Genetic Diversity of Staphylococcus Saprophyticus Strains Causing Urinary Tract Infections in Lagos Metropolis

Abstract ObjectiveStaphylococcus saprophyticus is a Gram-positive bacterium implicated in urinary tract infections in sexually active women along with other bacteria. There is a special need to accurately isolate and identify this organism in clinical specimens in order to prevent misidentification in the laboratory. The present study was carried out to evaluate the genetic diversity of one hundred different Staphylococcus saprophyticus strains isolated from women presenting with urinary tract infections reporting in four government hospitals in Lagos Metropolis using 16SrRNA gene sequence analysis. ResultsThe PCR-amplified and sequenced 16SrRNA gene of 100 isolates and confirmed 88 strains of S. saprophyticus to subspecies level, while 8 belong to other genera and 4 could not be ascertained due to low significant similarity. Phylogenetic analysis showed the interrelationship between the isolates from different hospitals. The results showed the distribution of the isolates into two broad clusters and twenty-two sub-clusters. The results revealed the genetic diversity of Staphylococcus saprophyticus isolated from women with urinary tract infections in Lagos Metropolis using 16SrRNA gene sequence.

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Genetic Diversity of Staphylococcus Saprophyticus Strains Causing Urinary Tract Infections in Lagos Metropolis

10.21203/rs.3.rs-117287/v1 ◽

2020 ◽

Author(s):

Felix Oluwasegun Alao ◽

Stella Ifeanyi Smith ◽

Emmanuel Adedayo Omonigbehin ◽

Isaac Adeyemi Adeleye

Keyword(s):

Genetic Diversity ◽

Urinary Tract ◽

Urinary Tract Infections ◽

Gene Sequence ◽

Significant Similarity ◽

Staphylococcus Saprophyticus ◽

Subspecies Level ◽

Gene Sequence Analysis ◽

Tract Infections ◽

16Srrna Gene

Abstract Background: Staphylococcus saprophyticus is a Gram-positive bacterium implicated in urinary tract infections in sexually active women along with other bacteria. There is a special need to accurately isolate and identify this organism in clinical specimens in order to prevent misidentification in the laboratory. The present study was carried out to evaluate the genetic diversity of one hundred different Staphylococcus saprophyticus strains isolated from women presenting with urinary tract infections reporting in four government hospitals in Lagos Metropolis using 16SrRNA gene sequence analysis. Results: The PCR-amplified and sequenced 16SrRNA gene of 100 isolates and confirmed 88 strains of S. saprophyticus to subspecies level, while 8 belong to other genera and 4 could not be ascertained due to low significant similarity. Phylogenetic analysis showed the interrelationship between the isolates from different hospitals. The results showed the distribution of the isolates into two broad clusters and twenty-two sub-clusters. Conclusion: The results revealed the genetic diversity of Staphylococcus saprophyticus isolated from women with urinary tract infections in Lagos Metropolis using 16SrRNA gene sequence.

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Corynebacterium riegelii sp. nov., an Unusual Species Isolated from Female Patients with Urinary Tract Infections

Journal of Clinical Microbiology ◽

10.1128/jcm.36.3.624-627.1998 ◽

1998 ◽

Vol 36 (3) ◽

pp. 624-627 ◽

Cited By ~ 26

Author(s):

Guido Funke ◽

Paul A. Lawson ◽

Matthew D. Collins

Keyword(s):

Urinary Tract ◽

Urinary Tract Infections ◽

Type Strain ◽

Urease Activity ◽

Gene Sequence ◽

Rrna Gene ◽

Rrna Gene Sequence ◽

Coryneform Bacterium ◽

Gene Sequence Analysis ◽

Tract Infections

Four strains of an unknown coryneform bacterium were isolated in pure culture from females with urinary tract infections. Strong urease activity and the ability to slowly ferment maltose but not glucose were the most significant phenotypic features of this catalase-positive, nonmotile, nonlipophilic, rod-shaped bacterium which served to distinguish it from all other presently defined coryneform bacteria. Chemotaxonomic investigations demonstrated that the unknown bacterium belonged to the genus Corynebacterium. Comparative 16S rRNA gene sequence analysis revealed that the isolates were genealogically identical and represented a new subline within the genusCorynebacterium, for which the designationCorynebacterium riegelii sp. nov. is proposed. The type strain of Corynebacterium riegelii is CCUG 38180 (DSM 44326, CIP 105310).

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