scholarly journals Statistical Optimization of Parameters for the Enhancement of Lipase Activity

2020 ◽  
Author(s):  
Prama Das ◽  
Soham Chattopadhyay
Keyword(s):  
Response Surface Methodology ◽  
Olive Oil ◽  
Specific Activity ◽  
Lipase Production ◽  
Maximum Activity ◽  
Statistical Optimization ◽  
Production Medium ◽  
Optimization Of Parameters ◽  
High Enzyme ◽  
High Enzyme Activity

Abstract In this present study, lipase producing bacteria were isolated and screened from an indigenous soil sample and was used for lipase production with high enzyme activity. Different production media were screened and lipase production was found to be induced by olive oil, 14 mL/L, in the production medium. It was observed from Luedeking and Piret model that the lipase production was mixed growth associated with its maximum activity at 37 °C and pH 7. To understand the interaction of different parameters, statistical optimization using Response Surface Methodology was performed and the standardized conditions obtained were as follows: Peptone 10 g/L, yeast extract 7.5 g/L and olive oil 14 mL/L. The predicted data were validated and the model predicted was significant with maximum specific activity of 1.1 µmole/min/mg proteins. The lipase specific activity was found to be enhanced by 10% and 23% after a single parameter and statistical optimization respectively.

Process Improvisation Strategies for the Enhancement of Lipase Activity

2021 ◽  
Vol 16 (7) ◽  
pp. 114-121
Author(s):  
Prama Das ◽  
Soham Chattopadhyay
Keyword(s):  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Olive Oil ◽  
Specific Activity ◽  
Lipase Production ◽  
Maximum Activity ◽  
Statistical Optimization ◽  
Production Medium ◽  
High Enzyme ◽  
High Enzyme Activity

In this present study, lipase-producing bacteria were isolated and screened from an indigenous soil sample and were used for lipase production with high enzyme activity. In the production medium, different production media were screened and lipase production was induced by olive oil, 14 mL/L. It was observed from Luedeking and Piret model that the lipase production was mixed growth associated with maximum activity at 37°C and at pH 7. Statistical optimization using Response Surface Methodology was performed to understand the interaction of different parameters and the standardized conditions obtained were as follows: Peptone 10 g/L, yeast extract 7.5 g/L and olive oil 14 mL/L. The predicted data were validated and the model predicted was significant with a maximum specific activity of 1.1 µmole/min/mg proteins. The lipasespecific activity was enhanced by 10% and 23% after a single parameter and statistical optimization.

scholarly journals Enhancement of Aeribacillus pallidus lipase production through optimization of medium composition using Box behnken design and its application in detergents formulations

2019 ◽  
Author(s):  
Ameni KTATA ◽  
karray aida ◽  
Ines Mnif ◽  
Sofiane BEZZINE
Keyword(s):  
Waste Water ◽  
Response Surface Methodology ◽  
Enzyme Activity ◽  
Lipase Production ◽  
Maximum Activity ◽  
Medium Composition ◽  
P Value ◽  
Potential Candidate ◽  
Box Behnken Design ◽  
Optimized Conditions

Abstract Background Alkaline, thermostable bacterial lipases are very interested at detergent applications, seen that they replace the use of synthetic detergents which cause substantial environmental problems. These enzymes based detergent are eco friendly and produce a waste water with low level of COD. The present study, investigates a newly isolated Aeribacillus pallidus strain produces, without induction, a novel halophilous, thermo-alkaline and detergent- tolerant lipase. Results Considerable interest has been given to this lipase by the improvement of its production by the optimization of the pH, the (C/N) ratio and the inoculums size, using the response surface methodology based on the Box-Behnken Design of experiments. A total of 16 experiments were conducted, and the optimized pH, (C/N) ratio and inoculums size were 10, 1 and 0,3 respectively. The results of the analysis of variance (ANOVA) test indicated that the established model was significant (p value < 0.05). Conclusions A 6,68-fold of increase in enzyme activity was revealed under the optimized conditions with the maximum activity of 68 U/ml. Additionally, lipase of Aeribacillus pallidus is considered as a potential candidate for applications in detergent formulations since it displayed a good stability towards detergents and wash performance.

scholarly journals Statistical Optimization, Partial Purification, and Characterization of Phytase Produced from Talaromyces purpureogenus NSA20 Using Potato Peel Waste and its Application in Dyes De-colorization

2021 ◽  
Vol 12 (4) ◽  
pp. 4417-4431
Keyword(s):  
Crystal Violet ◽  
Specific Activity ◽  
Marine Fungus ◽  
Maximum Activity ◽  
Statistical Optimization ◽  
Partial Purification ◽  
Methyl Red ◽  
Potato Peel ◽  
Phytase Production

In this study, Talaromyces purpureogenus NSA20 as phytase-producing marine fungus was isolated and identified morphologically and genetically and deposited in Gene Bank with accession number MW031769.1. One factor at a time (OFAT) optimization was performed, where the result revealed that potato peel waste (1.5%) as a substrate was the highest for phytase production at 6 days, where the maximum activity of phytase was 138.4 U/ml. Moreover, Box–Behnken design as response surface methodology was carried out for statistical optimization of phytase production by T. purpureogenus NSA20. Statistical optimization illustrated that the optimized medium for phytase production increased 1.57 fold compared to the OFAT optimized medium. Partial purification of phytase was carried out, where the enzyme after precipitation with ammonium sulfate (80%) was 2.6-fold purified phytase, and the yield was 39.8 %., the specific activity was 31.19 U/mg proteins. Additionally, partially purified phytase was characterized; the maximum activity of phytase at Fe++ 0.1% and pH 5.5 at 37 oC was 350 U/ml. Eventually, phytase was applied for crystal violet and methyl red decolorization, where decolorization percentages of crystal violet and methyl red were 85.5% and 75% at 120 min, respectively.

scholarly journals Enhancement of Aeribacillus pallidus lipase production through optimization of medium composition using Box behnken design and its application in detergents formulations

2019 ◽  
Author(s):  
Ameni KTATA ◽  
karray aida ◽  
Ines Mnif ◽  
Sofiane BEZZINE
Keyword(s):  
Waste Water ◽  
Response Surface Methodology ◽  
Oxygen Demand ◽  
Lipase Production ◽  
Maximum Activity ◽  
Medium Composition ◽  
P Value ◽  
Potential Candidate ◽  
Box Behnken Design ◽  
Optimized Conditions

Abstract Background : Alkaline, thermostable bacterial lipases are very interested at detergent applications, seen that they replace the use of synthetic detergents which cause substantial environmental problems. These enzymes based detergent are eco friendly and produce a waste water with low level of COD (Chemical Oxygen Demand). The present study, investigates a newly isolated Aeribacillus pallidus strain produces, without induction, a novel halophilous, thermo-alkaline and detergent- tolerant lipase. Results: Considerable interest has been given to this lipase by the improvement of its production by the optimization of the pH, the (C/N) ratio and the inoculums size, using the response surface methodology based on the Box-Behnken Design of experiments. A total of 16 experiments were conducted, and the optimized pH, (C/N) ratio and inoculums size were 10, 1 and 0.3 respectively. The results of the analysis of variance (ANOVA) test indicated that the established model was significant (p value < 0.05). Conclusions: A 6.68-fold of increase in enzyme activity was revealed under the optimized conditions with the maximum activity of 68 U/mL. Additionally, lipase of Aeribacillus pallidus is considered as a potential candidate for applications in detergent formulations since it displayed a good stability towards detergents and wash performance.

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