Genetic Analysis of Porcine Parvoviruses Detected in South Korean Wild Boars

Abstract Porcine parvovirus 1 (PPV1) is a major cause of reproductive failure in pigs; to date, six novel porcine parvoviruses (PPV2–PPV7) have been identified. Here, we detected 11 PPV1, five PPV3, three PPV4, six PPV5, five PPV6, and one PPV7 strain in Korean wild boars. PPV1, -3, and -5, and PPV6, from Korean wild boars harbor conserved motifs within the Ca2+ binding loop and the catalytic center of the PLA1 motif. Intra-recombination among PPV7 strains was also identified. Genetic characterization revealed that PPV1 from Korean wild boars may be similar to virulent PPV strains.

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Reproductive failure in wild boars associated to porcine parvovirus infection and in vivo and in vitro characterization of the causal isolate

Tropical Animal Health and Production ◽

10.1007/s11250-010-9644-9 ◽

2010 ◽

Vol 42 (8) ◽

pp. 1611-1613 ◽

Cited By ~ 7

Author(s):

Chao-fan Zhang ◽

Cui-Ping Song ◽

Chang-mu Chen ◽

Shang-jin Cui ◽

Lan-fei Miao

Keyword(s):

Reproductive Failure ◽

Porcine Parvovirus ◽

Wild Boars ◽

In Vitro Characterization

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Genetic analysis of porcine parvoviruses detected in South Korean wild boars

Archives of Virology ◽

10.1007/s00705-021-05106-x ◽

2021 ◽

Author(s):

Gyu-Nam Park ◽

Sok Song ◽

Ra Mi Cha ◽

SeEun Choe ◽

Jihye Shin ◽

...

Keyword(s):

Genetic Analysis ◽

Wild Boars ◽

South Korean

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Rapid Spread of Classical Swine Fever Virus among South Korean Wild Boars in Areas near the Border with North Korea

Pathogens ◽

10.3390/pathogens9040244 ◽

2020 ◽

Vol 9 (4) ◽

pp. 244 ◽

Cited By ~ 4

Author(s):

SeEun Choe ◽

Ra Mi Cha ◽

Dae-Sung Yu ◽

Ki-Sun Kim ◽

Sok Song ◽

...

Keyword(s):

North Korea ◽

Classical Swine Fever ◽

Northern Region ◽

Small Scale ◽

Wild Boars ◽

South Korean ◽

Demilitarized Zone ◽

Positive Antibody ◽

Rapid Spread ◽

Spatial Cluster Analysis

There has been a rapid increase in the number of classical swine fever (CSF) sero-positive wild boars captured near the demilitarized zone (DMZ), located the border with North Korea. In 2015–2016, few CSFV-positive antibody boars were detected; however, the number has increased steeply since 2017. Most occurred in the northern region of Gyeonggi before spreading slowly to Gangwon (west to east) in 2018–2019. Multi-distance spatial cluster analysis provided an indirect estimate of the time taken for CSFV to spread among wild boars: 46.7, 2.6, and 2.49 days/km. The average CSF serum neutralization antibody titer was 4–10 (log 2), and CSFV Ab B-ELISA PI values ranged from 65.5 to 111.5, regardless of the age and sex of wild boars. Full genome analysis revealed that 16 CSFV strains isolated from wild boars between 2017 and 2019 were identical to the YC16CS strain (sub-genotype 2.1d) isolated from an outbreak in breeding pigs near the border with North Korea in 2016. The rapid increase in CSF in wild boars may be due to a continuously circulating infection within hub area and increased population density. The distribution pattern of CSFV in Korean wild boars moves from west to southeast, affected by external factors, including small-scale hunting, geographical features and highways.

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Reproductive failure associated with porcine parvovirus in an enzootically infected pig herd

Veterinary Record ◽

10.1136/vr.131.22.503 ◽

1992 ◽

Vol 131 (22) ◽

pp. 503-506 ◽

Cited By ~ 6

Author(s):

C. Huysman ◽

L. van Leengoed ◽

M. de Jong ◽

A. van Osta

Keyword(s):

Reproductive Failure ◽

Porcine Parvovirus

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Porcine Parvovirus Infection Causes Pig Placenta Tissue Damage Involving Nonstructural Protein 1 (NS1)-Induced Intrinsic ROS/Mitochondria-Mediated Apoptosis

Viruses ◽

10.3390/v11040389 ◽

2019 ◽

Vol 11 (4) ◽

pp. 389 ◽

Cited By ~ 6

Author(s):

Zhang ◽

Fan ◽

Li ◽

Liang ◽

Huo ◽

...

Keyword(s):

Host Cell ◽

Cell Apoptosis ◽

Nonstructural Protein ◽

Placental Tissue ◽

Reproductive Failure ◽

Porcine Parvovirus ◽

Nonstructural Protein 1 ◽

Host Cell Apoptosis ◽

Tissue Damages ◽

Induced Apoptosis

Porcine parvovirus (PPV) is an important pathogen causing reproductive failure in pigs. PPV-induced cell apoptosis has been recently identified as being involved in PPV-induced placental tissue damages resulting in reproductive failure. However, the molecular mechanism was not fully elucidated. Here we demonstrate that PPV nonstructural protein 1 (NS1) can induce host cell apoptosis and death, thereby indicating the NS1 may play a crucial role in PPV-induced placental tissue damages and reproductive failure. We have found that NS1-induced apoptosis was significantly inhibited by caspase 9 inhibitor, but not caspase 8 inhibitor, and transfection of NS1 gene into PK-15 cells significantly inhibited mitochondria-associated antiapoptotic molecules Bcl-2 and Mcl-1 expressions and enhanced proapoptotic molecules Bax, P21, and P53 expressions, suggesting that NS1-induced apoptosis is mainly through the mitochondria-mediated intrinsic apoptosis pathway. We also found that both PPV infection and NS1 vector transfection could cause host DNA damage resulting in cell cycle arrest at the G1 and G2 phases, trigger mitochondrial ROS accumulation resulting in mitochondria damage, and therefore, induce the host cell apoptosis. This study provides a molecular basis for elucidating PPV-induced cell apoptosis and reproductive failure.

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Genetic characterization of porcine circovirus type 2 in captive wild boars in southern Brazil

Tropical Animal Health and Production ◽

10.1007/s11250-017-1282-z ◽

2017 ◽

Vol 49 (5) ◽

pp. 1071-1075 ◽

Cited By ~ 1

Author(s):

José Paulo Hiroji Sato ◽

Danielle Gava ◽

Rejane Schaefer ◽

Maurício Egídio Cantão ◽

Janice Reis Ciacci-Zanella ◽

...

Keyword(s):

Genetic Characterization ◽

Porcine Circovirus Type ◽

Porcine Circovirus Type 2 ◽

Porcine Circovirus ◽

Southern Brazil ◽

Wild Boars

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Phylogeny and evolutionary genetics of porcine parvovirus in wild boars

Infection Genetics and Evolution ◽

10.1016/j.meegid.2012.04.020 ◽

2012 ◽

Vol 12 (6) ◽

pp. 1163-1171 ◽

Cited By ~ 19

Author(s):

Dániel Cadar ◽

Ádám Dán ◽

Kata Tombácz ◽

Márta Lőrincz ◽

Timea Kiss ◽

...

Keyword(s):

Evolutionary Genetics ◽

Porcine Parvovirus ◽

Wild Boars

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Genetic characterization of pea (Pisum sativum L.) mutants P59 and P60, defective in nitrogen fixation

Abstract book of the 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology" PLAMIC2020 ◽

10.28983/plamic2020.122 ◽

2020 ◽

Author(s):

O. P. Klimenko ◽

O. A. Kulaeva ◽

O. Y. Shtark ◽

A. I. Zhernakov ◽

I. A. Tikhonovich ◽

...

Keyword(s):

Nitrogen Fixation ◽

Pisum Sativum ◽

Genetic Analysis ◽

Genetic Characterization ◽

Symbiotic Genes ◽

Pisum Sativum L ◽

Pea Mutants ◽

First Results

Several genes involved in development of symbiosis between pea and rhizobia haven’t yet been characterized in detail. Here, the first results of genetic analysis of pea mutants in the symbiotic genes Sym23 and Sym24 are presented.

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Prevalence and Genetic Diversity of Atypical Porcine Pestivirus (APPV) Detected in South Korean Wild Boars

Viruses ◽

10.3390/v12060680 ◽

2020 ◽

Vol 12 (6) ◽

pp. 680 ◽

Cited By ~ 2

Author(s):

SeEun Choe ◽

Gyu-Nam Park ◽

Ra Mi Cha ◽

Bang-Hun Hyun ◽

Bong-Kyun Park ◽

...

Keyword(s):

Maximum Likelihood Method ◽

Likelihood Method ◽

Structural Protein ◽

Nonstructural Proteins ◽

Domestic Pig ◽

Tree Analysis ◽

Wild Boars ◽

South Korean ◽

Congenital Tremor ◽

Reference Strains

Atypical porcine pestivirus (APPV), currently classified as pestivirus K, causes congenital tremor (CT) type A-II in piglets. Eighteen APPV strains were identified from 2297 South Korean wild boars captured in 2019. Phylogenetic analysis of the structural protein E2 and nonstructural proteins NS3 and Npro classified the APPV viruses, including reference strains, into Clades I, II and III. Clade I was divided into four subclades; however, the strains belonging to the four subclades differed slightly, depending on the tree analysis, the NS3, E2, and Npro genes. The maximum-likelihood method was assigned to South Korean wild boar APPV strains to various subclades within the three trees: subclades I.1 and I.2 in the E2 tree, subclade I.1 in the Npro tree, and subclades I.1 and I.4 in the NS3 ML tree. In conclusion, APPV among South Korean wild boars belonging to Clade I may be circulating at a higher level than among the South Korean domestic pig populations.

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