Achieving high ethanol yield by co-feeding corncob residues and tea-seed cake at high-solids simultaneous saccharification and fermentation

Ethanol Yield ◽

Matter Content ◽

Raw Material ◽

Process Efficiency ◽

Dry Matter Content ◽

Sem Images ◽

Abstract The rising population and increasing demand for food place added pressure on the agricultural sector to maintain high process efficiency while implementing environmentally friendly methods. In this study, we investigate the effect of pre-hydrolysis of native rye starch and its influence on the yield of ethanol obtained by simultaneous saccharification and fermentation (SSF) from high gravity rye mashes with 25% and 28% w w−1 dry matter content. Fermentation was carried out in a 3-day system at a temperature of 35 ± 1 °C using the dry distillery yeast Ethanol Red (Saccharomyces cerevisiae). The characteristics of the tested raw material and changes in the native rye starch during enzymatic hydrolysis were analyzed using a scanning electron microscope (SEM). The SEM images revealed characteristic changes on the surface of the starch, which was found to have a layered structure, as well as interesting behavior by the yeast during SSF when the glucose concentration in the environment was lowered. Both in the mashes with 25% and 28% w w−1 dry matter, starch pre-hydrolysis did not significantly increase either the initial amounts of sugars available to the yeast or the fermentation efficiency and ethanol yield in comparison to the mashes without this pre-treatment.

Stimulatory effects of rhamnolipid on corncob residues ethanol production via high-solids simultaneous saccharification and fermentation

Fuel ◽

10.1016/j.fuel.2019.116091 ◽

2019 ◽

Vol 257 ◽

pp. 116091 ◽

Cited By ~ 3

Author(s):

Tianran Zheng ◽

Fuhou Lei ◽

Pengfei Li ◽

Shijie Liu ◽

Jianxin Jiang

Keyword(s):

Ethanol Production ◽

High Solids ◽

Improved Ethanol Production Based on High Solids Fed-Batch Simultaneous Saccharification and Fermentation with Alkali-Pretreated Sugarcane Bagasse

BioResources ◽

10.15376/biores.11.1.2548-2556 ◽

2016 ◽

Vol 11 (1) ◽

Author(s):

Yunyun Liu ◽

Jingliang Xu Xu ◽

Yu Zhang ◽

Minchao He ◽

Cuiyi Liang ◽

...

Keyword(s):

Ethanol Production ◽

Sugarcane Bagasse ◽

High Solids ◽

Fed Batch ◽

Pretreated Sugarcane Bagasse ◽

Fuel Ethanol Production from Wet Oxidised Corn Stover by S. Cerevisiae

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.343-344.963 ◽

2011 ◽

Vol 343-344 ◽

pp. 963-967 ◽

Cited By ~ 1

Author(s):

Zhang Qiang ◽

Anne Belinda Thomsen

Keyword(s):

Ethanol Production ◽

Corn Stover ◽

Ethanol Concentration ◽

Ethanol Yield ◽

Liquid Fraction ◽

Raw Material ◽

Wet Oxidation ◽

Inhibition Effect ◽

In order to find out appropriate process for ethanol production from corn stover, wet oxidation（195°C，15 minutes）and simultaneous sacchariﬁcation and fermentation (SSF) was carried out to produce ethanol. The results showed that the cellulose recovery of 92.9% and the hemicellulose recovery of 74.6% were obtained after pretreatment. 86.5% of cellulose was remained in the solid cake . After 24h hydrolysis at 50°C using cellulase（Cellubrix L）,the achieved conversion of cellulose to glucose was 64.8%. Ethanol production was evaluated from dried solid cake and the hydrolysate was employed as liquid fraction . After 142 h of SSF with substrate concentration of 8% (W/V), ethanol yield of 73.1 % of the theoretical based on glucose in the raw material was obtained by S. cerevisiae(ordinary baker’ yeast) . The corresponding ethanol concentration and volumetric productivity were 17.2g/L and 0.121g/L.h respectively. The estimated total ethanol production was 257.7 kg/ton raw material by assuming consumption of both C-6 and C-5. No obvious inhibition effect occurred during SSF. These instructions give you the basic guidelines for preparing papers for WCICA/IEEE conference proceedings.

High solids simultaneous saccharification and fermentation of pretreated wheat straw to ethanol

Applied Biochemistry and Biotechnology ◽

10.1007/bf02950778 ◽

1992 ◽

Vol 33 (2) ◽

pp. 67-81 ◽

Cited By ~ 111

Author(s):

A. Mohagheghi ◽

M. Tucker ◽

K. Grohmann ◽

C. Wyman

Keyword(s):

Wheat Straw ◽

High Solids ◽

Pretreated Wheat Straw ◽

Production of ethanol from Jerusalem artichoke by mycelial pellets

Scientific Reports ◽

10.1038/s41598-019-55117-7 ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Chao Zhang ◽

Daoji Wu ◽

Hongqi Yang ◽

Huixue Ren

Keyword(s):

Lower Cost ◽

Volume Fraction ◽

Ethanol Yield ◽

New Technology ◽

Jerusalem Artichoke ◽

Mycelial Pellets ◽

Repeated Use ◽

Easy Operation ◽

AbstractMycelial pellets formed by Aspergillus niger A-15 were used to immobilize the ethanol producing yeast Saccharomyces cerevisiae C-15. The operation parameters, such as agitation speed, temperature and mixed proportion of strains were studied. The optimal adsorption 66.9% was obtained when speed was 80r/min, temperature was 40 °C and mixed proportion(mycelial pellets: yeasts) was 1:10. With Jerusalem artichoke flour as substrate, 12.8% (V/V) of ethanol was obtained after 48 h by simultaneous saccharification and fermentation using mycelial pellets. And mycelial pellets could tolerate 19% (volume fraction) ethanol. The above results proved that this new technology was feasible, and it had the advantages of higher ethanol yield, long service life, repeated use, easy operation and lower cost in producing ethanol.

Bioethanol Production from Alkali-Treated Cotton Stalks at High Solids Loading Applying Non-isothermal Simultaneous Saccharification and Fermentation

Waste and Biomass Valorization ◽

10.1007/s12649-016-9818-4 ◽

2017 ◽

Vol 8 (6) ◽

pp. 1919-1929 ◽

Cited By ~ 11

Author(s):

Despoina Chilari ◽

Konstantinos Dimos ◽

Georgia Georgoula ◽

Thomas Paschos ◽

Diomi Mamma ◽

...

Keyword(s):

Bioethanol Production ◽

High Solids ◽

Solids Loading ◽

High Solids Loading ◽

Cotton Stalks ◽

Production of Bioethanol from Selected Lignocellulosic Agrowastes

Journal of Advances in Biology & Biotechnology ◽

10.9734/jabb/2019/v21i230089 ◽

2019 ◽

pp. 1-17

Author(s):

Olotu Emmanuel Juwon ◽

Olukunle Folake Oluwatoyin

Keyword(s):

Ethanol Yield ◽

Titratable Acidity ◽

Reducing Sugar ◽

Corn Cobs ◽

Orange Peels ◽

Total Titratable Acidity ◽

Cassava Peels ◽

Bioethanol Yield ◽

This study evaluated the ability of cassava peels, banana peels, orange peels and corn cobs hydrolysates to produce bioethanol. Fibre fractions analysis was carried out using standard methods. The samples were pre-treated with acid and base, followed by simultaneous saccharification and fermentation (SSF) for bioethanol production. During fermentation, pH, total titratable acidity, reducing sugar, microbial load and bioethanol yield were determined. The reducing sugar yield for Aspergillus niger and Bacillus cereus were 30.28 g and 13.35 g for corn cobs. The pH was observed to decrease during fermentation period with orange peels having the lowest pH of 2.6 after 240 hours of fermentation using A. Niger and S. cerevisiae, when B. cereus and S. Cerevisiae were used the pH was observed to be 4.10. Total titratable acidity showed increase in all the substrates, with corn cobs having the highest when B. cereus and S. cerevisiae were used (1.62), followed by cassava peels when A. niger and S. cerevisiae were used (1.52). Highest ethanol yield following simultaneous saccharification and fermentation with A. niger and S. cerevisiae was obtained in corn cobs with 17.43 g/100 g, while orange peels gave the lowest with 8.02 g/100 g, the ethanol yield from each substrates as well as the combined substrates were significantly different at p≤ 0.05. The combined substrates (1:1:1:1) gave the highest ethanol yield of 12.44 g/100 g using A. niger and S. cerevisiae. This study therefore revealed that A. niger had the highest bioethanol yield using corn cobs as the carbon source, therefore it could be used for mass bioethanol production.