scholarly journals Uji Mutu dan Keefektifan Metarhizium anisopliae Isolat Kalimantan Tengah Terhadap Oryctes rhinoceros (Coleoptera: Scarabaeidae) [Quality Test and Effectiveness of Metarhizium anisopliae Isolates From Central Kalimantan Against Oryctes rhinocerus]

Buletin Palma ◽  
2018 ◽  
Vol 19 (2) ◽  
pp. 79
Author(s):  
Siswanto Nls ◽  
I M Trisawa
Keyword(s):  
Biological Control ◽  
Metarhizium Anisopliae ◽  
Biological Control Agent ◽  
Control Agent ◽  
Quality Test ◽  
Central Kalimantan ◽  
Oryctes Rhinoceros ◽  
Quality Grade ◽  
Medicinal Crops

<p><em>Oryctes rhinoceros</em> (Coleoptera: Scarabaeidae) is  an importan pest on coconut trees. Control of <em>O. rhinoceros</em> can be done in various ways, such as by physical, mechanical, biological, and chemical. The fungus <em>Metarhizium anisopliae</em> is a biological agent that can suppress the development of the <em>O. rhinoceros</em> . Effectiveness of <em>M. anisopliae</em> fungus is largely determined by the quality/grade conidia of the fungus consist of its density, viability and patogenicity against the target pest. For that, studies have been conducted to determine the quality and effectiveness biological control of <em>M. anisopliae</em> isolates Kalteng (Central Kalimantan) against <em>O. rhinoceros</em>. Tests conducted in the Entomology Laboratory of Research Institute for Spices and Medicinal Crops  (Balittro), and around the home screen in Balittro, Bogor.  The fungus <em>M. anisopliae</em> isolates originating from Central Kalimantan (Kalteng), while the larvae of <em>O. rhinoceros</em> from Yogyakarta. The results showed that the fungus conidia <em>M. anisopliae</em> isolates Kalteng has a density of over 10<sup>8</sup>, while the viability above 90%. Pathogenicity to larvae of <em>O. Rhinoceros</em> &gt; 50%. This character indicates a good quality of these fungi as biological control agent. The fungus of <em>M. anisopliae </em>isolates Kalteng at concentrations of 10<sup>6</sup> and 10<sup>8</sup> can killed up to 90% third instar O. rhinoceros larvae  in field conditions. So both these concentrations can be used in the control of <em>O. rhinoceros</em> in the field. </p><p><strong><br /></strong></p><p><strong>ABSTRAK</strong><em> </em></p><p>Hama <em>Oryctes rhinoceros</em> (Coleoptera: Scarabaeidae) merupakan hama penting tanaman kelapa . Pengendalian <em>O. rhinoceros </em>dapat dilakukan dengan berbagai cara yaitu secara fisik, mekanik, hayati, dan kimiawi.  Cendawan  <em>Metarhizium anisopliae</em> merupakan agens hayati yang dapat menekan perkembangan hama <em> O. rhinoceros</em>. Efektivitas cendawan <em>M. anisopliae</em> sangat ditentukan oleh kualitas/mutu konidia cendawan tersebut yang meliputi kerapatan, viabilitas  dan patogenitasnya terhadap hama sasaran. Untuk itu telah dilakukan penelitian untuk mengetahui mutu dan keefektifan agens pengendali hayati  <em>M. anisopliae </em>isolat Kalimantan Tengah terhadap <em>O. rhinoceros. </em>Pengujian dilakukan di Laboratorium Hama Balai Penelitian Tanaman Rempah dan Obat (Balittro) dan di sekitar rumah kasa di Balittro, Bogor.  Isolat cendawan <em>M. anisopliae </em>yang digunakan berasal dari  Kalteng, sedangkan larva <em>O. rhinoceros </em>berasal dari Yogyakarta. Hasil penelitian menunjukkan bahwa Cendawan <em>M. anisopliae </em>isolat Kalteng memiliki kerapatan konidia di atas 10<sup>8</sup>, sedangkan viabilitasnya di atas 90%.  Patogenisitas terhadap larva <em>O. rhinoceros </em>&gt; 50%.  Karakter ini menunjukkan mutu yang baik dari cendawan tersebut sebagai agens pengendali hayati. Cendawan <em>M. anisopliae </em>isolat Kalteng pada konsentrasi 10<sup>6</sup> dan 10<sup>8</sup> dapat mematikan 90%  larva <em>O. rhinoceros </em>instar 3 pada kondisi lapangan. Sehingga kedua konsentrasi tersebut dapat digunakan dalam mengendalikan <em>O. rhinoceros </em>di lapangan.</p>

scholarly journals Evaluation of cellulose substrates treated with Metarhizium anisopliae (Metschnikoff) Sorokin as a biological control agent against the termite Microcerotermes diversus Silvestri (Isoptera: Termitidae)

2011 ◽  
Vol 43 (2) ◽  
pp. 269
Author(s):  
Behzad Habibpour ◽  
Amir Cheraghi ◽  
Mohammad Saeed Mossadegh
Keyword(s):  
Biological Control ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Biological Control Agent ◽  
Control Agent ◽  
Cellulose Substrates ◽  
Virulent Strains ◽  
Sustainable Protection ◽  
Chemical Pesticides

This article is the first report on the promising effect of an entomopathogenic fungus, <em>Metarhizium anisopliae</em> (Metschnikoff) Sorokin to control populations of <em>Microcerotermes diversus </em>Silvestri. Biological control is an alternative to the long-term usage of chemical pesticides.<em> M. anisopliae</em>, the causal agent of green muscardine disease of insects, is an important fungus in biological control of insect pests. Bait systems can eliminate entire colonies of subterranean termites. Baiting reduces adverse environmental impacts caused by organochlorine and organophosphate pesticides in the control of termites and creates sustainable protection of buildings against their invasion. Treated-sawdust bait was applied by two methods: a) combination of treated sawdust and untreated filter paper, and b) combination of treated sawdust and untreated sawdust. When combinations of treated sawdust and untreated sawdust were used, LC50 and LC90 were 8.4&times;106 and 3.9&times;107 (spore/ml), respectively. With the use of improved bait formula and more virulent strains, we hope to achieve better control of termite colonies and enable pathogens to become a useful element in the Integrated Pest Management system.

scholarly journals Pathogenicity of Beauveria bassiana Vuill in compost media for Oryctes rhinoceros L. oil palm pest control

2021 ◽  
Vol 4 (1) ◽  
pp. 1
Author(s):  
Rina Novianti ◽  
Hafiz Fauzana ◽  
Rusli Rustam
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Pest Control ◽  
Oil Palm ◽  
Biological Control Agent ◽  
Early Death ◽  
Breeding Site ◽  
Control Agent ◽  
Oryctes Rhinoceros ◽  
Plant Pest

The Oryctes rhinoceros pest is an important pest of palm oil plant.  Pest problems occur because  Oil Palm Empty Fruit Bunches (OPEFB) is added for soil fertility to create a breeding site for larvae O. rhinoceros. Generally, OPEFB will receive the plants more quickly when composted, and pest control is carried out in the compost. Biological control is more recommended because it is environmentally friendly, Therefore compost is added with biological control agent O. rhinoceros namely B. bassiana. This study is aimed to obtain the best conidia density of Beauveria bassiana in compost in controlling larvae O. rhinoceros. The research was carried out at the Plant Pest Laboratory and Experimental Garden, Faculty of Agriculture, Riau of University. The study was carried out from February to November 2020. The experiment on the conidia density of B. bassiana fungi in compost media against larvae O. rhinoceros, using a Completely Randomized Design (CRD), with 6 treatments 4 replications obtained 24 experimental units, while the treatments were 0 g.l-1, 15 g.l-1, 30 g.l-1, 45 g.l-1, 60 g.l-1 and 75 g.l-1. The results of the research revealed that  OPEFB compost + sawdust containing the fungus B. bassiana 75 g.l-1 (83,2 x108 kon/ml) had the best ability to control larvae O. rhinoceros with a total larvae mortality of 87% which caused early death of 54 hours after application, LT50 of 213 hours after application, and LC50 of 3,3% or the equivalent of 33 g.l-1 at 14 days after application.

Pathogenicity of Metarhizium anisopliae and Beauveria bassiana against Phyllophaga crinita and Anomala flavipennis (Coleoptera: Scarabaeidae)

2005 ◽  
Vol 40 (1) ◽  
pp. 67-73 ◽  
Author(s):  
L. A. Rodríguez-del-Bosque ◽  
F. Silvestre ◽  
V. M. Hernández ◽  
H. Quiroz ◽  
J. E. Throne
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Metarhizium Anisopliae ◽  
Biological Control Agent ◽  
Challenge Test ◽  
Control Agent ◽  
The Other ◽  
White Grub ◽  
Test Protocol ◽  
Lethal Time

Five isolates of Beauveria bassiana (Balsamo) Vuillemin and 3 isolates of Metarhizium anisopliae (Metschnikoff) Sorokin were tested against third-instar larvae of Phyllophaga crinita (Burmeister) and Anomala flavipennis Burmeister under laboratory conditions using the “maximum challenge test” protocol. The M. anisopliae strains were more virulent than the B. bassiana strains, with the isolates MAGL3N and MAGL4N of M. anisopliae causing the highest mortality in both white grub species. Regardless of scarab species, mortality caused by MAGL3N was &gt;63% after 4 days and &gt;96% after 10 days. The other strain of M. anisopliae, MAGC2N, also caused high mortality in A. flavipennis, but at a slower rate than MAGL3N. Median lethal time (LT50) for MAGL3N was 2.9 days for P. crinita and 3.0 d for A. flavipennis. The LT50 for MAGL4N was 5.3 d for P. crinita and 7.6 d for A. flavipennis while the LT50 for MAGC2N was 4.4 d for A. flavipennis. Metarhizium anisopliae is a potential biological control agent for P. crinita and A. flavipennis and should be further investigated for possible development.

scholarly journals Effects of Metarhizium anisopliae on host choice of the bee-parasitic mite Varroa destructor

Acarologia ◽  
2018 ◽  
Vol 58 (2) ◽  
pp. 287-295
Author(s):  
Lara Reinbacher ◽  
María Celeste Fernández-Ferrari ◽  
Sergio Angeli ◽  
Peter Schausberger
Keyword(s):  
Biological Control ◽  
Metarhizium Anisopliae ◽  
Varroa Destructor ◽  
Biological Control Agent ◽  
Fungal Spores ◽  
Host Choice ◽  
Control Agent ◽  
Host Selection Behavior ◽  
Varroa Mites ◽  
Parasitic Mite

The ectoparasitic mite Varroa destructor poses one of the biggest threats to the western honeybee Apis mellifera. Possibilities to control the mites are limited; therefore, it is important to assess the performance of novel alternatives like the use of biological control agents. The goal of our work was to evaluate the effects of the entomopathogenic fungus Metarhizium anisopliae var. anisopliae BIPESCO 5, which is a potential biological control agent against the bee parasitic mite V. destructor, on the behavior of the parasite. In detail, we investigated whether the presence of fungal spores on the surface of host nurse bees, A. mellifera, affects the host choice behavior of adult female mites. We conducted two behavioral assays to monitor the behavior of the mites towards adult bees inoculated with a solution of fungal spores. Both choice and no-choice experiment revealed that M. anisopliae has a significant repellent influence on V. destructor‘s host selection behavior. The mites preferred, and stayed longer on, nurse bees free from fungal spores over bees carrying fungal spores. Our study provides the first evidence of sub-lethal behavioral effects of a fungal biocontrol agent against Varroa mites.

Pathogenicity of an Indigenous Strain of the Entomopathogenic Fungus Metarhizium anisopliae (Hypocreales: Clavicipitaceae) (MET-GRA4 Strain) as a Potential Biological Control Agent Against the Red Palm Weevil (Coleoptera: Dryophthoridae)

2019 ◽  
Author(s):  
I Ishak ◽  
L C Ng ◽  
M Haris-Hussain ◽  
J Jalinas ◽  
A B Idris ◽  
...  
Keyword(s):  
Biological Control ◽  
Dna Sequences ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Biological Control Agent ◽  
Control Agent ◽  
Red Palm Weevil ◽  
Palm Weevil ◽  
Lethal Time

Abstract Metarhizium anisopliae Metchnikoff (Hypocreales: Clavicipitaceae) is a fungal pathogen that causes disease in various insect pests, and it can be exploited and developed as a biological control agent to combat the red palm weevil, Rhynchophorus ferrugineus Olivier (Coleoptera: Dryophthoridae). The study on indigenous isolates is crucial especially for development of bioinsecticides in the future. The M. anisopliae strain called MET-GRA4 was tested for pathogenicity against adult red palm weevil and treated in vitro with different spore viabilities. The isolates exhibited pathogenicity with 100% mortality 21 d postinfection. The median lethal time (LT50) for 85% viable spores was 8.6 d, while 39% viable spores had an LT50 value of 21.37 d, with 92 and 16.6% mycosis, respectively. The species MET-GRA4 strain was molecularly characterized using ITS1 and ITS4 from pure culture (Isolate A), mass-produced spores (Isolate B), and infected red palm weevil cadavers (Isolate C). The DNA sequences obtained matched M. anisopliae sequences, with 99% similarity. This new isolate of M. anisopliae has potential as a targeted bioinsecticide for management of red palm weevil.

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