scholarly journals Long-term, in toto live imaging of the developing mouse heart

2020 ◽  
Author(s):  
Yanzhu Yue ◽  
Xin Li ◽  
Youdong Zhang ◽  
Aibin He
Keyword(s):  
Imaging System ◽  
Light Sheet ◽  
Live Imaging ◽  
Mouse Heart ◽  
Cell Behaviors ◽  
Oriented Cell Division ◽  
Lower Vertebrates ◽  
Imaging Strategy ◽  
Cell Intercalation ◽  
Gated Imaging

Abstract Mapping holistic cell behaviors sculpting mammalian heart has been a goal, but so far only successes in transparent invertebrates and lower vertebrates. Using a live-imaging system comprising a customized vertical light-sheet microscope equipped with a culture module, a heartbeat-gated imaging strategy, and a digital image processing framework, we realized imaging of developing mouse hearts with uninterrupted cell lineages for up to 1.5 days. Four-dimensional landscapes of cell behaviors revealed a blueprint for ventricle chamber formation in which biased outward migration of outermost cardiomyocytes coupled with cell intercalation and horizontal division. The trabeculae, an inner muscle architecture, was developed through early fate segregation and transmural cell arrangement involving both oriented cell division and directional migration. Thus, live-imaging reconstruction affords a transformative means for deciphering mammalian organogenesis.

scholarly journals Effect of captopril on post-infarction remodelling visualized by light sheet microscopy and echocardiography

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Urmas Roostalu ◽  
Louise Thisted ◽  
Jacob Lercke Skytte ◽  
Casper Gravesen Salinas ◽  
Philip Juhl Pedersen ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Light Sheet ◽  
Automated Image Analysis ◽  
Border Zone ◽  
Vascular Density ◽  
Mouse Heart ◽  
Imaging Method ◽  
Light Sheet Microscopy ◽  
Post Surgery ◽  
Captopril Treatment

AbstractAngiotensin converting enzyme inhibitors, among them captopril, improve survival following myocardial infarction (MI). The mechanisms of captopril action remain inadequately understood due to its diverse effects on multiple signalling pathways at different time periods following MI. Here we aimed to establish the role of captopril in late-stage post-MI remodelling. Left anterior descending artery (LAD) ligation or sham surgery was carried out in male C57BL/6J mice. Seven days post-surgery LAD ligated mice were allocated to daily vehicle or captopril treatment continued over four weeks. To provide comprehensive characterization of the changes in mouse heart following MI a 3D light sheet imaging method was established together with automated image analysis workflow. The combination of echocardiography and light sheet imaging enabled to assess cardiac function and the underlying morphological changes. We show that delayed captopril treatment does not affect infarct size but prevents left ventricle dilation and hypertrophy, resulting in improved ejection fraction. Quantification of lectin perfused blood vessels showed improved vascular density in the infarct border zone in captopril treated mice in comparison to vehicle dosed control mice. These results validate the applicability of combined echocardiographic and light sheet assessment of drug mode of action in preclinical cardiovascular research.

scholarly journals High-throughput live imaging using Light Sheet Microscopy

2020 ◽  
Author(s):  
Emilio J. Gualda ◽  
Matteo Bernardello ◽  
Maria Marsal ◽  
Pablo Loza Alvarez
Keyword(s):  
High Throughput ◽  
Light Sheet ◽  
Live Imaging ◽  
Light Sheet Microscopy

scholarly journals 3D Tissue elongation via ECM stiffness-cued junctional remodeling

2018 ◽  
Author(s):  
Dong-Yuan Chen ◽  
Justin Crest ◽  
Sebastian J. Streichan ◽  
David Bilder
Keyword(s):  
Basement Membrane ◽  
Cell Shape ◽  
Cell Dynamics ◽  
Src Tyrosine Kinase ◽  
Average Cell ◽  
Cellular Behavior ◽  
Cell Behaviors ◽  
Oriented Cell Division ◽  
Morphometric Analyses ◽  
Membrane Stiffness

ABSTRACTOrgans are sculpted by extracellular as well as cell-intrinsic forces, but how collective cell dynamics are orchestrated in response to microenvironmental cues is poorly understood. Here we apply advanced image analysis to reveal ECM-responsive cell behaviors that drive elongation of the Drosophila follicle, a model 3D system in which basement membrane stiffness instructs tissue morphogenesis. Through in toto morphometric analyses of WT and ‘round egg’ mutants, we find that neither changes in average cell shape nor oriented cell division are required for appropriate organ shape. Instead, a major element is a reorientation of elongated cells at the follicle anterior. Polarized reorientation is regulated by mechanical cues from the basement membrane, which are transduced by the Src tyrosine kinase to alter junctional E-cadherin trafficking. This mechanosensitive cellular behavior represents a conserved mechanism that can elongate ‘edgeless’ tubular epithelia in a process distinct from those that elongate bounded, planar epithelia.

Online Optimization on Operation Parameters of Range-Gated Imaging System Based on Gaussian Process Regression

2019 ◽  
Vol 39 (4) ◽  
pp. 0412010
Author(s):  
程虎 Cheng Hu ◽  
李微 Li Wei ◽  
郭文平 Guo Wenping ◽  
杨克成 Yang Kecheng
Keyword(s):  
Gaussian Process ◽  
Imaging System ◽  
Gaussian Process Regression ◽  
Online Optimization ◽  
Operation Parameters ◽  
Gated Imaging

Study of the Mode and Maximum Detecting Performance of Underwater Laser Range-Gated Imaging System

2011 ◽  
Vol 38 (1) ◽  
pp. 0109001
Author(s):  
韩宏伟 Han Hongwei ◽  
张晓晖 Zhang Xiaohui ◽  
葛卫龙 Ge Weilong
Keyword(s):  
Imaging System ◽  
Laser Range ◽  
Underwater Laser ◽  
Gated Imaging

Multi-resolution MCS auto focus method in range-gated imaging system for underwater

2016 ◽  
Vol 45 (7) ◽  
pp. 0726003
Author(s):  
游瑞蓉 You Ruirong ◽  
王新伟 Wang Xinwei ◽  
周 燕 Zhou Yan
Keyword(s):  
Imaging System ◽  
Auto Focus ◽  
Gated Imaging

scholarly journals Scattering noise estimation of range-gated imaging system in turbid condition

2010 ◽  
Vol 18 (20) ◽  
pp. 21147 ◽  
Author(s):  
ChingSeong Tan ◽  
Gerald Seet ◽  
Andrzej Sluzek ◽  
Xin Wang ◽  
Chai Tong Yuen ◽  
...  
Keyword(s):  
Imaging System ◽  
Noise Estimation ◽  
Gated Imaging ◽  
Scattering Noise

Three-Dimensional Live Imaging of Filamentous Fungi with Light Sheet-Based Fluorescence Microscopy (LSFM)

2017 ◽  
pp. 19-31 ◽  
Author(s):  
Francesco Pampaloni ◽  
Laura Knuppertz ◽  
Andrea Hamann ◽  
Heinz D. Osiewacz ◽  
Ernst H. K. Stelzer
Keyword(s):  
Fluorescence Microscopy ◽  
Filamentous Fungi ◽  
Three Dimensional ◽  
Light Sheet ◽  
Live Imaging

scholarly journals Digital Spindle: A New Way to Explore Mitotic Functions by Whole Cell Data Collection and a Computational Approach

Cells ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 1255
Author(s):  
Norio Yamashita ◽  
Masahiko Morita ◽  
Hideo Yokota ◽  
Yuko Mimori-Kiyosue
Keyword(s):  
Cell Biology ◽  
Information Science ◽  
Three Dimensional ◽  
Light Sheet ◽  
Live Imaging ◽  
Three Dimensions ◽  
Complex Data ◽  
Spatiotemporal Resolution ◽  
Whole Cell ◽  
Light Sheet Microscopy

From cells to organisms, every living system is three-dimensional (3D), but the performance of fluorescence microscopy has been largely limited when attempting to obtain an overview of systems’ dynamic processes in three dimensions. Recently, advanced light-sheet illumination technologies, allowing drastic improvement in spatial discrimination, volumetric imaging times, and phototoxicity/photobleaching, have been making live imaging to collect precise and reliable 3D information increasingly feasible. In particular, lattice light-sheet microscopy (LLSM), using an ultrathin light-sheet, enables whole-cell 3D live imaging of cellular processes, including mitosis, at unprecedented spatiotemporal resolution for extended periods of time. This technology produces immense and complex data, including a significant amount of information, raising new challenges for big image data analysis and new possibilities for data utilization. Once the data are digitally archived in a computer, the data can be reused for various purposes by anyone at any time. Such an information science approach has the potential to revolutionize the use of bioimage data, and provides an alternative method for cell biology research in a data-driven manner. In this article, we introduce examples of analyzing digital mitotic spindles and discuss future perspectives in cell biology.

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