Area Under the Curve Spectrophotometric Method for Determination of Irbesatran in Pharmaceutical Formulation

<em>This study presents new spectrophotometric method for the determination of Irbesartan. So far, no Area under Curve Spectrophotometric method has been reported for the estimation of Irbesartan from pharmaceutical dosage form. This paper deals with validation and development of a method by Area Under Curve for the assay of Irbesartan from pharmaceutical dosage forms. The principle for AUC curve method is “the area under two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The area selected between 203 to 211 nm for determination of Irbesartan. The drug follows Beer-Lambert’s law over the concentration range of 5-25 μg/ml for Irbesartan. In accuracy study the % recovery of Irbesartan in bulk drug samples was ranged 96.45-93.84%, which indicates that the method was accurate. Validation of the proposed method was carried out for its accuracy, precision, and specificity according to ICH guidelines. The proposed methods can be successfully applied in routine work for the determination of Tigecycline in its pharmaceutical dosage form.</em>

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Spectrophotometric analysis of Azithromycin and its pharmaceutical dosage forms: Comparison between Spectrophotometry and HPLC

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v12i2.21981 ◽

2015 ◽

Vol 12 (2) ◽

pp. 171-179 ◽

Cited By ~ 3

Author(s):

Nahid Sharmin ◽

Nazia Sultana Shanta ◽

Sitesh C Bachar

Keyword(s):

Statistical Analysis ◽

Spectrophotometric Method ◽

Dosage Form ◽

Dosage Forms ◽

Spectrophotometric Analysis ◽

Pharmaceutical Dosage Form ◽

Pharmaceutical Dosage Forms ◽

Ich Guidelines ◽

Good Accordance

A simple, reliable, precise and sensitive UV-spectrophotometric method was developed and validated for the estimation of azithromycin in pharmaceutical dosage form and compared with official USP 2010 method. The proposed method utilizes the oxidation of azithromycin with potassium permanganate to liberate formaldehyde. This formaldehyde reacts with acetone-ammonium reagent and produces yellow colored chromogen 3,5-diacetyl-2,6-dihydrolutidine. The colored solution exhibited a maximum absorption at 412 nm which can be detected with UVspectrophotometer. The method was found linear over the concentration range 80% to 120% of the working concentration (R2=0.999). The intra- and inter-day RSD (n = 6) was ? 2.0%. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. The proposed method was successfully applied for determination of azithromycin and the results have been compared with HPLC and thus enabling the utility of this new method for routine analysis azithromycin in pharmaceutical dosage forms DOI: http://dx.doi.org/10.3329/dujps.v12i2.21981 Dhaka Univ. J. Pharm. Sci. 12(2): 171-179, 2013 (December)

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Development and Validation of a Stability Indicating RP-HPLC Method for the Determination of Imatinib Mesylate

International Journal of Current Pharmaceutical Review and Research ◽

10.25258/ijcprr.v8i01.9091 ◽

2017 ◽

Vol 8 (01) ◽

Author(s):

Y. Rajesh Babu ◽

N. Appalaraju

Keyword(s):

Imatinib Mesylate ◽

Dosage Form ◽

Hplc Method ◽

Pharmaceutical Dosage Form ◽

Pharmaceutical Dosage Forms ◽

Stability Indicating ◽

Rp Hplc ◽

Bulk Drug ◽

The Stability

The aim of this paper was to develop and validate the stability indicating RP-HPLC method for the determination of Imatinib mesylate in bulk and pharmaceutical dosage forms. A simple, accurate, precise, sensitive and stability indicating RP-HPLC method has been developed for the determination of Imatinib mesylate in bulk drug and pharmaceutical dosage form, in which separations are done using develosil C18, 5μm, 150 × 4.6mm i.d. column at a flow rate of 1.0mL/min with an injection volume of 20μL. The beer’s law was obeyed over the concentration range of 5 - 35μg/mL. The correlation coefficient was found to be 0.996 and it showed good linearity, reproducibility, precision in this concentration range. The % recovery values were found to be within the limits, which showed that the method was accurate. The LOD and LOQ were calculated using statistical methods. The % RSD values were less than 2. The developed method was successfully applied for determination of Imatinib mesylate in pharmaceutical dosage form. The results obtained are in good agreement with those obtained by using the standard method.

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Spectrophotometric Estimation of Amoxicillin Trihydrate in Bulk and Pharmaceutical Dosage Form

E-Journal of Chemistry ◽

10.1155/2008/350646 ◽

2008 ◽

Vol 5 (s2) ◽

pp. 1114-1116 ◽

Cited By ~ 3

Author(s):

K. Prakash ◽

P. Narayana Raju ◽

K. Shanta Kumari ◽

M. Lakshmi Narasu

Keyword(s):

Correlation Coefficient ◽

Spectrophotometric Method ◽

Phosphate Buffer ◽

Dosage Form ◽

Molar Absorptivity ◽

Pharmaceutical Dosage Form ◽

Amoxicillin Trihydrate ◽

Bulk Drug ◽

Tablet Dosage

New simple, sensitive, rapid, reproducible and economical spectrophotometric method have been developed for the determination of amoxicillin trihydrate in pharmaceutical bulk and tablet dosage form using citro phosphate buffer pH 7.2. The system obeys Lambert-Beer's law at 231 nm in the concentration range 2.5-50 μg/m. Molar absorptivity, correlation coefficient and Sandell's sensitivity values were found to be 1.0020 x 104mol-1cm,-10.9996 and 0.03906 μg cm-2respectively. The proposed methods have been successfully applied to the analysis of the bulk drug and its tablet dosage form. The methods have been statistically evaluated and were found to be precise and accurate

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New RP-HPLC Method Development and Validation for the Determination of Pazopanib Hydrochloride in Tablet Dosage Form

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.v8i1.8434 ◽

2017 ◽

Vol 8 (1) ◽

Author(s):

Rajesh Babu Y. ◽

Appala Raju N.

Keyword(s):

Dosage Form ◽

Method Development ◽

Hplc Method ◽

Pharmaceutical Dosage Form ◽

Pharmaceutical Dosage Forms ◽

Stability Indicating ◽

Rp Hplc ◽

Bulk Drug ◽

The Stability

The aim of this paper was to develop and validate the stability indicating RP-HPLC method for the determination of Pazopanib hydrochloride in bulk and pharmaceutical dosage forms. A simple, accurate, precise, sensitive and stability indicating RP-HPLC method has been developed for the determination of Pazopanib hydrochloride in bulk drug and pharmaceutical dosage form, in which separations are done using develosil C18, 5μm, 150 × 4.6mm i.d. column at a flow rate of 1.0mL/min with an injection volume of 20μL. The beer’s law was obeyed over the concentration range of 5 - 35μg/mL. The correlation coefficient was found to be 0.996 and it showed good linearity, reproducibility, precision in this concentration range. The % recovery values were found to be within the limits, which showed that the method was accurate. The LOD and LOQ were calculated using statistical methods. The % RSD values were less than 2. The developed method was successfully applied for determination of Pazopanib hydrochloride in pharmaceutical dosage form. The results obtained are in good agreement with those obtained by using the standard method.

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Spectrophotometric Determination of Alendronate Sodium by using Sodium-1,2-Naphthoquinone-4-Sulphonate

International Journal of Pharmaceutical Sciences and Nanotechnology ◽

10.37285/ijpsn.2011.4.4.7 ◽

2012 ◽

Vol 4 (4) ◽

pp. 1563-1568

Author(s):

Sagar Suman Panda ◽

Ravi Kumar B V V ◽

D Patanaik

Keyword(s):

Spectrophotometric Method ◽

Absorption Maximum ◽

Dosage Form ◽

Dosage Forms ◽

Alendronate Sodium ◽

Colored Complex ◽

Pharmaceutical Dosage Forms ◽

Recovery Study ◽

Assay Conditions

A simple, precise and accurate spectrophotometric method was developed for analysis of the osteoporesis drug alendronate sodium (ALS). The method is based on reaction of the drug with sodium-1,2-naphthoquinone-4-sulphonate (NQS) in presence of alkali to form a brown colored complex giving absorption maximum at 525 nm. The drug obeyed Beer’s law in the range of 5-70 µg/ml with a correlation coefficient of 0.999. The LOD and LOQ values are 1.7 µg/ml and 5.0 µg/ml, respectively. The average recoveries for recovery study were found to be in the range of 99.37%-100.46%. The R.S.D. values for intraday and inter-day precision were found to be 0.48 and 0.62, respectively. The optimized assay conditions were applied successfully for determination of ALS in pharmaceutical dosage forms. No interference was observed from the excipients present in the dosage form. The method is statistically validated as per the ICH requirements.

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Determination of galantamine hydrobromide in bulk drug and pharmaceutical dosage form by spectrofluorimetry

Journal of Pharmacy And Bioallied Sciences ◽

10.4103/0975-7406.120079 ◽

2013 ◽

Vol 5 (4) ◽

pp. 314 ◽

Cited By ~ 5

Author(s):

AmitV Patel ◽

VishalJ Patel ◽

AvaniV Patel ◽

JayantB Dave ◽

ChhaganbhaiN Patel

Keyword(s):

Dosage Form ◽

Pharmaceutical Dosage Form ◽

Bulk Drug

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NEW VALIDATED RP-HPLC METHOD FOR THE DETERMINATION OF RIZATRIPTAN BENZOATE IN BULK AND PHARMACEUTICAL DOSAGE FORM

INDIAN DRUGS ◽

10.53879/id.51.12.10213 ◽

2014 ◽

Vol 51 (12) ◽

pp. 32-36

Author(s):

T. Vishalakhi ◽

◽

S. K Kumar ◽

K Sujana ◽

P Rani

Keyword(s):

Mobile Phase ◽

Dosage Form ◽

Hplc Method ◽

Detector Response ◽

Mobile Phase Flow Rate ◽

Pharmaceutical Dosage Form ◽

Rizatriptan Benzoate ◽

Rp Hplc ◽

Bulk Drug

A simple validated RP HPLC method for the estimation of rizatriptan benzoate in pharmaceutical dosage form and bulk was developed for routine analysis. This method was developed by selecting Agilent TC C18 (250 x 4.6 mm, 5 μ) column as stationary phase and acrylonibrile:water (45:55), pH adjusted to 3, as mobile phase. Flow rate of mobile phase was maintained at 4: 1 mL/min at ambient temperature throughout the experiment. Quantification was achieved with ultraviolet (DAD) detection at 220 nm. The retention time obtained for rizatriptan was 2.8 min. The detector response was linear in the concentration range of 2-25μg/mL. This method was validated and shown to be specific, sensitive, precise, linear, accurate, rugged and robust. Hence, this method can be applied for routine quality control of rizatriptan benzoate in dosage forms as well as in bulk drug.

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