scholarly journals Study the role of auxins and sytoknins on in vitro propagation of Spilanthes acmella (L.) Murr

2014 ◽  
Vol 11 (3) ◽  
pp. 1367-1372
Author(s):  
Baghdad Science Journal
Keyword(s):  
Growth Regulators ◽  
In Vitro Propagation ◽  
Peat Moss ◽  
Naphthalene Acetic Acid ◽  
In Vitro Micropropagation ◽  
Spilanthes Acmella ◽  
Half Strength ◽  
The Mean

The technique of plant tissue culture has been used to In vitro micropropagation of Spilanthes acmella (L.) Murr. It is an ornamental and medicinal plant not cultivated in Iraq. Seeds were sterilized and cultuared on full strength Murashige and Skoog medium(1962)(MS). Naphthalene acetic acid (NAA), 6- furfuryl aminopurine (Kin.) growth regulators were used at the Initiation stage.The combination between IAA and Kin. was used in multiplication stage. IAA was used for rooting the shoots. Results showed that 1.5% sodium hypochlorite for 15 min was very effective for disinfecting and survival. Nodes exhibited relatively highest response as compared with apical meristems and leaflets culture. Supplying the culture medium with 1 mg/L. Kin. was effective for lateral shoot induction. The mean number of shoots obtained from nodes were 4.12 with a mean length 2.70 cm. Adding Kin. at 0.5, 1.0 or 1.5 and IAA at 0.1 mg/L. to the growth medium was effective for multiplication. Mean number of the developed shoots were 13.60, 14.20, 12.00 respectively. The best result of rooting stage was achieved by half- strength MS medium without growth regulators which produced 27.50 roots/ plantlet with mean length 3.90 cm. Results of acclimatization stage showed that addition of 1:1 peat moss and loamy soil gave the highest rate of survival(100)% after 4 weeks of acclimatization. This study showed the ability of in vitro propagation of Spilanthes acmella (L.) Murr.

scholarly journals n vitro micro propagation of Spilanthes acmella (L.) Murr

2014 ◽  
Vol 8 (1) ◽  
pp. 55-60
Author(s):  
Bushra M. Jaber Alwash ◽  
Ansaam Z. Jassim
Keyword(s):  
In Vitro Propagation ◽  
Indole Acetic Acid ◽  
Ms Medium ◽  
Indole Butyric Acid ◽  
Micro Propagation ◽  
Spilanthes Acmella ◽  
Half Strength ◽  
The Mean ◽  
Initiation Stage

This study was aimed to In vitro propagation of Spilanthes acmella L. Murr. It is a medicinal plant not cultivated in Iraq. Seeds were sterilized and cultured on MS medium. Indole acetic acid IAA, Benzyladenin BA growth regulators’ were used at the initiation stage. The combination between IAA and BA was used in multiplication stage. Indole butyric acid IBA was used for rooting the shoots. Results showed that 1.5% sodium hypochlorite for 15 min was very effective for disinfecting and survival. A node exhibited relatively highest response as compared with apical meristems and leaflets culture. Supplying the culture medium with 1mg/l. BA was effective for lateral shoot induction. The mean number of shoots obtained from nodes were 7.43 with a mean length 0.9 cm. Adding BA at 0.5, 1.0 or 1.5 and IAA at 0.1 mg/l. to the growth medium was effective for multiplication. Mean number of the developed shoots were 12.00, 10, 84, 10.00 respectively. Adding 0.1, 0.5, 1.0 mg/l IBA to the half strength MS medium was very effective in root formation which produced 45.0, 42.5, 40.0 roots respectively with mean length of 3.25, 3.80, 3.80 cm respectively. Results of acclimatization stage showed that addition of 1:1 Patmos and loamy soil gave the highest rate of survival 100% after 4 weeks of acclimatization. This study showed the ability of in vitro propagation of Spilanthes acmella (L.) Murr

scholarly journals In vitro propagation of Althaea officinalis : the role of plant growth regulators in morphogenesis

2017 ◽  
Vol 98 (3) ◽  
pp. 167-173
Author(s):  
A. Mujib ◽  
Tanu Pipal ◽  
Muzamil Ali ◽  
Dipti Tonk ◽  
Nadia Zafar ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Althaea Officinalis

scholarly journals In vitro: Influence of Various Concentrations of Plant Growth Regulators (BAP & NAA) and Sucrose on Regeneration of Chenopodium quinoa Willd. Plant

2020 ◽  
pp. 34-43
Author(s):  
Fayza R. Al Gethami ◽  
Hameda El Sayed Ahmed El Sayed
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Chenopodium Quinoa ◽  
Naphthalene Acetic Acid ◽  
Cotyledonary Nodes ◽  
Half Strength ◽  
The Media

In vitro: regeneration of Chenopodium quinoa Willd. was achieved from cotyledonary nodes explants. In this study, used 6-Benzylaminopurine (BAP) and α-Naphthalene Acetic Acid (NAA) of plant growth regulators with different concentrations individually as well as in combination and used different concentrations of sugar (sucrose) with different concentrations. For was rooting, used half strength (½MS), full-strength MS and ½ MS supplemented with 0.2 mg/l of NAA. The results mentioned, explant responding (%) to multiplication was about 73% for all BAP treatments compared with control and average numbers of shoot increased with increased BAP concentration except 5 mg/l of BAP. The highest explant responding (%) was in media supplemented BAP without NAA compared other treatments noted that the media with combination of BAP and NAA gives formation of callus in bases of the plantlets. Also, the result inducted the combinations between (BAP–NAA) was highly significantly (P≤ 0.001) and less effective on number of shoots where the highest number of shoot was 3.40 in media with 3 mg/l BAP compared other treatments. The highest of explant responding 93.33% was in media supplement with 10 g/l sucrose and (10 g/l sucrose + 3 mg/l BAP), but sucrose level for good greening and developed shoots (4 shoots) was in medium supplement with 10 g/l sucrose. The shoots rooted well on half-strength MS medium with 60% percentage of root. The rooted shoots were acclimatized and transferred to green house to follow their development.

scholarly journals Establishment and In Vitro Propagation of a Putative Variant of Periwinkle

2000 ◽  
Vol 5 (1) ◽  
pp. 15
Author(s):  
A. S. AI-Wasel
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Murashige And Skoog Medium ◽  
Half Strength ◽  
Bud Breaking

Shoot multiplication of a putative variant of Catharanthus roseus (L.) G. Don, was achieved in vitro using shoot tips and nodal segments as explants. The addition of growth regulators to establishment medium stimulated bud breaking and shoot elongation. The maximum shoot multiplication (15.1 shoots/microshoot) and the longest shoots (7.0 cm) occurred on Murashige and Skoog medium (MS) containing 1.0 mg L-1 of N6-Benzyladenine (BA) and a- Naphthalene acetic acid (NAA). All microshoots formed roots and normal root morphology occurred on half strength MS salt supplied with 0.5 mg L-1 NAA or Indole-B-Butyric acid (IBA). Rooted microshoots (95 %) were successfully transferred to soil.

scholarly journals COMPARISON OF TWO METHODS FOR IN VITRO PROPAGATION OF RAUWOLFIA SERPENTINA FROM NODAL EXPLANTS

2007 ◽  
Vol 44 (07) ◽  
pp. 514-519 ◽  
Author(s):  
Ved Prakash Pandey ◽  
Jose Kudakasseril ◽  
Elizabeth Cherian ◽  
George Patani ◽  
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
In Vitro Multiplication ◽  
Rauwolfia Serpentina ◽  
In Vitro Micropropagation

Two different methods of in vitro multiplication of Rauwolfia serpentina from nodal explants were compared viz. multiplication via callus morphogenesis and that via shoot proliferation from axillary buds. The second method was found to be far better. The optimum shoot proliferation occurred on Murashige and Skoog (MS) medium supplemented with 1 mg/L naphthalene acetic acid (NAA) and 2 mg/L of benzyl aminopurine (BAP). The best rooting of shoots occurred on MS medium containing 4% sucrose and 1 mg/L of NAA. Solid and liquid MS media were found to be similar in supporting shoot proliferation. The plants produced were successfully hardened and established in soil. An easy, reliable and reproducible protocol was developed for in vitro micropropagation of Rauwolfia serpentina from nodal explants.

scholarly journals Clonal Micropropagation of representatives of the genus Dactylorhiza Neck. ex Nevski

2021 ◽  
Vol 4 (46) ◽  
pp. 17-17
Author(s):  
Alexander Saakian ◽  
◽  
Keyword(s):  
Survival Rate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Culture Media ◽  
Ms Medium ◽  
Organic Additives ◽  
Clonal Micropropagation ◽  
Half Strength

Abstract The aim of this study is to develop and improve methods of in vitro propagation of representatives of Dactylorhiza: D.baltica , D. fuchsii. For the study, we used protocorms obtained by the asymbiotic germination of seed during 90 days. It has been established that half-strength of Murashige and Skoog (1962) medium (½ MS) supplemented with 1-2 mg/l 6-Benzylaminopurine(6-BAP), potato puree (20g/l), and charcoal (1g/l) effectively influenced the development of protocorms, and seedlings formation in the studied species. The result of the study showed that the survival rate of protocorms was high in all experimental culture media, but in D. fuchsii it was better at a concentration 2mg/l of 6-BAP (95.4%), while in D. baltica it was high at 1mg/l (87.0%). The highest percentage of multiple protocorms (68%) and the formation of new secondary protocorms in D. fuchsii (5,5±0,3 units) were observed on a culture medium containing 2 mg/l 6-BAP. The highest percent of rooting of D. fuchsii protosoms (78%) and length of roots (0.9cm) observed in ½ MS medium without growth regulators. During the development of D. baltica protosoms, the culture medium of ½ MS containing 1 mg/l 6-BAP had the best effect on the number of roots (1.8±0.1root/protosom), while the medium supplemented with 2mg/l of 6-BAP contributed to the formation of a larger number of new secondary protocorms (3,2±0,1protocorm/unit). During the subsequent cultivation of protosoms of D. baltica on a culture medium containing 1 mg/l it was observed an increase in the height of shoots (4,8±0,3 см), and the length of roots (2,2±0,1 см), wherein the number of newly formed protocorms was higher by 30% on the medium supplemented with 2 mg/l 6-BAP. Keywords: DACTYLORHIZA BALTICA, DACTYLORHIZA FUCHSII, IN VITRO, PROTOCORMS, ORGANIC ADDITIVES

scholarly journals In vitro propagation of banana

1970 ◽  
Vol 34 (2) ◽  
pp. 269-278
Author(s):  
M Rezaul Karim ◽  
MA Malek ◽  
Sajia Rahman ◽  
M Al-Amin ◽  
M Ruhul Amin
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Room Temperature ◽  
Ms Medium ◽  
Musa Sp ◽  
In Vitro Technique ◽  
Half Strength ◽  
The Mean ◽  
Basal Media

An in vitro technique for plant regeneration using meristem-derived plantlets of banana cv. BARI-l (Musa sp.) has been developed. Highest number of shoot regeneration was noticed on basal media supplemented with 7.5 mgL-1 BAP + 0.5 mgL-1 NAA at 30 days after inoculation (DAI). The mean number of shoots significantly reduced when the concentrations of BAP and NAA in the medium was high. Regenerated shoots were rooted on half strength MS medium containing 0.5 mgL-1 IAA + 0.5 mgL-1 IBA at 30 DAI. In vitro raised plantlets were transferred to poly bags containing ground soil and cowdung mixture (1:1) for acclimatization and hardening in room temperature (28-30°C) and the established plantlets are ready for planting in the field. Key Words: In vitro propagation; banana; Musa sp.DOI: 10.3329/bjar.v34i2.5799Bangladesh J. Agril. Res. 34(2): 269-278, June 2009

scholarly journals Germination, in vitro Propagation and Acclimatization in Lavandula Angustifolia

2018 ◽  
Vol 75 (2) ◽  
pp. 105
Author(s):  
Ileana MICLEA ◽  
Raluca CHIFOR
Keyword(s):  
Growth Regulators ◽  
In Vitro Propagation ◽  
Growth Parameters ◽  
In Vitro Germination ◽  
Lavandula Angustifolia ◽  
Plant Propagation ◽  
Breaking Dormancy ◽  
Half Strength ◽  
Plant Acclimatization

This research focused on finding the best method for seed in vitro germination in Lavandula angustifolia and optimizing the medium for plant propagation. Seeds were sterilized and subjected to various treatments to break dormancy, then placed on half-strength MS (1/2MS) or distilled H2O + phytagel. Germination percentages were assessed and plantlets propagated on MS without growth regulators or with zeatin (0.5, 1, 2 mg/l), 1 mg/l BA + 0.5 mg/l IBA, 2 mg/l BA + 1 mg/l IBA or 3 mg/l BA + 1.5 mg/l IBA. After 8 weeks growth parameters were recorded and plants were acclimatized. Immersion in 20 mg/100 ml GA3 solution for 24 hours at 4°C was the most effective in breaking dormancy. Stratification at 4°C for 8 weeks and soaking in a solution of 0.5% H2O2 at 23°C for 24 hours also proved beneficial but to a smaller degree. Half-strength MS was the best germination medium. Shoot development was the highest in MS supplemented with zeatin (2 or 1 mg/l) while roots formed only in the control. Callus induction percentage was best in the presence of 3 mg/l BA + 1.5 mg/l IBA but decreasing concentrations increased callus weight. Plant acclimatization was more successful in moss:sand - 1:2 than in vermiculite:perlite:sand - 2:2:1.

Improved in vitro propagation of Rose cultivars against varying concentrations of carbon source, agar and plant growth regulators

2015 ◽  
Vol 22 (2) ◽  
pp. 69-73
Author(s):  
Kumud Saklani ◽  
Hem Pant ◽  
Vinod Bisht ◽  
Arun Singh ◽  
Vijay Rawat
Keyword(s):  
Carbon Source ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Ms Medium ◽  
Half Strength ◽  
High Sucrose

The present study was conducted to improve upon the micropropagation protocol of Rose cultivars by modification of the MS medium through variations in sucrose and agar concentrations thereby enhancing the shoot multiplication and rooting efficiency of the nodal explants. High sucrose concentrations and low agar concentrations favoured shoot mutiplication during the in vitro stages. Enhanced multiplcation and growth was observed on sub culturing the mother explants with regenerated shoots on fresh MS medium containing sucrose (3.5%, w/v) and agar (0.6%, w/v), supplemented with lower concentrations cytokinin combination of BAP and KN (2.5 mgl-1+1.5 mgl-1) respectively. Half strength MS medium containing sucrose (2.0%, w/v) and agar (0.3%, w/v) with NAA and BAP (2.0 mgl-1+0.5 mgl-1) in combination was most effective for rooting.

scholarly journals Regeneration and Cormels Production of White Prosperity and Priscilla Gladiolus Varities In Vitro

2009 ◽  
Vol 6 (3) ◽  
pp. 432-441
Author(s):  
Baghdad Science Journal
Keyword(s):  
Shoot Regeneration ◽  
Callus Cultures ◽  
Peat Moss ◽  
Naphthalene Acetic Acid ◽  
Root Number ◽  
Benzyl Adenine ◽  
River Sand ◽  
Callus Initiation

Plant regeneration and cormel production was carried out from callus cultures initiated from White Prosperity and Priscilla Gladiolus Varities. It is aimed to produce plants and cormels in vitro all year round. The study included many experiments, these were the effect of Naphthalene acetic acid (NAA) and Kinetin (Kin) interaction on callus initiation, effect of Benzyl adenine (BA) on shoot regeneration from callus culture, effect of NAA on rooting after 30, 40 and 50 days in culture. The role of the type of agricultural medium (Peat moss or river sand and their mixture on plantlets survival after weaning was studied. Results showed that the interaction between NAA and Kin induced callus on axillary bud explants. Callus was best initiated by using a combination (10.0, 0.5) mg/l for White Prosperity, (0.5, 1.0) and (10.0, 0.5) mgl for Priscilla of NAA and Kin respectively. Regeneration for the two varieties was best occurred when media were supplemented with BA at 1.0 mg/l achieving maximum number of shoots (6.2) and height (4.96 cm.). Highest response for shoot regeneration from callus occurred at a concentration of 0.5 mg/l NAA reached 100% and 83.3% for White Prosperity and Priscilla respectively. An obvious increase in rooting percentage, root number and length over time. Both varieties showed 100% response for cormels formation 50 days after rooting. Plantlets are well established in peat moss.

Sign in / Sign up

Export Citation Format

Share Document