scholarly journals Yeasts with Fermentative Potential Associated with Fruits of Camu-Camu (Myrciaria Dubia, Kunth) from North of Brazilian Amazon

2021 ◽  
Author(s):  
Italo Thiago Silveira Rocha Matos ◽  
Vanderly Andrade de Souza ◽  
Giovana do Rosário D'Ângelo ◽  
Spartaco Astolfi-Filho ◽  
Marcos José Salgado Vital
Keyword(s):  
Ethanol Yield ◽  
Brazilian Amazon ◽  
Alcoholic Beverages ◽  
Pichia Kudriavzevii ◽  
Biotechnological Potential ◽  
Myrciaria Dubia ◽  
Sequences Analysis ◽  
Candida Orthopsilosis ◽  
High Ethanol ◽  
Rare Report

Abstract PurposeConsidering the high biotechnological potential of yeasts associated to edible fruits, a screening for these microbes able to alcoholic fermentation was performed in ripe fruits of camu-camu.MethodsFruits of camu-camu (Myrciaria dubia, Kunth) were collected in north of brazilian Amazon, in floodplain of Cauamé river. Yeasts were isolated, and fermentation capability was evaluated using Durham tubes. Quantitative assays were performed to calculate ethanol yield (g.g–1), specific growth rate (h–1) and ethanol productivity (g.L–1.h–1). Taxonomic identification was performed by ribosomal genes nucleotides sequences analysis by alignment using BLASTn.ResultsA total of fifteen yeasts colonies were isolated, and eight of them presented the ability to ferment glucose to ethanol. Six of them were identified as three different species: Candida orthopsilosis, Pichia kudriavzevii and Meyerozyma caribbica. When cultured in broth containing 180 g.L–1 of glucose, M. caribbica reaches 91.7 percent of the maximum theoretical ethanol concentration (84.4 g.L–1), presenting ethanol yield and productivity of 0.4688 g.g–1 and 0.781 g.L–1.h–1, respectively.ConclusionsThe endophytic microbiota of camu-camu includes C. orthopsilosis, P. kudriavzevii and M. caribbica. This paper is a rare report of C. orthopsilosis with endophytic habit, because most of the references indicate it as human pathogenic. Besides this, M. caribbica is a promising fermenter for alcoholic beverages, due to its osmotolerance and high ethanol yield. This is the first paper reporting endophytic yeasts associated with fruits of Myrciaria dubia.

scholarly journals Yeasts with Fermentative Potential Associated with Fruits of Camu-Camu (Myrciaria dubia, Kunth) from North of Brazilian Amazon

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Ítalo Thiago Silveira Rocha Matos ◽  
Vanderly Andrade de Souza ◽  
Giovana do Rosário D’Angelo ◽  
Spartaco Astolfi Filho ◽  
Edson Júnior do Carmo ◽  
...  
Keyword(s):  
Ethanol Yield ◽  
Brazilian Amazon ◽  
Ribosomal Gene ◽  
Alcoholic Beverages ◽  
Nucleotide Sequence Analysis ◽  
Pichia Kudriavzevii ◽  
Biotechnological Potential ◽  
Myrciaria Dubia ◽  
Candida Orthopsilosis ◽  
Rare Report

Considering the high biotechnological potential of yeasts associated to edible fruits, a screening for these microorganisms, capable of alcoholic fermentation, was performed in ripe fruits of camu-camu (Myrciaria dubia, Kunth). The fruits were collected from north of Brazilian Amazon, in the floodplain of the Cauamé River. Yeasts were isolated, and fermentation capability was evaluated using Durham tubes. Quantitative assays were performed to calculate ethanol yield (g g−1), specific growth rate (h−1), and ethanol productivity (g L−1·h−1). Taxonomic identification was performed by ribosomal gene nucleotide sequence analysis by alignment using BLASTN. A total of fifteen yeast colonies were isolated, and three of them presented promising ability to ferment glucose to ethanol. These isolates were identified as Candida orthopsilosis, Pichia kudriavzevii, and Meyerozyma caribbica. When cultured in broth containing 180 g·L−1 of glucose, M. caribbica CC003 reached 91.7 percent of the maximum theoretical ethanol concentration (84.4 g·L−1), presenting an ethanol yield and productivity of 0.4688 g·g−1 and 0.781 g·L−1·h−1, respectively. These results indicate a promising potential of this isolate for bioprocess applications. This paper is a rare report of C. orthopsilosis with endophytic habit because most of the references indicate it as a human pathogen. Besides this, M. caribbica is a promising fermenter for alcoholic beverages due to its osmotolerance and high ethanol yield. This is the first paper reporting endophytic yeasts associated with fruits of Myrciaria dubia.

scholarly journals Combining Ability Studies for Identifying High Sugar Yielding Sweet Sorghum [Sorghum bicolor (L.) Moench] Genotypes

2020 ◽  
pp. 43-49
Author(s):  
B. C. Nandeshwar ◽  
Beka Biri ◽  
Alemayehu Dugassa
Keyword(s):  
Combining Ability ◽  
Sweet Sorghum ◽  
Gene Action ◽  
Ethanol Yield ◽  
Breeding Programme ◽  
Combining Ability Analysis ◽  
Half Diallel ◽  
High Ethanol ◽  
Sorghum Bicolor L ◽  
Pedigree Breeding

Combining ability analysis provides information about the gene action involved in the expression of a trait and facilitates breeding of superior cultivars. Hence, 45 hybrids evolved from 10 parent half-diallel were evaluated for combining ability to identify good general combiners and superior cross combinations for high ethanol yield from sweet sorghum. RSSV-21-2 has been identified as the best general combiner. It can be used in pedigree breeding programme for the incorporation of desired traits for enhancing ethanol yield. ARS-SS-35-1 × NSS-218 and ARS-SS-83 × NSS-221-2 have been identified as the best specific combinations. These could be exploited in heterosis breeding programme.

Experimental and Kinetic Production of Ethanol Using Mucilage Juice Residues from Cocoa Processing

2018 ◽  
Vol 16 (11) ◽  
Author(s):  
Teresa Romero Cortes ◽  
Jaime A. Cuervo-Parra ◽  
Víctor José Robles-Olvera ◽  
Eduardo Rangel Cortes ◽  
Pablo A. López Pérez
Keyword(s):  
Ethanol Yield ◽  
Scale Up ◽  
Pilot Scale ◽  
Product Formation ◽  
Model Parameters ◽  
Pichia Kudriavzevii ◽  
Proposed Model ◽  
Analysis Of Results ◽  
Residual Plots ◽  
Kinetics Of

AbstractEthanol was produced using mucilage juice residues from processed cocoa with Pichia kudriavzevii in batch fermentation. Experimental results showed that maximum ethanol concentration was 13.8 g/L, ethanol yield was 0.50 g-ethanol/g glucose with a productivity of 0.25 g/L h. Likewise, a novel phenomenological model based on the mechanism of multiple parallel coupled reactions was used to describe the kinetics of substrate, enzyme, biomass and product formation. Model parameters were optimized by applying the Levenberg-Marquardt approach. Analysis of results was based on statistical metrics (such as confidence interval), sensitivity and by comparing calculated curves with the experimental data (residual plots). The efficacy of the proposed mathematical model was statistically evaluated using the dimensionless coefficient for efficiency. Results indicated that the proposed model can be applied as a way of augmenting bioethanol production from laboratory scale up to semi-pilot scale.

scholarly journals Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Liang Tian ◽  
Beth Papanek ◽  
Daniel G. Olson ◽  
Thomas Rydzak ◽  
Evert K. Holwerda ◽  
...  
Keyword(s):  
Clostridium Thermocellum ◽  
Ethanol Yield ◽  
High Ethanol

scholarly journals Isolation and Some Properties of Mutants for High Ethanol Yield from the Cryophilic Wine Yeast Saccharomyces bayanus

1995 ◽  
Vol 90 (5) ◽  
pp. 381-386
Author(s):  
Akinari OSHIDA ◽  
Munekazu KISHIMOTO ◽  
Fujitoshi YANAGIDA ◽  
Takashi SHINOHARA ◽  
Shoji GOTO
Keyword(s):  
Ethanol Yield ◽  
Wine Yeast ◽  
Saccharomyces Bayanus ◽  
High Ethanol

scholarly journals Obtaining monokaryotic and dikaryotic mycelial cultures of two Amazonian strains of Geastrum (Geastraceae, Basidiomycota)

2020 ◽  
Vol 50 (1) ◽  
pp. 61-67
Author(s):  
Marcos Diones Ferreira SANTANA ◽  
Ruby VARGAS-ISLA ◽  
Janaina da Costa NOGUEIRA ◽  
Thiago ACCIOLY ◽  
Bianca Denise Barbosa da SILVA ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Brazilian Amazon ◽  
Potato Dextrose Agar ◽  
Morphological Description ◽  
Agar Culture ◽  
Biotechnological Potential ◽  
Sexual Crosses ◽  
High Diversity

ABSTRACT The high diversity of the genus Geastrum and the difficulty of obtaining mycelial cultures impairs the study of the ecophysiology and the exploration of the biotechnological potential of the taxon. In this study, different culture media were tested to obtain mycelial cultures for G. lloydianum and G. subiculosum collected in the Brazilian Amazon. Data on spore germination, and isolation of monokaryotic cultures and in vitro sexual reproduction are presented, as well as a brief morphological description of the cultures obtained. For both species, Potato Dextrose Agar (PDA) was the most promising of the tested culture media. The highest growth in agar culture ever recorded for this genus is reported (4.9 mm per week for G. lloydianum and 7.5 mm for G. subiculosum). In the PDA culture medium, spores germinated after 35-40 days of incubation and the isolation of monokaryotic cultures of the two species, as well as in vitro sexual crosses, were successfully performed.

scholarly journals Non-alcoholic components of Pelinkovac, a Croatian wormwood-based strong liquor, counteract the inhibitory effect of high ethanol concentration on catalase in vitro

2022 ◽  
Author(s):  
Jan Homolak ◽  
Ana Babic Perhoc ◽  
Mihovil Joja ◽  
Ivan Kodvanj ◽  
Karlo Toljan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Ethanol Concentration ◽  
Inhibitory Effect ◽  
Alcoholic Beverages ◽  
Acidic Ph ◽  
Inhibitory Effects ◽  
High Ethanol ◽  
Antioxidant Enzyme Catalase

Antioxidant enzyme catalase protects the cells against alcohol-induced oxidative stress by scavenging free radicals and metabolizing alcohol. Concentrations of ethanol present in alcoholic beverages can inhibit catalase and foster oxidative stress and alcohol-induced injury. Non-alcoholic components of pelinkovac counteract the inhibitory effects of high ethanol concentration and acidic pH on catalase in vitro.

Genomics Perspectives of Bioethanol Producing Zymomonas Mobilis

2012 ◽  
pp. 209-233
Author(s):  
S. Sheik Asraf ◽  
K.N. Rajnish ◽  
P. Gunasekaran
Keyword(s):  
Functional Properties ◽  
Zymomonas Mobilis ◽  
Fossil Fuels ◽  
Ethanol Yield ◽  
Genomic Data ◽  
Continuous Increase ◽  
Renewable Source ◽  
Gram Negative ◽  
High Interest ◽  
High Ethanol

In recent years, there has been continuous increase in demand for fossil fuels that has led to the need for new potential fuel sources. Biofuels, in particular ethanol, are of high interest because of dwindling fossil fuels. Among the ethanol producers, Zymomonas mobilis has acquired greater interest because it is a renewable source of bioethanol. Zymomonas mobilis is an aerotolerant, gram-negative, ethanol producing bacterium that shows high ethanol yield, tolerance, and greater productivity. This chapter focuses on recent efforts made to engineer Z. mobilis, transcriptomic, genome-based metabolomic studies, and bioinformatics exploitation of the available genomic data for the production of bioethanol. Recently, several bioinformatics tools have been used to predict the functional properties of the carbohydrate active ethanologenic enzymes in Z. mobilis. A number of processes were used to study the functional properties of the ethanologenic enzymes of Z. mobilis. Thus, functional genomics seeks to apply technologies that would help to improve the production of bioethanol by Z. mobilis.

Genomics Perspectives of Bioethanol Producing Zymomonas Mobilis

2013 ◽  
pp. 1354-1377
Author(s):  
S. Sheik Asraf ◽  
K.N. Rajnish ◽  
P. Gunasekaran
Keyword(s):  
Functional Properties ◽  
Zymomonas Mobilis ◽  
Fossil Fuels ◽  
Ethanol Yield ◽  
Genomic Data ◽  
Continuous Increase ◽  
Renewable Source ◽  
Gram Negative ◽  
High Interest ◽  
High Ethanol

In recent years, there has been continuous increase in demand for fossil fuels that has led to the need for new potential fuel sources. Biofuels, in particular ethanol, are of high interest because of dwindling fossil fuels. Among the ethanol producers, Zymomonas mobilis has acquired greater interest because it is a renewable source of bioethanol. Zymomonas mobilis is an aerotolerant, gram-negative, ethanol producing bacterium that shows high ethanol yield, tolerance, and greater productivity. This chapter focuses on recent efforts made to engineer Z. mobilis, transcriptomic, genome-based metabolomic studies, and bioinformatics exploitation of the available genomic data for the production of bioethanol. Recently, several bioinformatics tools have been used to predict the functional properties of the carbohydrate active ethanologenic enzymes in Z. mobilis. A number of processes were used to study the functional properties of the ethanologenic enzymes of Z. mobilis. Thus, functional genomics seeks to apply technologies that would help to improve the production of bioethanol by Z. mobilis.

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