scholarly journals Enhancement of Aeribacillus pallidus lipase production through optimization of medium composition using Box behnken design and its application in detergents formulations

2019 ◽  
Author(s):  
Ameni KTATA ◽  
karray aida ◽  
Ines Mnif ◽  
Sofiane BEZZINE
Keyword(s):  
Waste Water ◽  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Lipase Production ◽  
Maximum Activity ◽  
Medium Composition ◽  
P Value ◽  
Potential Candidate ◽  
Box Behnken Design ◽  
Optimized Conditions

Abstract Background Alkaline, thermostable bacterial lipases are very interested at detergent applications, seen that they replace the use of synthetic detergents which cause substantial environmental problems. These enzymes based detergent are eco friendly and produce a waste water with low level of COD. The present study, investigates a newly isolated Aeribacillus pallidus strain produces, without induction, a novel halophilous, thermo-alkaline and detergent- tolerant lipase. Results Considerable interest has been given to this lipase by the improvement of its production by the optimization of the pH, the (C/N) ratio and the inoculums size, using the response surface methodology based on the Box-Behnken Design of experiments. A total of 16 experiments were conducted, and the optimized pH, (C/N) ratio and inoculums size were 10, 1 and 0,3 respectively. The results of the analysis of variance (ANOVA) test indicated that the established model was significant (p value < 0.05). Conclusions A 6,68-fold of increase in enzyme activity was revealed under the optimized conditions with the maximum activity of 68 U/ml. Additionally, lipase of Aeribacillus pallidus is considered as a potential candidate for applications in detergent formulations since it displayed a good stability towards detergents and wash performance.

scholarly journals Enhancement of Aeribacillus pallidus lipase production through optimization of medium composition using Box behnken design and its application in detergents formulations

2019 ◽  
Author(s):  
Ameni KTATA ◽  
karray aida ◽  
Ines Mnif ◽  
Sofiane BEZZINE
Keyword(s):  
Waste Water ◽  
Response Surface Methodology ◽  
Oxygen Demand ◽  
Lipase Production ◽  
Maximum Activity ◽  
Medium Composition ◽  
P Value ◽  
Potential Candidate ◽  
Box Behnken Design ◽  
Optimized Conditions

Abstract Background : Alkaline, thermostable bacterial lipases are very interested at detergent applications, seen that they replace the use of synthetic detergents which cause substantial environmental problems. These enzymes based detergent are eco friendly and produce a waste water with low level of COD (Chemical Oxygen Demand). The present study, investigates a newly isolated Aeribacillus pallidus strain produces, without induction, a novel halophilous, thermo-alkaline and detergent- tolerant lipase. Results: Considerable interest has been given to this lipase by the improvement of its production by the optimization of the pH, the (C/N) ratio and the inoculums size, using the response surface methodology based on the Box-Behnken Design of experiments. A total of 16 experiments were conducted, and the optimized pH, (C/N) ratio and inoculums size were 10, 1 and 0.3 respectively. The results of the analysis of variance (ANOVA) test indicated that the established model was significant (p value < 0.05). Conclusions: A 6.68-fold of increase in enzyme activity was revealed under the optimized conditions with the maximum activity of 68 U/mL. Additionally, lipase of Aeribacillus pallidus is considered as a potential candidate for applications in detergent formulations since it displayed a good stability towards detergents and wash performance.

scholarly journals Enhancement of Aeribacillus pallidus lipase production through optimization of medium composition using Box behnken design and its application in detergents formulations

2019 ◽  
Author(s):  
Ameni KTATA ◽  
karray aida ◽  
Ines Mnif ◽  
adel Sayari ◽  
Sofiane BEZZINE
Keyword(s):  
Waste Water ◽  
Response Surface Methodology ◽  
Catalytic Activity ◽  
Oxygen Demand ◽  
Lipase Production ◽  
Medium Composition ◽  
P Value ◽  
Potential Candidate ◽  
Box Behnken Design ◽  
Production Conditions

Abstract Background : Alkaline, thermostable bacterial lipases are largely used in detergent applications, since they substitute the use of synthetic detergents which are known to cause substantial environmental problems. These enzymes based detergent are eco friendly and produce a waste water with low level of COD (Chemical Oxygen Demand). In the present study, we investigated a newly isolated Aeribacillus pallidus strain produces, without induction, a novel halophilous, thermo-alkaline and detergent- tolerant lipase. Results: Considerable interest has been given to this lipase by the improvement of its catalytic activity through the optimization of the pH, the (C/N) ratio and the inoculums size, using the response surface methodology based on the Box-Behnken Design of experiments. A total of 16 experiments were conducted, and the optimized pH, (C/N) ratio and inoculums size were 10, 1 and 0.3 respectively. The results of the analysis of variance (ANOVA) test indicated that the established model was significant (p value < 0.05). Conclusions: The optimization of the production conditions leads to 6.68-fold of increase in the catalytic activity with a maximum of 68 U/mL. All in all, the lipase of Aeribacillus pallidus could be considered as a potential candidate to be incorporated in detergent formulations since it shows a good stability towards detergents and wash performance.

Process Improvisation Strategies for the Enhancement of Lipase Activity

2021 ◽  
Vol 16 (7) ◽  
pp. 114-121
Author(s):  
Prama Das ◽  
Soham Chattopadhyay
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Olive Oil ◽  
Specific Activity ◽  
Lipase Production ◽  
Maximum Activity ◽  
Statistical Optimization ◽  
Production Medium ◽  
High Enzyme ◽  
High Enzyme Activity

In this present study, lipase-producing bacteria were isolated and screened from an indigenous soil sample and were used for lipase production with high enzyme activity. In the production medium, different production media were screened and lipase production was induced by olive oil, 14 mL/L. It was observed from Luedeking and Piret model that the lipase production was mixed growth associated with maximum activity at 37°C and at pH 7. Statistical optimization using Response Surface Methodology was performed to understand the interaction of different parameters and the standardized conditions obtained were as follows: Peptone 10 g/L, yeast extract 7.5 g/L and olive oil 14 mL/L. The predicted data were validated and the model predicted was significant with a maximum specific activity of 1.1 µmole/min/mg proteins. The lipasespecific activity was enhanced by 10% and 23% after a single parameter and statistical optimization.

scholarly journals Statistical Optimization of Parameters for the Enhancement of Lipase Activity

2020 ◽  
Author(s):  
Prama Das ◽  
Soham Chattopadhyay
Keyword(s):  
Response Surface Methodology ◽  
Olive Oil ◽  
Specific Activity ◽  
Lipase Production ◽  
Maximum Activity ◽  
Statistical Optimization ◽  
Production Medium ◽  
Optimization Of Parameters ◽  
High Enzyme ◽  
High Enzyme Activity

Abstract In this present study, lipase producing bacteria were isolated and screened from an indigenous soil sample and was used for lipase production with high enzyme activity. Different production media were screened and lipase production was found to be induced by olive oil, 14 mL/L, in the production medium. It was observed from Luedeking and Piret model that the lipase production was mixed growth associated with its maximum activity at 37 °C and pH 7. To understand the interaction of different parameters, statistical optimization using Response Surface Methodology was performed and the standardized conditions obtained were as follows: Peptone 10 g/L, yeast extract 7.5 g/L and olive oil 14 mL/L. The predicted data were validated and the model predicted was significant with maximum specific activity of 1.1 µmole/min/mg proteins. The lipase specific activity was found to be enhanced by 10% and 23% after a single parameter and statistical optimization respectively.

scholarly journals Statistical Optimization of Copper Oxide Nanoparticles Using Response Surface Methodology and Box–Behnken Design Towards In Vitro and In Vivo Toxicity Assessment

2020 ◽  
Vol 11 (3) ◽  
pp. 10027-10039
Keyword(s):  
Response Surface Methodology ◽  
Copper Oxide ◽  
Response Surface ◽  
Copper Oxide Nanoparticles ◽  
Toxicity Assessment ◽  
P Value ◽  
Oxide Nanoparticles ◽  
Box Behnken Design

The current study focusses on the optimization of Copper oxide nanoparticles (CuO NPs) biosynthesis with Alternanthera sessilis (L.) extract using response surface methodology (RSM). The effect of time, pH, and extract to metal concentration ratio on the yield of synthesized nanoparticles (NPs) were estimated using Box–Behnken design. The influence of each of the parameters, as mentioned earlier, was determined by synthesizing nanoparticles under different conditions. A total of 29 experimental runs were carried out to estimate the crucial parameters. Extract to the metal ratio was found to be the vital parameter for yield optimization based on the p-values (p-value < 0.05). The physicochemical property of NPs, like size, was estimated to be in the range of 10-20 nm. In zebrafish, 48 hpf and 72 hpf were measured at 90 µM to reduce dysfunction and mortality during organ development. These results can have a valuable impact on eco-toxicological effects.

Optimization of Liquid Culture Conditions of Methamidophos Degrading Enzyme from Staphylococcus rosehbach

2013 ◽  
Vol 303-306 ◽  
pp. 2683-2686 ◽  
Author(s):  
Na Zhang ◽  
Qing Qi Guo ◽  
Xiaofei Liu ◽  
Kun Song ◽  
Shuang Wang ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Response Surface ◽  
Liquid Culture ◽  
Soybean Protein ◽  
Culture Conditions ◽  
Box Behnken Design ◽  
Degrading Enzyme ◽  
Culture Time ◽  
Degradation Ratio

The nutritional parameters of methamidophos degrading enzyme, which is isolated from soil from Daqing and identified as Staphylococcus Rosehbach, were studied by response surface methodology (RSM). Glucose, peptone and culture time were analyzed by Box-Behnken design for optimizing their concentrations in the medium and the effect of their mutual interaction on methamidophos degrading enzyme activity. Glucose and soybean protein were found in influencing the methamidophos degrading enzyme activity highly significant. Glucose and soybean protein also showed an effect on the methamidophos degrading enzyme activity in interaction with other variables. The optima values of the parameters obtained through RSM, 0.31% glucose, 0.09% peptone and culture time 72h were applied. Actual value of verifiable experimentation was that the degradation ratio of methamidophos degrading enzyme to methamidophos was 39.8% at the optima conditions.

Statistical Optimization for Dye Removal from Aqueous Solution by Cross-linked Chitosan Composite

2020 ◽  
Vol 14 (2) ◽  
pp. 1 ◽  
Author(s):  
Ali Jawad ◽  
Mohd shafiq Bin Aazmi ◽  
Ali Jawad
Keyword(s):  
Aqueous Solution ◽  
Response Surface Methodology ◽  
Methyl Orange ◽  
Response Surface ◽  
Removal Efficiency ◽  
Dye Removal ◽  
Statistical Optimization ◽  
P Value ◽  
Box Behnken Design ◽  
Chitosan Composite

Response surface methodology-Box–Behnken design (RSM-BBD) was employed to optimize the methyl orange (MO) dye removal efficiency from aqueous solution by cross-linked chitosan-tripolyphosphate/nano-titania composite (Chi-TPP/NTC). The influence of pertinent parameters, i.e. A: TiO2 loading (0- 50 %), B: dose (0.04-0.14 g), C: pH (4-10), and D: temperature (30-50 oC) on the MO removal efficiency were tested and optimized using RSM-BBD. The F-values of BBD model for MO removal efficiency was 93.4 (corresponding p-value < 0.0001). The results illustrated that the highest MO removal efficiency (87.27 %) was observed at the following conditions: TiO2 loading (50% TiO2), dose (0.09 g), pH 4.0, and temperature of 40 oC.  

scholarly journals Organosolvent Extraction Coupled with Steam Explosion Pretreatment of Wild Sugarcane and Optimization of Cellulase Activity using Response Surface Methodology

2021 ◽  
Vol 15 (1) ◽  
pp. 114-122
Author(s):  
S. Aruna ◽  
Sriramulu Gobikrishnan
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Response Surface ◽  
Steam Explosion ◽  
Cellulase Activity ◽  
Enzyme Loading ◽  
Box Behnken Design ◽  
Cellulase Enzyme ◽  
Steam Explosion Pretreatment ◽  
Optimal Ph

The present study focuses on the effect of the organosolvent extraction with steam explosion pretreatment and optimization of cellulase activity using the response surface methodology for the production of bioethanol. The commercial cellulase enzyme was characterized for its optimal pH, temperature, and also studied for the enzyme activity. The effect of organosolvent pretreatment was identified in both native and pretreated wild sugarcane by SEM, XRD, and FTIR. The cellulase enzyme was found to be tolerant with an optimal pH of 5.0 with cellulase activity of pretreated wild sugarcane is found to be 25.36 IU/ml. Organosolvent extraction of wild sugarcane was carried out using water and ethanol as solvent. The composition of lignin, hemicelluloses, and cellulose was also determined in both pretreated and native biomass. The activity of the enzyme was observed over the effect of enzyme loading, substrate loading, temperature, and pH using a Box-Behnken design.

scholarly journals Comparison of Lipase Production by Enterococcus faecium MTCC 5695 and Pediococcus acidilactici MTCC 11361 Using Fish Waste as Substrate: Optimization of Culture Conditions by Response Surface Methodology

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Vrinda Ramakrishnan ◽  
Louella Concepta Goveas ◽  
Bhaskar Narayan ◽  
Prakash M. Halami
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enterococcus Faecium ◽  
Protein Hydrolysate ◽  
Lipase Production ◽  
Culture Conditions ◽  
Pediococcus Acidilactici ◽  
Fish Waste ◽  
Fermentation Time ◽  
Optimized Conditions

A medium using fish waste as substrate was designed for production of lipase by Enterococcus faecium MTCC 5695 and Pediococcus acidilactici MTCC 11361. Medium components and culture conditions (fish waste protein hydrolysate (FWPH) concentration, fish waste oil (FWO) concentration, pH, temperature, and fermentation time) which affect lipase production were screened using factorial (5 factors ∗ 2 levels) design of which FWPH concentration, FWO concentration, and fermentation time showed significance (). The levels of these factors were optimized further by Box-Behnken design using response surface methodology (RSM). Optimized conditions were found to be 5% v/v FWO, 0.15 mg/mL FWPH and 24 h of fermentation time for MTCC 5695, and 4% v/v FWO, 0.15 mg/mL FWPH and 24 h of fermentation for MTCC 11361, which were further validated. Under optimized conditions, MTCC 5695 and MTCC 11361 showed 3.15- (543.63 to 1715 U/mL) and 2.3- (214.74 to 493 U/mL) fold increase in lipase production, respectively, as compared to unoptimized conditions.

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