scholarly journals Identifikasi Marka Polimorfik untuk Pemuliaan Padi Toleran Defisiensi Fosfor

2016 ◽  
Vol 4 (2) ◽  
pp. 51
Author(s):  
Joko Prasetiyono ◽  
Hajrial Aswidinoor ◽  
Sugiono Moeljopawiro ◽  
Didy Sopandie ◽  
Masdiar Bustamam
Keyword(s):  
Molecular Biology ◽  
Ssr Markers ◽  
Silver Staining ◽  
Staining Method ◽  
Polyacrylamide Gel ◽  
Background Selection ◽  
Silver Staining Method ◽  
Rice Genotypes ◽  
Biology Laboratory ◽  
Selection Markers

<p>Information on polymorphisms<br />among rice parents are very important in rice<br />breeding for tolerance to phosphorus defficiency. A study<br />was conducted at the Molecular Biology Laboratory,<br />Indonesian Center Agricultural Biotechnology and Genetic<br />Resources (ICABIOGRAD) from October 2006 to July 2007 to<br />identify polymorphism markers from 6 rice genotypes. The<br />rice genotypes, i.e., Dodokan, Situ Bagendit, Batur, Kasalath,<br />NIL-C443, dan K36-5-1-1 were analyzed for polymorphisms<br />using 496 SSR markers, which cover the rice genomes.<br />Seven of the 496 markers were used as foreground and<br />recombinant selection markers, and the rests (489 markers)<br />were used as background selection markers. PCR amplifications<br />were separated on a 5% polyacrylamide gel and<br />colored by the silver staining method. Three different markers<br />among the seven foreground and recombinant selection<br />markers were selected from each crossing, which are<br />tightly linked with Pup1 gene and have a distance less than 5<br />cM. These markers are Dodokan vs Kasalath (RM277, SSR3,<br />RM519), Dodokan vs NIL-C443 (RM277, SSR3, RM519),<br />Dodokan vs K36-5-1-1 (RM277, SSR3, RM519), Situ Bagendit<br />vs Kasalath (RM28102, SSR3, RM519), Situ Bagendit vs NILC443<br />(RM28102, SSR3, RM519), Situ Bagendit vs K36-5-1-1<br />(RM511, SSR3, RM519), Batur vs Kasalath (RM277, RM1261,<br />RM519), Batur vs NIL-C443 (RM277, RM1261, RM519), and<br />Batur vs K36-5-1-1 (RM28102, SSR3). Variations in background<br />selection primers were found in each chromosome<br />and in each parent combinations. Primers on chromosome<br />4, 5, and 12 showed the lowest polymorphisms; more<br />primers are needed for these chromosomes.</p>

An acidic silver staining method for synaptonemal complexes under light microscopy

1984 ◽  
Vol 133 (1) ◽  
pp. 99-102 ◽  
Author(s):  
José Fernandez-Piqueras ◽  
Carlos Sentis Castaño ◽  
E. Rojo Garcia ◽  
A. Rodriguez Campos
Keyword(s):  
Light Microscopy ◽  
Silver Staining ◽  
Staining Method ◽  
Synaptonemal Complexes ◽  
Silver Staining Method

Nucleolar organizer activity at meiosis in wheat–rye hybrid plants

1986 ◽  
Vol 28 (2) ◽  
pp. 227-234 ◽  
Author(s):  
N. Cuñado ◽  
M. C. Cermeño ◽  
J. Orellana
Keyword(s):  
Silver Staining ◽  
Staining Method ◽  
Nucleolar Organizer ◽  
The Other ◽  
Nucleolar Organizer Regions ◽  
Binucleate Cells ◽  
Hybrid Plants ◽  
Nucleolar Organizer Activity ◽  
Silver Staining Method ◽  
Organizer Activity

Nucleoli and nucleolar organizer regions (NORs) have been studied by a silver staining method in all meiotic stages of wheat–rye hybrid plants. The maximum number of nucleoli per cell scored at meiotic prophase and tapetum binucleate cells indicates that only the NORs of 1B, 6B, and 5D wheat chromosomes are active, whereas that of chromosome IR (SAT) of rye is inactive. However, at diakinesis, metaphase and anaphase meiotic stages only chromosomes 1B and 6B show Ag-NOR as was reported previously in somatic metaphase. The absence of Ag-NOR on chromosome 5D has been imputed to its low nucleolar organizer activity, not detectable by silver staining, because of the small number of rDNA clusters it carries. On the other hand, the meiotic behaviour of chromosomes 1B and 6B has been studied at metaphase I and anaphase I, using the Ag-NORs as cytological markers.Key words: nucleolar organizer, Ag-NOR, meiosis, wheat–rye hybrids.

scholarly journals Combined beta-galactosidase and immunogold/silver staining for immunohistochemistry and DNA in situ hybridization.

1990 ◽  
Vol 38 (3) ◽  
pp. 325-329 ◽  
Author(s):  
W van den Brink ◽  
C van der Loos ◽  
H Volkers ◽  
R Lauwen ◽  
F van den Berg ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Silver Staining ◽  
Staining Method ◽  
Double Staining ◽  
Reaction Products ◽  
Silver Enhancement ◽  
Silver Staining Method ◽  
Double Staining Method ◽  
Beta Galactosidase

A combination of beta-galactosidase enzyme and the immunogold/silver staining method was studied for evaluation of double-staining experiments. Applications are shown for immunohistochemical double staining using two monoclonal antibodies and for combined immunohistochemistry and DNA in situ hybridization in one tissue section. The following advantages for the present double-staining method were evaluated: superior sensitivity of the immunogold/silver staining method for at least one epitope, which also allows detection of biotinylated DNA probes. The structure of the indolyl precipitate after revelation of beta-galactosidase activity did not show a concealing effect during a sequential double-staining method, as compared with the visualization of peroxidase with diaminobenzidine. These factors, and the sharply contrasting colored reaction products of beta-galactosidase (blue-green) and the immunogold/silver staining method including silver enhancement (brown-black), allow clear distinction of mixed-stained cell constituents.

scholarly journals Changes in the number and size of nucleoli of Chara vulgaris L. antheridial filament cells during the period preceding light-induced re-initiation of cell divisions following a mitodepressive effect of darkness

2014 ◽  
Vol 68 (4) ◽  
pp. 251-254
Author(s):  
Maria Kwiatkowska ◽  
Ewa Papiernik
Keyword(s):  
Cell Cycle ◽  
Developmental Stage ◽  
Mitotic Activity ◽  
Silver Staining ◽  
Staining Method ◽  
Chara Vulgaris ◽  
Cell Divisions ◽  
A Cell ◽  
Silver Staining Method

The changes in number and size of nucleoli of <em>Chara vulgaris</em> antheridial filament cells were monitored with the use of Howell and Black's silver staining method. After a 3-day mitodepressive treatment with darkness the cells were exposed to light which reactivated mitotic activity after 18-20 hours. Eight-celled antheridial filaments were observed. In the period preceding light-induced re-initiation of mitoses a gradual reconstruction of the number and size of nucleoli characteristic of control, as well as their total area per nucleus appeared. The obtained results indicate that one of the important conditions for a cell to be able to divide is accumulation of nucleolus components characteristic of a given developmental stage and this controls nucleologenesis of the subsequent cell cycle.

scholarly journals A Silver Staining Method for Single-cell Gel Assay

2001 ◽  
Vol 49 (9) ◽  
pp. 1183-1186 ◽  
Author(s):  
Silvina B. Nadin ◽  
Laura M. Vargas–Roig ◽  
Daniel R. Ciocca
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Single Cell ◽  
Silver Staining ◽  
Staining Method ◽  
Human Lymphocytes ◽  
Fluorescence Staining ◽  
High Quality ◽  
Dna Damage And Repair ◽  
Silver Staining Method

The single-cell gel assay (comet assay) is a very useful microelectrophoretic technique for evaluation of DNA damage and repair in individual cells. Usually, the comets are visualized and evaluated with fluorescent DNA stains. This staining requires specific equipment (e.g., a high-quality fluorescence microscope), the slides must be analyzed immediately, and they cannot be stored for long periods of time. Here we describe, using human lymphocytes, some modifications of the silver staining for comets that significantly increase the sensitivity/reproducibility of the assay. This silver staining was compared with fluorescence staining and commercial silver stains. (J Histochem Cytochem 49:1183–1186, 2001)

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