Functional analysis of SlERF01 gene in the disease resistance to S.lycopersici

Abstract Background: Tomato gray leaf spot caused by Stemphylium lycopersici (S. lycopersici) is a serious disease that can severely hinder tomato production. To date, only Sm has been reported to provide resistance against this disease, and the molecular mechanism underlying resistance to this disease in tomato remains unclear. To better understand the mechanism of tomato resistance to S. lycopersici, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR)-based analysis, physiological indexes, microscopy observations and transgenic technology were used in this study.Results: Our results showed that the expression of SlERF01 was strongly induced by S. lycopersici and by exogenous applications of the hormones salicylic acid (SA) and jasmonic acid (JA). Furthermore, overexpression of SlERF01 enhanced the hypersensitive response (HR) to S. lycopersici and elevated the expression of defense genes in tomato. Furthermore, the accumulation of lignin, callose and hydrogen peroxide (H2O2) increased in the transgenic lines after inoculation with S. lycopersici. Taken together, our results showed that SlERF01 played an indispensable role in multiple SA, JA and reactive oxygen species (ROS) signaling pathways to provide resistance to S. lycopersici invasion. Our findings also indicated that SlERF01 could activate the expression of the PR1 gene and enhance resistance to S. lycopersici.Conclusions: We identified the SlERF01 gene, which encodes a novel tomato AP2/ERF transcription factor (TF). Functional analysis revealed that SlERF01 positively regulates tomato resistance to S. lycopersici. Our findings indicate that SlERF01 plays a key role in multiple SA, JA and ROS signaling pathways to provide resistance to invasion by S. lycopersici. The findings of this study not only help to better understand the mechanisms of response to pathogens but also enable targeted breeding strategies for tomato resistance to S. lycopersici.

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Functional analysis of the SlERF01 gene in disease resistance to S. lycopersici

10.21203/rs.2.24362/v5 ◽

2020 ◽

Author(s):

Huanhuan Yang ◽

Fengyi Shen ◽

Hexuan Wang ◽

Tingting Zhao ◽

He Zhang ◽

...

Keyword(s):

Functional Analysis ◽

Signaling Pathways ◽

Gray Leaf Spot ◽

Tomato Production ◽

Ros Signaling ◽

Transgenic Lines ◽

Physiological Indexes ◽

Tomato Gray Leaf Spot ◽

Serious Disease ◽

Resistance To Invasion

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Functional analysis of SlERF01 gene in the disease resistance to S.lycopersici

10.21203/rs.2.24362/v1 ◽

2020 ◽

Author(s):

Huanhuan Yang ◽

Jingfu Li

Keyword(s):

Signaling Pathways ◽

Gray Leaf Spot ◽

Pcr Analysis ◽

Functional Verification ◽

Leaf Spot Disease ◽

Tomato Production ◽

Ros Signaling ◽

Transgenic Lines ◽

Serious Disease ◽

Resistance To Invasion

Abstract Background: Tomato gray leaf spot disease caused by Stemphylium lycopersici (S. lycopersici) is a serious disease that can severely affect tomato production. So far, only resistance gene Sm has been reported and the molecular mechanism of tomato resistance to the disease remains unclear. To better understand this mechanism of tomato resistance to S. lycopersici, qRT-PCR analysis, physiological index determination, microscopic observation and transgenic technology were used in this study.Results: Our results showed that SlERF01 could be strongly induced by S. lycopersici and the exogenous hormones salicylic acid (SA) and jasmonic acid (JA). Furthermore, overexpression of SlERF01 enhanced the hypersensitive response to S. lycopersici and elevated the expression of defense genes in tomato. Furthermore, the accumulation of lignin, callose and H2O2 was increased in transgenic lines after inoculation with S. lycopersici. Here, our results showed that SlERF01 played an indispensable role in among multiple SA, JA and ROS signaling pathways to confer resistance to S. lycopersici invasion. Our findings also indicated that SlERF01 could activate PR1 gene expression and enhance resistance to S. lycopersici.Conclusions: We identified SlERF01, a novel tomato AP2/ERF transcription factor. The Functional verification demonstrated SlERF01 positively regulated tomato resistance to S. lycopersici. Our findings indicate that SlERF01 plays a key role in the multiple SA, JA and ROS signaling pathways to confer resistance to invasion by S. lycopersici. The findings of this study not only can help to better understanding of mechanisms of response to pathogens, but also will enable targeted breeding strategies for tomato resistance to S. lycopersici.

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Functional analysis of SlERF01 gene in the disease resistance to S.lycopersici

10.21203/rs.2.24362/v3 ◽

2020 ◽

Author(s):

Huanhuan Yang ◽

Fengyi Shen ◽

Hexuan Wang ◽

Tingting Zhao ◽

He Zhang ◽

...

Keyword(s):

Signaling Pathways ◽

Gray Leaf Spot ◽

Pcr Analysis ◽

Functional Verification ◽

Leaf Spot Disease ◽

Tomato Production ◽

Ros Signaling ◽

Study Results ◽

Transgenic Lines ◽

Serious Disease

Abstract Background: Tomato gray leaf spot disease caused by Stemphylium lycopersici ( S. lycopersici ) is a serious disease that can severely affect tomato production. So far, only resistance gene Sm has been reported and the molecular mechanism of tomato resistance to the disease remains unclear. To better understand this mechanism of tomato resistance to S. lycopersici , qRT-PCR analysis, physiological index determination, microscopic observation and transgenic technology were used in this study. Results: Our results showed that SlERF01 could be strongly induced by S. lycopersici and the exogenous hormones salicylic acid (SA) and jasmonic acid (JA). Furthermore, overexpression of SlERF01 enhanced the hypersensitive response to S. lycopersici and elevated the expression of defense genes in tomato. Furthermore, the accumulation of lignin, callose and H 2 O 2 was increased in transgenic lines after inoculation with S. lycopersici. Here, our results showed that SlERF01 played an indispensable role in among multiple SA, JA and ROS signaling pathways to confer resistance to S. lycopersici invasion. Our findings also indicated that SlERF01 could activate PR1 gene expression and enhance resistance to S. lycopersici . Conclusions: We identified SlERF01 , a novel tomato AP2/ERF transcription factor. The Functional verification demonstrated SlERF01 positively regulated tomato resistance to S. lycopersici . Our findings indicate that SlERF01 plays a key role in the multiple SA, JA and ROS signaling pathways to confer resistance to invasion by S. lycopersici. The findings of this study not only can help to better understanding of mechanisms of response to pathogens, but also will enable targeted breeding strategies for tomato resistance to S. lycopersici .

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Functional analysis of SlERF01 gene in the disease resistance to S.lycopersici

10.21203/rs.2.24362/v2 ◽

2020 ◽

Author(s):

Huanhuan Yang ◽

Fengyi Shen ◽

Hexuan Wang ◽

Tingting Zhao ◽

He Zhang ◽

...

Keyword(s):

Signaling Pathways ◽

Gray Leaf Spot ◽

Pcr Analysis ◽

Functional Verification ◽

Leaf Spot Disease ◽

Tomato Production ◽

Ros Signaling ◽

Study Results ◽

Transgenic Lines ◽

Serious Disease

Abstract Background: Tomato gray leaf spot disease caused by Stemphylium lycopersici ( S. lycopersici ) is a serious disease that can severely affect tomato production. So far, only resistance gene Sm has been reported and the molecular mechanism of tomato resistance to the disease remains unclear. To better understand this mechanism of tomato resistance to S. lycopersici , qRT-PCR analysis, physiological index determination, microscopic observation and transgenic technology were used in this study. Results: Our results showed that SlERF01 could be strongly induced by S. lycopersici and the exogenous hormones salicylic acid (SA) and jasmonic acid (JA). Furthermore, overexpression of SlERF01 enhanced the hypersensitive response to S. lycopersici and elevated the expression of defense genes in tomato. Furthermore, the accumulation of lignin, callose and H 2 O 2 was increased in transgenic lines after inoculation with S. lycopersici. Here, our results showed that SlERF01 played an indispensable role in among multiple SA, JA and ROS signaling pathways to confer resistance to S. lycopersici invasion. Our findings also indicated that SlERF01 could activate PR1 gene expression and enhance resistance to S. lycopersici . Conclusions: We identified SlERF01 , a novel tomato AP2/ERF transcription factor. The Functional verification demonstrated SlERF01 positively regulated tomato resistance to S. lycopersici . Our findings indicate that SlERF01 plays a key role in the multiple SA, JA and ROS signaling pathways to confer resistance to invasion by S. lycopersici. The findings of this study not only can help to better understanding of mechanisms of response to pathogens, but also will enable targeted breeding strategies for tomato resistance to S. lycopersici .

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A functional analysis to dissect the role of ROS signaling pathways using chemogenetic tools and genetically encoded biosensors for in vitro studies

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2020.12.291 ◽

2021 ◽

Vol 165 ◽

pp. 17

Author(s):

Yusuf Ceyhun Erdoğan ◽

Serap Sezen ◽

Büşra Nur Ata ◽

Zeynep Çokluk ◽

Melike Seçilmiş ◽

...

Keyword(s):

Functional Analysis ◽

Signaling Pathways ◽

In Vitro Studies ◽

Ros Signaling

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Faculty Opinions recommendation of Integrating Omics data for signaling pathways, interactome reconstruction, and functional analysis.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.10631956.11516054 ◽

2011 ◽

Author(s):

Marylyn Ritchie ◽

Stephen Turner

Keyword(s):

Functional Analysis ◽

Signaling Pathways ◽

Omics Data

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cROStalk for Life: Uncovering ROS Signaling in Plants and Animal Systems, from Gametogenesis to Early Embryonic Development

Genes ◽

10.3390/genes12040525 ◽

2021 ◽

Vol 12 (4) ◽

pp. 525

Author(s):

Valentina Lodde ◽

Piero Morandini ◽

Alex Costa ◽

Irene Murgia ◽

Ignacio Ezquer

Keyword(s):

Signal Transduction ◽

Signaling Pathways ◽

Analytical Techniques ◽

Antioxidant Systems ◽

Developmental Studies ◽

Science Field ◽

Ros Signaling ◽

Reproductive Processes

This review explores the role of reactive oxygen species (ROS)/Ca2+ in communication within reproductive structures in plants and animals. Many concepts have been described during the last years regarding how biosynthesis, generation products, antioxidant systems, and signal transduction involve ROS signaling, as well as its possible link with developmental processes and response to biotic and abiotic stresses. In this review, we first addressed classic key concepts in ROS and Ca2+ signaling in plants, both at the subcellular, cellular, and organ level. In the plant science field, during the last decades, new techniques have facilitated the in vivo monitoring of ROS signaling cascades. We will describe these powerful techniques in plants and compare them to those existing in animals. Development of new analytical techniques will facilitate the understanding of ROS signaling and their signal transduction pathways in plants and mammals. Many among those signaling pathways already have been studied in animals; therefore, a specific effort should be made to integrate this knowledge into plant biology. We here discuss examples of how changes in the ROS and Ca2+ signaling pathways can affect differentiation processes in plants, focusing specifically on reproductive processes where the ROS and Ca2+ signaling pathways influence the gametophyte functioning, sexual reproduction, and embryo formation in plants and animals. The study field regarding the role of ROS and Ca2+ in signal transduction is evolving continuously, which is why we reviewed the recent literature and propose here the potential targets affecting ROS in reproductive processes. We discuss the opportunities to integrate comparative developmental studies and experimental approaches into studies on the role of ROS/ Ca2+ in both plant and animal developmental biology studies, to further elucidate these crucial signaling pathways.

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