scholarly journals Synthesis of tobacco-derived cembratriene-ol and cembratriene-diol in yeast using engineered enzymes

2020 ◽  
Author(s):  
Yu Zhang ◽  
Shiquan Bian ◽  
Xiaofeng Liu ◽  
Ning Fang ◽  
Chunkai Wang ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Sustainable Agriculture ◽  
Biological Activities ◽  
Protein Fusion ◽  
Technical Approach ◽  
Mva Pathway ◽  
Geranyl Diphosphate ◽  
Cytochrome P450 Hydroxylase ◽  
Yeast Strains ◽  
Anti Fungal

Abstract Background: Cembranoids are one kind of diterpenoids with multiple biological activities, and the tobacco cembatriene-ol (CBT-ol) and cembatriene-diol (CBT-diol) have high anti-insect and anti-fungal activities, which is attracting great attention for their potential usage in sustainable agriculture. Cembranoids have been supposed to be formed through the 2-C-methyl-D-erythritol-4- phosphate (MEP) pathway, yet the involvement of mevalonate (MVA) pathway in their synthesis remains unclear. Exploring the roles of MVA pathway in cembranoid synthesis could contribute not only to the technical approach but also to the molecular mechanismfor cembranoid biosynthesis. Results: We constructed a vector to express an engineered protein fusion of cembranol synthase (CBTS1) and the GAL4 AD domain as a N-terminal translation leader. Eventually, the engineered enzyme AD-CTBS1 was successfully expressed, which further resulted in the production of CBT-ol in yeast with an optimized MVA pathway for geranyl-geranyl diphosphate (GGPP) production, but not in other yeast strains with low GGPP supply. Subsequently, CBT-diol was also synthesized by co-expression of engine-ered cembranol synthase (CBTS1) and cytochrome P450 hydroxylase (CYP450) in the yeast enhanced MVA pathway. Conclusions: We demonstrated that yeast could be applied to the production of tobacco-derived CBT-ol and CBT-diol, which are anti-fungal compounds. And, established a new way to produce the tobacco-derived CBT-ol and CBT-diol in yeast with optimized MVA pathway for GGPP production. Thus, this study established a feasibility for cembranoid production via the MVA pathway and provided an alternative bio-approach for the production of cembranoids in microbes.

scholarly journals Synthesis of cembratriene-ol and cembratriene-diol in yeast via the MVA pathway

2021 ◽  
Author(s):  
Yu Zhang ◽  
Shiquan Bian ◽  
Xiaofeng Liu ◽  
Ning Fang ◽  
Chunkai Wang ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Sustainable Agriculture ◽  
Yeast Strain ◽  
Biological Activities ◽  
Geranylgeranyl Diphosphate ◽  
Technical Approach ◽  
Mva Pathway ◽  
Yeast Strains ◽  
Modified Enzyme ◽  
Anti Fungal

Abstract Background: Cembranoids are one kind of diterpenoids with multiple biological activities. The tobacco cembratriene-ol (CBT-ol) and cembratriene-diol (CBT-diol) have high anti-insect and anti-fungal activities, which is attracting great attentions for their potential usage in sustainable agriculture. Cembranoids were supposed to be formed through the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway, yet the involvement of mevalonate (MVA) pathway in their synthesis remains unclear. Exploring the roles of MVA pathway in cembranoid synthesis could contribute not only to the technical approach but also to the molecular mechanism for cembranoid biosynthesis.Results: We constructed vectors to express cembratriene-ol synthase (CBTS1) and its fusion protein (AD-CBTS1) containing an N-terminal GAL4 AD domain as a translation leader in yeast. Eventually, the modified enzyme AD-CBTS1 was successfully expressed, which further resulted in the production of CBT-ol in the yeast strain BY-T20 with enhanced MVA pathway for geranylgeranyl diphosphate (GGPP) production but not in other yeast strains with low GGPP supply. Subsequently, CBT-diol was also synthesized by co-expression of the modified enzyme AD-CBTS1 and BD-CYP450 in the yeast strain BY-T20.Conclusions: We demonstrated that yeast is insensitive to the tobacco anti-fungal compound CBT-ol or CBT-diol and could be applied to their biosynthesis. This study further established a feasibility for cembranoid production via the MVA pathway and provided an alternative bio-approach for cembranoid biosynthesis in microbes.

scholarly journals Synthesis of cembratriene-ol and cembratriene-diol in yeast via the MVA pathway

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Yu Zhang ◽  
Shiquan Bian ◽  
Xiaofeng Liu ◽  
Ning Fang ◽  
Chunkai Wang ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Sustainable Agriculture ◽  
Yeast Strain ◽  
Biological Activities ◽  
Geranylgeranyl Diphosphate ◽  
Technical Approach ◽  
Mva Pathway ◽  
Yeast Strains ◽  
Modified Enzyme ◽  
Anti Fungal

Abstract Background Cembranoids are one kind of diterpenoids with multiple biological activities. The tobacco cembratriene-ol (CBT-ol) and cembratriene-diol (CBT-diol) have high anti-insect and anti-fungal activities, which is attracting great attentions for their potential usage in sustainable agriculture. Cembranoids were supposed to be formed through the 2-C-methyl-d-erythritol-4-phosphate (MEP) pathway, yet the involvement of mevalonate (MVA) pathway in their synthesis remains unclear. Exploring the roles of MVA pathway in cembranoid synthesis could contribute not only to the technical approach but also to the molecular mechanism for cembranoid biosynthesis. Results We constructed vectors to express cembratriene-ol synthase (CBTS1) and its fusion protein (AD-CBTS1) containing an N-terminal GAL4 AD domain as a translation leader in yeast. Eventually, the modified enzyme AD-CBTS1 was successfully expressed, which further resulted in the production of CBT-ol in the yeast strain BY-T20 with enhanced MVA pathway for geranylgeranyl diphosphate (GGPP) production but not in other yeast strains with low GGPP supply. Subsequently, CBT-diol was also synthesized by co-expression of the modified enzyme AD-CBTS1 and BD-CYP450 in the yeast strain BY-T20. Conclusions We demonstrated that yeast is insensitive to the tobacco anti-fungal compound CBT-ol or CBT-diol and could be applied to their biosynthesis. This study further established a feasibility for cembranoid production via the MVA pathway and provided an alternative bio-approach for cembranoid biosynthesis in microbes.

scholarly journals Ethnobotanical, phytochemistry, and pharmacological property of Waltheria Indica Linn

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
C Nirmala ◽  
M Sridevi
Keyword(s):  
Inflammatory Diseases ◽  
Biological Activities ◽  
Perennial Herb ◽  
Main Body ◽  
Biological Targets ◽  
Traditional Uses ◽  
Whole Plant ◽  
Anti Cancer ◽  
Microbial Infections ◽  
Anti Fungal

Abstract Background In modern therapeutics, various human pathological disturbances were treated with the plant-based products. Waltheria indica Linn, a perennial herb, was commonly used in traditional medicine worldwide against various ailments such as cough, dysentery, diarrhea, bladder disorder, hemoptysis, inflammations, neuralgia, wounds, and ulcers. Main body The shrub was majorly distributed in tropical, subtropical regions and exists in many distinct local forms. Both the crude extracts and purified compounds from the whole plant and its parts showed wide pharmacological properties like antioxidant, analgesic, sedative, anti-bacterial, anti-fungal, and anti-parasitic. The phytochemical profile and traditional usage highlight the potency of the plant in the treatment of microbial infections and inflammatory diseases. Yet, additional studies are required for the confirmations of its traditional uses against other diseases. More detailed understanding of anti-cataract, anti-diabetics, asthma, anemia, and anti-cancer mechanism has to be explored. Though many research articles on the proposed plant are available, there has been a rising concern in the therapeutic property, especially on the alkaloids and flavonoids from this plant for drug design. Conclusion This article aims in a systematic and updated review on distribution, botany, traditional uses, phytocompounds, and relevant biological activities from each part of the plant. The information was collected from databases like PubMed, ScienceDirect, Web of Science, Google Scholar, books, dissertation, and reports via academic libraries that included more than 100 articles published since 1937. This ethnopharmacological study of the plant may create new insight into drug discovery to develop important novel leads against various biological targets.

scholarly journals Cytochrome P450 Hydroxylase TnmL Catalyzing Sequential Hydroxylation with an Additional Proofreading Activity in Tiancimycin Biosynthesis

2021 ◽  
Author(s):  
Thibault Annaval ◽  
Christiana N. Teijaro ◽  
Ajeeth Adhikari ◽  
Xiaohui Yan ◽  
Jian-Jun Chen ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Cytochrome P450 Hydroxylase

A review on phyto-pharmacology of Oxalis corniculata

2021 ◽  
Vol 24 ◽  
Author(s):  
Iqra Sarfraz ◽  
Azhar Rasul ◽  
Ghulam Hussain ◽  
Muhammad Ajmal Shah ◽  
Bushra Nageen ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Biological Activities ◽  
Chemical Constituents ◽  
Current Effort ◽  
Isolation And Characterization ◽  
Anti Cancer ◽  
Anti Oxidant ◽  
Anti Fungal ◽  
Warm Temperate

: Oxalis corniculata (Oxalidaceae) is a small decumbent and delicate appearing medicinal herb flourishing in warm temperate and tropical domains such as Pakistan and India. Main bioactive chemical constituents of Oxalis plant include several alkaloids, flavonoids, terpenoids, cardiac glycosides, saponins, phlobatannins along with steroids. Due to its polyphenolic, glycosides and flavonoid profile, it is proved to be protective in numerous ailments and exhibit various biological activities such as anti-fungal, anti-cancer, anti-oxidant, anti-bacterial, anti-diabetic, and cardioprotective. Moreover, bioactive phytochemicals from this plant possess significant wound healing potential. Our current effort intends to emphasize on the immense significance of this plant species, which have not been the subject matter of clinical trials and effective pharmacological studies, even though its favored usage has been stated. This review proposes that Oxalis corniculata possess potential for the cure of various diseases, however, further researches on isolation and characterization of bioactive compounds along with pre-clinical trials are compulsory to figure out its pharmacological applications.

scholarly journals Role of Cytochrome P450 Hydroxylase in the Decreased Accumulation of Vitamin E in Muscle from Turkeys Compared to that from Chickens

2016 ◽  
Vol 64 (3) ◽  
pp. 671-680 ◽  
Author(s):  
Dale M. Perez ◽  
Mark P. Richards ◽  
Robert S. Parker ◽  
Mark E. Berres ◽  
Aaron T. Wright ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Vitamin E ◽  
Cytochrome P450 Hydroxylase

DNA Interaction and Biological Activities of Heteroleptic Palladium (II) Complexes

2021 ◽  
Vol 43 (2) ◽  
pp. 227-227
Author(s):  
Muhammad Anwar Saeed Muhammad Anwar Saeed ◽  
Hizbullah Khan Hizbullah Khan ◽  
Muhammad Sirajuddin Muhammad Sirajuddin ◽  
Syed Muhammad Salman Syed Muhammad Salman
Keyword(s):  
Biological Activities ◽  
Thiobarbituric Acid ◽  
Dna Interaction ◽  
Log P ◽  
Bacterial Strains ◽  
Experimental Conditions ◽  
H2o2 Treatment ◽  
Biological Studies ◽  
Almost All ◽  
Anti Fungal

The manuscript describes the binding of DNA as well as biological studies of some mixed ligand dithiocarbamate Palladium (II) complexes (1-5). The observed compounds are of general formulae [PdCl(DT)(PR3)]. The dithiocarbamate “DT” and “PR3” groups are varied among the studied complexes as DT = bis[(2-methoxyethyl) dithiocarbamate)] (1 and 2), dibutyl dithiocarbamate (4 and 5), bis[(2-ethyl) hexyl dithiocarbamate)] (3); PR3 = triphenyl phosphine (1), benzy diphenyl phosphine (2), diphenyl-tert-butyl phpsphine (3), diphenyl-p-tolyl phosphine (4) and diphenyl-2-methoxy phenyl phosphine (5). The synthesized complexes were screened for DNA binding study via (UV Visible spectrophotometry and Viscometery) and biological activities such as anti-bacterial and anti-fungal, Molinspiration calculations and antioxidant potencies stimulated by hydrogen peroxide in human blood lymphocytes. In case of drug DNA interaction, complexes showed some sort of interaction with DNA solution. Almost all the complexes exhibited moderate antifungal and antibacterial behavior (against Gram positive and negative bacterial strains). The Molinspiration calculation study revealed that the said Pd (II) mixed complexes are biologically significant drugs having adequate molecular properties regarding drug likeness, except the log P values of complexes 3-5 because some structural adjustments must be done for enhancement of their bioavailability and hydrophilic nature. Regarding the antioxidant potential of complexes 1, 2 and 4, the H2O2 treatment of complexes violently decreased the action of antioxidant enzymes, superoxide dismutase and catalase and enhanced the level of thiobarbituric acid-reacting substances. Under experimental conditions, we conclude that all complexes act as anti-mutagens as they significantly suppress H2O2-induced oxidative damage at non-genotoxic concentrations.

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