scholarly journals Near Infra-Red Labelling and Tracking of Transplanted Corneal Endothelial Graft

2021 ◽  
Author(s):  
Maninder Bhogal ◽  
Heng-Pei Ang ◽  
Shu-Jun Lin ◽  
Lwin Chan ◽  
Khadijah Adnan ◽  
...  
Keyword(s):  
Real Time ◽  
Corneal Transplantation ◽  
Specular Microscopy ◽  
Post Transplantation ◽  
In Vivo Evaluation ◽  
Non Invasive ◽  
Real Time Visualization ◽  
Donor Graft

Abstract Following corneal transplantation, there is an initial decline in corneal endothelial cells (CECs) following graft preparation and surgery. Monitoring post-transplantation is only possible months after surgery by specular microscopy, which has a limited field of view. We have developed a labelling approach using 1,1’-dioctadecyl-3,3,3’,3’-tetramethylindotricarbocyanine iodide (DIR) dye solution, that enabled tracking of labelled CECs in vivo for at least one month. Initial in vitro optimization of dye concentration, cellular toxicity and real-time cell migration was assessed using propagated primary CECs. Subsequent in vivo evaluation of cellular labelling was assessed within a rabbit wound healing model. Finally, real-time visualization of human cadaver donor tissue incubated in DIR transplanted into rabbits was achieved using the Heidelberg Spectralis. Results revealed detectable fluorescence increased with concentration to a plateau of 100µg/ml, with no toxicity of CECs at any concentration evaluated. DIR-labelled CECs were detectable in vivo upto 1 month, and transplanted labelled donor graft could be visualized and were trackable in vivo. Acute endothelial rejection in 1 rabbit was evidenced by detectable DIR positive cells within the anterior chamber. DIR imaging allowed for detailed imaging of the transplanted corneal endothelium, and enabled non-invasive observation of the corneal endothelial morphology following transplantation.

scholarly journals Non-invasive, real-time reporting drug release in vitro and in vivo

2015 ◽  
Vol 51 (32) ◽  
pp. 6948-6951 ◽  
Author(s):  
Yanfeng Zhang ◽  
Qian Yin ◽  
Jonathan Yen ◽  
Joanne Li ◽  
Hanze Ying ◽  
...  
Keyword(s):  
Drug Release ◽  
Real Time ◽  
Near Infrared ◽  
Reporting System ◽  
Real Time Monitoring ◽  
Near Infrared Fluorescence ◽  
Non Invasive ◽  
Fluorescence Dye

Anin vitroandin vivodrug-reporting system is developed for real-time monitoring of drug release via the analysis of the concurrently released near-infrared fluorescence dye.

scholarly journals Validation of a Noninvasive, Real-Time Imaging Technology Using Bioluminescent Escherichia coli in the Neutropenic Mouse Thigh Model of Infection

2001 ◽  
Vol 45 (1) ◽  
pp. 129-137 ◽  
Author(s):  
H. L. Rocchetta ◽  
C. J. Boylan ◽  
J. W. Foley ◽  
P. W. Iversen ◽  
D. L. LeTourneau ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Real Time ◽  
Antimicrobial Agents ◽  
Viable Cell ◽  
Multicopy Plasmid ◽  
In Vivo Evaluation ◽  
Therapeutic Success ◽  
Cell Counts

ABSTRACT A noninvasive, real-time detection technology was validated for qualitative and quantitative antimicrobial treatment applications. Thelux gene cluster of Photorhabdus luminescenswas introduced into an Escherichia coli clinical isolate, EC14, on a multicopy plasmid. This bioluminescent reporter bacterium was used to study antimicrobial effects in vitro and in vivo, using the neutropenic-mouse thigh model of infection. Bioluminescence was monitored and measured in vitro and in vivo with an intensified charge-coupled device (ICCD) camera system, and these results were compared to viable-cell determinations made using conventional plate counting methods. Statistical analysis demonstrated that in the presence or absence of antimicrobial agents (ceftazidime, tetracycline, or ciprofloxacin), a strong correlation existed between bioluminescence levels and viable cell counts in vitro and in vivo. Evaluation of antimicrobial agents in vivo could be reliably performed with either method, as each was a sound indicator of therapeutic success. Dose-dependent responses could also be detected in the neutropenic-mouse thigh model by using either bioluminescence or viable-cell counts as a marker. In addition, the ICCD technology was examined for the benefits of repeatedly monitoring the same animal during treatment studies. The ability to repeatedly measure the same animals reduced variability within the treatment experiments and allowed equal or greater confidence in determining treatment efficacy. This technology could reduce the number of animals used during such studies and has applications for the evaluation of test compounds during drug discovery.

IN VITRO AND IN VIVO EVALUATION OF A NON INVASIVE RESPIRATORY MONITOR

1991 ◽  
Vol 75 (3) ◽  
pp. A398-A398
Author(s):  
M. Cigada ◽  
J. Sebastianelli ◽  
W. Fordyce ◽  
E. M. Camporesi
Keyword(s):  
In Vivo Evaluation ◽  
Non Invasive

scholarly journals Dynamic tracking of a magnetic micro-roller using ultrasound phase analysis

2021 ◽  
Author(s):  
Stefano Pane ◽  
Veronica Iacovacci ◽  
Mohammad Hasan Dad Ansari ◽  
Arianna Menciassi
Keyword(s):  
Real Time ◽  
Average Error ◽  
Contrast Resolution ◽  
Low Contrast ◽  
Non Invasive ◽  
Dynamic Tracking ◽  
Fine Control ◽  
Real Time Visualization ◽  
Acoustic Phase

Abstract Microrobots (MRs) have attracted significant interest for their potentialities in diagnosis and non-invasive intervention in hard-to-reach body areas. Fine control of biomedical MRs requires real-time feedback on their position and configuration. Ultrasound (US) imaging stands as a mature and advantageous technology for MRs tracking, but it suffers from disturbances due to low contrast resolution. To overcome these limitations and make US imaging suitable for monitoring and tracking MRs, we propose a US contrast enhancement mechanism for MR visualization in echogenic backgrounds (e.g., tissue). Our technique exploits the specific acoustic phase modulation produced by the MR characteristic motions. By applying this principle, we performed real-time visualization and position tracking of a magnetic MR rolling on a lumen boundary, both in static flow and opposing flow conditions, with an average error of 0.25 body-lengths. Overall, the reported results unveil countless possibilities to exploit the proposed approach as a robust feedback strategy for monitoring and tracking biomedical MRs in-vivo.

scholarly journals Dynamic tracking of a magnetic micro-roller using ultrasound phase analysis

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Stefano Pane ◽  
Veronica Iacovacci ◽  
Mohammad Hasan Dad Ansari ◽  
Arianna Menciassi
Keyword(s):  
Real Time ◽  
Average Error ◽  
Contrast Resolution ◽  
Low Contrast ◽  
Non Invasive ◽  
Dynamic Tracking ◽  
Fine Control ◽  
Real Time Visualization ◽  
Acoustic Phase

AbstractMicrorobots (MRs) have attracted significant interest for their potentialities in diagnosis and non-invasive intervention in hard-to-reach body areas. Fine control of biomedical MRs requires real-time feedback on their position and configuration. Ultrasound (US) imaging stands as a mature and advantageous technology for MRs tracking, but it suffers from disturbances due to low contrast resolution. To overcome these limitations and make US imaging suitable for monitoring and tracking MRs, we propose a US contrast enhancement mechanism for MR visualization in echogenic backgrounds (e.g., tissue). Our technique exploits the specific acoustic phase modulation produced by the MR characteristic motions. By applying this principle, we performed real-time visualization and position tracking of a magnetic MR rolling on a lumen boundary, both in static flow and opposing flow conditions, with an average error of 0.25 body-lengths. Overall, the reported results unveil countless possibilities to exploit the proposed approach as a robust feedback strategy for monitoring and tracking biomedical MRs in-vivo.

Real-Time Visualization and Quantification of Oncolytic M1 Virus In Vitro and In Vivo

2021 ◽  
Vol 32 (3-4) ◽  
pp. 158-165
Author(s):  
Wenbo Zhu ◽  
Jiankai Liang ◽  
Jingyi Tan ◽  
Li Guo ◽  
Jing Cai ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Visualization

scholarly journals Ultrasound-guided navigation of a magnetic microrobot using acoustic phase analysis

2021 ◽  
Author(s):  
Stefano Pane ◽  
Veronica Iacovacci ◽  
Mohammad Hasan Dad Ansari ◽  
Arianna Menciassi
Keyword(s):  
Real Time ◽  
Closed Loop ◽  
Average Error ◽  
Low Contrast ◽  
Loop Control ◽  
Non Invasive ◽  
Fine Control ◽  
Real Time Visualization ◽  
Acoustic Phase

Abstract Microrobots (MRs) have attracted significant interest for their potentialities in diagnosis and non-invasive intervention in hard-to-reach body areas. Fine control of biomedical MRs requires real-time feedback on their position and configuration. Ultrasound (US) imaging stands as a mature and advantageous technology for MRs tracking, but it suffers from disturbances due to low contrast resolution. To overcome these limitations and make US imaging suitable for closed-loop MR control, we propose a US contrast enhancement mechanism for MR visualization in heterogeneous and dynamic backgrounds (e.g., tissue). Our technique exploits the specific acoustic phase modulation produced by the MR characteristic motions. By applying this principle, we performed real-time visualization and position tracking of a magnetic MR rolling on a lumen boundary, both in static flow and opposing flow conditions, with an average error of 0.25 body-lengths. Overall, the reported results unveil countless possibilities to exploit the proposed approach as a robust feedback strategy for closed-loop control of medical MRs in-vivo.

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