scholarly journals Spectrophotometric Estimation of Amoxicillin Trihydrate in Bulk and Pharmaceutical Dosage Form

2008 ◽  
Vol 5 (s2) ◽  
pp. 1114-1116 ◽  
Author(s):  
K. Prakash ◽  
P. Narayana Raju ◽  
K. Shanta Kumari ◽  
M. Lakshmi Narasu
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Phosphate Buffer ◽  
Dosage Form ◽  
Molar Absorptivity ◽  
Pharmaceutical Dosage Form ◽  
Amoxicillin Trihydrate ◽  
Bulk Drug ◽  
Tablet Dosage

New simple, sensitive, rapid, reproducible and economical spectrophotometric method have been developed for the determination of amoxicillin trihydrate in pharmaceutical bulk and tablet dosage form using citro phosphate buffer pH 7.2. The system obeys Lambert-Beer's law at 231 nm in the concentration range 2.5-50 μg/m. Molar absorptivity, correlation coefficient and Sandell's sensitivity values were found to be 1.0020 x 104mol-1cm,-10.9996 and 0.03906 μg cm-2respectively. The proposed methods have been successfully applied to the analysis of the bulk drug and its tablet dosage form. The methods have been statistically evaluated and were found to be precise and accurate

scholarly journals UV Spectrophotometric Estimation of Levoceterizine Dihydrochloride in Bulk and Dosage Form

2010 ◽  
Vol 7 (s1) ◽  
pp. S414-S418
Author(s):  
P. Mamatha ◽  
P. V. Anantha Lakshmi ◽  
P. L. Prasunamba
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Ultra Violet ◽  
Molar Absorptivity ◽  
Distilled Water ◽  
Maximum Absorbance ◽  
Bulk Drug ◽  
Tablet Dosage

A new simple, rapid, accurate, sensitive and precise spectrophotometric method in ultra violet region has been developed for the determination of levoceterizine dihydrochloride (LCTZ) in bulk drug and tablet dosage form. Levoceterizine dihydrochloride exhibited maximum absorbance at 232 nm with apparent molar absorptivity of 1.5104×104in double distilled water. Beer's law was found to be obeyed in the concentration range of 4-20 µg mL-1. Correlation coefficient was found to be 0.9998. Results of the analysis were validated statistically and by recovery studies. The proposed method is useful for the routine estimation of LCTZ in bulk and tablet dosage form.

scholarly journals Development DEVELOPMENT AND VALIDATION OF NOVEL ULTRAVIOLET SPECTROPHOTOMETRIC METHOD FOR QUANTITATIVE ESTIMATION OF DALFAMPRIDINE IN BULK AND IN PHARMACEUTICAL FORMULATION

2019 ◽  
pp. 275-279
Author(s):  
VAIBHAV S KHODKE ◽  
GAME MD
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Quantitative Estimation ◽  
Spectroscopic Method ◽  
Quality Criteria ◽  
Pharmaceutical Dosage Form ◽  
Development And Validation ◽  
Tablet Dosage ◽  
Good Agreement

Objective: The objective of the present study is to develop ultraviolet (UV)-spectroscopic method using pure drug and tablet dosage form that consistently produces a drug with a minimal variation that adheres to quality criteria of purity, identity, and potency. Methods: UV-spectrophotometric method has been developed using a solvent composed of methanol:water (30:70) as a diluent to determine the dalfampridine (DFP) content in bulk and pharmaceutical dosage form at predetermined λmax of 262 nm. Results: It was proved linear in the range of 02–12 μg/ml and exhibited a good correlation coefficient (r2 = 0.9915) and excellent mean recovery (0.004136347%). This method was successfully applied to the determination of DFP content of marketed tablet Dalstep 10 mg (Sun Pharmaceutical Pvt. Ltd.,) from India; the results were in good agreement with the label claims. Conclusion: The method proved to be simple, accurate, precise, specific, robust, and less time consuming and can be applied for the determination of DFP in bulk and marketed formulation.

scholarly journals Area Under the Curve Spectrophotometric Method for Determination of Irbesatran in Pharmaceutical Formulation

2017 ◽  
Vol 7 (2) ◽  
pp. 95
Author(s):  
S. Alexandar ◽  
A. Santhanam ◽  
C. Sandhu ◽  
C. Sanjaykanth ◽  
S. Sandhya ◽  
...  
Keyword(s):  
Spectrophotometric Method ◽  
Area Under Curve ◽  
Dosage Form ◽  
Area Under The Curve ◽  
Pharmaceutical Dosage Form ◽  
Pharmaceutical Dosage Forms ◽  
Routine Work ◽  
Accuracy Study ◽  
Bulk Drug

<em>This study presents new spectrophotometric method for the determination of Irbesartan. So far, no Area under Curve Spectrophotometric method has been reported for the estimation of Irbesartan from pharmaceutical dosage form. This paper deals with validation and development of a method by Area Under Curve for the assay of Irbesartan from pharmaceutical dosage forms. The principle for AUC curve method is “the area under two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The area selected between 203 to 211 nm for determination of Irbesartan. The drug follows Beer-Lambert’s law over the concentration range of 5-25 μg/ml for Irbesartan. In accuracy study the % recovery of Irbesartan in bulk drug samples was ranged 96.45-93.84%, which indicates that the method was accurate. Validation of the proposed method was carried out for its accuracy, precision, and specificity according to ICH guidelines. The proposed methods can be successfully applied in routine work for the determination of Tigecycline in its pharmaceutical dosage form.</em>

scholarly journals Spectrophotometric Method Development and Validation for Simultaneous Estimation of Nebivolol hydrochloride and Valsartan in Bulk and Combined Pharmaceutical Dosage Form in Release Media

2019 ◽  
Vol 11 (06) ◽  
Author(s):  
Chandani Makvana ◽  
Satyajit Sahoo
Keyword(s):  
Spectrophotometric Method ◽  
Phosphate Buffer ◽  
Dosage Form ◽  
Method Development ◽  
Limit Of Detection ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Limit Of Quantitation ◽  
Nebivolol Hydrochloride ◽  
Tablet Dosage

A simple, rapid, precise, accurate and sensitive spectrophotometric method has been developed for the simultaneous estimation and validation of Nebivolol Hydrochloride (NEB) and Valsartan (VAL) in pure and combined tablet dosage forms. Pure drug samples of NEB and VAL were dissolved in 67 mM Phosphate buffer pH 6.8 with 0.5% sodium dodecyl sulphate (SDS) and found to have absorbance maxima at 280 nm for NEB and 250 nm for VAL, respectively. The linearity lies between 10-70μg/ml for NEB and 10-60μg/ml for VAL in this method. The correlation coefficient (r2) was found to be 0.9965 for NEB and 0.9960 for VAL. The % recoveries obtained were 95.65%-109.85% for NEB and 97.42%-101.43% for VAL. The % RSD found 0.271%-1.490% for intraday and 0.334%-1.917% for interday for NEB and 0.188%-0.944% for intraday and 0.392%-1.197% for interday for VAL. The limit of detection and limit of quantitation for NEB were found to be 4.608μg/ml and 13.965μg/ml respectively and the limit of detection and limit of quantitation for VAL were found to be 4.348μg/ml and 13.178μg/ml respectively. Simultaneous calibration of both drugs in 67 mM Phosphate buffer pH 6.8 with 0.5% SDS shows that λmax of one drug does not interfere on the λmax of other drug. Recovery study was performed to confirm the accuracy of the method. The results of analysis have been validated statistically by recovery studies as per International Conference on Harmonization guidelines. The method showed good reproducibility and recovery with % RSD Lessthan 2. Hence, this proposed method was found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of NEB and VAL in pure and combined tablet dosage form.

scholarly journals New Simple Spectrophotometric Method for the Simultaneous Estimation of Paracetamol and Flupirtine Maleate in Pure and Pharmaceutical Dosage Form

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
P. Giriraj ◽  
T. Sivakkumar
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equation ◽  
Equation Method ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Precision Study ◽  
Tablet Dosage ◽  
Concentration Levels

A new, simple, precise, accurate, reproducible, and efficient Vierordt’s method or simultaneous equation method was developed and validated for simultaneous estimation of paracetamol and flupirtine maleate in pure and pharmaceutical dosage form. The method was based on the measurement of absorbance at two wavelengths 245 nm and 344.5 nm, λmax of paracetamol and flupiritine maleate in 0.1 N HCl correspondingly. Calibration curves of paracetamol and flupiritine maleate were found to be linear in the concentration ranges of 5–15 μg/mL and 1.53–4.61 μg/mL, respectively, with their correlation coefficient values (R2) 0.999. LOD and LOQ were 185.90 ng/mL and 563.38 ng/mL for paracetamol and 78.89 ng/mL and 239.06 ng/mL for flupiritine maleate. In the precision study, the % RSD value was found within limits (RSD<2%). The percentage recovery at various concentration levels varied from 99.18 to 100.02% for paracetamol and 98.47 to 100.09% for flupiritine maleate confirming that the projected method is accurate. It could be concluded from the results obtained in the present investigation that this method for simultaneous estimation of paracetamol and flupirtine maleate in pure and tablet dosage form is simple, accurate, precise, and economical. The proposed method can be applied successfully for the simultaneous estimation of paracetamol and flupiritine maleate in pure and pharmaceutical dosage form.

scholarly journals UV Spectrophotometric Method for the Estimation of Tadalafil in Bulk and Tablet Dosage form

2010 ◽  
Vol 7 (3) ◽  
pp. 833-836 ◽  
Author(s):  
Mohammad Yunoos ◽  
D.Gowri Sankar ◽  
B.Pragati Kumar ◽  
Shahul Hameed
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Dosage Forms ◽  
Form Solution ◽  
Solid Dosage ◽  
Percentage Recovery ◽  
Accuracy And Precision ◽  
Tablet Dosage

A simple, sensitive, precise and highly accurate UV spectrophotometric method has been developed for the determination of tadalafil in bulk and tablet dosage form. Solution of tadalafil in methanol shows maximum absorbance at 284 nm. Beer’s law was obeyed in the concentration range of 2-20 mcg mL-1with 1.65x104mol-1cm-1, the slope, intercept, correlation coefficient, detection and quantitation limits were also calculated. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablets dosage forms. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The method was validated by determining its sensitivity, accuracy and precision which proves suitability of the developed method for the routine estimation of tadalafil in bulk and solid dosage form.

scholarly journals UV Spectrophotometric Method for the Estimation of Valacyclovir HCl in Tablet Dosage Form

2009 ◽  
Vol 6 (3) ◽  
pp. 814-818 ◽  
Author(s):  
M. Ganesh ◽  
C. V. Narasimharao ◽  
A. Saravana Kumar ◽  
K. Kamalakannan ◽  
M. Vinoba ◽  
...  
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Dosage Forms ◽  
Form Solution ◽  
Solid Dosage ◽  
Percentage Recovery ◽  
Accuracy And Precision ◽  
Tablet Dosage

A simple, sensitive, highly accurate UV spectrophotometric method has been developed for the determination of valacyclovir in bulk and tablet dosage form. Solution of valacyclovir in 0.1N HCl shows maximum absorbance at 255 nm. Beer’s law was obeyed in the concentration range of 5-25 mcg mL-1with 1.0910x104mol-1cm-1, the slope, intercept, correlation coefficient, detection and quantitation limits were also calculated. The proposed method has been applied successfully for the analysis of the drug in pure and in its tablets dosage forms. Result of percentage recovery and placebo interference shows that the method was not affected by the presence of common excipients. The percentages assay of valacyclovir HCl in tablet was 99.82%. The method was validated by determining its sensitivity, accuracy and precision which proves suitability of the developed method for the routine estimation of valacyclovir in bulk and solid dosage form.

scholarly journals A VALIDATED SPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF VILAZODONE HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORM

2016 ◽  
Vol 9 (1) ◽  
pp. 132 ◽  
Author(s):  
Nita Yadav ◽  
Anju Goyal
Keyword(s):  
Spectrophotometric Method ◽  
Dosage Form ◽  
Research Work ◽  
Order Derivative ◽  
Pharmaceutical Dosage Form ◽  
Label Claim ◽  
The Mean ◽  
Tablet Dosage ◽  
Accuracy Data

Objective: In the present research work three simple, accurate, precise methods of the UV-visible spectrophotometric method was developed and validated for the estimation of Vilazodone HCl in bulk and tablet dosage.Methods: Three methods were used for estimation of Vilazodone HCl using methanol. Method A involves zero order spectroscopy at absorption maximum of 241 nm; Method B involves first order derivative at 246.5 nm and Method C involves second-order derivative at 243.5 nm. The developed methods were validated according to ICH guidelines.Results: The developed methods were found to be linear in the concentration range of 1-5 µg/ml. The mean percentage label claim of Vilazodone HCl was within the acceptable range. The accuracy data showed % recovery and % RSD within the range.Conclusion: The developed methods were found to be accurate and precise. The % RSD values were within limits. These methods can be used for the routine analysis of Vilazodone HCl in bulk and tablet dosage form.

scholarly journals Spectrophotometric Method for the Determination of Nebivolol Hydrochloride in Bulk and Pharmaceutical Formulations

2010 ◽  
Vol 7 (2) ◽  
pp. 445-448 ◽  
Author(s):  
A. Lakshmana Rao ◽  
K. R. Rajeswari ◽  
G. G. Sankar
Keyword(s):  
Correlation Coefficient ◽  
Antihypertensive Drug ◽  
Spectrophotometric Method ◽  
Pharmaceutical Formulations ◽  
Molar Absorptivity ◽  
Dosage Forms ◽  
Percentage Recovery ◽  
Nebivolol Hydrochloride ◽  
Tablet Dosage

A simple, sensitive, highly accurate spectrophotometric method in UV region has been developed for the determination of nebivolol hydrochloride in bulk and pharmaceutical formulations. Nebivolol hydrochloride is an antihypertensive drug, which shows maximum absorbance at 281 nm with apparent molar absorptivity of 5.37208 × 103mol-1cm-1. Beer’s law was obeyed in the concentration range of 4-60 μg/mL. The slope, intercept and correlation coefficient were also calculated. The proposed method has been successfully used for the analysis of the drug in pure and its tablet dosage forms. Results of percentage recovery shows that the method was not affected by the presence of common excipients. The percentage assay of nebivolol hydrochloride was 98.75 and 99.02 respectively. The method was validated by sensitivity and precision which proves suitability of proposed method for the routine estimation of nebivolol in bulk and pharmaceutical formulations.

IR QUANTIFICATION OF MODAFINIL IN BULK AND ORAL DOSAGE FORM

INDIAN DRUGS ◽  
2018 ◽  
Vol 55 (10) ◽  
pp. 63-65
Author(s):  
A. M Sekar ◽  
◽  
S Anbazhagan ◽  
R Agalya ◽  
T. Asha ◽  
...  
Keyword(s):  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Limit Of Detection ◽  
Oral Dosage ◽  
Limit Of Quantification ◽  
Oral Dosage Form ◽  
Precision And Accuracy ◽  
Bulk Drug ◽  
Tablet Dosage

Simple and sensitive infrared spectrophotometric method has been developed for the estimation of modafinil in tablet dosage form and the Beer’s Law concentration range was found to be 1.0mg to 2.5mg. The correlation coefficient for the method was found to be 0.999 and the developed method was analyzed for specificity, limit of detection (LOD), limit of quantification (LOQ), linearity of response, precision and accuracy; thus the proposed method could be adopted for routine analysis of bulk drug and its formulation.

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