scholarly journals Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa)

2020 ◽  
Author(s):  
Eui-Jung Kim ◽  
Sung-Wook Park ◽  
Woo-Jong Hong ◽  
Jeniffer Silva ◽  
Wanqi Liang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Pollen Tube ◽  
Subcellular Localization ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Tip Growth ◽  
Tube Growth ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Polar Tip Growth

Abstract Background: In plants, the key roles played by RopGEF-mediated ROP signaling in diverse processes, including polar tip growth, have been identified. Despite their important roles in reproduction, a comprehensive analysis of RopGEF members has not yet been performed in rice (Oryza sativa). To determine whether RopGEF regulators are involved in rice pollen tube growth, we performed genome-wide analysis of this family in rice.Results: Phylogenomic and meta-expression analysis of eleven RopGEFs in rice showed that four genes were preferentially expressed in mature pollen. These four genes contain the plant-specific Rop nucleotide exchanger (PRONE) domain and possible phosphorylated residues, suggesting a conserved role in polar tip growth with Arabidopsis thaliana. In subcellular localization analysis of the four RopGEFs through tobacco (Nicotiana benthamiana) infiltration, four proteins were predominantly identified in plasma membrane. Moreover, double mutants of RopGEF2/8 exhibited reduced pollen germination, causing partial male sterility. These genes possess unique cis-acting elements in their promoters compared with the other RopGEF genes. Conclusions: In this study, four RopGEF genes were identified as pollen-specific gene in eleven members of rice, and the expression pattern, promoter analysis, and evolutionary relationship of the RopGEF family were studied compared with Arabidopsis. Our study indicated that four RopGEF genes might function during pollen germination in distinct subcellular localization. Our study could provide valuable information on the functional study of RopGEF in rice.

scholarly journals Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa)

2019 ◽  
Author(s):  
Eui-Jung Kim ◽  
Sung-Wook Park ◽  
Woo-Jong Hong ◽  
Jeniffer Silva ◽  
Ki-Hong Jung ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Pollen Tube ◽  
Subcellular Localization ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Tip Growth ◽  
Tube Growth ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Polar Tip Growth

Abstract Background: In plants, the key roles played by RopGEF-mediated ROP signaling in diverse processes, including polar tip growth, have been identified. Despite their important roles in reproduction, a comprehensive analysis of RopGEF members has not yet been performed in rice (Oryza sativa). To determine whether RopGEF regulators are involved in rice pollen tube growth, we performed genome-wide analysis of this family in rice. Results: Phylogenomic and meta-expression analysis of eleven RopGEFs in rice showed that four genes were preferentially expressed in mature pollen. These four genes contain the plant-specific Rop nucleotide exchanger (PRONE) domain and possible phosphorylated residues, suggesting a conserved role in polar tip growth with Arabidopsis. In subcellular localization analysis of the four RopGEFs through tobacco (Nicotiana benthamiana) infiltration was identified in various cellular organelles, indicating their diverse roles during pollen germination in rice. Moreover, these candidate genes possessing unique cis-acting elements in their promoters compared with the other genes. Conclusions: In this study, four RopGEF genes were identified as pollen-specific gene in eleven members of rice, and the expression pattern, promoter analysis, and evolutionary relationship of the RopGEF family were studied compared with Arabidopsis. Our study indicated that four RopGEF genes might function during pollen germination in distinct subcellular localization. Our study could provide valuable information on the functional study of RopGEF in rice.

scholarly journals Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa)

2020 ◽  
Author(s):  
Eui-Jung Kim ◽  
Sung-Wook Park ◽  
Woo-Jong Hong ◽  
Jeniffer Silva ◽  
Wanqi Liang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Pollen Tube ◽  
Subcellular Localization ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Tip Growth ◽  
Tube Growth ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Polar Tip Growth

Abstract Background : In plants, the key roles played by RopGEF-mediated ROP signaling in diverse processes, including polar tip growth, have been identified. Despite their important roles in reproduction, a comprehensive analysis of RopGEF members has not yet been performed in rice ( Oryza sativa ). To determine whether RopGEF regulators are involved in rice pollen tube growth, we performed genome-wide analysis of this family in rice. Results : Phylogenomic and meta-expression analysis of eleven RopGEFs in rice showed that four genes were preferentially expressed in mature pollen. These four genes contain the plant-specific Rop nucleotide exchanger (PRONE) domain and possible phosphorylated residues, suggesting a conserved role in polar tip growth with Arabidopsis thaliana . In subcellular localization analysis of the four RopGEFs through tobacco ( Nicotiana benthamiana ) infiltration, four proteins were predominantly identified in plasma membrane. Moreover, double mutants of RopGEF2/8 exhibited reduced pollen germination, causing partial male sterility. These genes possess unique cis-acting elements in their promoters compared with the other RopGEF genes. Conclusions : In this study, four RopGEF genes were identified as pollen-specific gene in eleven members of rice, and the expression pattern, promoter analysis, and evolutionary relationship of the RopGEF family were studied compared with Arabidopsis. Our study indicated that four RopGEF genes might function during pollen germination in distinct subcellular localization. Our study could provide valuable information on the functional study of RopGEF in rice.

scholarly journals Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa)

2019 ◽  
Author(s):  
Eui-Jung Kim ◽  
Sung-Wook Park ◽  
Woo-Jong Hong ◽  
Jeniffer Silva ◽  
Wanqi Liang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Pollen Tube ◽  
Subcellular Localization ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Tip Growth ◽  
Tube Growth ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Polar Tip Growth

Abstract Background : In plants, the key roles played by RopGEF-mediated ROP signaling in diverse processes, including polar tip growth, have been identified. Despite their important roles in reproduction, a comprehensive analysis of RopGEF members has not yet been performed in rice ( Oryza sativa ). To determine whether RopGEF regulators are involved in rice pollen tube growth, we performed genome-wide analysis of this family in rice. Results : Phylogenomic and meta-expression analysis of eleven RopGEFs in rice showed that four genes were preferentially expressed in mature pollen. These four genes contain the plant-specific Rop nucleotide exchanger (PRONE) domain and possible phosphorylated residues, suggesting a conserved role in polar tip growth with Arabidopsis thaliana . In subcellular localization analysis of the four RopGEFs through tobacco ( Nicotiana benthamiana ) infiltration, four proteins were predominantly identified in plasma membrane. Moreover, double mutants of RopGEF2/8 exhibited reduced pollen germination, causing partial male sterility. These genes possess unique cis-acting elements in their promoters compared with the other RopGEF genes. Conclusions : In this study, four RopGEF genes were identified as pollen-specific gene in eleven members of rice, and the expression pattern, promoter analysis, and evolutionary relationship of the RopGEF family were studied compared with Arabidopsis. Our study indicated that four RopGEF genes might function during pollen germination in distinct subcellular localization. Our study could provide valuable information on the functional study of RopGEF in rice.

scholarly journals Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa)

2020 ◽  
Author(s):  
Eui-Jung Kim ◽  
Sung-Wook Park ◽  
Woo-Jong Hong ◽  
Jeniffer Silva ◽  
Wanqi Liang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Pollen Tube ◽  
Subcellular Localization ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Tip Growth ◽  
Tube Growth ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Polar Tip Growth

Abstract Background : In plants, the key roles played by RopGEF-mediated ROP signaling in diverse processes, including polar tip growth, have been identified. Despite their important roles in reproduction, a comprehensive analysis of RopGEF members has not yet been performed in rice ( Oryza sativa ). To determine whether RopGEF regulators are involved in rice pollen tube growth, we performed genome-wide analysis of this family in rice. Results : Phylogenomic and meta-expression analysis of eleven RopGEFs in rice showed that four genes were preferentially expressed in mature pollen. These four genes contain the plant-specific Rop nucleotide exchanger (PRONE) domain and possible phosphorylated residues, suggesting a conserved role in polar tip growth with Arabidopsis thaliana . In subcellular localization analysis of the four RopGEFs through tobacco ( Nicotiana benthamiana ) infiltration, four proteins were predominantly identified in plasma membrane. Moreover, double mutants of RopGEF2/8 exhibited reduced pollen germination, causing partial male sterility. These genes possess unique cis-acting elements in their promoters compared with the other RopGEF genes. Conclusions : In this study, four RopGEF genes were identified as pollen-specific gene in eleven members of rice, and the expression pattern, promoter analysis, and evolutionary relationship of the RopGEF family were studied compared with Arabidopsis. Our study indicated that four RopGEF genes might function during pollen germination in distinct subcellular localization. Our study could provide valuable information on the functional study of RopGEF in rice.

scholarly journals Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa)

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Eui-Jung Kim ◽  
Sung-Wook Park ◽  
Woo-Jong Hong ◽  
Jeniffer Silva ◽  
Wanqi Liang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Pollen Tube ◽  
Gene Family ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Genome Wide Analysis ◽  
Genome Wide

Attempts to overcome prefertilization incompatibility in interspecific and intergeneric crosses involving Oryza sativa L.

Genome ◽  
1990 ◽  
Vol 33 (3) ◽  
pp. 321-327 ◽  
Author(s):  
L. A. Sitch ◽  
G. O. Romero
Keyword(s):  
Oryza Sativa ◽  
Pollen Tube ◽  
Boric Acid ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Agronomic Traits ◽  
Oryza Sativa L ◽  
Interspecific Cross ◽  
Pollen Tubes ◽  
Tube Growth

The wild relatives of rice are a valuable source of desirable agronomic traits, but prefertilization barriers operate in crosses of Oryza sativa L. with the wild Oryza species and related genera. Pollen germination was normal in crosses with O. brachyantha, O. eichingeri, O. officinalis, and O. ridleyi and slightly inhibited in crosses with Rhynchoryza subulata. Stigmal penetration of O. brachyantha and R. subulata pollen tubes was inhibited, while O. ridleyi pollen tubes showed both incompatible and weakly compatible reactions. Pollen tubes of O. eichingeri, O. officinalis, and O. ridleyi penetrated the stigma, but growth was frequently inhibited in the style or ovary wall, particularly in O. eichingeri crosses. The effect of postpollination application of boric acid, the immunosuppressant ε-aminocaproic acid (EACA), and gibberellic acid (GA3) and of ambient temperature (29 and 35 °C) on germination and pollen tube growth was investigated. Boric acid, EACA, and GA3 inhibited germination of O. brachyantha pollen, and boric acid and EACA inhibited pollen tube growth of O. brachyantha, O. eichingeri, O. ridleyi (35 °C), and R. subulata (29 °C). Pollen tube growth in the control and GA3 treatments was similar. Temperature had no effect on pollen germination and pollen tube growth was stimulated in O. ridleyi crosses at 35 °C only. The nature of incompatibility operating in these crosses is discussed.Key words: incompatibility, pollen tube growth, fertilization, Oryza, interspecific cross, intergeneric cross.

scholarly journals Hyperoside promotes pollen tube growth by regulating the depolymerization effect of actin-depolymerizing factor 1 on microfilaments in okra

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Biying Dong ◽  
Qing Yang ◽  
Zhihua Song ◽  
Lili Niu ◽  
Hongyan Cao ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Pollen Tubes ◽  
Tube Growth ◽  
Actin Depolymerizing Factor ◽  
Specific Mechanism ◽  
Promoting Effect ◽  
New Research

AbstractMature pollen germinates rapidly on the stigma, extending its pollen tube to deliver sperm cells to the ovule for fertilization. The success of this process is an important factor that limits output. The flavonoid content increased significantly during pollen germination and pollen tube growth, which suggests it may play an important role in these processes. However, the specific mechanism of this involvement has been little researched. Our previous research found that hyperoside can prolong the flowering period of Abelmoschus esculentus (okra), but its specific mechanism is still unclear. Therefore, in this study, we focused on the effect of hyperoside in regulating the actin-depolymerizing factor (ADF), which further affects the germination and growth of pollen. We found that hyperoside can prolong the effective pollination period of okra by 2–3-fold and promote the growth of pollen tubes in the style. Then, we used Nicotiana benthamiana cells as a research system and found that hyperoside accelerates the depolymerization of intercellular microfilaments. Hyperoside can promote pollen germination and pollen tube elongation in vitro. Moreover, AeADF1 was identified out of all AeADF genes as being highly expressed in pollen tubes in response to hyperoside. In addition, hyperoside promoted AeADF1-mediated microfilament dissipation according to microfilament severing experiments in vitro. In the pollen tube, the gene expression of AeADF1 was reduced to 1/5 by oligonucleotide transfection. The decrease in the expression level of AeADF1 partially reduced the promoting effect of hyperoside on pollen germination and pollen tube growth. This research provides new research directions for flavonoids in reproductive development.

In Vitro Germination of Eucalyptus Pollen: Response to Variation in Boric Acid and Sucrose

1989 ◽  
Vol 37 (5) ◽  
pp. 429 ◽  
Author(s):  
BM Potts ◽  
JB Marsden-Smedley
Keyword(s):  
Pollen Tube ◽  
Boric Acid ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Sucrose Concentration ◽  
Response Surfaces ◽  
Tube Growth ◽  
Pollen Competition

The effect of boric acid (0-450 ppm) and sucrose (0-40%) on pollen germination and pollen tube growth in Eucalyptus globulus, E. morrisbyi, E. ovata and E. tirnigera was examined in vitro. Over the con- centrations tested, sucrose had by far the largest effect upon both pollen germination and tube lengths. The optimum sucrose concentration for pollen germination (30%) and pollen tube growth (20%) differed markedly with very little (<lo%) germination occurring in the absence of sucrose. The interaction of sucrose and boric acid was significant. However, in general both pollen germination and pollen tube growth were increased by the addition of up to 100 ppm boric acid, but above this level the response plateauxed. The four species differed significantly in their pattern of response to both boric acid and sucrose and the predicted optima derived from analysis of response surfaces differed between species. The predicted sucrose concentration for optimal germination and growth of E. urnigera pollen was consistently less than the other species and in terms of the optimal level of boric acid for pollen tube growth species can be ranked in the order E. globulus > E. ovata > E. morrisbyi = E. urnigera. Pollen germination and tube growth of all four species on a medium comprising 20% sucrose and 200 ppm boric acid would not differ significantly from the observed maximum response of each species and this could suffice as a generalised medium. However, if only percentage germination is to be assessed 30% sucrose would be preferable. It is argued that subtle interspecific differences in optimal in vitro con- ditions for pollen germination and pollen tube growth are likely to reflect differences in pollen physiology which in vivo may have important implications for the success of hybridisation where pollen competition occurs.

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