Applicability of Human-specific STR systems, GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in Chimpanzee (Pan troglodytes)

Abstract Objectives The human identification systems based on STRs are widely used in human population genetics and forensic analysis. This study aimed to validate the cross-reactivity of three widely known Human-specific STR identification systems i.e. GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in Chimpanzee. Result The present study revealed the successful amplification of 18 loci using GlobalFiler™ PCR Amplification Kit, 18 loci using Investigator 24plex QS Kit, and 20 loci using PowerPlex® Fusion 6C system. The marker Amelogenin (AMEL) showed differential allele size between male and female revealing the gender identity of Chimpanzees and thus validates their application concerning forensic examination, population estimation, and genetic analysis.

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Applicability of human-specific STR systems, GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee (Pan troglodytes)

BMC Research Notes ◽

10.1186/s13104-021-05632-6 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Abhishek Singh ◽

Vivek Sahajpal ◽

Mukesh Thakur ◽

Lalit Kumar Sharma ◽

Kailash Chandra ◽

...

Keyword(s):

Population Genetics ◽

Pan Troglodytes ◽

Human Population ◽

Pcr Amplification ◽

Forensic Analysis ◽

Cross Reactivity ◽

Allele Size ◽

Male And Female ◽

Successful Amplification ◽

Human Specific

Abstract Objectives Human identification systems based on STRs are widely used in human population genetics and forensic analysis. This study aimed to validate the cross-reactivity of three widely known human-specific STR identification systems i.e. GlobalFiler™ PCR Amplification Kit, Investigator 24plex QS Kit, and PowerPlex® Fusion 6C in chimpanzee. Results The present study revealed the successful amplification of 18 loci using GlobalFiler™ PCR Amplification Kit, 18 loci using Investigator 24plex QS Kit, and 20 loci using PowerPlex® Fusion 6C system. The marker Amelogenin (AMEL) showed differential allele size between male and female revealing the gender identity of chimpanzees and thus validates their application concerning forensic examination, population estimation, and genetic analysis.

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Cross-species validation of human specific STR system, SureID® 21G and SureID® 23comp (Health Gene Technologies) in Chimpanzee (Pan Troglodytes)

BMC Research Notes ◽

10.1186/s13104-019-4801-3 ◽

2019 ◽

Vol 12 (1) ◽

Cited By ~ 1

Author(s):

Abhishek Singh ◽

Mukesh Thakur ◽

Vivek Sahajpal ◽

Sujeet K. Singh ◽

Kailash Chandra ◽

...

Keyword(s):

Pan Troglodytes ◽

Population Genetic ◽

Paternity Testing ◽

Genetic Studies ◽

Banding Patterns ◽

Male And Female ◽

Test Cross ◽

Gene Technologies ◽

Str System ◽

Human Specific

Abstract Objectives The human specific commercially available STRs system are often not tested in non human primates for their cross applicability. The aim of this study is to test Cross-species validation of two commercially available human specific STR kits i.e. SureID® 21G and SureID® 23comp (Health Gene Technologies) for their positive application in Chimpanzee (Pan troglodytes). Result In SureID® 21G, 19 loci amplified and while 20 loci amplified in SureID® 23comp. All the amplified loci in both STR kits were found polymorphic and the locus Amelogenin showed differential banding patterns between male and female revealing their known gender. The present study validates the applicability of these human specific STR kits in Chimpanzee that can be used in forensics analysis, paternity testing and population genetic studies.

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Isolates and Crosses in Human Population Genetics; or, A Contextualization of German Race Science

Current Anthropology ◽

10.1086/662574 ◽

2012 ◽

Vol 53 (S5) ◽

pp. S69-S82 ◽

Cited By ~ 29

Author(s):

Veronika Lipphardt

Keyword(s):

Population Genetics ◽

Human Population ◽

Human Population Genetics ◽

Race Science

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Immunocytochemical demonstration of a SALMFamide-like neuropeptide in the nervous system of adult and larval stages of the human blood fluke, Schistosoma mansoni

Parasitology ◽

10.1017/s0031182000063903 ◽

1995 ◽

Vol 110 (2) ◽

pp. 143-153 ◽

Cited By ~ 7

Author(s):

D. J. A. Brownlee ◽

I. Fairweather ◽

C. F. Johnston ◽

M. C. Thorndyke ◽

P. J. Skuce

Keyword(s):

Nervous System ◽

Schistosoma Mansoni ◽

Cross Reactivity ◽

Confocal Scanning Laser Microscopy ◽

Male Worm ◽

Male And Female ◽

Larval Stages ◽

Confocal Scanning ◽

Confocal Scanning Laser ◽

Nerve Cords

SUMMARYThe localization and distribution of SALMFamide immunoreactivity (IR), SI(GFNSALMFamide), in the nervous system of both the adult and larval stages of the trematode Schistosoma mansoni has been determined by an indirect immunofluorescent technique in conjunction with confocal scanning laser microscopy (CSLM). Immunostaining was widespread in the nervous system of adult male and female S. mansoni. In the central nervous system (CNS), IR was evident in nerve cells and fibres in the anterior ganglia, cerebral commissure and dorsal and ventral nerve cords. In the peripheral nervous system (PNS), IR was apparent in nerve plexuses associated with the subtegmental musculature, oral and ventral suckers, the lining of the gynaecophoric canal, and in fine nerve fibres innervating the dorsal tubercles of the male worm. In the reproductive system of male and female worms, Sl-IR was only observed around the ootype/Mehlis' gland complex in the female. Immunostaining was also evident in the nervous system of both miracidium and cercarial larval stages. A post-embedding, IgG-conjugated colloidal gold immunostaining technique was employed to examine the subcellular distribution of SALMFamide-IR in the CNS of S. mansoni. Gold labelling of peptide was localized over dense-cored vesicles within nerve cell bodies and fibres constituting the neuropile of the anterior ganglia, cerebral commissure and nerve cords of the CNS. Antigen pre-absorption studies indicated that the results obtained do suggest S1-like immunostaining and not cross-reactivity with other peptides, in particular FMRFamide.

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Purity and Mixture in Human Population Genetics

Degrees of Mixture, Degrees of Freedom ◽

10.1215/9780822373070-002 ◽

2017 ◽

pp. 27-52

Keyword(s):

Population Genetics ◽

Human Population ◽

Human Population Genetics

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Acknowledgement to Reviewers of Human Population Genetics and Genomics

10.47248/hpgg2101010006 ◽

2021 ◽

pp. 1-1

Keyword(s):

Population Genetics ◽

Human Population ◽

Human Population Genetics ◽

Genetics And Genomics

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Selection and Identification of Novel Aptamers Specific for Clenbuterol Based on ssDNA Library Immobilized SELEX and Gold Nanoparticles Biosensor

Molecules ◽

10.3390/molecules23092337 ◽

2018 ◽

Vol 23 (9) ◽

pp. 2337 ◽

Cited By ~ 4

Author(s):

Xixia Liu ◽

Qi Lu ◽

Sirui Chen ◽

Fang Wang ◽

Jianjun Hou ◽

...

Keyword(s):

Gold Nanoparticles ◽

High Throughput ◽

High Throughput Sequencing ◽

Limit Of Detection ◽

Retention Rate ◽

Pcr Amplification ◽

Cross Reactivity ◽

Enriched Library ◽

Amplification Curve ◽

Specificity And Sensitivity

We describe a multiple combined strategy to discover novel aptamers specific for clenbuterol (CBL). An immobilized ssDNA library was used for the selection of specific aptamers using the systematic evolution of ligands by exponential enrichment (SELEX). Progress was monitored using real-time quantitative PCR (Q-PCR), and the enriched library was sequenced by high-throughput sequencing. Candidate aptamers were picked and preliminarily identified using a gold nanoparticles (AuNPs) biosensor. Bioactive aptamers were characterized for affinity, circular dichroism (CD), specificity and sensitivity. The Q-PCR amplification curve increased and the retention rate was about 1% at the eighth round. Use of the AuNPs biosensor and CD analyses determined that six aptamers had binding activity. Affinity analysis showed that aptamer 47 had the highest affinity (Kd = 42.17 ± 8.98 nM) with no cross reactivity to CBL analogs. Indirect competitive enzyme linked aptamer assay (IC-ELAA) based on a 5′-biotin aptamer 47 indicated the limit of detection (LOD) was 0.18 ± 0.02 ng/L (n = 3), and it was used to detect pork samples with a mean recovery of 83.33–97.03%. This is the first report of a universal strategy including library fixation, Q-PCR monitoring, high-throughput sequencing, and AuNPs biosensor identification to select aptamers specific for small molecules.

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