scholarly journals Isolation and Some Properties of Mutants for High Ethanol Yield from the Cryophilic Wine Yeast Saccharomyces bayanus

1995 ◽  
Vol 90 (5) ◽  
pp. 381-386
Author(s):  
Akinari OSHIDA ◽  
Munekazu KISHIMOTO ◽  
Fujitoshi YANAGIDA ◽  
Takashi SHINOHARA ◽  
Shoji GOTO
Keyword(s):  
Ethanol Yield ◽  
Wine Yeast ◽  
Saccharomyces Bayanus ◽  
High Ethanol

Viral dsRNA in the wine yeast Saccharomyces bayanus var. uvarum

2007 ◽  
Vol 158 (8-9) ◽  
pp. 638-643 ◽  
Author(s):  
Yuliya V. Ivannikova ◽  
Elena S. Naumova ◽  
Gennadi I. Naumov
Keyword(s):  
Wine Yeast ◽  
Viral Dsrna ◽  
Saccharomyces Bayanus

scholarly journals Combining Ability Studies for Identifying High Sugar Yielding Sweet Sorghum [Sorghum bicolor (L.) Moench] Genotypes

2020 ◽  
pp. 43-49
Author(s):  
B. C. Nandeshwar ◽  
Beka Biri ◽  
Alemayehu Dugassa
Keyword(s):  
Combining Ability ◽  
Sweet Sorghum ◽  
Gene Action ◽  
Ethanol Yield ◽  
Breeding Programme ◽  
Combining Ability Analysis ◽  
Half Diallel ◽  
High Ethanol ◽  
Sorghum Bicolor L ◽  
Pedigree Breeding

Combining ability analysis provides information about the gene action involved in the expression of a trait and facilitates breeding of superior cultivars. Hence, 45 hybrids evolved from 10 parent half-diallel were evaluated for combining ability to identify good general combiners and superior cross combinations for high ethanol yield from sweet sorghum. RSSV-21-2 has been identified as the best general combiner. It can be used in pedigree breeding programme for the incorporation of desired traits for enhancing ethanol yield. ARS-SS-35-1 × NSS-218 and ARS-SS-83 × NSS-221-2 have been identified as the best specific combinations. These could be exploited in heterosis breeding programme.

scholarly journals Simultaneous achievement of high ethanol yield and titer in Clostridium thermocellum

2016 ◽  
Vol 9 (1) ◽  
Author(s):  
Liang Tian ◽  
Beth Papanek ◽  
Daniel G. Olson ◽  
Thomas Rydzak ◽  
Evert K. Holwerda ◽  
...  
Keyword(s):  
Clostridium Thermocellum ◽  
Ethanol Yield ◽  
High Ethanol

scholarly journals Yeasts with Fermentative Potential Associated with Fruits of Camu-Camu (Myrciaria Dubia, Kunth) from North of Brazilian Amazon

2021 ◽  
Author(s):  
Italo Thiago Silveira Rocha Matos ◽  
Vanderly Andrade de Souza ◽  
Giovana do Rosário D'Ângelo ◽  
Spartaco Astolfi-Filho ◽  
Marcos José Salgado Vital
Keyword(s):  
Ethanol Yield ◽  
Brazilian Amazon ◽  
Alcoholic Beverages ◽  
Pichia Kudriavzevii ◽  
Biotechnological Potential ◽  
Myrciaria Dubia ◽  
Sequences Analysis ◽  
Candida Orthopsilosis ◽  
High Ethanol ◽  
Rare Report

Abstract PurposeConsidering the high biotechnological potential of yeasts associated to edible fruits, a screening for these microbes able to alcoholic fermentation was performed in ripe fruits of camu-camu.MethodsFruits of camu-camu (Myrciaria dubia, Kunth) were collected in north of brazilian Amazon, in floodplain of Cauamé river. Yeasts were isolated, and fermentation capability was evaluated using Durham tubes. Quantitative assays were performed to calculate ethanol yield (g.g–1), specific growth rate (h–1) and ethanol productivity (g.L–1.h–1). Taxonomic identification was performed by ribosomal genes nucleotides sequences analysis by alignment using BLASTn.ResultsA total of fifteen yeasts colonies were isolated, and eight of them presented the ability to ferment glucose to ethanol. Six of them were identified as three different species: Candida orthopsilosis, Pichia kudriavzevii and Meyerozyma caribbica. When cultured in broth containing 180 g.L–1 of glucose, M. caribbica reaches 91.7 percent of the maximum theoretical ethanol concentration (84.4 g.L–1), presenting ethanol yield and productivity of 0.4688 g.g–1 and 0.781 g.L–1.h–1, respectively.ConclusionsThe endophytic microbiota of camu-camu includes C. orthopsilosis, P. kudriavzevii and M. caribbica. This paper is a rare report of C. orthopsilosis with endophytic habit, because most of the references indicate it as human pathogenic. Besides this, M. caribbica is a promising fermenter for alcoholic beverages, due to its osmotolerance and high ethanol yield. This is the first paper reporting endophytic yeasts associated with fruits of Myrciaria dubia.

Genomics Perspectives of Bioethanol Producing Zymomonas Mobilis

2012 ◽  
pp. 209-233
Author(s):  
S. Sheik Asraf ◽  
K.N. Rajnish ◽  
P. Gunasekaran
Keyword(s):  
Functional Properties ◽  
Zymomonas Mobilis ◽  
Fossil Fuels ◽  
Ethanol Yield ◽  
Genomic Data ◽  
Continuous Increase ◽  
Renewable Source ◽  
Gram Negative ◽  
High Interest ◽  
High Ethanol

In recent years, there has been continuous increase in demand for fossil fuels that has led to the need for new potential fuel sources. Biofuels, in particular ethanol, are of high interest because of dwindling fossil fuels. Among the ethanol producers, Zymomonas mobilis has acquired greater interest because it is a renewable source of bioethanol. Zymomonas mobilis is an aerotolerant, gram-negative, ethanol producing bacterium that shows high ethanol yield, tolerance, and greater productivity. This chapter focuses on recent efforts made to engineer Z. mobilis, transcriptomic, genome-based metabolomic studies, and bioinformatics exploitation of the available genomic data for the production of bioethanol. Recently, several bioinformatics tools have been used to predict the functional properties of the carbohydrate active ethanologenic enzymes in Z. mobilis. A number of processes were used to study the functional properties of the ethanologenic enzymes of Z. mobilis. Thus, functional genomics seeks to apply technologies that would help to improve the production of bioethanol by Z. mobilis.

Genomics Perspectives of Bioethanol Producing Zymomonas Mobilis

2013 ◽  
pp. 1354-1377
Author(s):  
S. Sheik Asraf ◽  
K.N. Rajnish ◽  
P. Gunasekaran
Keyword(s):  
Functional Properties ◽  
Zymomonas Mobilis ◽  
Fossil Fuels ◽  
Ethanol Yield ◽  
Genomic Data ◽  
Continuous Increase ◽  
Renewable Source ◽  
Gram Negative ◽  
High Interest ◽  
High Ethanol

In recent years, there has been continuous increase in demand for fossil fuels that has led to the need for new potential fuel sources. Biofuels, in particular ethanol, are of high interest because of dwindling fossil fuels. Among the ethanol producers, Zymomonas mobilis has acquired greater interest because it is a renewable source of bioethanol. Zymomonas mobilis is an aerotolerant, gram-negative, ethanol producing bacterium that shows high ethanol yield, tolerance, and greater productivity. This chapter focuses on recent efforts made to engineer Z. mobilis, transcriptomic, genome-based metabolomic studies, and bioinformatics exploitation of the available genomic data for the production of bioethanol. Recently, several bioinformatics tools have been used to predict the functional properties of the carbohydrate active ethanologenic enzymes in Z. mobilis. A number of processes were used to study the functional properties of the ethanologenic enzymes of Z. mobilis. Thus, functional genomics seeks to apply technologies that would help to improve the production of bioethanol by Z. mobilis.

scholarly journals Both adhE and a Separate NADPH-Dependent Alcohol Dehydrogenase Gene, adhA, Are Necessary for High Ethanol Production in Thermoanaerobacterium saccharolyticum

2016 ◽  
Vol 199 (3) ◽  
Author(s):  
Tianyong Zheng ◽  
Daniel G. Olson ◽  
Sean J. Murphy ◽  
Xiongjun Shao ◽  
Liang Tian ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Ethanol Production ◽  
Ethanol Yield ◽  
Single Gene ◽  
Maximum Yield ◽  
Primary Alcohol ◽  
Content Type ◽  
Thermoanaerobacterium Saccharolyticum ◽  
Dehydrogenase Gene ◽  
High Ethanol

ABSTRACT Thermoanaerobacterium saccharolyticum has been engineered to produce ethanol at about 90% of the theoretical maximum yield (2 ethanol molecules per glucose equivalent) and a titer of 70 g/liter. Its ethanol-producing ability has drawn attention to its metabolic pathways, which could potentially be transferred to other organisms of interest. Here, we report that the iron-containing AdhA is important for ethanol production in the high-ethanol strain of T. saccharolyticum (LL1049). A single-gene deletion of adhA in LL1049 reduced ethanol production by ∼50%, whereas multiple gene deletions of all annotated alcohol dehydrogenase genes except adhA and adhE did not affect ethanol production. Deletion of adhA in wild-type T. saccharolyticum reduced NADPH-linked alcohol dehydrogenase (ADH) activity (acetaldehyde-reducing direction) by 93%. IMPORTANCE In this study, we set out to identify the alcohol dehydrogenases necessary for high ethanol production in T. saccharolyticum. Based on previous work, we had assumed that adhE was the primary alcohol dehydrogenase gene. Here, we show that both adhA and adhE are needed for high ethanol yield in the engineered strain LL1049. This is the first report showing adhA is important for ethanol production in a native adhA host, which has important implications for achieving higher ethanol yields in other microorganisms.

scholarly journals Bioethanol Production via Syngas Fermentation

2018 ◽  
Vol 156 ◽  
pp. 03025 ◽  
Author(s):  
Irika Anggraini ◽  
Made Tri Ari Penia Kresnowati ◽  
Ronny Purwadi ◽  
Tjandra Setiadi
Keyword(s):  
Ethanol Production ◽  
High Performance ◽  
Organic Nitrogen ◽  
Main Product ◽  
Ethanol Yield ◽  
Optimum Process ◽  
Clostridium Ljungdahlii ◽  
Buffer Composition ◽  
Organic Nitrogen Source ◽  
High Ethanol

Bioconversion of C-1 carbon in syngas through microbial fermentation presents a huge potential to be further explored for ethanol production. Syngas can be obtained from the gasification of lignocellulosic biomass, by which most of carbon content of the biomass was converted into CO and CO2. These gases could be further utilized by carbon-fixing microorganism such as Clostridium sp. to produce ethanol as the end product. In order to obtain an optimum process, a robust and high performance strain is required and thus high ethanol yield as the main product can be expected. In this study, series of batch fermentation was carried out to select high performance strains for ethanol production. Bottle serum fermentations were performed using CO-gas as the sole carbon source to evaluate the potential of some Clostridia species such as Clostridium ljungdahlii, C. ragsdalei, and C. carboxidovorans in producing ethanol at various concentration of yeast extract as the organic nitrogen source, salt concentration, and buffer composition. Strain with the highest ethanol production in the optimum media will be further utilized in the upscale fermentation.

scholarly journals Glycerol Overproduction by Engineered Saccharomyces cerevisiae Wine Yeast Strains Leads to Substantial Changes in By-Product Formation and to a Stimulation of Fermentation Rate in Stationary Phase

1999 ◽  
Vol 65 (1) ◽  
pp. 143-149 ◽  
Author(s):  
F. Remize ◽  
J. L. Roustan ◽  
J. M. Sablayrolles ◽  
P. Barre ◽  
S. Dequin
Keyword(s):  
Stationary Phase ◽  
Ethanol Yield ◽  
Biomass Concentration ◽  
Fold Increase ◽  
Wine Yeast ◽  
Product Formation ◽  
Gene Encoding ◽  
Haploid Strain ◽  
Fermentation Rate ◽  
Yeast Strains

ABSTRACT Six commercial wine yeast strains and three nonindustrial strains (two laboratory strains and one haploid strain derived from a wine yeast strain) were engineered to produce large amounts of glycerol with a lower ethanol yield. Overexpression of the GPD1 gene, encoding a glycerol-3-phosphate dehydrogenase, resulted in a 1.5- to 2.5-fold increase in glycerol production and a slight decrease in ethanol formation under conditions simulating wine fermentation. All the strains overexpressing GPD1 produced a larger amount of succinate and acetate, with marked differences in the level of these compounds between industrial and nonindustrial engineered strains. Acetoin and 2,3-butanediol formation was enhanced with significant variation between strains and in relation to the level of glycerol produced. Wine strains overproducing glycerol at moderate levels (12 to 18 g/liter) reduced acetoin almost completely to 2,3-butanediol. A lower biomass concentration was attained byGPD1-overexpressing strains, probably due to high acetaldehyde production during the growth phase. Despite the reduction in cell numbers, complete sugar exhaustion was achieved during fermentation in a sugar-rich medium. Surprisingly, the engineered wine yeast strains exhibited a significant increase in the fermentation rate in the stationary phase, which reduced the time of fermentation.

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