Coast ◽

History Of ◽

Polymorphic Microsatellite ◽

High Level ◽

Activity Areas

Introduction of exotic species in new areas through anthropic action is one of the major problems that can affect biodiversity. Branchiomma luctuosum is known for its highly invasive potential and the actual occurrence of species commonly associated with port activity areas is an extra evidence that this anthropogenic activity should not be underestimated. In order to develop suitable molecular markers for future studies on colonization routes and population dynamics of the invading individuals of B. luctuosum, nine highly polymorphic microsatellite loci were isolated and their polymorphism levels were evaluated. These loci showed a range of number of alleles per locus from five to ten and all loci had a high level of genetic diversity, and exhibited significant heterozygote deficiencies probably due to the presence of null alleles. Significant deviations from the Hardy-Weinberg equilibrium were detected at several loci and most of them were related to a heterozygous deficit. Heterozygous deficiency can be expected in this case due to the biology and history of this invasive species, in relation to its recent introduction in Brazilian coast and possible action of multiple introductory events.

Isolation and Characterization of Twenty Polymorphic Microsatellite Loci for Moringa oleifera (Moringaceae)

HortScience ◽

10.21273/hortsci.45.4.690 ◽

2010 ◽

Vol 45 (4) ◽

pp. 690-692 ◽

Cited By ~ 7

Author(s):

Jiang-Chong Wu ◽

Jing Yang ◽

Zhi-Jian Gu ◽

Yan-Ping Zhang

Keyword(s):

Microsatellite Markers ◽

Microsatellite Loci ◽

Population Genetic ◽

Moringa Oleifera ◽

Microsatellite Primers ◽

Genetic Studies ◽

Polymorphic Microsatellite

By using a modified biotin-streptavidin capturing method, a total of 20 polymorphic microsatellite markers were developed from Moringa oleifera Lam. (Moringaceae), a useful multipurpose tree. Twenty-four domesticated individuals, with germplasms of India and Myanmar, were used to screen polymorphism of these 20 microsatellite markers. The number of alleles per locus ranged from two to six. The expected and observed heterozygosity varied from 0.3608 to 0.7606 and from 0.0000 to 0.8750, respectively. Seven loci were significantly deviated from Hardy-Weinberg equilibrium. The availability of these microsatellite primers would provide a powerful tool for aspects of detailed population genetic studies of M. oleifera.

Development and characterization of new polymorphic microsatellite loci for Trionyx triunguis (Testudines: Trionychidae) in the Mediterranean Basin

Amphibia-Reptilia ◽

10.1163/15685381-00003003 ◽

2015 ◽

Vol 36 (3) ◽

pp. 318-324 ◽

Cited By ~ 1

Author(s):

Özgür Güçlü ◽

Salih Hakan Durmuş ◽

Kamil Candan ◽

Nurettin Beşer ◽

Süheyla Türkyilmaz ◽

...

Keyword(s):

Microsatellite Loci ◽

Captive Breeding ◽

Null Alleles ◽

Enriched Library ◽

Polymorphic Microsatellite ◽

The Mediterranean ◽

Captive Breeding Programs

The Mediterranean subpopulation of T. triunguis has been listed by IUCN as critically endangered (European Reptile and Amphibian Specialist Group 1996, category CR C2A). There are very few studies for determination of distribution and population genetics of this species in the world. A total of 13 polymorphic microsatellite loci were isolated from 42 samples of T. triunguis by using biotinylated (GT)10, (CT)10, (GATA)5-enriched library. The range of observed heterozygosities was 0.000-0.833 with a mean of 0.274, and the range of expected heterozygosities was 0.081-0.607 with a mean of 0.374. It was found that four loci deviated significantly from Hardy-Weinberg equilibrium after Bonferroni correction analysis. There was no evidence for large allele dropout. Null alleles may be present at ttmed-6, ttmed-8, and ttmed-9 loci, as suggested by the general excess of homozygote for most allele size classes. Designed microsatellite loci could provide a powerful tool for the population structure and conservation genetics studies of the Nile soft-shelled turtle and for captive breeding programs or to detect species illegal trade.

Isolation and Characterization of 17 Polymorphic Microsatellite Loci for Musella lasiocarpa (Musaceae)

HortScience ◽

10.21273/hortsci.44.7.2041 ◽

2009 ◽

Vol 44 (7) ◽

pp. 2041-2042 ◽

Cited By ~ 2

Author(s):

Chun-yan Yang ◽

Yuan Huang ◽

Chunlin Long

Keyword(s):

Genetic Diversity ◽

Population Structure ◽

Southwest China ◽

Microsatellite Primers ◽

Genetic Population ◽

Allele Number ◽

Polymorphic Microsatellite

Seventeen microsatellite markers were isolated from 24 wild individuals collected from three populations of Musella lasiocarpa (Franch.) C. Y. Wu ex H. W. Li, a plant endemic to southwest China. The average allele number of these markers was 3.6 per locus, ranging from two to seven. The expected (He) and observed (Ho) heterozygosities varied from 0.4672 to 0.8183 and from 0.0417 to 1, respectively. Eleven loci were significantly deviated from Hardy-Weinberg equilibrium as a result of the heterozygote deficiency. These microsatellite primers will be used for studying genetic population structure and genetic diversity of M. lasiocarpa.

Isolation and Characterization of Polymorphic Microsatellite Markers for Two Subterranean Termites

Sociobiology ◽

10.13102/sociobiology.v64i3.1692 ◽

2017 ◽

Vol 64 (3) ◽

pp. 352 ◽

Cited By ~ 1

Author(s):

Yu-Lei Dang ◽

Hong-Gui Zhang ◽

Yu-Feng Meng ◽

Min Zhang ◽

Sha Zhao ◽

...

Keyword(s):

Microsatellite Markers ◽

Microsatellite Loci ◽

Population Genetic ◽

Subterranean Termites ◽

Polymorphic Markers ◽

Expected Heterozygosity ◽

Polymorphic Microsatellite

We isolated 15 and 18 highly polymorphic genomic microsatellite markers from two subterranean termites, Reticulitermes aculabialis and R. labralis, respectively. A total of 53 alleles were detected in 15 microsatellite loci of R. aculabialis, and the alleles were 3.533±1.302 (mean±SD), while the corresponding data of R. labralis were 115 detected alleles in 18 microsatellite loci with 6.389±1.754 alleles. The observed and expected heterozygosity was 0.496±0.236 and 0.564±0.125 in R. aculabialis, and 0.368±0.263 and 0.702±0.115 in R. labralis, respectively. Seven loci were highly polymorphic (PIC>0.5) in R. aculabialis, and 15 loci were highly polymorphic (PIC>0.5) in R. labralis. All loci showed Hardy–Weinberg equilibrium. These polymorphic markers provide useful tools for population genetic and breeding system studies of subterranean termites.

Isolation and Characterization of Microsatellite Markers for Soybean Looper (Lepidoptera: Noctuidae)

Journal of Insect Science ◽

10.1093/jisesa/iez101 ◽

2019 ◽

Vol 19 (5) ◽

Author(s):

C S Silva ◽

E M G Cordeiro ◽

A S Corrêa

Keyword(s):

Population Structure ◽

Microsatellite Markers ◽

Null Alleles ◽

High Degree ◽

Soybean Looper ◽

Lepidoptera Noctuidae ◽

F Statistics

Abstract We constructed 13 microsatellite markers for Chrysodeixis includens (Walker), a serious crop pest in the Americas. All SSR (Simple Sequence Repeat) markers were polymorphic, with no evidence of linkage disequilibrium between any loci in any population. The total number of alleles per locus ranged from 5 for L3, L9, and L11 to 26 for L6; the mean number of alleles per locus in three populations of C. includens ranged from 2.33 for L3 to 14.67 for L6. Hardy–Weinberg equilibrium (HWE) deviation was not observed in four loci for at least one population (L3, L5, L9, L10). Markers L6, L7, L8, L10, L11, L12, and L13 showed a frequency of null alleles > 0.2 for at least one population. STRUCTURE and F-statistics revealed low population structure among the populations (FST = 0.013) and a high degree of inbreeding (FIS = 0.658). The SSR markers developed here will be useful in future studies on the ecology, demography, host dynamics, and gene flow of C. includens. This information is essential to understand the recent status of C. includens as a key pest in South America.