Spectrophotometric Determination of Alendronate Sodium by using Sodium-1,2-Naphthoquinone-4-Sulphonate

A simple, precise and accurate spectrophotometric method was developed for analysis of the osteoporesis drug alendronate sodium (ALS). The method is based on reaction of the drug with sodium-1,2-naphthoquinone-4-sulphonate (NQS) in presence of alkali to form a brown colored complex giving absorption maximum at 525 nm. The drug obeyed Beer’s law in the range of 5-70 µg/ml with a correlation coefficient of 0.999. The LOD and LOQ values are 1.7 µg/ml and 5.0 µg/ml, respectively. The average recoveries for recovery study were found to be in the range of 99.37%-100.46%. The R.S.D. values for intraday and inter-day precision were found to be 0.48 and 0.62, respectively. The optimized assay conditions were applied successfully for determination of ALS in pharmaceutical dosage forms. No interference was observed from the excipients present in the dosage form. The method is statistically validated as per the ICH requirements.

Download Full-text

Spectrophotometric analysis of Azithromycin and its pharmaceutical dosage forms: Comparison between Spectrophotometry and HPLC

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v12i2.21981 ◽

2015 ◽

Vol 12 (2) ◽

pp. 171-179 ◽

Cited By ~ 3

Author(s):

Nahid Sharmin ◽

Nazia Sultana Shanta ◽

Sitesh C Bachar

Keyword(s):

Statistical Analysis ◽

Spectrophotometric Method ◽

Dosage Form ◽

Dosage Forms ◽

Spectrophotometric Analysis ◽

Pharmaceutical Dosage Form ◽

Pharmaceutical Dosage Forms ◽

Ich Guidelines ◽

Good Accordance

A simple, reliable, precise and sensitive UV-spectrophotometric method was developed and validated for the estimation of azithromycin in pharmaceutical dosage form and compared with official USP 2010 method. The proposed method utilizes the oxidation of azithromycin with potassium permanganate to liberate formaldehyde. This formaldehyde reacts with acetone-ammonium reagent and produces yellow colored chromogen 3,5-diacetyl-2,6-dihydrolutidine. The colored solution exhibited a maximum absorption at 412 nm which can be detected with UVspectrophotometer. The method was found linear over the concentration range 80% to 120% of the working concentration (R2=0.999). The intra- and inter-day RSD (n = 6) was ? 2.0%. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. The proposed method was successfully applied for determination of azithromycin and the results have been compared with HPLC and thus enabling the utility of this new method for routine analysis azithromycin in pharmaceutical dosage forms DOI: http://dx.doi.org/10.3329/dujps.v12i2.21981 Dhaka Univ. J. Pharm. Sci. 12(2): 171-179, 2013 (December)

Download Full-text

SIMULTANEOUS ESTIMATION AND VALIDATION OF RAMIPRIL AND TELMISARTAN BY UV SPECTROPHOTOMETRY

INDIAN DRUGS ◽

10.53879/id.51.09.10156 ◽

2014 ◽

Vol 51 (09) ◽

pp. 31-35

Author(s):

R Rambabu ◽

◽

S Vidyadhara ◽

J Subbarao

Keyword(s):

Spectrophotometric Method ◽

Correlation Coefficients ◽

Simultaneous Equation ◽

Dosage Forms ◽

Simultaneous Estimation ◽

Uv Spectrophotometry ◽

Intermediate Precision ◽

Pharmaceutical Dosage Forms ◽

Sensitive Spectrophotometric Method

A simple and sensitive spectrophotometric method for the determination of ramipril and telmisartan in pharmaceutical dosage forms has been developed. The absorption maxima were found at 220nm for ramipril and 297nm for telmisartan using 0.1N NaOH as solvent. Beer’s law was obeyed for both the drugs in the concentration range of 2-10μg/ml with correlation coefficients 0.999 for both ramipril and telmisartan. The limits of detection for ramipril and telmisartan were found to be 0.142 and 0.405μg/mL respectively and the limits of quantitation were 0.43 and 1.22μg/mL. Accuracy of the method was verified by performing recovery studies using simultaneous equation method and found to be 98.33 to 99.54%w/w for ramipril and 99.36 to 99.82 %w/w for telmisartan. %RSD of repeatability and intermediate precision studies were found to be <2 for both the drugs. Ruggedness of the method was checked by changing analyst worked and instrument used. In both the cases, the %RSD was found to be less than 2.

Download Full-text

DEVELOPMENT AND VALIDATION OF ULTRAVIOLET-SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF FEBUXOSTAT FOR CONDUCTING IN-VITRO QUALITY CONTROL TESTS IN BULK AND PHARMACEUTICAL DOSAGE FORMS

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i9.25550 ◽

2018 ◽

Vol 11 (9) ◽

pp. 115

Author(s):

Jaspreet Kaur ◽

Daljit Kaur ◽

Sukhmeet Singh

Keyword(s):

Spectrophotometric Method ◽

Limit Of Detection ◽

Pharmaceutical Formulations ◽

Dosage Forms ◽

Pharmaceutical Dosage Forms ◽

Relative Standard ◽

Limit Of Quantitation ◽

Development And Validation

Objective: A simple, accurate, and selective ultraviolet-spectrophotometric method has been developed for the estimation of febuxostat in the bulk and pharmaceutical dosage forms.Method: The method was developed and validated according to International Conference on Harmonization (ICH Q2 R1) guidelines. The developed method was validated statistically with respect to linearity, range, precision, accuracy, ruggedness, limit of detection (LOD), limit of quantitation (LOQ), and recovery. Specificity of the method was demonstrated by applying different stressed conditions to drug samples such as acid hydrolysis, alkaline hydrolysis, oxidative, photolytic, and thermal degradation.Results: The study was conducted using phosphate buffer pH 6.8 and λmax was found to be 312 nm. Standard plot having a concentration range of 1–10 μg/ml showed a good linear relationship with R2=0.999. The LOD and LOQ were found to be 0.118 μg/ml and 0.595 μg/ml, respectively. Recovery and percentage relative standard deviations were found to be 100.157±0.332% and <2%, respectively.Conclusion: Proposed method was successfully applicable to the pharmaceutical formulations containing febuxostat. Thus, the developed method is found to be simple, sensitive, accurate, precise, reproducible, and economical for the determination of febuxostat in pharmaceutical dosage forms.

Download Full-text

VISIBLE SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION OF IMATINIB IN BULK AND FORMULATION

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2020v12i2.37491 ◽

2020 ◽

pp. 63-67

Author(s):

SMITA KUMBHAR ◽

VINOD MATOLE ◽

YOGESH THORAT ◽

ANITA SHEGAONKAR ◽

AVINASH HOSMANI

Keyword(s):

Spectrophotometric Method ◽

Method Development ◽

Pharmaceutical Formulations ◽

Uv Spectrum ◽

Colored Complex ◽

Pharmaceutical Dosage Forms ◽

Highly Sensitive ◽

Method Development And Validation ◽

Development And Validation

Objective: A new, simple, sensitive, precise and reproducible UV visible spectrophotometric method was developed for the determination of Imatinib in pharmaceutical formulations with alizarin. Methods: The method is based on formation of yellow-colored complex. The UV spectrum of Imatinib in methanol showed λ max at 431 nm. Beer’s law is valid in the concentration range of 10-70 μg/ml. This method was validated for linearity, accuracy, precision, ruggedness and robustness. Results: The method has demonstrated excellent linearity over the range of 10-70 μg/ml with regression equation y =0.013x-0.017 and regression correlation coefficient r2= 0.997. Moreover, the method was found to be highly sensitive with LOD (4.3μg/ml) and LOQ (13.07μg/ml). Conclusion: Based on results the proposed method can be successfully applied for the assay of Imatinib in various pharmaceutical dosage forms.

Download Full-text

Derivative spectrophotometric method for simultaneous determination of clindamycin phosphate and tretinoin in pharmaceutical dosage forms

DARU Journal of Pharmaceutical Sciences ◽

10.1186/2008-2231-21-29 ◽

2013 ◽

Vol 21 (1) ◽

Cited By ~ 5

Author(s):

Maliheh Barazandeh Tehrani ◽

Melika Namadchian ◽

Sedigheh Fadaye Vatan ◽

Effat Souri

Keyword(s):

Simultaneous Determination ◽

Spectrophotometric Method ◽

Dosage Forms ◽

Clindamycin Phosphate ◽

Pharmaceutical Dosage Forms

Download Full-text

Area Under the Curve Spectrophotometric Method for Determination of Irbesatran in Pharmaceutical Formulation

IRA-International Journal of Applied Sciences (ISSN 2455-4499) ◽

10.21013/jas.v7.n2.p5 ◽

2017 ◽

Vol 7 (2) ◽

pp. 95

Author(s):

S. Alexandar ◽

A. Santhanam ◽

C. Sandhu ◽

C. Sanjaykanth ◽

S. Sandhya ◽

...

Keyword(s):

Spectrophotometric Method ◽

Area Under Curve ◽

Dosage Form ◽

Area Under The Curve ◽

Pharmaceutical Dosage Form ◽

Pharmaceutical Dosage Forms ◽

Routine Work ◽

Accuracy Study ◽

Bulk Drug

<em>This study presents new spectrophotometric method for the determination of Irbesartan. So far, no Area under Curve Spectrophotometric method has been reported for the estimation of Irbesartan from pharmaceutical dosage form. This paper deals with validation and development of a method by Area Under Curve for the assay of Irbesartan from pharmaceutical dosage forms. The principle for AUC curve method is “the area under two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The area selected between 203 to 211 nm for determination of Irbesartan. The drug follows Beer-Lambert’s law over the concentration range of 5-25 μg/ml for Irbesartan. In accuracy study the % recovery of Irbesartan in bulk drug samples was ranged 96.45-93.84%, which indicates that the method was accurate. Validation of the proposed method was carried out for its accuracy, precision, and specificity according to ICH guidelines. The proposed methods can be successfully applied in routine work for the determination of Tigecycline in its pharmaceutical dosage form.</em>

Download Full-text

Spectrophotometric Determination of Zidovudine in Pharmaceutical Dosage Forms

E-Journal of Chemistry ◽

10.1155/2012/757916 ◽

2012 ◽

Vol 9 (1) ◽

pp. 89-92

Author(s):

J. Sudhakar Reddy ◽

MD. S. Maqsood Ahmed ◽

I. E. Chakravarthy ◽

K. Prabhavathi

Keyword(s):

Statistical Analysis ◽

Dosage Form ◽

Dosage Forms ◽

Ion Pair ◽

Reagent Blank ◽

Pharmaceutical Dosage Forms ◽

Extractable Complex ◽

Bulk Drug ◽

Tablet Dosage

A simple, sensitive and economical spectrophotometric method has been developed for the determination of zidovudine in commercial dosage forms. The method was based on the formation of chloroform extractable complex of zidovudine with wool fast blue. The absorbance of the extractable ion pair complex is measured at the wavelength of maximum absorbance 590 nm against the reagent blank treated similarly. Statistical analysis proves that the proposed methods are reproducible and selective for the estimation of zidovudine in bulk drug and in its tablet dosage form.

Download Full-text