scholarly journals Ion exchange chromatography of organic acids using a chemically bonded hydrophilic anion-exchange column with potassium biphthalate eluent.

1991 ◽  
Vol 40 (1) ◽  
pp. T15-T19 ◽  
Author(s):  
Yoshihito SUZUKI ◽  
Hitoshi KOIZUMI ◽  
Kazue TANI ◽  
Mingyu DING
Keyword(s):  
Ion Exchange ◽  
Organic Acids ◽  
Anion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Anion Exchange Column ◽  
Exchange Column ◽  
Potassium Biphthalate

Isolation and Fast Purification o f Neocarzinostatin by FPLC-Ion Exchange Chromatography

1983 ◽  
Vol 38 (11-12) ◽  
pp. 939-942
Keyword(s):  
Ion Exchange ◽  
Anion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
New Method ◽  
Clinical Grade ◽  
Anion Exchange Column ◽  
Exchange Column ◽  
Culture Filtrates ◽  
Reproducible Method

Neocarzinostatin, a highly toxic antitum or protein containing an essential nonprotein chromophore, can be isolated and purified from culture filtrates of Streptomyces carzinostaticus. Usually a lengthy procedure of up to 60 h is necessary for the isolation, including several chromatographic steps partly under conditions which favour inactivation of the drug by release of chromophore. We describe a new method yielding practically clinical grade Neocarzinostatin from crude extracts in 20 min. This very fast and reproducible method was made possible by using a Mono Q anion exchange column filled with monodisperse gel material which has been recently developed

Quantitation of o- and p-Sulfamoylbenzoic Acids in Commercial Saccharin by High-Performance Liquid Chromatography

1976 ◽  
Vol 59 (2) ◽  
pp. 243-250
Author(s):  
James J Nelson
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ion Exchange ◽  
Sodium Salt ◽  
Mobile Phase ◽  
High Performance ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Anion Exchange Column ◽  
Para Isomer

Abstract Quantitation of o- and p-sulfamoylbenzoic acid residues in saccharin and its sodium salt is achieved by a method comprising methanolic extraction and high-performance ion exchange chromatography. A commercially available anion exchange column was employed with an aqueous buffered (pH 9.2) mobile phase. As little as 80 ppm of the ortho-isomer and 25 ppm of the para-isomer can be accurately determined. The levels of detectability (2 times noise) are estimated as 8 ppm (0.16 μg on column) and 2.5 ppm (0.05 μg on column), respectively. Recoveries from saccharin ranged from 92.7 to 96.5% (ortho) and from 92.2 to 103.3% (para). Recoveries from the sodium salt ranged from 93.1 to 104.4% (ortho) and from 93.5 to 97.8% (para). Of 9 other potential saccharin impurities tested separately, only one was found to interfere slightly in the chromatographic part of the procedure.

Evaluation of the thermal effect on separation selectivity in anion-exchange processes using superheated water ion-exchange chromatography

The Analyst ◽  
2012 ◽  
Vol 137 (13) ◽  
pp. 3154 ◽  
Author(s):  
Masami Shibukawa ◽  
Akihiko Taguchi ◽  
Yusuke Suzuki ◽  
Kazunori Saitoh ◽  
Toshihiko Hiaki ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Thermal Effect ◽  
Anion Exchange ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Superheated Water ◽  
Separation Selectivity ◽  
Exchange Processes

Comparative study of the separation of casein from bovine, ovine and caprine milks using HPLC

1993 ◽  
Vol 60 (4) ◽  
pp. 495-504 ◽  
Author(s):  
Stelios E. Kaminarides ◽  
Emmanuel M. Anifantakis
Keyword(s):  
Ion Exchange ◽  
Comparative Study ◽  
Anion Exchange ◽  
Deae Cellulose ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Strong Anion Exchange ◽  
Milk Casein ◽  
Better Than

SummaryThe separation of cows', sheep's and goats' milk casein components by HPLC on a strong anion-exchange (P.L-SAX 8μ 1000A) column is described. During HLPC, whole caseins of the three kinds of milk behaved differently from conventional separations. The casein components of the three kinds of milk were well resolved under the Chromatographie conditions used. HPLC resolved the κ-caseins better than did ion-exchange chromatography on DEAE cellulose, and was particularly efficient in the case of goats' milk. Goats' and sheep's milks had almost similar Chromatographie profiles but these differed considerably from that of cows' milk. Caseins from the sheep and the goat were also similar in that a shallower NaCl gradient was required for the separation of casein components than for cows' milk.

scholarly journals Selective capture of hexavalent chromium from an anion-exchange column of metal organic resin–alginic acid composite

2016 ◽  
Vol 7 (3) ◽  
pp. 2427-2436 ◽  
Author(s):  
Sofia Rapti ◽  
Anastasia Pournara ◽  
Debajit Sarma ◽  
Ioannis T. Papadas ◽  
Gerasimos S. Armatas ◽  
...  
Keyword(s):  
Ion Exchange ◽  
Anion Exchange ◽  
Hexavalent Chromium ◽  
Alginic Acid ◽  
Anion Exchange Column ◽  
Exchange Column ◽  
Ion Exchange Column ◽  
Metal Organic ◽  
Organic Resin

An ion-exchange column of metal organic resin (MOR-1)–alginic acid (HA) composite shows remarkable efficiency and selectivity for sorption of Cr(vi).

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