Identification microflora related to growth performance in pigs based on 16s rRNA sequence analyses

Abstract Intestinal microorganisms have been shown to be important factors that affect the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n = 10), Landrace (n = 9) and Yorkshire (n = 21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via 16S ribosomal RNA (rRNA) gene sequencing. And we also analyzed their growth performance. We found that Duroc and Landrace had significant differences in average daily gain (ADG), feed efficiency ratio (FER), growth index (GI), and 100 kg daily age (P < 0.05). Moreover, through the analysis of the intestinal flora, we also identified that there were 18 species of intestinal flora with significant differences between Duroc and Landrace (P < 0.05). In order to eliminate the influence of genetic background, the intestinal differential flora of 21 Yorkshire pigs with different growth performance was analyzed. The results showed that there were significant correlations between Barnesiella, Dorea, Clostridium and Lactobacillus and pig growth performance. To explore the effect of intestinal flora on the growth performance of pigs at the molecular level, Lactobacillus, which has the highest content in the intestine, was selected for isolation and purification, and co-cultured with intestinal epithelial cells. The qPCR was used to determine the effect of Lactobacillus on MC4R gene expression in intestinal epithelial cells. The results showed that Lactobacillus inhibited MC4R gene expression in intestinal epithelial cells. The results provided useful reference for the further study on the relationship between intestinal flora and pig growth performance.

Download Full-text

Identification microflora related to growth performance in pigs based on 16s rRNA sequence analyses

10.21203/rs.3.rs-62183/v2 ◽

2020 ◽

Author(s):

Xin-Jian Li ◽

Mingyu Wang ◽

Yahui Xue ◽

Dongdong Duan ◽

Cong Li ◽

...

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Growth Performance ◽

Intestinal Epithelial Cells ◽

Average Daily Gain ◽

Intestinal Flora ◽

Rrna Gene ◽

Intestinal Epithelial ◽

Microbial Composition ◽

Mc4r Gene

Abstract Intestinal microorganisms have been shown to be important factors that affect the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n=10), Landrace (n=9) and Yorkshire (n=21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via 16S ribosomal RNA (rRNA) gene sequencing. And we also analyzed their growth performance. We found that Duroc and Landrace had significant differences in average daily gain (ADG), feed efficiency ratio (FER), growth index (GI), and 100kg daily age ( P<0.05 ). Moreover, through the analysis of the intestinal flora, we also identified that there were 18 species of intestinal flora with significant differences between Duroc and Landrace ( P<0.05 ). In order to eliminate the influence of genetic background, the intestinal differential flora of 21 Yorkshire pigs with different growth performance was analyzed. The results showed that there were significant correlations between Barnesiella , Dorea , Clostridium and Lactobacillus and pig growth performance. To explore the effect of intestinal flora on the growth performance of pigs at the molecular level, Lactobacillus , which has the highest content in the intestine, was selected for isolation and purification, and co-cultured with intestinal epithelial cells. The qPCR was used to determine the effect of Lactobacillus on MC4R gene expression in intestinal epithelial cells. The results showed that Lactobacillus inhibited MC4R gene expression in intestinal epithelial cells. The results provided useful reference for the further study on the relationship between intestinal flora and pig growth performance.

Download Full-text

Identification of microflora related to growth performance in pigs based on 16S rRNA sequence analyses

AMB Express ◽

10.1186/s13568-020-01130-3 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Xin-Jian Li ◽

Mingyu Wang ◽

Yahui Xue ◽

Dongdong Duan ◽

Cong Li ◽

...

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Growth Performance ◽

Intestinal Epithelial Cells ◽

Average Daily Gain ◽

Intestinal Flora ◽

Rrna Gene ◽

Intestinal Epithelial ◽

Microbial Composition ◽

Mc4r Gene

Abstract Intestinal microorganisms have been shown to be important factors affecting the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n = 10), Landrace (n = 9) and Yorkshire (n = 21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via 16S ribosomal RNA (rRNA) gene sequencing. We also analyzed their growth performance. We found that Duroc and Landrace pigs had significant differences in average daily gain (ADG), feed efficiency ratio (FER), growth index (GI), and number of days taken to reach 100 kg (P < 0.05). Moreover, through analysis of the intestinal flora, we also identified 18 species of intestinal flora with significant differences between Duroc and Landrace pigs (P < 0.05). To eliminate the influence of genetic background, the differential intestinal flora of 21 Yorkshire pigs with differences in growth performance was analyzed. The results showed that there were significant correlations between Barnesiella, Dorea, Clostridium and Lactobacillus and pig growth performance. To explore the effect of the intestinal flora on the growth performance of pigs at the molecular level, Lactobacillus, which is the most abundant in the intestine, was selected for isolation and purification and cocultured with intestinal epithelial cells. qPCR was used to determine the effect of Lactobacillus on MC4R gene expression in intestinal epithelial cells. The results showed that Lactobacillus inhibited MC4R gene expression in these cells. The results provide a useful reference for further study of the relationship between the intestinal flora and pig growth performance.

Download Full-text

Identification of microflora related to growth performance in pigs based on 16S rRNA sequence analyses

10.21203/rs.3.rs-62183/v3 ◽

2020 ◽

Author(s):

Xin-Jian Li ◽

Mingyu Wang ◽

Yahui Xue ◽

Dongdong Duan ◽

Cong Li ◽

...

Keyword(s):

Gene Expression ◽

Epithelial Cells ◽

Growth Performance ◽

Intestinal Epithelial Cells ◽

Average Daily Gain ◽

Intestinal Flora ◽

Rrna Gene ◽

Intestinal Epithelial ◽

Microbial Composition ◽

Mc4r Gene

Abstract Intestinal microorganisms have been shown to be important factors affecting the growth performance of pigs. Therefore, to investigate the effect of the intestinal microflora structure on the growth performance of pigs, samples from Duroc (n=10), Landrace (n=9) and Yorkshire (n=21) pigs under the same diet and feeding conditions were collected. The fecal microbial composition was profiled via 16S ribosomal RNA (rRNA) gene sequencing. We also analyzed their growth performance. We found that Duroc and Landrace pigs had significant differences in average daily gain (ADG), feed efficiency ratio (FER), growth index (GI), and number of days taken to reach 100 kg ( P<0.05 ). Moreover, through analysis of the intestinal flora, we also identified 18 species of intestinal flora with significant differences between Duroc and Landrace pigs ( P<0.05 ). To eliminate the influence of genetic background, the differential intestinal flora of 21 Yorkshire pigs with differences in growth performance was analyzed. The results showed that there were significant correlations between Barnesiella , Dorea , Clostridium and Lactobacillus and pig growth performance. To explore the effect of the intestinal flora on the growth performance of pigs at the molecular level, Lactobacillus , which is the most abundant in the intestine, was selected for isolation and purification and cocultured with intestinal epithelial cells. qPCR was used to determine the effect of Lactobacillus on MC4R gene expression in intestinal epithelial cells. The results showed that Lactobacillus inhibited MC4R gene expression in these cells. The results provide a useful reference for further study of the relationship between the intestinal flora and pig growth performance.

Download Full-text

Characterization and comparison of the bacterial community between complete intensive and extensive feeding patterns in pigs

AMB Express ◽

10.1186/s13568-021-01191-y ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Xin-Jian Li ◽

Mingyu Wang ◽

Yahui Xue ◽

Dongdong Duan ◽

Cong Li ◽

...

Keyword(s):

Gene Expression ◽

Disease Resistance ◽

Epithelial Cells ◽

Intestinal Epithelial Cells ◽

Intestinal Flora ◽

Feeding Pattern ◽

Rrna Gene ◽

Intestinal Epithelial ◽

Microbial Composition ◽

Drb Gene

AbstractTo investigate and compare the gut microbiota structures in complete intensive feeding pattern (CP) and extensive feeding pattern (EP) groups, a total of 20 pigs were divided into two groups and fed the same diet. The fecal microbial composition was profiled using 16S rRNA gene sequencing. Our results showed that seventeen predominant genera were present in each pig sample and constituted the phylogenetic core of the microbiota at the class level. The abundance of most of the core microbial flora were significantly higher in the CP group than in the EP group (P < 0.05), while the abundance of Gammaproteobacteria was significantly lower in the CP group than in the EP group (P < 0.05). The CP group had significantly greater community diversity, richness, and evenness than the EP group (P < 0.05). Functional prediction analysis indicated that intestinal microbial species potentially led to faster growth and an increased fat accumulation capacity in the CP group; however, disease resistance was weaker in the CP group than in the EP group. In conclusion, EP pigs have a wider range of activity and better animal welfare than CP pigs, which helps reduce the occurrence of diseases and neurological symptoms. To explore the effect of intestinal flora on disease resistance in pigs at the molecular level, Coprococcus, which is a key gut bacterium in the intestine, was selected for isolation and purification and cocultured with intestinal epithelial cells. qPCR was performed to determine the effect of Coprococcus on SLA-DRB gene expression in intestinal epithelial cells. The results showed that Coprococcus enhanced SLA-DRB gene expression in intestinal epithelial cells. The results provide useful reference data for further study on the relationship between intestinal flora and pig disease resistance.

Download Full-text

Characterization and comparison of the bacterial community between complete intensive and extensive feeding patterns in pigs

10.21203/rs.3.rs-103593/v1 ◽

2020 ◽

Author(s):

Xin-Jian Li ◽

Mingyu Wang ◽

Yahui Xue ◽

Dongdong Duan ◽

Cong Li ◽

...

Keyword(s):

Gene Expression ◽

Disease Resistance ◽

Epithelial Cells ◽

Intestinal Epithelial Cells ◽

Intestinal Flora ◽

Feeding Pattern ◽

Rrna Gene ◽

Intestinal Epithelial ◽

Microbial Composition ◽

Drb Gene

Abstract To investigate and compare the gut microbiota structure in complete intensive feeding pattern (CP) and extensive feeding pattern (EP) groups, a total of 20 pigs were divided into two groups and fed the same diet. The fecal microbial composition was profiled using 16S rRNA gene sequencing. Our results showed that seventeen predominant genera were present in each pig sample and constituted a phylogenetic core microbiota at the class level. Most of the core microbial flora was significantly higher in the CP group than the EP group ( P < 0.05), while Gammaproteobacteria was significantly lower in the CP group than in the EP group ( P < 0.05). The CP group had significantly higher community diversity, richness, and evenness than the EP group ( P < 0.05). Functional prediction indicated that intestinal microbial species might lead to higher growth and an increased fat accumulation capacity in the CP group; however, disease resistance was weaker in the CP group than the EP group. In conclusion, EP pigs have a wider range of sports venues and better animal welfare than CP pigs, which helps reduce the occurrence of diseases and neurological symptoms. To explore the effect of intestinal flora on the disease resistance of pigs at the molecular level, Coprococcus, which has the key gut bacterium in the intestine, was selected for isolation and purification, and co-cultured with intestinal epithelial cells. The qPCR was used to determine the effect of Coprococcus on SLA-DRB gene expression in intestinal epithelial cells. The results showed that Coprococcus enhance SLA-DRB gene expression in intestinal epithelial cells. The results provided useful references for further study on the relationship between intestinal flora and pig disease resistance.

Download Full-text

Lacticaseibacillus rhamnosus Reduces the Pathogenicity of Escherichia coli in Chickens

Frontiers in Microbiology ◽

10.3389/fmicb.2021.664604 ◽

2021 ◽

Vol 12 ◽

Author(s):

Mengjiao Guo ◽

Congyue Zhang ◽

Chengcheng Zhang ◽

Xiaorong Zhang ◽

Yantao Wu

Keyword(s):

Escherichia Coli ◽

Disease Resistance ◽

Epithelial Cells ◽

Growth Performance ◽

Intestinal Epithelial Cells ◽

Intestinal Flora ◽

Daily Weight Gain ◽

Control Group ◽

Intestinal Epithelial ◽

E Coli

Lacticaseibacillus rhamnosus is a recognized probiotic that is widely used in scientific research and clinical applications. This study found that the Lacticaseibacillus rhamnosus GG (LGG) strain can reduce the adhesion of Escherichia coli (E. coli) to primary chicken intestinal epithelial cells by 75.7% and inhibit 41.7% of the E. coli that adhere to intestinal epithelial cells. Additionally, LGG showed strong inhibitory ability on the growth of E. coli, Staphylococcus aureus, Salmonella Paratyphi B, and Salmonella Enteritidis in vitro. Furthermore, the influence of LGG on the growth performance, intestinal flora, immunity, and disease resistance of chickens was explored. Chickens fed with LGG exhibited increased average daily weight gain and concentrations of sIgA, IgG, and IgM than did controls. After 21 days of feeding, a diet with LGG increased the diversity of intestinal microbiota and maintained intestinal health. Moreover, LGG promoted immunologic barriers by upregulating cytokines and chemokines via the Toll-like receptor. The major pro-inflammatory factors, including Myd88, NF-κB, Il6, and Il8, were upregulated compared to controls. After being challenged with E. coli, the survival rate of chickens fed with LGG was significantly higher than those in the control group, and decreased numbers of E. coli were detected in the heart and lungs of the LGG group. In summary, oral administration of LGG to chickens could improve growth performance, maintain intestinal homeostasis, and enhance innate immune response and disease resistance.

Download Full-text