scholarly journals Pengaruh Media terhadap Produksi Prodigiosin Isolat Bakteri Entomopatogen Serratia marcescens Asal Wereng Batang Cokelat

2016 ◽  
Vol 10 (2) ◽  
pp. 77
Author(s):  
Ifa Manzila ◽  
Tri P. Priyatno ◽  
Rahminovita Herlis ◽  
Iman Rusmana ◽  
I Made Samudra ◽  
...  
Keyword(s):  
Thin Layer ◽  
Serratia Marcescens ◽  
Silica Gel ◽  
Luria Bertani ◽  
Red Pigment ◽  
Purity Level ◽  
Red Color ◽  
The Family ◽  
The Media ◽  
Layer Chromatography

<p>Prodigiosin, the red pigment produced<br />by the bacterium Serratia marcescens, is a secondary<br />metabolite of the family tripyrrole that has been widely used<br />as an antibiotic in the multifunction treatment of<br />antibacterial as well as antifungal. This study was aimed to<br />study the effect of Luria-Bertani (LB) broth and nutrient<br />broth (NB) media suplemented with several concentrations<br />of FeSO4 and CaCO3 on the production and characteristic of<br />prodigiosin derived from S. marcescens. The study was<br />arranged in a completely randomized factorial design with<br />four replications. The LB and NB media were supplemented<br />with 0, 2.5, 5, and 10 mM CaCO3 and 0, 0.25, 0.5, and 1 mM<br />FeSO4. Results showed a red pigment produced by S.<br />marcescens when cultured on both LB and NB media. Redlike<br />pigmentation was varied when supplemented with<br />different concentration of Fe2+ and Ca2+. The higher the<br />concentration of Fe2+, the more intense the red color,<br />conversely, the higher the concentration of Ca2+, the lighter<br />the red color. The interaction was found between the media<br />and concentrations of CaCO3 and FeSO4 on the production<br />of prodigiosin. The highest prodigiosin production was<br />obtained on NB media supplemented with FeSO4.<br />Meanwhile, the addition of CaCO3 did not affect the<br />prodigiosin production. An addition of 1 mM FeSO4 to LB<br />and NB media produced crude prodigiosin of 486.0 mg/ml<br />and 489.0 mg/ml, respectively. Based on purification by<br />column chromatography using silica gel, the prodigiosin<br />production on LB and NB media was 378 mg/ml and 450<br />mg/ml, with the purity level of 77.8% and 92%, respectively.<br />Detection of prodigiosin by thin-layer chromatography using<br />silica gel showed the red pigment had Rf value of 0.83 and<br />bioautography assay showed there was an antibacterial<br />activity against Xanthomanas oryzae pv. oryzae.</p>

New Records of Graphidaceous Lichens from Western Ghats, India

2014 ◽  
Vol 37 (4) ◽  
pp. 477-481
Author(s):  
H. Biju ◽  
R. Bagool ◽  
Sanjeeva Nayaka
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Western Ghats ◽  
New Records ◽  
The Family ◽  
Chemical Studies ◽  
Solvent Systems ◽  
Layer Chromatography ◽  
Lichen Flora

Six lichen species belonging to the family Graphidaceae namely Graphis malacodes Nyl., Graphis proserpens Vain., Hemithecium aphaneomicrosporum Makhija & Adawadkar, Hemithecium lamii (Redgr.) V. Tewari & Upreti, Pallidogramme chlorocarpoides (Nyl.) Staiger & al. and Phaeographis divaricoides Räsänen are described as new additions to the lichen flora of Western Ghats. Chemical studies were carried out by thin layer chromatography using Merk F254 precoated silica gel aluminium plates and B.D.A. solvent systems.

scholarly journals Umbelliprenin from Ferula persica Roots Inhibits the Red Pigment Production in Serratia marcescens

2004 ◽  
Vol 59 (7-8) ◽  
pp. 506-508 ◽  
Author(s):  
Mehrdad Iranshahi ◽  
Ahmad R. Shahverdi ◽  
Roohollah Mirjani ◽  
Gholamreza Amin ◽  
Abbas Shafiee
Keyword(s):  
Antibacterial Activity ◽  
Thin Layer ◽  
Petroleum Ether ◽  
Serratia Marcescens ◽  
Preparative Thin Layer Chromatography ◽  
Chloroform Extract ◽  
Pigment Production ◽  
Red Pigment ◽  
Active Fraction ◽  
Layer Chromatography

AbstractThe chloroform extract of Ferula persica var. persica roots was found to inhibit red pigment production of Serratia marcescens. A bioguided fractionation study by preparative thin layer chromatography (PTLC) detected a fraction (Rf = 0.71, petroleum ether/EtOAc, 2:1 v/v), which was effective on depigmentation of Serratia marcescens. Using conventional spectroscopy methods, the active fraction was identified as umbelliprenin. Neither the chloroform extract nor the isolated umbelliprenin fraction showed any antibacterial activity against the test strain at a certain concentration. In contrast, they exhibited depigmentation zones on culture plates.

DEVELOPMENT OF HPTLC METHOD FOR THE ESTIMATION OF ONDANSETRON AND RANITIDINE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (12) ◽  
pp. 42-48
Author(s):  
P. J. Patel ◽  
◽  
D. A Shah ◽  
F. A. Mehta ◽  
U. K. Chhalotiya
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Dosage Form ◽  
Solvent System ◽  
Rf Values ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent system was methanol. The Rf values were observed to be 0.5 ± 0.02, and 0.3 ± 0.02 for OND and RAN, respectively. The separated spots were densitometrically analyzed in absorbance mode at 299 nm. This method was linear in the range of 25-300 ng/band for OND and 50-600 ng/band for RAN. The limits of detection for OND and RAN were found to be 3.47 and 1.83 ng/band, respectively. The limits of quantification for OND and RAN were found to be 10.53 and 5.55 ng/band, respectively. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of OND and RAN in combined dosage form.

scholarly journals Normal and reversed phase thin-layer chromatography of new 16, 17-secoestrone derivatives

2003 ◽  
Vol 68 (1) ◽  
pp. 57-64 ◽  
Author(s):  
Marijana Acanski ◽  
Suzana Jovanovic-Santa ◽  
Lidija Jevric
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Reversed Phase ◽  
Retention Behavior ◽  
Mobile Phases ◽  
Layer Chromatography

The retention behavior and separation ability of a series of new 16,17-secoestrone derivatives has been studied on silica gel, alumina and C-18 silica gel layers with non-aqueous and aqueous-organic mobile phases. The retention behavior and separation ability are discussed in terms of the nature of the solute, eluent and stationary phase.

Sign in / Sign up

Export Citation Format

Share Document