Halomonas Salinarum sp. nov., a Moderately Halophilic Bacterium, Isolated from Saline Soil in Yingkou, China

2021 ◽  
Author(s):  
Ya-Lin Yin ◽  
Fang-Ling Li ◽  
Lei Wang
Keyword(s):  
Saline Soil ◽  
Halophilic Bacterium ◽  
Phylogenetic Position ◽  
Hypersaline Environments ◽  
Respiratory Quinone ◽  
16S Rrna Analysis ◽  
Gram Staining ◽  
A Value ◽  
Moderately Halophilic Bacterium ◽  
Major Respiratory Quinone

Abstract Strains of Halomonas, thought to play vital roles in the environment for their versatility, are ubiquitous in hypersaline environments. A Gram-staining-negative, moderately halotolerant, facultatively aerobic, motile bacterium, designated G5-11T, was isolated from saline soil in Yingkou of Liaoning, China. The cells of strain G5-11T grew at 4-35 ℃ (optimum 30 ℃), at pH 6.0-9.0 (optimum 8.0) and in the presense of 3-15 % (w/v) NaCl (optimum 5 %). The strain could be clearly distinguished from the related species of the genus Halomonas by its phylogenetic position and biochemical characteristics. It presented Q-9 as the major respiratory quinone and the dominant cellular fatty acids were summed feature 8 (C18:1 ω7c/ C18:1 ω6c), C16:0 and summed feature 3 (C16:1 ω7c/ C16:1 ω6c). The polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol as the major components. The G+C content of strain G5-11T genome was 61.0 mol%. 16S rRNA analysis showed that strain G5-11T had the highest similarity to Halomonas niordiana LMG 31227T and Halomonas taeanensis DSM 16463T, both reaching 98.3 %, followed by Halomonas pacifica NBRC 102220T with a value of 95.8 %. Based on phenotypic, chemotaxonomic and phylogenetic inferences, strain G5-11T represents a novel species of the genus Halomonas, for which the name Halomonas salinarum sp. nov. is proposed. The type strain of Halomonas salinarum is G5-11T (=CGMCC 1.12051T=LMG 31677T).

scholarly journals Halomonas Maris Sp. Nov., a Moderately Halophilic Bacterium Isolated From Marine Sediment in the Southwest Indian Ocean

2021 ◽  
Author(s):  
Xu Qiu ◽  
Xiaorong Cao ◽  
Guangxin Xu ◽  
Huangming Wu ◽  
Xixiang Tang
Keyword(s):  
Indian Ocean ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Chromosomal Dna ◽  
Unidentified Phospholipid ◽  
Respiratory Quinone ◽  
Southwest Indian Ocean ◽  
Deep Sea Sediment ◽  
Gram Staining ◽  
Moderately Halophilic Bacterium

Abstract A halophilic, Gram-staining-negative, rod-shaped, flagellated and motile bacterium, strain QX-1T, was isolated from deep-sea sediment at a depth of 3332 m in the southwestern Indian Ocean. Strain QX-1T growth was observed at 4–50 °C (optimum 37 °C), pH 5.0–11.0 (optimum pH 7.0), 3%–25% NaCl (w/v; optimum 7%), and it did not grow without NaCl. A phylogenetic analysis based on the 16S rRNA gene placed strain QX-1T in the genus Halomonas and most closely related to Halomonas sulfidaeris (97.90%), Halomonas zhaodongensis (97.80%), Halomonas songnenensis (97.59%), Halomonas hydrothermalis (97.37%), Halomonas subterranea (97.25%), Halomonas salicampi (97.09%), and Halomonas arcis (97.01%). DNA–DNA hybridization (< 26.50%) and average nucleotide identity values (< 83.54%) between strain QX-1T and the related type strains meet the accepted criteria for a new species. The principal fatty acids (> 10%) of strain QX-1T are C16:0 (25.50%), C17:0 cyclo (14.02%), C19:0 cyclo ω8c (18.72%), and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c, 18.08%). The polar lipids of strain QX-1T are mainly diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, unidentified phospholipid, unidentified aminophospholipid, and five unidentified lipids. The main respiratory quinone is Q-9. The G+C content of its chromosomal DNA is 54.4 mol%. Its fatty acid profile, respiratory quinones, and G+C content also support the placement of QX-1T in the genus Halomonas. These phylogenetic, phenotypic, and chemotaxonomic analyses indicate that QX-1T is a novel species, for which the name Halomonas maris is proposed. The type strain is QX-1T (=MCCC 1A17875T = KCTC 82198T = NBRC 114670T).

scholarly journals Hwanghaeicola aestuarii gen. nov., sp. nov., a moderately halophilic bacterium isolated from a tidal flat of the Yellow Sea

2010 ◽  
Vol 60 (12) ◽  
pp. 2877-2881 ◽  
Author(s):  
Jeong Myeong Kim ◽  
Ji Young Jung ◽  
Ho Byoung Chae ◽  
Woojun Park ◽  
Che Ok Jeon
Keyword(s):  
Tidal Flat ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
The Yellow Sea ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Moderately Halophilic ◽  
Gram Staining ◽  
Moderately Halophilic Bacterium

A moderately halophilic Gram-staining-negative bacterium, designated strain Y26T, was isolated from a tidal flat of Taean coast in South Korea. Cells were strictly aerobic, motile cocci with a single flagellum and showed catalase- and oxidase-positive reactions. Growth of strain Y26T was observed at 15–35 °C (optimum 25–30 °C), pH 6.0–8.0 (optimum pH 6.5–7.5) and with 1.5–6.0 % (w/v) NaCl (optimum 2.0–3.0 %). The predominant fatty acids were C18 : 1 ω7c (66.2 %), C16 : 0 (12.4 %) and C10 : 0 3-OH (5.0 %) and the G+C content of the genomic DNA was 61.0 mol%. Strain Y26T contained ubiquinone-10 (Q-10) as the major respiratory quinone. Comparative 16S rRNA gene sequence analysis showed that strain Y26T formed a distinct phyletic lineage from other genera within the Roseobacter clade of the class Alphaproteobacteria and was most closely related to members of the genera Maribius, Maritimibacter and Palleronia with 93.8–94.6 % sequence similarity. On the basis of chemotaxonomic data and molecular properties, strain Y26T represents a novel genus, Hwanghaeicola, within the family Rhodobacteraceae, for which the name Hwanghaeicola aestuarii gen. nov., sp. nov. is proposed. The type strain is Y26T (=KACC 13705T =DSM 22009T).

Paraliobacillus salinarum sp. nov., isolated from saline soil in Yingkou, China

2021 ◽  
Vol 71 (7) ◽  
Author(s):  
Ya-Lin Yin ◽  
Fang-Ling Li ◽  
Xian-Yuan Du ◽  
Ya-Xi Zhang ◽  
Lei Wang
Keyword(s):  
Dna Hybridization ◽  
Type Species ◽  
Saline Soil ◽  
Phylogenetic Position ◽  
16S Rrna Analysis ◽  
Content Type ◽  
Link Type ◽  
A Value ◽  
Optimum Ph ◽  
Pr China

A novel Gram-stain-positive, facultatively aerobic, slightly halophilic, endospore-forming bacterium, designated G6-18T, was isolated from saline soil collected in Yingkou, Liaoning, PR China. Cells of strain G6-18T grew at 10–37 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 8.0) and in the presence of 2–15 % (w/v) NaCl (optimum, 5 %). The strain could be clearly distinguished from the related species of the genus Paraliobacillus by its phylogenetic position and biochemical characteristics. It presented MK-7 as the major quinone and the dominant cellular fatty acids were iso-C16 : 0, anteiso-C15 : 0, C16 : 0 and iso-C14 : 0. The polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol as the major components. The G+C content of strain G6-18T genome was 35.3 mol%. 16S rRNA analysis showed that strain G6-18T had the highest similarity to Paraliobacillus ryukyuensis DSM 15140T, reaching 97.0 %, followed by Paraliobacillus quinghaiensis CGMCC 1.6333T with a value of 96.3 %. The average nucleotide identity values between strain G6-18T and Paraliobacillus ryukyuensis DSM 15140T, Paraliobacillus sedimins KCTC 33762T, Paraliobacillus quinghaiensis CGMCC 1.6333T and Paraliobacillus zengyii DSM 107811T were 74.3, 72.0, 73.2 and 72.8 %, respectively, and the digital DNA–DNA hybridization values between strain G6-18T and the neighbouring strains were 15.6, 13.8, 14.2 and 14.2 %, respectively. Based on phenotypic, chemotaxonomic and phylogenetic inferences, strain G6-18T represents a novel species of the genus Paraliobacillus , for which the name Paraliobacillus salinarum sp. nov. (=CGMCC 1.12058T=DSM 25428T) is proposed.

scholarly journals Streptohalobacillus salinus gen. nov., sp. nov., a moderately halophilic, Gram-positive, facultative anaerobe isolated from subsurface saline soil

2011 ◽  
Vol 61 (5) ◽  
pp. 1127-1132 ◽  
Author(s):  
Xiaowei Wang ◽  
Yanfen Xue ◽  
Yanhe Ma
Keyword(s):  
Saline Soil ◽  
Qaidam Basin ◽  
Halophilic Bacterium ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium ◽  
The Family ◽  
Diamino Acid

A Gram-stain-positive, rod-shaped, non-sporulating, motile and moderately halophilic bacterium, designated strain H96B60T, was isolated from a saline soil sample of the Qaidam basin, China. The strain was facultatively anaerobic. Major end products formed from glucose fermentation were acetate, ethanol and lactic acid. The cell-wall peptidoglycan contained meso-diaminopimelic acid as the diagnostic diamino acid. The isoprenoid quinone component was menaquinone-6 (MK-6). The predominant cellular fatty acids were C16 : 0, anteiso-C13 : 0 and anteiso-C15 : 0. The genomic DNA G+C content of strain H96B60T was 36.2 mol%. Phylogenetic analysis based on comparative 16S rRNA gene sequences indicated that strain H96B60T represented a novel phyletic lineage within the family Bacillaceae and was related most closely to Halolactibacillus species (96.1–96.4 % similarity). Based on the phenotypic, chemotaxonomic and phylogenetic data presented, strain H96B60T is considered to represent a novel species of a new genus, for which the name Streptohalobacillus salinus gen. nov., sp. nov. is proposed. The type strain of Streptohalobacillus salinus is H96B60T ( = DSM 22440T  = CGMCC 1.7733T).

scholarly journals Marinobacter gudaonensis sp. nov., isolated from an oil-polluted saline soil in a Chinese oilfield

2007 ◽  
Vol 57 (2) ◽  
pp. 250-254 ◽  
Author(s):  
Jun Gu ◽  
Hua Cai ◽  
Su-Lin Yu ◽  
Ri Qu ◽  
Bin Yin ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Saline Soil ◽  
Sequence Similarity ◽  
Eastern China ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Shengli Oilfield ◽  
Major Respiratory Quinone ◽  
Reference Strains

Two novel strains, SL014B61AT and SL014B11A, were isolated from an oil-polluted saline soil from Gudao in the coastal Shengli Oilfield, eastern China. Cells of strains SL014B61AT and SL014B11A were motile, Gram-negative and rod-shaped. Growth occurred at NaCl concentrations of between 0 and 15 % and at temperatures of between 10 and 45 °C. Strain SL014B61AT had Q9 as the major respiratory quinone and C16 : 0 (21.2 %), C18 : 1ω9c (20.3 %), C16 : 1ω7c (7.3 %) and C16 : 1ω9c (6.4 %) as predominant fatty acids. The G+C content of the DNA was 57.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SL014B61AT belonged to the genus Marinobacter in the class Gammaproteobacteria. Strain SL014B61AT showed the highest 16S rRNA gene sequence similarity with Marinobacter bryozoorum (97.9 %) and showed 97.8 % sequence similarity to Marinobacter lipolyticus. DNA–DNA relatedness to the reference strains Marinobacter bryozoorum and Marinobacter lipolyticus was 35.5 % and 33.8 %, respectively. On the basis of these data, it is proposed that strains SL014B61AT and SL014B11A represent a novel species, Marinobacter gudaonensis sp. nov. The type strain is strain SL014B61AT (=DSM 18066T=LMG 23509T=CGMCC 1.6294T).

Flavobacterium chungbukense sp. nov., isolated from soil

2011 ◽  
Vol 61 (11) ◽  
pp. 2734-2739 ◽  
Author(s):  
Chae-Sung Lim ◽  
Yong-Sik Oh ◽  
Jae-Kwan Lee ◽  
A-Rum Park ◽  
Jae-Soo Yoo ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Rrna Gene ◽  
Strictly Aerobic ◽  
Respiratory Quinone ◽  
Gram Staining ◽  
Flavobacterium Johnsoniae ◽  
The Family ◽  
Major Respiratory Quinone ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

A yellow-pigmented, Gram-staining-negative, non-motile, strictly aerobic and rod-shaped bacterium, designated CS100T, was isolated from soil in Chungbuk, Korea. Phylogenetic analysis and comparative studies based on the 16S rRNA gene sequence showed that strain CS100T belonged to the genus Flavobacterium in the family Flavobacteriaceae. Strain CS100T showed the highest sequence similarities to Flavobacterium glaciei JCM 13953T (97.6 %) and Flavobacterium johnsoniae KACC 11410T (97.1 %). Sequence similarity to other members of the genus Flavobacterium was 91.5–97.0 %. Growth occurred at 4–30 °C, at pH 5.0–9.0 and in the presence of 0–2 % (w/v) NaCl. Flexirubin-type pigments were produced. Menaquinone-6 (MK-6) was the major respiratory quinone and the major fatty acids were iso-C15 : 0 (17.3 %), summed feature 3 (comprising iso-C15 : 0 2-OH and/or C16 : 1ω7c, 15.5 %) and C16 : 0 (11.8 %). The DNA G+C content was 36.4 mol%. Strain CS100T hydrolysed skimmed milk and gelatin, but not chitin or pectin, and showed oxidase and catalase activities. DNA–DNA relatedness was 3.0 % with F. glaciei JCM 13953T and 11.5 % with F. johnsoniae KACC 11410T. On the basis of the evidence from this study, strain CS100T represents a novel species of the genus Flavobacterium, for which the name Flavobacterium chungbukense sp. nov. is proposed. The type strain is CS100T ( = KACC 15048T = JCM 17386T).

scholarly journals Flavobacterium palustre sp. nov., isolated from wetland soil

2015 ◽  
Vol 65 (Pt_3) ◽  
pp. 1003-1007 ◽  
Author(s):  
Hao Feng ◽  
Yanhua Zeng ◽  
Yili Huang
Keyword(s):  
Type Species ◽  
Polar Lipids ◽  
Wetland Soil ◽  
Rrna Gene ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type ◽  
Gram Staining ◽  
Major Respiratory Quinone ◽  
Xixi Wetland

A Gram-staining-negative, non-motile, yellow-coloured, rod-shaped bacterium, designated S44T, was isolated from bankside soil of Xixi wetland, located in Zhejiang province, China. Growth of strain S44T was observed at 6–37 °C (optimum, 28 °C) and at pH 6.0–9.0 (optimum, 7.0). No growth occurred in the presence of >2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain S44T represented a member of the genus Flavobacterium , showing the highest sequence similarities to the sequences from Flavobacterium succinicans DSM 4002T (96.9 %), Flavobacterium reichenbachii WB 3.2-61T (96.6 %) and Flavobacterium glycines NCBI 105008T (96.5 %). The G+C content of the genomic DNA was 33.6 mol%. The predominant cellular fatty acids were C15 : 0, iso-C15 : 0, anteiso-C15 : 0 and summed feature 3 (comprising C16 : 1ω7c and/or iso-C15 : 0 2-OH), and the major respiratory quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unknown aminolipids, two unknown aminophospholipids and four unknown polar lipids. On the basis of the phenotypic and genotypic data, it is proposed that the isolate S44T be classified as representing a novel species of the genus Flavobacterium , for which the name Flavobacterium palustre sp. nov. is proposed. The type strain is S44T ( = CGMCC 1.12811T = NBRC 110389T).

Virgibacillus litoralis sp. nov., a moderately halophilic bacterium isolated from saline soil

2009 ◽  
Vol 96 (3) ◽  
pp. 323-329 ◽  
Author(s):  
Yi-Guang Chen ◽  
Zhu-Xiang Liu ◽  
De-Jiao Peng ◽  
Yu-Qin Zhang ◽  
Yong-Xia Wang ◽  
...  
Keyword(s):  
Saline Soil ◽  
Halophilic Bacterium ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium

Salinicoccus salitudinis sp. nov., a new moderately halophilic bacterium isolated from a saline soil sample

Extremophiles ◽  
2007 ◽  
Vol 12 (2) ◽  
pp. 197-203 ◽  
Author(s):  
Yi-Guang Chen ◽  
Xiao-Long Cui ◽  
Wen-Jun Li ◽  
Li-Hua Xu ◽  
Meng-Liang Wen ◽  
...  
Keyword(s):  
Soil Sample ◽  
Saline Soil ◽  
Halophilic Bacterium ◽  
Moderately Halophilic ◽  
Moderately Halophilic Bacterium

scholarly journals Marinobacterium lutimaris sp. nov., isolated from a tidal flat

2010 ◽  
Vol 60 (8) ◽  
pp. 1828-1831 ◽  
Author(s):  
Jeong Myeong Kim ◽  
Se Hee Lee ◽  
Ji Young Jung ◽  
Che Ok Jeon
Keyword(s):  
Tidal Flat ◽  
Type Strain ◽  
Novel Species ◽  
Halophilic Bacterium ◽  
Molecular Data ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Gram Staining ◽  
Moderately Halophilic Bacterium ◽  
Optimum Ph

A Gram-staining-negative, moderately halophilic bacterium, designated strain AN9T, was isolated from a tidal flat of the Taean coast in South Korea. Cells were catalase- and oxidase-positive short rods that were motile by means of a single polar flagellum. Growth of strain AN9T was observed at 15–40 °C (optimum, 25–30 °C) and at pH 6.0–8.0 (optimum, pH 6.5–7.5). Strain AN9T contained ubiquinone Q-8 as the predominant isoprenoid quinone and C10 : 0 3-OH (31.7 %), C18 : 1 ω7c (24.8 %), C16 : 0 (14.7 %) and summed feature 3 (comprising C16 : 1 ω7c and/or iso-C15 : 0 2-OH, 10.72 %) as the major fatty acids. The G+C content of the genomic DNA of strain AN9T was 58 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain AN9T was related to members of the genus Marinobacterium and was related most closely to Marinobacterium litorale IMCC1877T (96.8 % similarity). On the basis of chemotaxonomic and molecular data, strain AN9T is considered to represent a novel species of the genus Marinobacterium, for which the name Marinobacterium lutimaris sp. nov. is proposed. The type strain is AN9T (=KACC 13703T =DSM 22012T).

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