scholarly journals Molecular characterization of Cotton leaf curl Multan virus and DNA-satellites complex associated enation leaf curl and yellow vein mosaic disease of hollyhock

2021 ◽  
Author(s):  
K.V. Ashwathappa ◽  
V Venkataravanappa ◽  
M. Nandan ◽  
H.D. Vinaykumar ◽  
K. S. Shankarappa ◽  
...  
Keyword(s):  
Complex Disease ◽  
Pcr Amplification ◽  
Mosaic Disease ◽  
Ornamental Plant ◽  
Yellow Vein ◽  
Cotton Leaf ◽  
New Delhi ◽  
The Status ◽  
Full Length Genome ◽  
Leaf Curl

Abstract Hollyhock is one important decorative plant grown in garden beds in different region of the the world. The ornamental plant is susceptible to many diseases caused by diverse pathogens. Among these viral pathogens can cause enormous damage to the ornamental plant. The aim of the present study was identification of begomovirus and DNA sateelites is associated with the yellow vein mosaic and enation leaf curl disease complex of hollyhock. The hollyhock plants showing the typical begomovirus-like symptoms were collected from Pusa campus, New Delhi (India). To know the status of the begomovirus, the total DNA isolated from the infected hollyhock was subjected to PCR amplification using primers specific to the begomovirus. The partial (1.2 kb) genome sequencing of ten hollyhock samples indicates the associated of begomovirus (nucleotide identities is more 95% among themselves). Therefore three representative samples (H1, H2, H3) full-length genome (DNA-A, betasatellite and alphasatellite) was amplified through RCA method. The pairwise comparision of complete genome of the begomoviruses, betasatellites and alphasatellites using Sequence Demarcation Tool (SDT) showed highest nucleotide (nt) identity of 88.0 to 92.7% (DNA-A) with Cotton leaf curl Multan virus, 92.5–96.7% with Ludwigia leaf distortion betasatellite and 90.4 to 93. 2% % with Ageratum enation alphasatellite. Further recombinantion analysis showed that the begomoviruses and DNA satellites under study was recombinants of previously reported begomoviruses and DNA sattelites. This is the first report of Cotton leaf curl Multan virus and DNA satellites associated complex disease of hollyhock in India.

Recombinant Tomato leaf curl New Delhi virus is associated with yellow vein mosaic disease of okra in India

2018 ◽  
Vol 104 ◽  
pp. 108-118 ◽  
Author(s):  
V. Venkataravanappa ◽  
C.N. Lakshminarayana Reddy ◽  
S. Saha ◽  
M. Krishna Reddy
Keyword(s):  
Mosaic Disease ◽  
Tomato Leaf ◽  
Yellow Vein ◽  
New Delhi ◽  
Tomato Leaf Curl ◽  
Yellow Vein Mosaic Disease ◽  
Leaf Curl

scholarly journals In silico evaluation of molecular virus–virus interactions taking place between Cotton leaf curl Kokhran virus- Burewala strain and Tomato leaf curl New Delhi virus

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12018
Author(s):  
Nida Fatima Ali ◽  
Rehan Zafar Paracha ◽  
Muhammad Tahir
Keyword(s):  
Protein Structures ◽  
Protein Docking ◽  
Tomato Leaf ◽  
Replication Initiation ◽  
Electrostatic Energy ◽  
Cotton Leaf ◽  
New Delhi ◽  
Virus Interactions ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Background Cotton leaf curl disease (CLCuD) is a disease of cotton caused by begomoviruses, leading to a drastic loss in the annual yield of the crop. Pakistan has suffered two epidemics of this disease leading to the loss of billions in annual exports. The speculation that a third epidemic of CLCuD may result as consequence of the frequent occurrence of Tomato leaf curl New Delhi virus (ToLCNDV) and Cotton leaf curl Kokhran Virus-Burewala Strain (CLCuKoV-Bu) in CLCuD infected samples, demand that the interactions taking between the two viruses be properly evaluated. This study is designed to assess virus-virus interactions at the molecular level and determine the type of co-infection taking place. Methods Based on the amino acid sequences of the gene products of both CLCuKoV-Bu and ToLCNDV, protein structures were generated using different software, i.e., MODELLER, I-TASSER, QUARKS, LOMETS and RAPTORX. A consensus model for each protein was selected after model quality assessment using ERRAT, QMEANDisCo, PROCHECK Z-Score and Ramachandran plot analysis. The active and passive residues in the protein structures were identified using the CPORT server. Protein–Protein Docking was done using the HADDOCK webserver, and 169 Protein–Protein Interaction (PPIs) were performed between the proteins of the two viruses. The docked complexes were submitted to the PRODIGY server to identify the interacting residues between the complexes. The strongest interactions were determined based on the HADDOCK Score, Desolvation energy, Van der Waals Energy, Restraint Violation Energy, Electrostatic Energy, Buried Surface Area and Restraint Violation Energy, Binding Affinity and Dissociation constant (Kd). A total of 50 ns Molecular Dynamic simulations were performed on complexes that exhibited the strongest affinity in order to validate the stability of the complexes, and to remove any steric hindrances that may exist within the structures. Results Our results indicate significant interactions taking place between the proteins of the two viruses. Out of all the interactions, the strongest were observed between the Replication Initiation protein (Rep) of CLCuKoV-Bu with the Movement protein (MP), Nuclear Shuttle Protein (NSP) of ToLCNDV (DNA-B), while the weakest were seen between the Replication Enhancer protein (REn) of CLCuKoV-Bu with the REn protein of ToLCNDV. The residues identified to be taking a part in interaction belonged to domains having a pivotal role in the viral life cycle and pathogenicity. It maybe deduced that the two viruses exhibit antagonistic behavior towards each other, and the type of infection may be categorised as a type of Super Infection Exclusion (SIE) or homologous interference. However, further experimentation, in the form of transient expression analysis, is needed to confirm the nature of these interactions and increase our understanding of the direct interactions taking place between two viruses.

scholarly journals Tomato leaf curl Gujarat virus, a New Begomovirus Species Causing a Severe Leaf Curl Disease of Tomato in Varanasi, India

2003 ◽  
Vol 93 (12) ◽  
pp. 1485-1495 ◽  
Author(s):  
S. Chakraborty ◽  
P. K. Pandey ◽  
M. K. Banerjee ◽  
G. Kalloo ◽  
C. M. Fauquet
Keyword(s):  
Tomato Leaf ◽  
Open Reading Frames ◽  
New Delhi ◽  
Tomato Plants ◽  
The Family ◽  
Infected Tomato ◽  
Tomato Leaf Curl ◽  
Full Length Genome ◽  
Severe Leaf ◽  
Leaf Curl

The biological and molecular properties of Tomato leaf curl Gujarat virus from Varanasi, India (ToLCGV-[Var]) were characterized. ToLCGV-[Var] could be transmitted by grafting and through whitefly transmission in a persistent manner. The full-length genome of DNA-A and DNA-B of ToLCGV-[Var] was cloned in pUC18. Sequence analysis revealed that DNA-A (AY190290) is 2,757 bp and DNA-B (AY190291) is 2,688 bp in length. ToLCGV-[Var] could infect and cause symptoms in tomato, pepper, Nicotiana benthamiana, and N. tabacum when partial tandem dimeric constructs of DNA-A and DNA-B were co-inoculated by particle bombardment. DNA-A alone also is infectious, but symptoms were milder and took longer to develop. ToLCGV-Var virus can be transmitted through sap inoculation from infected tomato plants to the above-mentioned hosts causing the same symptoms. Open reading frames (ORFs) in both DNA-A and DNA-B are organized similarly to those in other begomoviruses. DNA-A and DNA-B share a common region of 155 bp with only 60% sequence identity. DNA-B of ToLCGV-[Var] shares overall 80% identity with DNA-B of Tomato leaf curl New Delhi virus-Severe (ToLCNDV-Svr) and 75% with ToLCNDV-[Lucknow] (ToLCNDV-[Luc]). Comparison of DNA-A sequence with different begomoviruses indicates that ToLCGV-[Var] shares 84% identity with Tomato leaf curl Karnataka virus (ToLCKV) and 66% with ToLCNDV-Svr. ToLCGV-[Var] shares a maximum of 98% identity with another isolate of the same region (ToLCGV-[Mir]; AF449999) and 97% identity with one isolate from Gujarat (ToLCGV-[Vad]; AF413671). All three viruses belong to the same species that is distinct from all the other geminivirus species described so far in the genus Begomovirus of the family Geminiviridae. The name Tomato leaf curl Gujarat virus is proposed because the first sequence was taken from an isolate of Gujarat, India.

scholarly journals Molecular Characterization of a Begomovirus and Betasatellite Causing Yellow Vein Net Disease of Ageratum houstonianum

Plant Disease ◽  
2015 ◽  
Vol 99 (5) ◽  
pp. 627-631 ◽  
Author(s):  
Ashish Srivastava ◽  
Susheel Kumar ◽  
Shri Krishna Raj
Keyword(s):  
Sequence Analysis ◽  
Sequence Homology ◽  
Phylogenetic Relationships ◽  
Disease Incidence ◽  
Open Reading Frames ◽  
Yellow Vein ◽  
Full Length Genome ◽  
First Time ◽  
Leaf Curl

Ageratum houstonianum was introduced in India as an annual ornamental plant and is grown in beds for blue head flowers. Yellow vein net disease was observed on A. houstonianum plants with about 9.0% disease incidence during a survey in February 2012 at gardens of NBRI, Lucknow, India. Association of a begomovirus and betasatellite with the disease was characterized based on sequence analyses of their cloned full length genome isolated from diseased A. houstonianum. Sequence analysis of the begomovirus showed presence of the six open reading frames in its genome, similar to the arrangement of Old World begomoviruses. The begomoviral genome shared 95 to 97% sequence identities with various strains of Ageratum enation virus (AEV); however, it showed distinct phylogenetic relationships with them, and hence was identified as a variant of AEV based on more than 94% sequence homology, the criteria defined by ICTV. The sequence analysis of associated betasatellite revealed highest 93% sequence identity and close phylogenetic relationships with Ageratum leaf curl betasatellite (ALCB) molecules; therefore, it was identified as an isolate of ALCB (based on 93% sequence homology). Agroinfiltration of partial dimers of the AEV variant and ALCB induced similar systemic yellow vein net and leaf curl symptoms on A. houstonianum when infiltrated in combination, fulfilling Koch’s postulates. Characterization of AEV and ALCB causing yellow vein net disease of A. houstonianum is being reported for the first time.

scholarly journals Association of a recombinant Cotton leaf curl Bangalore virus with yellow vein and leaf curl disease of okra in India

2013 ◽  
Vol 24 (2) ◽  
pp. 188-198 ◽  
Author(s):  
V. Venkataravanappa ◽  
C. N. Lakshminarayana Reddy ◽  
A. Devaraju ◽  
Salil Jalali ◽  
M. Krishna Reddy
Keyword(s):  
Yellow Vein ◽  
Cotton Leaf ◽  
Leaf Curl Disease ◽  
Leaf Curl

scholarly journals Molecular evidence for association ofCotton leaf curl Alabad viruswith yellow vein mosaic disease of okra in North India

2013 ◽  
Vol 46 (1) ◽  
pp. 127-127
Author(s):  
V. Venkataravanappa ◽  
C.N. Lakshminarayana Reddy ◽  
P. Swarnalathaa ◽  
Devaraju ◽  
Salil Jalali ◽  
...  
Keyword(s):  
Mosaic Disease ◽  
Yellow Vein ◽  
North India ◽  
Molecular Evidence ◽  
Yellow Vein Mosaic Disease ◽  
Leaf Curl

scholarly journals Association ofBhendi yellow vein mosaic virusand Cotton leaf curl Multan betasatellite withCapsicum annuumFrom Kashmir valley, India

2015 ◽  
Vol 32 ◽  
pp. 9 ◽  
Author(s):  
Rahul Mohan Singh ◽  
Shivangi Sharma ◽  
Aijaz A. Zaidi ◽  
Aflaq Hamid ◽  
Vipin Hallan
Keyword(s):  
Yellow Vein ◽  
Cotton Leaf ◽  
Kashmir Valley ◽  
Leaf Curl

scholarly journals First Report of Association of Tomato leaf curl virus-New Delhi with Yellow Mosaic Disease of Luffa cylindrica in India

Plant Disease ◽  
2003 ◽  
Vol 87 (9) ◽  
pp. 1148-1148 ◽  
Author(s):  
S. S. Sohrab ◽  
B. Mandal ◽  
R. P. Pant ◽  
A. Varma
Keyword(s):  
Mosaic Disease ◽  
Tomato Leaf ◽  
New Delhi ◽  
Luffa Cylindrica ◽  
Yellow Mosaic Disease ◽  
Tomato Leaf Curl Virus ◽  
Sponge Gourd ◽  
Tomato Leaf Curl ◽  
Leaf Curl Virus ◽  
Leaf Curl

Sponge gourd (Luffa cylindrica), an important cucurbitaceous vegetable in India, is affected by a disease (2) causing yellow spots on newly emerged leaves, mosaic, mild leaf curling and distortion, small leaves, and misshapen fruits. Nearly 100% of sponge gourd plants were symptomatic in Delhi. Geminivirus-like particles were observed with electron microscopy of uranyl acetate-stained leaf-dip preparations of the diseased plants collected from experimental fields at the Indian Agricultural Research Institute in New Delhi during May and June of 2002. The virus was transmitted by the whitefly (Bemisia tabaci) to sponge and ridge gourd (L. acutangula) after an acquisition and inoculation access period of 24 h each. Whitefly-inoculated plants produced typical yellow mosaic symptoms and contained geminate particles. Nucleic acid extracted from the field-infected and experimentally infected plants hybridized with 32P-labeled probe to DNA-A of Indian cassava mosaic virus, suggesting association of a begomovirus. The viral DNA, isolated by the alkali denaturation method (1) from the experimentally infected sponge gourd plants, was cloned in pBS SK+ at the EcoRI site. A clone with an insert of 2,658 bp was sequenced (GenBank Accession Nos. AJ557219, AJ555488, and AY309957) which shared 89.6 to 95.1% identity with the DNA-A of different strains of Tomato leaf curl virus-New Delhi (ToLCV-NDe). The highest sequence identity (95.1%) was with the severe strain of ToLCV-NDe (GenBank Accession No. U15015). The data suggest that the begomovirus associated with the yellow mosaic disease of L. cylindrica in India is a putative strain of ToLCV-NDe. Reference: (1) K. M. Srivastava et al. J. Virol. Methods 51:297, 1995. (2) A. Varma and B. K. Giri. Virus diseases. Pages 225–245 in: Cucurbits. N. M. Nayar and T. A More, eds. Oxford and IBH Publishing House Private Ltd., New Delhi, India, 1998.

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