scholarly journals Electrophysiological, Behavioural and Biochemical Effect of Ocimum Basilicum Oil and Its Constituents Methyl Chavicol and Linalool on Musca Domestica L.

2021 ◽  
Author(s):  
Rajendran Senthoorraja ◽  
KESAVAN Subaharan ◽  
Sowmya Manjunath ◽  
Vppalayam Shanmugam Pragadheesh ◽  
Nandagopal Bakthavatsalam ◽  
...  
Keyword(s):  
Neuronal Response ◽  
Ocimum Basilicum ◽  
Contact Toxicity ◽  
Oviposition Deterrence ◽  
Detoxifying Enzymes ◽  
Biochemical Effect ◽  
Methyl Chavicol ◽  
Ovicidal Effect ◽  
Glutathione S Transferases

Abstract The Ocimum basilicum essential oil (EO) was evaluated for its biological effect on M. domestica. Characterization of O. basilicum EO revealed the presence of methyl chavicol (70.93%), linalool (9.34%), epi-α-cadinol (3.69 %), methyl eugenol (2.48%), γ-cadinene (1.67%), 1,8-cineole (1.30%) and (E)-β-ocimene (1.11%). The basil EO and its constituents methyl chavicol and linalool caused the neuronal response in female adults of M. domestica. Adult female flies showed reduced preference to food source laced with basil EO and methyl chavicol. Substrate treated with EO and methyl chavicol at 0.25% caused an oviposition deterrence of over 80%. The ovicidal effect was high in O. basilicum EO (EC50 9.74mg/dm3) followed by methyl chavicol (EC50 10.67mg/dm3) and linalool (EC50 13.57mg/dm3. On contact toxicity, adults exposed to EO (LD50 10.01 μg/adult) were more susceptible than to methyl chavicol and linalool (LD50 13.62 μg/adult and LD50 43.12 μg/adult respectively). EO and its constituents methyl chavicol and linalool induced the detoxifying enzymes Carboxyl esterase (Car E) and Glutathione S – transferases (GST)

scholarly journals Electrophysiological, behavioural and biochemical effect of Ocimum basilicum oil and its constituents methyl chavicol and linalool on Musca domestica L.

2021 ◽  
Author(s):  
Rajendran Senthoorraja ◽  
Kesavan Subaharan ◽  
Sowmya Manjunath ◽  
Vppalayam Shanmugam Pragadheesh ◽  
Nandagopal Bakthavatsalam ◽  
...  
Keyword(s):  
Musca Domestica ◽  
Ocimum Basilicum ◽  
Biochemical Effect ◽  
Methyl Chavicol

Essential oils from Ocimum basilicum cultivars: analysis of their composition and determination of the effect of the major compounds on Haemonchus contortus eggs

2021 ◽  
Vol 95 ◽  
Author(s):  
A.I.P. Sousa ◽  
C.R. Silva ◽  
H.N. Costa-Júnior ◽  
N.C.S. Silva ◽  
J.A.O. Pinto ◽  
...  
Keyword(s):  
Essential Oils ◽  
Haemonchus Contortus ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Ocimum Basilicum ◽  
Alternative Methods ◽  
Egg Hatch ◽  
Plant Essential Oils ◽  
Methyl Chavicol ◽  
The Individual

Abstract The continuous use of synthetic anthelmintics against gastrointestinal nematodes (GINs) has resulted in the increased resistance, which is why alternative methods are being sought, such as the use of natural products. Plant essential oils (EOs) have been considered as potential products for the control of GINs. However, the chemical composition and, consequently, the biological activity of EOs vary in different plant cultivars. The aim of this study was to evaluate the anthelmintic activity of EOs from cultivars of Ocimum basilicum L. and that of their major constituents against Haemonchus contortus. The EOs from 16 cultivars as well the pure compound linalool, methyl chavicol, citral and eugenol were used in the assessment of the inhibition of H. contortus egg hatch. In addition, the composition of three cultivars was simulated using a combination of the two major compounds from each. The EOs from different cultivars showed mean Inhibition Concentration (IC50) varying from 0.56 to 2.22 mg/mL. The cultivar with the highest egg-hatch inhibition, Napoletano, is constituted mainly of linalool and methyl chavicol. Among the individual compounds tested, citral was the most effective (IC50 0.30 mg/mL). The best combination of compounds was obtained with 11% eugenol plus 64% linalool (IC50 0.44 mg/mL), simulating the Italian Large Leaf (Richters) cultivar. We conclude that different cultivars of O. basilicum show different anthelmintic potential, with cultivars containing linalool and methyl chavicol being the most promising; and that citral or methyl chavicol isolated should also be considered for the development of new anthelmintic formulations.

Purification and characterization of glutathione S-transferases in human retina

1984 ◽  
Vol 3 (11) ◽  
pp. 1273-1280 ◽  
Author(s):  
Shivendra V. Singh ◽  
Dat D. Dao ◽  
Satish K. Srivastava ◽  
Yogesh C. Awasthi
Keyword(s):  
Human Retina ◽  
Purification And Characterization ◽  
Glutathione S Transferases

Characterization of new biodegradable edible film made from basil seed (Ocimum basilicum L.) gum

2014 ◽  
Vol 102 ◽  
pp. 199-206 ◽  
Author(s):  
Naimeh Khazaei ◽  
Mohsen Esmaiili ◽  
Zahra Emam Djomeh ◽  
Mehran Ghasemlou ◽  
Mohammad Jouki
Keyword(s):  
Ocimum Basilicum ◽  
Edible Film

scholarly journals Chemical and genetic stability of methyl chavicol-rich Indian basil (Ocimum basilicum var. CIM-Saumya) micropropagated in vitro

2017 ◽  
Vol 113 ◽  
pp. 186-191 ◽  
Author(s):  
M. Kumari ◽  
D. Agnihotri ◽  
C.S. Chanotiya ◽  
A.K. Mathur ◽  
R.K. Lal ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Ocimum Basilicum ◽  
Methyl Chavicol

Characterization of two μ class glutathione S-transferases from guinea pig lens

2000 ◽  
Vol 32 (1) ◽  
pp. 99-104 ◽  
Author(s):  
Naoto Noda ◽  
Hideki Adachi ◽  
Hirofumi Nanjo ◽  
Tomoyuki Terada
Keyword(s):  
Guinea Pig ◽  
Glutathione S Transferases

scholarly journals Karakterisasi Fraksi Aktif Antioksidan dari Ekstrak Etanol Biji Kemangi (Ocimum Basilicum L.)

2015 ◽  
pp. 39-49
Author(s):  
Dede Sukandar ◽  
Sandra Hermanto ◽  
Eka Rizki Amelia ◽  
Chitta Putri Novianti
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Ethanol Extract ◽  
Ocimum Basilicum ◽  
Total Phenolic ◽  
Solvent Fractionation ◽  
Butanol Extract ◽  
Dpph Method ◽  
Ic50 Values

Characterization of antioxidant compounds from the seeds of basil (Ocimum basilicum L.) has been done. Extraction is done by maceration method using ethanol solvent, fractionation by TLC and column chromatography, antioxidants test using DPPH method, and characterization of antioxidant compound using GCMS. Ethanol extract and results of fractionation ethanol extract of basil seeds using n-hexane, ethyl acetate, n-butanol and methanol-water extracts show that n-butanol extract has the highest antioxidant activity with IC50 values ​​of 41.90 ppm. Results of column chromatography n-butanol extract using n-hexane : ethyl acetate (1:9) as mobile phase yielded 5 fractions with fraction 4 (F4 isolate) has dominant stain of active antioxidants after being sprayed DPPH reagent, it had IC50 values ​​of 39,70 ppm and total phenolic content of 0,003 mg/g. Isolate F4 suspected contains two active compounds as antioxidant which is terpenoid and phenolic compound group, namely squalene and 1,4-di-tert-buthyl-phenol identified by GCMS.DOI :http://dx.doi.org/10.15408/jkv.v0i0.3598

Activities of Select Enzymes Involved in Insecticide Resistance in Spinosad-Resistant and -Susceptible Strains of Musca domestica (Diptera: Muscidae)

2019 ◽  
Author(s):  
Hafiz Azhar Ali Khan ◽  
Waseem Akram ◽  
Sajid Ali
Keyword(s):  
Insecticide Resistance ◽  
Musca Domestica ◽  
Enzyme Activities ◽  
Susceptible Strain ◽  
Detoxifying Enzymes ◽  
Mechanism Of Resistance ◽  
Mixed Function Oxidases ◽  
Glutathione S Transferases

Abstract A Musca domestica L. strain collected from Pakistan has recently been shown to be resistant to spinosad; however, there is scarce information about the mechanism of resistance. For this reason, we explored whether a metabolic-based mechanism was responsible by analyzing the activities of the metabolic detoxifying enzymes, carboxylesterases, glutathione S-transferases, and mixed-function oxidases, in both a spinosad-selected (Spin-SEL) strain of M. domestica and a susceptible counterpart (Lab-susceptible). The results revealed that both strains were statistically at par in terms of enzyme activities. The activity of carboxylesterases in the Lab-susceptible strain was 78.17 ± 3.06 in comparison to 79.16 ± 3.31 nmol min−1 mg−1 in the Spin-SEL strain. The activity of mixed-function oxidases was 51.58 ± 4.20 in the Lab-susceptible strain, whereas 54.33 ± 4.08 pmol min−1 mg−1 was recorded in the Spin-SEL strain. The activity of glutathione S-transferases was 86.50 ± 4.59 (Lab-susceptible) and 90.33 ± 2.81 nmol min−1 mg−1 (Spin-SEL). These results revealed that the studied enzymes might not be responsible for spinosad resistance in the studied strain of M. domestica. Therefore, studies should be extended to find out other possible mechanisms of spinosad resistance.

Characterization of glutathione S-transferases from day-old chick livers

Biochemistry ◽  
1990 ◽  
Vol 29 (3) ◽  
pp. 744-750 ◽  
Author(s):  
Li Hsueh Chang ◽  
Liu Fen Chuang ◽  
Chueh Ping Tsai ◽  
Chen Pei D. Tu ◽  
Ming F. Tam
Keyword(s):  
Glutathione S Transferases
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