Group B Streptococcus detection in pregnant women: comparison of real-time PCR assay, culture, and the Xpert GBS rapid test

Abstract Background: Group B Streptococcus (GBS) is one of the most important causative agents of neonatal sepsis. As administration of prophylactic antibiotics during labor can prevent GBS infection, routine screening for this bacterium in prenatal care before the onset of labor is recommended. However, many women present in labor without having undergone such testing during antenatal care, and the turnaround time of detection methods is insufficient for results to be obtained before delivery. Methods: Vaginal and anorectal specimens were collected from 270 pregnant women. Each sample was tested by Xpert GBS, qPCR, and culture for GBS detection. Results: The overall prevalence of maternal GBS colonization was 30.7% according to Xpert GBS, 51.1% according to qPCR, and 14.3% according to cultures. Considering the qPCR method as the reference, the Xpert GBS had a sensitivity of 53% and specificity of 93%. Positive Xpert GBS results were correlated to marital status (married or cohabitating) and with prematurity as a cause of neonatal hospitalization. Positive cultures were related with ischemic–hypoxic encephalopathy requiring therapeutic hypothermia. Conclusions: Combined enrichment/qPCR and the Xpert GBS rapid test found a high prevalence of GBS colonization. The Xpert GBS technique gives faster results and could be useful for evaluating mothers who present without antenatal GBS screening results and are at risk of preterm labor, thus allowing institution of prophylactic antibiotic therapy. Keywords: Group B Streptococcus ; Streptococcus agalactiae ; Xpert GBS; real-time polymerase chain reaction; antenatal care

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Group B Streptococcus detection in pregnant women: comparison of real-time PCR assay, culture, and the Xpert GBS rapid test

10.21203/rs.2.11969/v5 ◽

2019 ◽

Author(s):

Laura Vieira ◽

Amanda Vilaverde Perez ◽

Monique M Machado ◽

Michele L Kayser ◽

Daniela V Vettori ◽

...

Keyword(s):

Antenatal Care ◽

Pregnant Women ◽

Real Time ◽

Group B Streptococcus ◽

Prophylactic Antibiotic ◽

Rapid Test ◽

Turnaround Time ◽

Detection Methods ◽

Causative Agents ◽

Group B

Abstract Background: Group B Streptococcus (GBS) is one of the most important causative agents of neonatal sepsis. As administration of prophylactic antibiotics during labor can prevent GBS infection, routine screening for this bacterium in prenatal care before the onset of labor is recommended. However, many women present in labor without having undergone such testing during antenatal care, and the turnaround time of detection methods is insufficient for results to be obtained before delivery. Methods: Vaginal and anorectal specimens were collected from 270 pregnant women. Each sample was tested by Xpert GBS, qPCR, and culture for GBS detection. Results: The overall prevalence of maternal GBS colonization was 30.7% according to Xpert GBS, 51.1% according to qPCR, and 14.3% according to cultures. Considering the qPCR method as the reference, the Xpert GBS had a sensitivity of 53% and specificity of 93%. Positive Xpert GBS results were correlated to marital status (married or cohabitating) and with prematurity as a cause of neonatal hospitalization. Positive cultures were related with ischemic–hypoxic encephalopathy requiring therapeutic hypothermia. Conclusions: Combined enrichment/qPCR and the Xpert GBS rapid test found a high prevalence of GBS colonization. The Xpert GBS technique gives faster results and could be useful for evaluating mothers who present without antenatal GBS screening results and are at risk of preterm labor, thus allowing institution of prophylactic antibiotic therapy. Keywords: Group B Streptococcus; Streptococcus agalactiae; Xpert GBS; real-time polymerase chain reaction; antenatal care

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Group B Streptococcus detection in pregnant women: comparison of real-time PCR assay, culture, and the Xpert GBS rapid test

10.21203/rs.2.11969/v1 ◽

2019 ◽

Author(s):

Laura Vieira ◽

Amanda Vilaverde Perez ◽

Monique M Machado ◽

Michele L Kayser ◽

Daniela V Vettori ◽

...

Keyword(s):

Pregnant Women ◽

Group B Streptococcus ◽

Prophylactic Antibiotic ◽

Rapid Test ◽

Turnaround Time ◽

Detection Methods ◽

Institutional Research ◽

Delivery Methods ◽

Causative Agents ◽

Group B

Abstract Background Group B Streptococcus (GBS) is one of the most important causative agents of neonatal sepsis. As administration of prophylactic antibiotics during labor can prevent GBS infection, routine screening for this bacterium in prenatal care before the onset of labor is recommended. However, many women present in labor without having undergone such testing during antenatal care, and the turnaround time of detection methods is insufficient for results to be obtained before delivery. Methods Vaginal and anorectal specimens were collected from 270 pregnant women. Each sample was tested by Xpert GBS, PCR, and culture for GBS detection. Results The overall prevalence of maternal GBS colonization was 30.7% according to Xpert GBS, 51.1% according to PCR, and 14.3% according to cultures. Considering the PCR method as the reference, the Xpert GBS had a sensitivity of 53% and specificity of 93%. Positive Xpert GBS results were associated with marital status (married or cohabitating) and with prematurity as a cause of neonatal hospitalization. Positive cultures were associated with ischemic–hypoxic encephalopathy requiring therapeutic hypothermia. Conclusions Combined enrichment/PCR and the Xpert GBS rapid test found a high prevalence of GBS colonization. The Xpert GBS technique gives faster results and could be useful for evaluating mothers who present without antenatal GBS screening results and are at risk of preterm labor, thus allowing institution of prophylactic antibiotic therapy. Trial Registration: The study was approved by the institutional Research Ethics Committee (CAAE: 59688316.0.0000.5327)

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Group B Streptococcus detection in pregnant women: comparison of real-time PCR assay, culture, and the Xpert GBS rapid test

10.21203/rs.2.11969/v3 ◽

2019 ◽

Author(s):

Laura Vieira ◽

Amanda Vilaverde Perez ◽

Monique M Machado ◽

Michele L Kayser ◽

Daniela V Vettori ◽

...

Keyword(s):

Pregnant Women ◽

Group B Streptococcus ◽

Prophylactic Antibiotic ◽

Rapid Test ◽

Turnaround Time ◽

Detection Methods ◽

Institutional Research ◽

Delivery Methods ◽

Causative Agents ◽

Group B

Abstract Background Group B Streptococcus (GBS) is one of the most important causative agents of neonatal sepsis. As administration of prophylactic antibiotics during labor can prevent GBS infection, routine screening for this bacterium in prenatal care before the onset of labor is recommended. However, many women present in labor without having undergone such testing during antenatal care, and the turnaround time of detection methods is insufficient for results to be obtained before delivery. Methods Vaginal and anorectal specimens were collected from 270 pregnant women. Each sample was tested by Xpert GBS, PCR, and culture for GBS detection. Results The overall prevalence of maternal GBS colonization was 30.7% according to Xpert GBS, 51.1% according to PCR, and 14.3% according to cultures. Considering the PCR method as the reference, the Xpert GBS had a sensitivity of 53% and specificity of 93%. Positive Xpert GBS results were associated with marital status (married or cohabitating) and with prematurity as a cause of neonatal hospitalization. Positive cultures were associated with ischemic–hypoxic encephalopathy requiring therapeutic hypothermia. Conclusions Combined enrichment/PCR and the Xpert GBS rapid test found a high prevalence of GBS colonization. The Xpert GBS technique gives faster results and could be useful for evaluating mothers who present without antenatal GBS screening results and are at risk of preterm labor, thus allowing institution of prophylactic antibiotic therapy. Trial Registration: The study was approved by the institutional Research Ethics Committee (CAAE: 59688316.0.0000.5327)

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Group B Streptococcus detection in pregnant women: comparison of qPCR assay, culture, and the Xpert GBS rapid test

BMC Pregnancy and Childbirth ◽

10.1186/s12884-019-2681-0 ◽

2019 ◽

Vol 19 (1) ◽

Cited By ~ 2

Author(s):

Laura L. Vieira ◽

Amanda V. Perez ◽

Monique M. Machado ◽

Michele L. Kayser ◽

Daniela V. Vettori ◽

...

Keyword(s):

Pregnant Women ◽

Group B Streptococcus ◽

Prophylactic Antibiotic ◽

Rapid Test ◽

Turnaround Time ◽

Detection Methods ◽

High Prevalence ◽

Causative Agents ◽

Prophylactic Antibiotic Therapy ◽

Group B

Abstract Background Group B Streptococcus (GBS) is one of the most important causative agents of neonatal sepsis. As administration of prophylactic antibiotics during labor can prevent GBS infection, routine screening for this bacterium in prenatal care before the onset of labor is recommended. However, many women present in labor without having undergone such testing during antenatal care, and the turnaround time of detection methods is insufficient for results to be obtained before delivery. Methods Vaginal and anorectal specimens were collected from 270 pregnant women. Each sample was tested by Xpert GBS, qPCR, and culture for GBS detection. Results The overall prevalence of maternal GBS colonization was 30.7% according to Xpert GBS, 51.1% according to qPCR, and 14.3% according to cultures. Considering the qPCR method as the reference, the Xpert GBS had a sensitivity of 53% and specificity of 93%. Positive Xpert GBS results were correlated to marital status (married or cohabitating) and with prematurity as a cause of neonatal hospitalization. Positive cultures were related with ischemic–hypoxic encephalopathy requiring therapeutic hypothermia. Conclusions Combined enrichment/qPCR and the Xpert GBS rapid test found a high prevalence of GBS colonization. The Xpert GBS technique gives faster results and could be useful for evaluating mothers who present without antenatal GBS screening results and are at risk of preterm labor, thus allowing institution of prophylactic antibiotic therapy.

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Group B Streptococcus detection in pregnant women: comparison of real-time PCR assay, culture, and the Xpert GBS rapid test

10.21203/rs.2.11969/v2 ◽

2019 ◽

Author(s):

Laura Vieira ◽

Amanda Vilaverde Perez ◽

Monique M Machado ◽

Michele L Kayser ◽

Daniela V Vettori ◽

...

Keyword(s):

Pregnant Women ◽

Group B Streptococcus ◽

Prophylactic Antibiotic ◽

Rapid Test ◽

Turnaround Time ◽

Detection Methods ◽

Institutional Research ◽

Delivery Methods ◽

Causative Agents ◽

Group B

Abstract Background Group B Streptococcus (GBS) is one of the most important causative agents of neonatal sepsis. As administration of prophylactic antibiotics during labor can prevent GBS infection, routine screening for this bacterium in prenatal care before the onset of labor is recommended. However, many women present in labor without having undergone such testing during antenatal care, and the turnaround time of detection methods is insufficient for results to be obtained before delivery. Methods Vaginal and anorectal specimens were collected from 270 pregnant women. Each sample was tested by Xpert GBS, PCR, and culture for GBS detection. Results The overall prevalence of maternal GBS colonization was 30.7% according to Xpert GBS, 51.1% according to PCR, and 14.3% according to cultures. Considering the PCR method as the reference, the Xpert GBS had a sensitivity of 53% and specificity of 93%. Positive Xpert GBS results were associated with marital status (married or cohabitating) and with prematurity as a cause of neonatal hospitalization. Positive cultures were associated with ischemic–hypoxic encephalopathy requiring therapeutic hypothermia. Conclusions Combined enrichment/PCR and the Xpert GBS rapid test found a high prevalence of GBS colonization. The Xpert GBS technique gives faster results and could be useful for evaluating mothers who present without antenatal GBS screening results and are at risk of preterm labor, thus allowing institution of prophylactic antibiotic therapy. Trial Registration: The study was approved by the institutional Research Ethics Committee (CAAE: 59688316.0.0000.5327)

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Group B Streptococcus Colonization among Pregnant Women Attending Antenatal Care at Tertiary Hospital in Rural Southwestern Uganda

International Journal of Microbiology ◽

10.1155/2016/3816184 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 12

Author(s):

Abdul Namugongo ◽

Joel Bazira ◽

Yarine Fajardot ◽

Ngonzi Joseph

Keyword(s):

Antenatal Care ◽

Pregnant Women ◽

Univariate Analysis ◽

Group B Streptococcus ◽

Antenatal Clinic ◽

Tertiary Hospital ◽

Cross Sectional Study ◽

Cross Sectional ◽

Group B ◽

Southwestern Uganda

Objectives.This study sought to determine the prevalence and factors associated with group B streptococcal anogenital colonization among pregnant women attending antenatal care at Mbarara Regional Referral Hospital, a tertiary hospital.Methods.Cross-sectional study where 309 pregnant women ≥ thirty-five weeks of gestation attending antenatal clinic were consecutively recruited between January and March 2015. Anovaginal swabs were collected and tested qualitatively using rapid visual immunoassay GBS test kits for presence of GBS antigens. Data was analyzed using STATA version 12. In univariate analysis, GBS colonized mothers were presented as percentages and numbers, and in multivariate analysis logistic regression analysis was applied to determine the associations of exposure variable and GBS colonization; a value of less than 0.05 was considered significant.Results.Mothers’ median age was 25 years, 14.6% mothers being obese. GBS prevalence was 28.8%, 95% CI: 23.7–33.9. Obesity was the only significant factor associated with anogenital GBS colonization with odds ratio of 3.78, 95% CI: 1.78–8.35, apvalue of 0.001. Maternal ages, educational level, residence, and gravidity were not associated with GBS anogenital colonization.Conclusion.Group B streptococcal anogenital colonization among pregnant women attending antenatal care at tertiary hospital, in Southwestern Uganda, is high.

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