scholarly journals A Simplified Procedures for Cellulase Filter Paper Assay

2021 ◽  
Author(s):  
Mohamed Abdelazim Abulela
Keyword(s):  
Optical Density ◽  
Filter Paper ◽  
Main Idea ◽  
Activity Measurement ◽  
International Union ◽  
Absorbance Difference ◽  
Simplified Procedures ◽  
New Procedures ◽  
Filter Paper Assay ◽  
Filter Paper Activity

Abstract A new procedures to minimize labor intensiveness and complexity that has long been recognized in cellulase filter paper activity measurement (FPAase) described by the international union of pure and applied chemistry (IUPAC) was developed. It follows the main idea of IUPAC with only exception finding at least two cellulase dilutions have optical densities slightly more and less than a reference optical density of an arbitrary fixed 2 mg absolute glucose amount after a red-ox color reaction and due to cellulase-filter paper hydrolysis. The yielding glucose amount difference as compared to this reference is expressed in terms of absorbance difference percent determined by 3,5-dinitrosalicylic acid (DNS), in case of cellulase is cellubiase rich. If not, an external supplemental portion should be added. The intersection of the line of these two cellulase dilutions with abscissa intersect a vertical at a hypothetical 0% absorbance difference percent corresponding to a critical cellulase dilution exactly release this fixed arbitrary 2 mg glucose amount value. The factor 0.37 of this critical cellulase dilution equals to its filter paper units expressed in FPU per ml.

scholarly journals A Simplified Procedures for Cellulase Filter Paper Assay

2021 ◽  
Author(s):  
Mohamed Abdelazim Abulela
Keyword(s):  
Optical Density ◽  
Filter Paper ◽  
Main Idea ◽  
Activity Measurement ◽  
International Union ◽  
Absorbance Difference ◽  
Simplified Procedures ◽  
New Procedures ◽  
Filter Paper Assay ◽  
Filter Paper Activity

<p>A new procedures to minimize labor intensiveness and complexity that has long been recognized in cellulase filter paper activity measurement (FPAase) described by the international union of pure and applied chemistry (IUPAC)was developed. It follows the main idea of IUPAC with only exception finding at least two cellulase dilutions have optical densities slightly more and less than a reference optical density of an arbitrary fixed 2 mg absolute glucose amount after a red-ox color reaction and due to cellulase-filter paper hydrolysis. The yielding glucose amount difference as compared to this reference is expressed in terms of absorbance difference percent determined by 3,5-dinitrosalicylic acid (DNS), in case of cellulase is cellubiase rich. If not, an external supplemental portion should be added. The intersection of the line of these two cellulase dilutions with abscissa intersect a vertical at a hypothetical 0% absorbance difference percent corresponding to a critical cellulase dilution exactly release this fixed arbitrary 2 mg glucose amount value. The factor 0.37 of this critical cellulase dilution equals to its filter paper units expressed in FPU per ml.</p><br>

scholarly journals A Simplified Procedures for Cellulase Filter Paper Assay

2021 ◽  
Author(s):  
Mohamed Abdelazim Abulela
Keyword(s):  
Optical Density ◽  
Filter Paper ◽  
Main Idea ◽  
Activity Measurement ◽  
International Union ◽  
Absorbance Difference ◽  
Simplified Procedures ◽  
New Procedures ◽  
Filter Paper Assay ◽  
Filter Paper Activity

<p>A new procedures to minimize labor intensiveness and complexity that has long been recognized in cellulase filter paper activity measurement (FPAase) described by the international union of pure and applied chemistry (IUPAC)was developed. It follows the main idea of IUPAC with only exception finding at least two cellulase dilutions have optical densities slightly more and less than a reference optical density of an arbitrary fixed 2 mg absolute glucose amount after a red-ox color reaction and due to cellulase-filter paper hydrolysis. The yielding glucose amount difference as compared to this reference is expressed in terms of absorbance difference percent determined by 3,5-dinitrosalicylic acid (DNS), in case of cellulase is cellubiase rich. If not, an external supplemental portion should be added. The intersection of the line of these two cellulase dilutions with abscissa intersect a vertical at a hypothetical 0% absorbance difference percent corresponding to a critical cellulase dilution exactly release this fixed arbitrary 2 mg glucose amount value. The factor 0.37 of this critical cellulase dilution equals to its filter paper units expressed in FPU per ml.</p><br>

scholarly journals A Simplified Procedure for Cellulase Filter Paper Assay

2021 ◽  
pp. 60-64
Author(s):  
Mohamed Abdelazim
Keyword(s):  
Optical Density ◽  
Filter Paper ◽  
Main Idea ◽  
Activity Measurement ◽  
International Union ◽  
Absorbance Difference ◽  
Simplified Procedure ◽  
New Procedures ◽  
Filter Paper Assay ◽  
Filter Paper Activity

A new procedures to minimize labor intensiveness and complexity that has long been recognized in cellulase filter paper activity measurement (FPAase) described by the international union of pure and applied chemistry (IUPAC) was developed. It follows the main idea of IUPAC finding at least two cellulase dilutions have optical densities slightly more and less than a reference optical density of an arbitrary fixed 2 mg absolute glucose amount after a red-ox color reaction and due to cellulase-filter paper hydrolysis. The yielding glucose amount difference as compared to this reference is expressed in terms of absorbance difference percent determined by 3,5-dinitrosalicylic acid (DNS), in case of cellulase is cellubiase rich. If not, an external supplemental portion should be added. The intersection of the line of these two cellulase dilutions with abscissa intersect a vertical at a hypothetical 0% absorbance difference percent corresponding to a critical cellulase dilution exactly release this fixed arbitrary 2 mg glucose amount value. The factor 0.37 of this critical cellulase dilution equals to its filter paper units expressed in FPU per ml. A cellulase Cellic Ctech2 from novozymes has been tested with this methodology giving a satisfactory results with IUPAC procedures.

The Influence of PH and Dissolved Oxygen Tension (DOT) on Mycelium Growth and Cellulase Production by Trichoderma Reesei

2011 ◽  
Vol 236-238 ◽  
pp. 1005-1013
Author(s):  
Zhi Xi Hang ◽  
Qing Long Rao ◽  
Shi Yuan Yu
Keyword(s):  
Dissolved Oxygen ◽  
Trichoderma Reesei ◽  
Oxygen Tension ◽  
Filter Paper ◽  
Mass Concentration ◽  
Cellulase Production ◽  
Mycelium Growth ◽  
Dissolved Oxygen Tension ◽  
Influence Of Ph ◽  
Filter Paper Activity

The influence of pH and dissolved oxygen tension (DOT) on mycelium growth and cellulase production by Trichoderma reesei was studied in this paper. The experiments were carried out with a cellulose of 10 g/l in a 10 L steam sterilizable bioreactor. The results have shown that H+ concentration was highly fluctuated in the growing and metabolizing periods of mycelium, which went against mycelium growth and cellulase production. Controlling pH to 4.8 was favorable to mycelium growth and cellulase production; the maximum mycelium mass concentration was increased from 2.60 g/l to 2.77 g/l; the maximum filter paper activity was raised from 1.87 IU/ml to 2.79 IU/ml. Meanwhile, the growth and metabolism of mycelium demand an appropriate dissolved oxygen tension (DOT). When the velocity of aeration was increased from 0.4 to 0.5vvm to improve the condition of dissolving oxygen, the mycelium mass concentration was increased from 2.77 g/l to 2.98g/l, and the filter paper activity was raised from 2.79 IU/ml to 2.98 IU/ml.

scholarly journals Morphogenesis and Production of Enzymes byPenicillium echinulatumin Response to Different Carbon Sources

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Willian Daniel Hahn Schneider ◽  
Laísa dos Reis ◽  
Marli Camassola ◽  
Aldo José Pinheiro Dillon
Keyword(s):  
Enzymatic Activity ◽  
Sugar Cane ◽  
Filter Paper ◽  
Carbon Sources ◽  
Sugar Cane Bagasse ◽  
Xylanase Production ◽  
Penicillium Echinulatum ◽  
The Media ◽  
Filter Paper Activity ◽  
Second Generation Ethanol

The effect of different carbon sources on morphology and cellulase and xylanase production ofPenicillium echinulatumwas evaluated in this work. Among the six carbon sources studied, cellulose and sugar cane bagasse were the most suitable for the production of filter paper activity, endoglucanases, xylanases, andβ-glucosidases. However, sucrose and glucose showedβ-glucosidase activities similar to those obtained with the insoluble sources. The polyacrylamide gels proved the enzymatic activity, since different standards bands were detected in the media mentioned above. Regarding morphology, it was observed that the mycelium in a dispersed form provided the greatest enzymatic activity, possibly due to greater interaction between the substrate and hyphae. These data are important in understanding the physiology of fungi and could contribute to obtaining enzyme with potential application in the technology of second generation ethanol.

Toxicity of Carbaryl toward the Southern Pine Beetle in Filter Paper, Bark and Cut Bolt Bioassays

1994 ◽  
Vol 29 (2) ◽  
pp. 247-253 ◽  
Author(s):  
He Zhong ◽  
Felton L. Hastings ◽  
Fred P. Hain ◽  
Richard A. Werner
Keyword(s):  
Loblolly Pine ◽  
Filter Paper ◽  
Untreated Control ◽  
Dendroctonus Frontalis ◽  
Southern Pine Beetle ◽  
Southern Pine ◽  
Lc50 Value ◽  
Pine Beetle ◽  
Filter Paper Assay

Loblolly pine bolts sprayed with 2% carbaryl (Sevimol® 40% A. I. Flowable) and 1% chlorpyrifos were exposed to southern pine beetle, Dendroctonus frontalis Zimmermann. Chlorpyrifos treatment effectively prevented attack by southern pine beetle; however, the bolts were not protected by 2% carbaryl. For the carbaryl-treated bolts, the number of egg niches and larval mines were significantly reduced. However, the number of adult beetles entering the host, the length of the parent galleries, the number of pupal chambers, brood adults, and emergence holes were not significantly (P = 0.05) different from untreated control bolts. A bark surface assay indicated that 2% carbaryl killed &lt; 50% of the beetles at 24 h. Filter paper assay showed that the LC50 value was 0.07% and 0.01% at 24 and 48 h, respectively.

Suggested improvements to the standard filter paper assay used to measure cellulase activity

2003 ◽  
Vol 82 (6) ◽  
pp. 745-749 ◽  
Author(s):  
Guillermo Coward-Kelly ◽  
Cateryna Aiello-Mazzari ◽  
Sehoon Kim ◽  
Cesar Granda ◽  
Mark Holtzapple
Keyword(s):  
Filter Paper ◽  
Cellulase Activity ◽  
Filter Paper Assay
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