IgE reactivity to fish allergens from Pacific cod (Gadus macrocephalus) in atopic dogs

Abstract Background: IgE reactivity to fish allergens in atopic dogs, which are used as models for food allergy, has not been elucidated to date. We investigated IgE reactivity to crude extracts and purified allergens derived from the Pacific cod (Gadus macrocephalus) in atopic dogs to identify the allergenic proteins of cod.Results: The levels of specific IgE to crude cod extracts were measured in the sera of 179 atopic dogs, including 27 dogs with cod allergy, using enzyme-linked immunosorbent assay (ELISA). Specific IgE to crude cod extracts were present in 36 (20%) of the 179 atopic dogs and in 12 (44%) of the 27 dogs with cod allergy. The allergens in crude cod extracts were analyzed by ELISA, immunoblotting, and liquid chromatography-tandem mass spectrometry. In allergen component analysis, IgE reactivity to tropomyosin and enolase was observed in the sera of dogs with cod allergy. IgE reactivity to parvalbumin, collagen, and tropomyosin was evaluated using the sera of atopic dogs that tested positive for specific IgE to crude cod extracts. Among the 36 dogs with IgE reactivity to crude cod extracts, 9 (25%), 14 (39%), and 18 (50%) dogs tested positive for specific IgE to parvalbumin, collagen, and tropomyosin, respectively.Conclusions: The IgE reactivity to cod allergens observed in dogs was similar to that in humans, and this finding further supports the use of atopic dogs with fish allergy as a model for fish allergy in humans.

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IgE reactivity to fish allergens from Pacific cod (Gadus macrocephalus) in atopic dogs

BMC Veterinary Research ◽

10.1186/s12917-020-02559-1 ◽

2020 ◽

Vol 16 (1) ◽

Author(s):

Ichiro Imanishi ◽

Jumpei Uchiyama ◽

Keijiro Mizukami ◽

Junichi Kamiie ◽

Keigo Kurata ◽

...

Keyword(s):

Specific Ige ◽

Enzyme Linked Immunosorbent Assay ◽

Pacific Cod ◽

Fish Allergy ◽

Ige Reactivity ◽

Pacific Cod Gadus Macrocephalus ◽

Gadus Macrocephalus ◽

The Pacific ◽

Allergenic Proteins ◽

Liquid Chromatography Tandem Mass

Abstract Background IgE reactivity to fish allergens in atopic dogs, which are used as models for food allergy, has not been elucidated to date. We investigated IgE reactivity to crude extracts and purified allergens derived from the Pacific cod (Gadus macrocephalus) in atopic dogs to identify the allergenic proteins of cod. Results The levels of specific IgE to crude cod extracts were measured in the sera of 179 atopic dogs, including 27 dogs with cod allergy, using enzyme-linked immunosorbent assay (ELISA). Specific IgE to crude cod extracts were present in 36 (20%) of the 179 atopic dogs and in 12 (44%) of the 27 dogs with cod allergy. The allergens in crude cod extracts were analyzed by ELISA, immunoblotting, and liquid chromatography-tandem mass spectrometry. In allergen component analysis, IgE reactivity to tropomyosin and enolase was observed in the sera of dogs with cod allergy. IgE reactivity to parvalbumin, collagen, and tropomyosin was evaluated using the sera of atopic dogs that tested positive for specific IgE to crude cod extracts. Among the 36 dogs with IgE reactivity to crude cod extracts, 9 (25%), 14 (39%), and 18 (50%) dogs tested positive for specific IgE to parvalbumin, collagen, and tropomyosin, respectively. Conclusions The IgE reactivity to cod allergens observed in dogs was similar to that in humans, and this finding further supports the use of atopic dogs with fish allergy as a model for fish allergy in humans.

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IgE reactivity to fish allergens in Pacific cod (Gadus macrocephalus) in atopic dogs

10.21203/rs.2.19058/v2 ◽

2020 ◽

Author(s):

Ichiro Imanishi ◽

Jumpei Uchiyama ◽

Keijiro Mizukami ◽

Junichi Kamiie ◽

Keigo Kurata ◽

...

Keyword(s):

Specific Ige ◽

Enzyme Linked Immunosorbent Assay ◽

Pacific Cod ◽

Fish Allergy ◽

Ige Reactivity ◽

Pacific Cod Gadus Macrocephalus ◽

Chromatography Tandem Mass Spectrometry ◽

Gadus Macrocephalus ◽

Allergenic Proteins ◽

Liquid Chromatography Tandem Mass

Abstract Background: IgE reactivity to fish allergens in atopic dogs, which are used as models for food allergy, has not been elucidated to date. We investigated IgE reactivity to crude extracts and purified Pacific cod (Gadus macrocephalus) allergens in atopic dogs to identify the allergenic proteins of cod.Methods: Specific IgE to crude cod extracts in the sera of 179 atopic dogs, including 27 dogs with cod allergy, were measured using enzyme-linked immunosorbent assay (ELISA). The allergens of crude cod extracts were analyzed by ELISA, immunoblotting, and liquid chromatography-tandem mass spectrometry (LC-MS/MS). IgE reactivity to parvalbumin, collagen, and tropomyosin was evaluated using the sera of atopic dogs that were positive for specific IgE to crude cod extracts.Results: Specific IgE to crude cod extracts were present in 36 (20%) of the 179 atopic dogs and 12 (44%) of the 27 dogs with cod allergy. In allergen component analysis, IgE reactivity to tropomyosin and enolase was observed in the sera of dogs with cod allergy. Among the 36 dogs with IgE reactivity to crude cod extracts, 9 (25%), 14 (39%), and 18 (50%) dogs had specific IgE to parvalbumin, collagen, and tropomyosin, respectively.Conclusions: The dogs exhibited IgE reactivity to the cod allergens which observed in humans, providing support for the use of atopic dogs with fish allergy as a model for fish allergy in humans.

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IgE reactivity to components of fish allergens in Pacific cod (Gadus macrocephalus) in atopic dogs

10.21203/rs.2.19058/v1 ◽

2019 ◽

Author(s):

Ichiro Imanishi ◽

Jumpei Uchiyama ◽

Keijiro Mizukami ◽

Junichi Kamiie ◽

Keigo Kurata ◽

...

Keyword(s):

Specific Ige ◽

Enzyme Linked Immunosorbent Assay ◽

Pacific Cod ◽

Fish Allergy ◽

Ige Reactivity ◽

Allergen Components ◽

Pacific Cod Gadus Macrocephalus ◽

Chromatography Tandem Mass Spectrometry ◽

Gadus Macrocephalus ◽

Liquid Chromatography Tandem Mass

Abstract Background IgE reactivity to fish allergens in atopic dogs, which are used as models for food allergy, has not been elucidated to date. We investigated IgE reactivity to crude and purified Pacific cod (Gadus macrocephalus) allergens in atopic dogs to identify the allergenic components of cod. Methods Specific IgE to crude cod allergens in the sera of 179 atopic dogs, including 27 dogs with cod allergy, were measured using enzyme-linked immunosorbent assay (ELISA). The allergenic components of crude cod antigen were analyzed by ELISA, immunoblotting, and liquid chromatography-tandem mass spectrometry (LC-MS/MS). IgE reactivity to parvalbumin, collagen, and tropomyosin was evaluated using the sera of atopic dogs that were positive for specific IgE to crude cod allergens. Results Specific IgE to crude cod allergens were present in 36 (20%) of the 179 atopic dogs and 12 (44%) of the 27 dogs with cod allergy. In allergen component analysis, IgE reactivity to tropomyosin and enolase was observed in the sera of dogs with cod allergy. Among the 36 dogs with IgE reactivity to crude cod extracts, 9 (25%), 14 (39%), and 18 (50%) dogs had specific IgE to parvalbumin, collagen, and tropomyosin, respectively. Conclusions The dogs exhibited IgE reactivity to the cod allergen components similar to that observed in humans, providing support for the use of atopic dogs with fish allergy as a model for fish allergy in humans.

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