scholarly journals The Influence of Probiotics of Different Microbiological Composition on Histological Structure of the Organs of the Gastrointestinal Tract of Juvenile Oncorhynchus mykiss

2021 ◽  
Author(s):  
Alexei L. Nikiforov-Nikishin ◽  
Dimitri L. Nikiforov-Nikishin ◽  
Nikita I. Kochetkov ◽  
Svetlana V. Smorodinskaya ◽  
Victor A. Klimov
Keyword(s):  
Bacillus Subtilis ◽  
Oncorhynchus Mykiss ◽  
Intestinal Tract ◽  
Secretory Activity ◽  
Goblet Cells ◽  
Morphometric Parameters ◽  
Fish Growth ◽  
Histological Structure ◽  
Normal Functional ◽  
Gastro Intestinal Tract

This paper studies the influence of three probiotic preparations of various microbiological composition: Bacillus subtilis (O1); Bacillus subtilis + Bacillus amyloliquefaciens (O2); Lactobacil-lus acidophilus (O3) on the growth process and histological structure of the organs of the gastroin-testinal tract of juvenile Oncorhynchus mykiss by morphometric parameters. The effect of the pro-biotic preparations led to the increase in fish growth and influenced different sections of the gastro-intestinal tract. The biggest change was found in the mid intestine and the reliable difference com-pared to the control was obtained at the following parameters: Lamina propria width, intraepithe-lial lymphocytes number of prismatic epithelium and goblet cells area. The changes in the pyloric appendages were less obvious but reported as playing an important functional role. The liver pre-served normal functional structure in all series of the experiment except for the probiotic group Lactobacillus acidophilus, where hepatocyte small-drop vacuolization was observed. That might be connected with the change of the digest activity resulting from a decrease in secretory activity of the intestinal exocrinocytes. The use of all the probiotic preparations led to the similar change in morphometric parameters, in all the groups it was possible to decrease the immune response.

scholarly journals Nutrition Physiology of Juveniles Individuals of Pumpkinseed Lepomis gibbosus (Linnaeus, 1758) from the Samara Bay (Ukraine)

2021 ◽  
Vol 83 ◽  
pp. 15-21
Author(s):  
O.S. Nesterenko ◽  
Oleg M. Marenkov ◽  
Ye.S. Pylypenko
Keyword(s):  
Intestinal Epithelium ◽  
Digestive System ◽  
Scientific Research ◽  
Goblet Cells ◽  
Morphometric Parameters ◽  
Fish Nutrition ◽  
Lepomis Gibbosus ◽  
Histological Structure ◽  
Research Results ◽  
June July

The article presents the results of studies physiology of nutrition of juveniles individuals of pumpkinseed Lepomis gibbosus (Linnaeus, 1758) from the Zaporizhzhia (Dnipro) reservoir. histological structure, morphometric parameters of digestive system and nutrition biology were investigated. The material for work were collected during scientific research in June-July 2016–2017. The ichthyological sampling was carried out in the Samara Bay of the Zaporizhzhia (Dnipro) reservoir. Researches of structure of digestive system were conducted using histological methods, researches of nutrition were conducted using ichthyological and hydrobiological methods The height of the intestinal epithelium of the pumpkinseed`s juveniles varies from 15.5 microns to 51.9 microns and on average is 32.59 ± 9.03 microns, the area of secretory goblet cells of the intestine reaches 198.61 ± 36.55 μm2. It was revealed that the pumkinseed’s juveniles only feed on phytoplankton. Research results can be useful for further study on the fish nutrition, especially sexual matured. Also, nutrition studies can help to find biomeliorative methods to combat the pumpkinseed, because the species is in competition with industrially valuable species of fish for fodder.

Non Steroidal Anti-Inflammatory Drug Induced Damage on Lower Gastro-Intestinal Tract: Is There an Involvement of Microbiota?

2014 ◽  
Vol 9 (3) ◽  
pp. 196-204 ◽  
Author(s):  
Lucia Montenegro ◽  
Giuseppe Losurdo ◽  
Raffaele Licinio ◽  
Maria Zamparella ◽  
Floriana Giorgio ◽  
...  
Keyword(s):  
Intestinal Tract ◽  
Drug Induced ◽  
Inflammatory Drug ◽  
Anti Inflammatory ◽  
Gastro Intestinal Tract

scholarly journals Rehydration before Application Improves Functional Properties of Lyophilized Lactiplantibacillus plantarum HAC03

2021 ◽  
Vol 9 (5) ◽  
pp. 1013
Author(s):  
Karina Arellano-Ayala ◽  
Juhwan Lim ◽  
Subin Yeo ◽  
Jorge Enrique Vazquez Bucheli ◽  
Svetoslav Dimitrov Todorov ◽  
...  
Keyword(s):  
Intestinal Tract ◽  
Food Additives ◽  
Nitrogen Compounds ◽  
Protein Nitrogen ◽  
Bacterial Surface ◽  
Freeze Dried ◽  
Immunomodulatory Properties ◽  
Gastro Intestinal Tract ◽  
The Impact ◽  
Active Formulation

Preservation of probiotics by lyophilization is considered a method of choice for developing stable products. However, both direct consumption and reconstitution of dehydrated probiotic preparations before application “compromise” the survival and functional characteristics of the microorganisms under the stress of the upper gastro-intestinal tract. We evaluated the impact of different food additives on the viability, mucin adhesion, and zeta potential of a freeze-dried putative probiotic, Lactiplantibacillus (Lp.) plantarum HAC03. HAC03-compatible ingredients for the formulation of ten rehydration mixtures could be selected. Elevated efficacy was achieved by the B-active formulation, a mixture of non-protein nitrogen compounds, sugars, and salts. The survival of Lp. plantarum HAC03 increased by 36.36% compared rehydration with distilled water (4.92%) after passing simulated gastro-intestinal stress conditions. Cell viability determined by plate counting was confirmed by flow cytometry. B-active formulation also influenced Lp. plantarum HAC03 functionality by increasing its adherence to a Caco-2 cell-line and by changing the bacterial surface charge, measured as zeta potential.Hydrophobicity, mucin adhesion and immunomodulatory properties of Lp. plantarum HAC03 were not affected by the B-active formulation. The rehydration medium also effectively protected Lp. plantarum ATCC14917, Lp. plantarum 299v, Latilactobacillus sakei (Lt.) HAC11, Lacticaseibacillus (Lc.) paracasei 532, Enterococcus faecium 200, and Lc. rhamnosus BFE5263.

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