scholarly journals Assessment of genetic purity in rice using polymorphic SSR markers

2020 ◽  
Author(s):  
S.P. Jeevan Kumar ◽  
C Susmita ◽  
Dinesh K. Agarwal ◽  
Govind Pal ◽  
Abhishek Kumar Rai ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Polymorphic Information Content ◽  
Morphological Characters ◽  
Genetic Purity ◽  
Rice Varieties ◽  
Pcr Products ◽  
Finger Printing ◽  
High Yielding Varieties ◽  
Dna Finger Printing

Abstract Genetic purity is conventionally performed through Grow-Out-Test (GOT) with morphological characters. Simple sequence repeat (SSR) markers are independent of G X E interaction. Herewith, 16 high yielding varieties of rice were analyzed using 55 SSR markers for DNA fingerprinting and identification of genetic impurities: 14 were found to be polypmorphic and amplified 48 alleles with an average of 3.43 alleles per each primer pair. The number of alleles amplified ranged from 2 to 6 and the size of the PCR products amplified from these 14 primer pairs ranged from 80–450 bp with polymorphic information content (PIC) from 0.14 (RM 346) to 0.99 (RM 5900). PICs at 0.5 or higher are highly informative SSR markers for genetic studies, DNA fingerprinting and scoring polymorphism rate of SSR markers pertinent to specific locus. The 14 polymorphic SSR markers can be used for DUS testing, genetic purity and DNA finger printing of rice varieties.

DNA Fingerprinting for Identification of Rice Varieties and Seed Genetic Purity Assessment

2018 ◽  
Vol 7 (4) ◽  
pp. 379-390 ◽  
Author(s):  
Vanisri Satturu ◽  
Durga Rani ◽  
Swathi Gattu ◽  
Jamal Md ◽  
Sreedhar Mulinti ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Genetic Purity ◽  
Rice Varieties ◽  
Purity Assessment

scholarly journals Does monozygotic twinning occur in mice?

1995 ◽  
Vol 66 (3) ◽  
pp. 195-202 ◽  
Author(s):  
Anne McLaren ◽  
Paul Molland ◽  
Esther Signer
Keyword(s):  
Dna Fingerprinting ◽  
Monozygotic Twin ◽  
Statistical Evidence ◽  
Genetic Loci ◽  
Induced Ovulation ◽  
Monozygotic Twinning ◽  
Finger Printing ◽  
Dna Finger Printing ◽  
Mouse System

SummaryPublished reports suggest that the incidence of monozygotic twinning in women is increased after hormonally induced ovulation. Since some statistical evidence exists to indicate that monozygotic twinning may also occur in mice, we attempted to devise a mouse system in which the incidence of such twinning could be compared after spontaneous versus hormonally induced ovulation, in order to analyse the developmental basis of such an effect. We used phenotypic identity in litters segregating for ten genetic loci (not all independent) to indicate possible twin pairs. DNA fingerprinting using three human minisatellite probes was then performed blind on these pairs and on sibling controls. From a total of over 2000 mice born, 40 apparently identical pairs were identified, on which DNA finger-printing was successfully carried out on 35 pairs. All proved to be derived from different zygotes. We conclude that monozygotic twin pairs are either extremely rare in the stock of mice that we studied, or have such reduced viability that their chance of surviving to weaning is low.

DNA fingerprinting and genetic purity testing of a new broccoli hybrid using SSR markers

2013 ◽  
Vol 41 (3) ◽  
pp. 464-468
Author(s):  
H.F. Yu ◽  
J.S. Wang ◽  
Z.Q. Zhao ◽  
X.G. Sheng ◽  
H.H. Gu
Keyword(s):  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Genetic Purity ◽  
Purity Testing

Identification of informative EST-SSR markers capable of distinguishing popular Indian rice varieties and their utilization in seed genetic purity assessments

2011 ◽  
Vol 39 (2) ◽  
pp. 282-292 ◽  
Author(s):  
K. Krishna Moorthy ◽  
Prakash Babu ◽  
M. Sreedhar ◽  
V.S.A.K. Sama ◽  
P. Nataraj Kumar ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Genetic Purity ◽  
Rice Varieties

scholarly journals Characterization of Little millet (Panicum sumatrense) varieties using Morphological descriptors and SSR based DNA fingerprinting

2020 ◽  
pp. 29-34
Author(s):  
Senthil Natesan ◽  
Gayathri Venugopalan ◽  
Selva Babu Selvamani ◽  
Madhumitha Krishnamoorthy ◽  
Sarankumar Chandran ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Pearl Millet ◽  
Ssr Markers ◽  
Morphological Characters ◽  
Dna Fingerprint ◽  
Morphological Descriptors ◽  
Little Millet ◽  
Amplicon Size ◽  
Cereal Genomes ◽  
Unique Markers

Little millet varieties are generally distinguished by morphological descriptors which are being used for seed certification and DUS characterization [1]. But in practical terms, these key differentiation descriptors between varieties of little millet are very fewer and hence difficult to differentiate germplasm accessions. Germplasm registration in NBPGR needs DNA fingerprint to show the uniqueness of germplasm in comparison to existing varieties. DNA fingerprinting is a better option to identify unique markers to differentiate the varieties. Available genomic resources are scarce since little millet is still considered to be an orphan crop. Therefore markers from other cereal genomes such as maize, pearl millet and barnyard millet that are been utilized for DNA fingerprinting purpose with a clue of cereal synteny relationship. Twenty-one morphological descriptors studies revealed that the variety ATL 1 is different from the other varieties for more than 16 morphological characters studied. DNA fingerprinting is attempted in five genotypes of little millets such as BL6, ATL 1, TNPsu 176, Co (Samai) 4, Paiyur 2 using cereal SSR markers. Among the 25 maize SSR markers used two markers viz., phi213984 and phi295450 scored polymorphism by the amplicon size of 310bp and 600bp respectively. From the 25 Pearl millet SSR markers used only one SSR marker found polymorphic at 305bp allele size for ATL 1 and Hence, SSR based DNA fingerprinting helped to differentiate ATL1, the newly released high yielding variety from other genotypes of little millets which can be used for varietal identification purpose.

scholarly journals DNA fingerprinting and genetic diversities in some Bangladeshi aus rice (Oryza sativa L.) genotypes

2017 ◽  
Vol 15 (1) ◽  
pp. 123-137 ◽  
Author(s):  
MM Islam ◽  
HA Begum ◽  
MS Ali ◽  
M Kamruzzaman ◽  
S Hoque ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Oryza Sativa L ◽  
Polymorphic Information Content ◽  
Crop Improvement ◽  
Allelic Diversity ◽  
Breeding Programs ◽  
Chord Distance ◽  
Genetic Dissimilarity

The allelic diversity and relationship among 120 Aus rice landraces were determined through DNA fingerprinting using microsatellite (SSR) markers. A total of 85 SSR markers were used to characterize and discriminate all tested Aus rice genotypes, 45 of which were polymorphic for different chromosome numbers. The number of alleles per locus varied from 6 alleles (RM484 and RM541) to 30 alleles (RM519) with an average of 13 alleles per locus. The polymorphic information content (PIC) values varied ranged from 0.5211 (RM536) to 0.9369 (RM519) with an average 0.8217. The highest PIC value (0.9369) was obtained for RM519 followed by RM286 (0.9357). The genetic distance-based results seen in the unrooted neighbor-joining tree clustering revealed nine genetic groups. Being grouped into distant clusters and with highest genetic distance, eleven genotypes viz., Atithi dhan, Kadar chap, Pankiraj, Japanese-7, Jamri saity, Logi jota, Joba, Lada moni, Manik Mondal-2, Boilum and Brmulka-2 could be selected as potential parents for crop improvement for their distinctive characters. Panchash and Parija had closest distance in the SSR based CS-Chord distance (0.000) might have same genetic background. The highest genetic dissimilarity (1.000) was found among the nineteen Aus genotypes combinations followed by the second highest (0.9778) among 94 Aus rice combinations. Whereas lowest genetic dissimilarity was found between Kala and Kalo Hizli (0.1778) followed by Holat and Holae (0.2667). This information will be useful in the selection of diverse parents, background selection during backcross breeding programs and assist in broadening germplasm-based rice breeding programs in the near future.SAARC J. Agri., 15(1): 123-137 (2017)

scholarly journals Distinguishing Rice Genotypes using Morphological, Agronomical,

2015 ◽  
Vol 34 (2) ◽  
pp. 79 ◽  
Author(s):  
Untung Susanto ◽  
Nofi Anisatun Rohmah ◽  
Made Jana Mejaya
Keyword(s):  
Ssr Markers ◽  
Block Design ◽  
Rice Variety ◽  
Morphological Characters ◽  
Snp Markers ◽  
Complete Data ◽  
Property Right ◽  
Wet Season ◽  
Rice Varieties ◽  
Rice Genotypes

<p align="LEFT">Complete data on characteristics of a rice variety is very important to  race the authenticity of the variety at the field. Sometimes a name of a variety had changed, due to the informal  seed distribution among farmers. This could become problem in the property right of the variety. Distinguishing among rice varieties using only morphological and agronomical traits are sometimes not sufficient. Currently, molecular markers such as SSR (Simple Sequence Repeats) and SNP (Single Nucleotide Polymorphism) markers have become available and are powerfull to distinguish rice genotypes. This research was aimed to distinguish nine rice varieties grown by farmers, using morphological characters (47 traits), agronomical characters (9 traits), SSR markers (12 primer pairs, related with important traits of rice plant), and 384 SNP markers, and to compare the effectiveness of each technique in distinguishing among genotypes. A field experiment was conducted in Ranca Jaya village, Patok Beusi, Subang, West Java during Wet Season (WS) of 2011/2012, using a Randomized Complete Block Design in three replications. A modified CTAB method was used to extract DNA for detection using 12 SSR markers and 384 SNP markers. The results revealed that the use of SSR markers that were linked to certain genes was more accurate than that of the SNP markers, agronomic, and morphological characters, in distinguishing differences among the 9 rice genotypes. The complete data of morphologic, agronomic, and molecular are useful to distinguish the authenticity of a variety in order to protect the intelectual property right attached on the variety.</p>

scholarly journals DNA Fingerprinting and Genetic Diversity in Aus Rice (Oryza sativa L.) Landraces of Bangladesh

2018 ◽  
Vol 21 (1) ◽  
pp. 59-65
Author(s):  
MA Siddique ◽  
M Khalequzzaman ◽  
K Fatema ◽  
MZ Islam ◽  
MHK Baktiar ◽  
...  
Keyword(s):  
Genetic Distance ◽  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Oryza Sativa L ◽  
Polymorphic Information Content ◽  
Allelic Diversity ◽  
Rice Landraces ◽  
Genetic Groups ◽  
Improvement Programme ◽  
Genetic Coefficient

The allelic diversity and relationships among 48 Aus rice landraces were determined through DNA fingerprinting using microsatellite (SSR) markers. A total of 14 SSR markers for different chromosomes were used to characterize and differentiate the studied rice genotypes. The number of alleles per locus varied from three alleles (RM118) to 18 alleles (RM44) with an average of 9.88. The polymorphic information content (PIC) varied widely among the loci and ranged from 0.3725 (RM107) to 0.9146 (RM519) with an average of 0.7248. The genetic distance-based results found in the UPGMA clustering system revealed six genetic groups with a similarity coefficient of 0.35. Chakila and Shitki saitta had closest distance in the SSR based genetic distance might have same genetic background. Based on genetic coefficient, the diverse landraces Kasalot, Balam, Pankhiraj, Dular, Hashikalmi, Galong, Panbira, Marichbati, Pidi and Surjomoni could be selected as potential parents for varietal improvement programme. The findings of this study should be useful for varietal identification and could be useful for plant breeders in selecting suitable genetically diverse parents for the crossing programmeBangladesh Rice j. 2017, 21(1): 59-65

scholarly journals DNA fingerprinting of foxtail millet (Setaria italica L.) variety ATL 1 using SSR and RAPD markers along with morphological descriptors

2020 ◽  
Vol 7 (3) ◽  
pp. 587-593
Author(s):  
Senthil Natesan ◽  
◽  
Gowtham Murugesan ◽  
Nandhini Murugan ◽  
Sarankumar Chandran ◽  
...  
Keyword(s):  
Dna Fingerprinting ◽  
Ssr Markers ◽  
Rapd Markers ◽  
Plant Genetic Resources ◽  
Foxtail Millet ◽  
Setaria Italica ◽  
National Bureau ◽  
Genetic Purity ◽  
Purity Testing ◽  
Ssr Primers

Foxtail millet (Setaria italica) is a cultivated nutritional cereal, which originated in South Asia and is considered one of the oldest cultivated millets in India. DNA fingerprinting is mandatory for registration of newly developed varieties with National Bureau of Plant Genetic Resources (NBPGR) and Protection of Plant Varieties and Farmers' Rights Authority (PPV&FRA). Due to the limited availability of genomic information in foxtail millet, the use of DNA based markers in fingerprinting of crop varieties is also limited. Hence in the present investigation, available RAPD and SSR markers of cereals are used for fingerprinting the foxtail millet varieties. The newly released variety ATL 1 is differentiated from popular variety CO (Te) 7 using SSR and RAPD markers. About 66 maize SSR primers, 16 sorghum SSR primers, and 10 RAPD primers were used in the study. Out of 66 maize SSR markers used for study, one showed polymorphism. The marker umc1704 showed polymorphism between CO (Te) 7 and ATL 1 by the presence of 670 bp allele CO (Te) 7. The RAPD primers OPB4, OPA5, OPA11 and OPB1 also helped for differentiation of the two varieties. The identified makers will help for genetic purity testing of CO (Te) 7 and ATL 1 in the seed chain.

scholarly journals DNA Finger-Printing: Current Scenario and Future

2021 ◽  
Author(s):  
Sandeep Sitaram Kadu
Keyword(s):  
Dna Fingerprinting ◽  
Double Helix ◽  
Genetic Material ◽  
Bill Clinton ◽  
Human Being ◽  
Nitrogen Base ◽  
Human Myoglobin ◽  
Finger Printing ◽  
Current Scenario ◽  
Dna Finger Printing

Linearly arranged chemical structure in chromosome is known as DNA. It is a double helix made up of two strands of genetic material spiraled around each other. Each strand has a sequence of bases. There are four types of basis namely adenine, guanine, cytosine and thiamine which are very unique to each individual just like their actual fingerprint. The nitrogen base adenine always binds with thymine and cytosine also always binds with guanine. Thus the DNA profiling unique to each individual is collectively known as DNA fingerprinting. DNA determines individuality or uniqueness of the each human being except in uniovular twins. The chances of complete similarity are one in 30 billion to 300 billion i.e. half the population of world. The technique of DNA fingerprinting was first developed by Dr. Alec Jeffery’s from Britain in 1984. He discovered a minisatellite region close to the human myoglobin gene. He isolated this sequence and used it as a probe to investigate human DNA. He found that the minisatellite probe result was a complex band pattern for each individual. In India, initially it was done at CCMB, Hyderabad by Dr. Lalji Singh. Now there are various centers where DNA fingerprinting is carried out. In Maharashtra it is carried out at Sate Forensic Science Laboratory, Vidya Nagar, Kalina, Mumbai – 400 098 (Phone 022–26670755). Using this technique FBI formally concluded the participation of Mr. Bill Clinton in Monica Lewyninskey case. In India more than 79 cases have been solved by using this technique including important case of Dhanu and Shivarasan alleged assailant of Late Priminister Shr. Rajiv Gandhi, Tandori case, Madhumati murder case etc.

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