ĐA DANG DI TRUYỀN CỦA 120 GIỐNG/DÒNG ĐẬU NÀNH (Glycine max (L.) Merr.) BẰNG CHỈ THỊ PHÂN TỬ SSR

Nghiên cứu đa dạng di truyền nhằm mục đích tìm ra mối quan hệ giữa các kiểu gen trong tập đoàn giống/dòng cây trồng, từ đó có thể đưa ra chiến lược chọn tạo giống, cải thiện nguồn gen. Nghiên cứu này đã sử dụng 09 chỉ thị phân tử SSR để đánh giá mức độ đa dạng di truyền của 120 giống/dòng đậu nành (Glycine max (L.) Merr.) đang được bảo tồn tại ngân hàng giống trường Đại học Cần Thơ. Kết quả điện di sản phẩm PCR bằng 09 chỉ thị phân tử SSR thu được 52 phân đoạn và tất cả 52 phân đoạn đều có tỷ lệ đa hình trung bình cao (100%). Chỉ số PIC dao động từ 0,05 (satt596) đến 0,46 (satt009), với giá trị trung bình là 0,21. Cây phả hệ được xây dựng dựa trên 09 chỉ thị SSR bằng phân tích nhóm UPGMA phân các mẫu thành 11 nhóm chính với hệ số di truyền trung bình là 0,7 và hệ số tương đồng dao động từ 0,47 - 0,87. Kết quả này cho thấy bộ sưu tập 120 giống/dòng đậu nành rất đa dạng về bản chất di truyền và có thể dùng làm vật liệu ban đầu cho công tác chọn tạo giống đậu nành trong tương lai. ABSTRACT Genetic diversity research aims to study the relationship between genotypes in the varieties/lines, as the results, a breeding strategy will be set up for genetic improvement. In this study, 09 SSR molecular markers were used to evaluate the genetic diversity of 120 soybean varieties/lines being conserved at the gene bank of Can Tho University. A total of 52 fragments were produced by 09 SSR primers with 100% polymorphism rate. The PIC index value was ranged from 0.05 (satt596) to 0.46 (satt009), the average PIC index was 0.21. Using UPGMA analysis showed that the phylogenetic tree was divided 120 soybean varieties/lines into 11 main groups with the average genetic coefficient of 0.7 and the similarity coefficient ranging from 0.47-0.87. Thus, this result showed that the collection of 120 soybean varieties/lines is very diverse in genetic background and can be used as a starting material for future soybean breeding.

Sensitivity Analysis of CERES-Wheat Model under Hisar Conditions

The field experiment was conducted at Research Farm, Dept of Agricultural Meteorology, CCS HAU, Hisar (Lat.: 290 10’ N, Log.: 75036’ E & 215.2 m above msl), Haryana, India during the rabi season of 2014-15 and 2015-16. Experimental designed laid out with strip plot, as main plot treatment: four growing environments i.e. D1: 5th Nov., D2: 20th Nov., D3: 5th Dec. and D4: 20th Dec. and sub-plot treatment: four irrigation regimes, it applied at different phenophases (I1-CRI, I2- CRI and heading, I3- CRI+ jointing and milking, I4- CRI+ jointing + anthesis and dough stage). Crop growth and yield data of 2014-15 were used for calibration of DSSAT model and cultivar coefficients for WH1105 based on the observed crop characteristics. Genetic coefficient parameters are in the ranges obtained by the few other studies conducted on wheat with the exception of parameters G1, G2, and G3, related to grain growth. Sensitivity of simulated grain yield to down scaled sunshine hours, solar radiation -0.5 to -2.5 hours and -1°C to -5 MJ-2 day-1 showed a gradual decrease in grain yield, respectively.

Parameter genetik kultivar-kultivar jagung local pada cekaman salinitas sedang

Produktivitas jagung di Sulawesi Tengah masih relatif rendah dibanding produksi nasional sehingga perlu ditingkatkan melalui pemuliaan tanaman. Penelitian bertujuan untuk mengkaji parameter genetik tanaman jagung pada cekaman salinitas sedang. Penelitian dilaksanakan pada Juni sampai Agustus 2019, di Green House Fakultas Pertanian, Universitas Tadulako, Palu. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) terdiri atas 6 perlakuan genotip dan diulang 3 kali serta 5 unit tanaman per perlakuan sehingga terdapat 90 unit percobaan. Parameter genetik yang di analisis adalah koefisiean keragaman genotipik, koefisien keragaman fenotipik, heritabilitas, kemajuan genetik, korelasi, dan analisis sidik lintas. Hasil penelitian menunjukkan bahwa kehijauan daun, bobot tongkol berkelobot, berat tongkol tidak berkelobot, dan berat biji pertongkol memiliki koefisien keragaman genetik tinggi. Kehijauan daun, berat tongkol berkelobot, bobot tongkol tidak berkelobot, panjang tongkol tidak berkelobot, diamater tongkol, berat biji per tongkol dan bobot 100 biji memiliki nilai heritabilitas dan kemajuan genetik tinggi. Seleksi secara tidak langsung dapat dilakukan pada umur panen agar diperoleh hasil jagung lokal yang tinggi pada kondisi tercekam salinitas sedang. Karakter-karakter tersebut dapat dijadikan acuan dalam menyeleksi tanaman jagung dengan cekaman salinitas sedang untuk program pemuliaan jagung.AbstractThe productivity of maize in Central Sulawesi is relatively low compared to national production and needs to be improved by plant breeding. The study aimed to examine the genetic parameters of the maize plant traits at moderate salinity stress. The research was conducted from June to August 2019, at the Green House of the Faculty of Agriculture, Tadulako University, Palu. The genetic parameters analyzed were genetic coefficient of variation, phenotypic coefficient of variation, heritability, genetic advance, correlation, and path analysis. The experimental design used a completely randomized design consisting of six genotypic treatments and repeated three times. The results showed that the leaves greenness, the weight of the cob with and without husk, and weight of seeds per cob had a high genetic coefficient of variation. Leaf greenness, the weight of the ear with and without husk, ear length without husk, ear diameter, seed weight per ear and yield have a high value of heritability and genetic advance. Indirect selection can be applied through harvest time trait to obtain a high local maize yield in moderate salinity stress condition. These traits can be used as a reference in selecting maize plants with moderate salinity stress for maize breeding programs.

Evaluation of Wild Segments Related to Growth Period Traits Using a Modified Wild Soybean (Glycine Soja Sieb. Et Zucc.) Chromosome Segment Substitution Line Population

Soybean seeds contain high levels of oil and protein providing 69% and 57% of dietary protein and oil respectively. Although many quantitative trait loci for 100-seed weight (100SW), protein content (PRC) and oil content (OIC) have been reported, their genetic controls in soybeans remain unclear. The QTL–allele constitution of three traits in the Sichuan and Chongqing eco-regions population (SCLBP) was studied using a representative sample composed of 228 accessions tested under four environments and analyzed based on 135 SSR and 107081 valid SNP (single nucleotide polymorphism linkage) markers. The varied range of 100SW, PRC and OIC of SCLBP were 4.82-33.35, 36.47-49.75 and 14.68-21.77 among accessions, respectively. The heritability (h2) and genetic coefficient of variation (GCV) of three traits were high. As a result, 26, 33 and 31 QTLs were found by SSR to be associated with 100SW, PRC and OIC, respectively. The allele of Sat_260 for 100SW was detected in 4 environments. In addition, 28, 198 and 250 loci for 100SW, PRC and OIC were found by SNP using mixed linear model (MLM), respectively. Further SNP haplotype analysis showed that 13, 35 and 60 blocks for 100SW, RPC and OIC were found. The block of Gm11_9895764-9917646 for 100SW was simultaneously detected in four environments. Among these QTLs, 1, 5, and 7 for 100SW, PRC and OIC were found by two methods of SSR and SNP at the same time. A majority of these QTLs overlapped with the previously reported loci. However, 9, 11 and 9 loci for 100SW, PRC and OIC using SSR and 3, 5 and 8 for 100SW, PRC and OIC haven’t been reported using SNP in the study, respectively. Moreover, the genes of Glyma.11g130800, Glyma.13g217000 and Glyma.08g122600 were considered to be the most likely genes controlling 100SW, PRC and OIC, respectively. Our findings provide evidence for mixed major plus polygenes inheritance for three traits and an extended understanding of their genetic architecture for molecular dissection and breeding utilization in soybeans.

Quantitative Evaluation of Plant Priority Protection in Cao Lake Wetland Nature Reserve

Based on the survey data of plant resources in Cao Lake Wetland Nature Reserve, using analytic hierarchy process (AHP) and square root method, the evaluation system of plant priority protection was constructed. From three aspects of endangered coefficient, genetic coefficient and species coefficient, the plant species in the reserve were evaluated quantitatively and comprehensively, and the protection levels were divided. The results showed that there were 179 plant species in nature reserve, including 5 endangered species, 9 vulnerable species, 41 near threatened species and 124 least concerned species. The priority protection levels of plants were determined, including 1 species of the primary protection, 12 species of the secondary protection, 20 species of the tertiary protection and 146 species of the delayed protection, and corresponding protection suggestions were put forward. The purpose is to provide more effective background information and data support for the plant protection work and to help protect biodiversity.

Genetic Variation for Striga hermonthica Resistance and Yield Among Sorghum Accessions in Nigeria

Striga hermonthica (Delile) Benth., commonly referred to as witch weed, is a major constraint to sorghum (Sorghum bicolor (L.) Moench) production in the Northern region of Nigeria because of high yield losses due to infestation. To identify parental lines useful in breeding for S. hermonthica resistant sorghum genotypes adapted to Nigeria, twenty-five sorghum accessions were evaluated in Nigeria across three test environments. Both phenotypic and genetic components influenced the variation observed in the sorghum accessions. The estimates for the genetic coefficient of variation, heritability and genetic advance for the area under Striga number progress curve (ASUNPC), Striga emergence counts, yield and other agronomic traits, obtained in this study revealed that genetic gain for resistance to S. hermonthica could be realized through selection. Based on the performance of the 25 sorghum accessions SRN39, Danyana, Sepon82, and SAMSORG40 were the top four accessions found to be most resistant to S. hermonthica. Assessment of resistance was based on the low Striga emergence counts and the ASUNPC values. These accessions can be used as donor sources of S. hermonthica resistant genes for introgression into cultivars adapted to Nigeria, followed by recombination breeding for pyramiding the different resistance mechanisms.

Estimation of genetic parameters for micro-environmental sensitivities of production traits in Holstein cows using two-step method

Context The request for more uniform animal products, which is motivated chiefly by economic reasons, has enhanced the interest in decreasing variability of characters via selection. In the present dairy operation, breeding dairy cows which have strong resistance against environmental changes for main traits is very important. Aims The aim of this study was to estimate genetic parameters for heterogeneity of residual variance in milk yield and composition of Iranian Holstein cows. Methods The dataset included 305-day production records of cows which were provided by the Animal Breeding Center and Promotion of Animal Products of Iran between 1983 and 2014. In two-step method, univariate analyses were conducted to estimate variance components for 305-day production traits. Then, genetic variability of residual variances was estimated. Key results Estimates of heritability for micro-environmental sensitivities of milk, fat and protein yields in the first three lactations of Holstein cows were low and equal to 0.043, 0.028 and 0.039; 0.031, 0.019 and 0.024; 0.027, 0.016 and 0.019 respectively. Considerable genetic coefficient of variations of residual variance for above mentioned traits (0.261, 0.247 and 0.218; 0.221, 0.204 and 0.194; 0.219, 0.199 and 0.178 respectively) indicated significant additive genetic variation for micro-environmental sensitivities. Conclusions The results of this study indicate that micro-environmental sensitivities were present for milk production traits of Iranian Holsteins. High genetic coefficient of variation for micro-environmental sensitivities indicated the possibility of reducing environmental variation and increase in uniformity via selection. Implications Reduction of environmental sensitivities would increase the predicted performance of animals and decreased corresponding threats for dairy farmers.

Quantitative Evaluation of Plant Priority Protection in Liangzi Lake Wetland Nature Reserve

Based on the field survey data and relevant literatures, the evaluation index system of plant priority protection, which consists of threatened coefficient, genetic coefficient and species coefficient was established. Quantitative study and comprehensive evaluation of plant resources in Liangzi Lake Nature Reserve were carried out, and protection levels were classified. The results showed that there were 254 plant species in nature reserve, including 18 endangered species, 46 vulnerable species, 76 lower risk species and 114 safety species. The coefficients of threatened, genetic and species were analyzed and calculated by the indexes and proportion weights. The priority protection order of plants was determined, including 12 species of the first class conservation, 18 species of the second class conservation, 33 species of the third class conservation and 191 species of the delayed conservation. The corresponding protection suggestions were put forward. The purpose is to provide theoretical guidance for plant protection work and to help protect biodiversity of Liangzi Lake Nature Reserve.

DNA Fingerprinting and Genetic Diversity in Aus Rice (Oryza sativa L.) Landraces of Bangladesh

The allelic diversity and relationships among 48 Aus rice landraces were determined through DNA fingerprinting using microsatellite (SSR) markers. A total of 14 SSR markers for different chromosomes were used to characterize and differentiate the studied rice genotypes. The number of alleles per locus varied from three alleles (RM118) to 18 alleles (RM44) with an average of 9.88. The polymorphic information content (PIC) varied widely among the loci and ranged from 0.3725 (RM107) to 0.9146 (RM519) with an average of 0.7248. The genetic distance-based results found in the UPGMA clustering system revealed six genetic groups with a similarity coefficient of 0.35. Chakila and Shitki saitta had closest distance in the SSR based genetic distance might have same genetic background. Based on genetic coefficient, the diverse landraces Kasalot, Balam, Pankhiraj, Dular, Hashikalmi, Galong, Panbira, Marichbati, Pidi and Surjomoni could be selected as potential parents for varietal improvement programme. The findings of this study should be useful for varietal identification and could be useful for plant breeders in selecting suitable genetically diverse parents for the crossing programmeBangladesh Rice j. 2017, 21(1): 59-65