Potential biological control of the pupal stage of the European grapevine moth Lobesia botrana by the entomopathogenic fungus Beauveria pseudobassiana in the winter season in Chile

Abstract Objective: Lobesia botrana, the European grapevine moth, affects Vitis vinifera L. and other species of economic importance in a number of countries through damage caused by its larvae in berries and associated secondary diseases such as Botrytis cinerea. Control of the moth in urban areas is difficult due to poor chemical management of infested plants in houses. Additionally, in winter, L. botrana is in its pupal stage covered with a cocoon that prevents the penetration of chemical pesticides. For this reason, the objective of this work was to control the pupal stage with a formulation based on the entomopathogenic fungus Beauveria pseudobassiana in urban areas. Results: The present study of the biocontrol activity of the formulated fungus against the infestation of vines with breeding pupae without cocoons showed 100% infection 21 days after inoculation under winter conditions. Finally, the biocontrol activity of the formulated fungus against natural infestations of L. botrana in winter in urban areas reached an efficacy of 51%. This result suggests that the B. pseudobassiana formulation is able to penetrate the cocoon and contributes to the integrated pest management of L. botrana.

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Potential biological control of the pupal stage of the European grapevine moth Lobesia botrana by the entomopathogenic fungus Beauveria pseudobassiana in the winter season in Chile

10.21203/rs.2.11451/v2 ◽

2019 ◽

Author(s):

Fabiola Altimira ◽

Nathalia De La Barra ◽

Patricia Rebufel ◽

Sylvana Soto ◽

Rodrigo Soto ◽

...

Keyword(s):

Entomopathogenic Fungus ◽

Urban Areas ◽

Winter Season ◽

Pupal Stage ◽

Lobesia Botrana ◽

Vitis Vinifera L ◽

European Grapevine Moth ◽

Biocontrol Activity ◽

Grapevine Moth ◽

Chemical Pesticides

Abstract Objective: Lobesia botrana, the European grapevine moth, affects Vitis vinifera L. and other species of economic importance in a number of countries through damage caused by its larvae in berries and associated secondary diseases such as Botrytis cinerea . Control of the moth in urban areas is difficult due to poor chemical management of infested plants in houses. Additionally, in winter, L. botrana is in its pupal stage covered with a cocoon that prevents the penetration of chemical pesticides. For this reason, the objective of this work was to control the pupal stage with a formulation based on the entomopathogenic fungus Beauveria pseudobassiana in urban areas. Results: The strain RGM 1747 was identified as B. pseudobassiana by multilocus sequence analysis . The biocontrol activity of this formulated fungus against the infestation of vines with breeding pupae without cocoons showed 100% infection 21 days after inoculation under winter conditions. Finally, the biocontrol activity of the formulated fungus against natural infestations of L. botrana in winter in urban areas reached an efficacy of 51%. This result suggests that the B. pseudobassiana formulation is able to penetrate the cocoon and contributes to the integrated pest management of L. botrana .

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Potential biological control of the pupal stage of the European grapevine moth Lobesia botrana by the entomopathogenic fungus Beauveria pseudobassiana in the winter season in Chile

BMC Research Notes ◽

10.1186/s13104-019-4584-6 ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 1

Author(s):

Fabiola Altimira ◽

Nathalia De La Barra ◽

Patricia Rebufel ◽

Sylvana Soto ◽

Rodrigo Soto ◽

...

Keyword(s):

Biological Control ◽

Entomopathogenic Fungus ◽

Winter Season ◽

Pupal Stage ◽

Lobesia Botrana ◽

European Grapevine Moth ◽

Grapevine Moth

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The entomopathogenic fungus, Metarhizium anisopliae for the European grapevine moth, Lobesia botrana Den. & Schiff. (Lepidoptera: Tortricidae) and its effect to the phytopathogenic fungus, Botrytis cinerea

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-018-0086-4 ◽

2018 ◽

Vol 28 (1) ◽

Author(s):

Juan Aguilera Sammaritano ◽

María Deymié ◽

María Herrera ◽

Fabio Vazquez ◽

Andrew G.S. Cuthbertson ◽

...

Keyword(s):

Botrytis Cinerea ◽

Metarhizium Anisopliae ◽

Entomopathogenic Fungus ◽

Lobesia Botrana ◽

Phytopathogenic Fungus ◽

European Grapevine Moth ◽

Grapevine Moth

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European Grapevine Moth and Vitis vinifera L. Phenology in the Douro Region: (A)synchrony and Climate Scenarios

Agronomy ◽

10.3390/agronomy12010098 ◽

2021 ◽

Vol 12 (1) ◽

pp. 98

Author(s):

Samuel Reis ◽

Joana Martins ◽

Fátima Gonçalves ◽

Cristina Carlos ◽

João A. Santos

Keyword(s):

Climate Change ◽

Vitis Vinifera ◽

Climate Change Scenario ◽

Lobesia Botrana ◽

Future Climate ◽

Vitis Vinifera L ◽

Change Scenario ◽

European Grapevine Moth ◽

The Third ◽

Grapevine Moth

The European grapevine moth (Lobesia botrana; Denis and Schiffermüller, 1775) is considered a key pest for grapevine (Vitis vinifera L.) in the Douro Region, Portugal. The phenology of both the grapevine and the pest has changed in the last decades due to the increase in temperature. Here, we assess the potential impact of climate change on the (a)synchrony of both species. The results show that the phenological stages (budburst, flowering and veraison) undergo an advancement throughout the region (at an ~1 km resolution) under a climate change scenario (Representative Concentration Pathways, RCP8.5) for the period 2051–2080, with respect to the historic period (1989–2015). For cv. Touriga Nacional and Touriga Franca, the budburst advances up to 14 days, whereas for flowering and veraison the advancements are up to 10 days (mainly at low elevations along the Douro River). For the phenology of Lobesia botrana, earliness was also verified in the three flights (consequently there may be more generations per year), covering the entire region. Furthermore, the third flight advances further compared to the others. For both varieties, the interaction between the third flight (beginning and peak) and the veraison date is the most relevant modification under the future climate change scenario (RCP8.5, 2051–2080). The aforementioned outcomes from the phenology models help to better understand the possible shifts of both trophic levels in the region under future climate, giving insights into their future interactions.

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Development of Sterile Insect Technique for Control of the European Grapevine Moth, Lobesia botrana, in Urban Areas of Chile

Insects ◽

10.3390/insects12050378 ◽

2021 ◽

Vol 12 (5) ◽

pp. 378

Author(s):

Gregory. S. Simmons ◽

Melissa Cristal Salazar Sepulveda ◽

Edith Alejandra Fuentes Barrios ◽

Marcela Idalsoaga Villegas ◽

Raul Enrique Medina Jimenez ◽

...

Keyword(s):

Urban Areas ◽

Field Studies ◽

Mass Rearing ◽

Fruit Production ◽

Lobesia Botrana ◽

Effective Control ◽

Moth Population ◽

European Grapevine Moth ◽

Grapevine Moth ◽

Production Areas

The European grapevine moth, a Palearctic pest, was first detected in the Americas in 2008. Its establishment in Chile presented production and export issues for grapes and other fruits, and a national control campaign was launched. Urban areas next to agricultural production areas were recognized as a challenge for effective control. In 2015, a SIT laboratory was established in Arica, Chile to evaluate its potential for urban control. Progress included the development and evaluation of artificial diets, a mass-rearing of 75,000 moths/week, confirmation of 150 Gy as an operational dose for inherited sterility, and releases of sterile moths in a 25 ha urban area next to fruit production areas. Season-long releases demonstrated that high overflooding ratios were achieved early in the season but decreased with a large increase in the wild moth population. Sterile moth quality was consistently high, and moths were observed living in the field up to 10 days and dispersing up to 800 m. Recommendations for further development of the SIT include conducting cage and field studies to evaluate overflooding ratios and mating competitiveness, measuring of infestation densities in release and no-release areas, and conducting trials to evaluate combining SIT with compatible integrated pest management (IPM) tactics such as fruit stripping and use of mating disruption.

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Grape variety affects larval performance and also female reproductive performance of the European grapevine moth Lobesia botrana (Lepidoptera: Tortricidae)

Bulletin of Entomological Research ◽

10.1079/ber2005417 ◽

2006 ◽

Vol 96 (2) ◽

pp. 205-212 ◽

Cited By ~ 54

Author(s):

J. Moreau ◽

B. Benrey ◽

D. Thiéry

Keyword(s):

Host Plant ◽

Adult Emergence ◽

Lobesia Botrana ◽

Larval Performance ◽

European Grapevine Moth ◽

Wild Grape ◽

Larval Host ◽

Fitness Index ◽

Grapevine Moth

AbstractFor insect herbivores, the quality of the larval host plant is a key determinant of fitness. Therefore, insect populations are supposed to be positively correlated with the nutritional quality of their host plant. This study aimed to determine if and how different varieties of grapes (including the wild grape Lambrusque) affect both larval and adult performance of the polyphagous European grapevine moth Lobesia botrana (Denis & Schiffermüller). Significant differences were found in larval development time, but not in pupal mass, adult emergence rate, or sex ratio. Although the fecundity of females is not different among varieties, females fed on some varieties produced eggs of different sizes which are correlated to their fertility. Thus, females adapt resource allocation to eggs depending on their diet as larvae. Using a fitness index, the average reproductive output was found to be highest for females reared on cv. Chardonnay. Females reared on wild grape produced a fitness index identical to the cultivated grapes. However, Lambrusque and Gewurztraminer separate themselves from the cultivated varieties according to our discriminant analyses. It is emphasized, through this study, that cultivars fed on by larvae should be considered in the population dynamics of L. botrana and that egg number is insufficient to determine host plant quality.

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