scholarly journals Distinguishing Rice Genotypes using Morphological, Agronomical,

2015 ◽  
Vol 34 (2) ◽  
pp. 79 ◽  
Author(s):  
Untung Susanto ◽  
Nofi Anisatun Rohmah ◽  
Made Jana Mejaya
Keyword(s):  
Ssr Markers ◽  
Block Design ◽  
Rice Variety ◽  
Morphological Characters ◽  
Snp Markers ◽  
Complete Data ◽  
Property Right ◽  
Wet Season ◽  
Rice Varieties ◽  
Rice Genotypes

<p align="LEFT">Complete data on characteristics of a rice variety is very important to  race the authenticity of the variety at the field. Sometimes a name of a variety had changed, due to the informal  seed distribution among farmers. This could become problem in the property right of the variety. Distinguishing among rice varieties using only morphological and agronomical traits are sometimes not sufficient. Currently, molecular markers such as SSR (Simple Sequence Repeats) and SNP (Single Nucleotide Polymorphism) markers have become available and are powerfull to distinguish rice genotypes. This research was aimed to distinguish nine rice varieties grown by farmers, using morphological characters (47 traits), agronomical characters (9 traits), SSR markers (12 primer pairs, related with important traits of rice plant), and 384 SNP markers, and to compare the effectiveness of each technique in distinguishing among genotypes. A field experiment was conducted in Ranca Jaya village, Patok Beusi, Subang, West Java during Wet Season (WS) of 2011/2012, using a Randomized Complete Block Design in three replications. A modified CTAB method was used to extract DNA for detection using 12 SSR markers and 384 SNP markers. The results revealed that the use of SSR markers that were linked to certain genes was more accurate than that of the SNP markers, agronomic, and morphological characters, in distinguishing differences among the 9 rice genotypes. The complete data of morphologic, agronomic, and molecular are useful to distinguish the authenticity of a variety in order to protect the intelectual property right attached on the variety.</p>

scholarly journals Genetic Diversity Analysis of 53 Indonesian Rice Genotypes using 6K Single Nucleotide Polymorphism Markers

2018 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Joko Prasetiyono ◽  
Nurul Hidayatun ◽  
Tasliah Tasliah
Keyword(s):  
Genetic Diversity ◽  
Morphological Characters ◽  
Snp Markers ◽  
Japonica Rice ◽  
Rice Varieties ◽  
Tropical Japonica ◽  
Rice Chromosomes ◽  
Improvement Programs ◽  
Rice Genotypes ◽  
Genotypic Mixtures

<p>Indonesia is rich in rice genetic resources, however, only a small number has been used in variety improvement programs. This study aimed to determine the genetic diversity of Indonesian rice varieties using 6K SNP markers. The study was conducted at ICABIOGRAD for DNA isolation and IRRI for SNP marker analysis. Genetic materials were 53 rice genotypes consisting of 49 varieties and 4 check genotypes. SNP markers used were 6K loci. Results showed that among the markers analyzed, only 4,606 SNPs (76.77%) were successfully read. The SNP markers covered all twelve rice chromosomes of 945,178.27 bp. The most common allele observed was GG, whereas the least allele was TG. Dendrograms of the 53 rice varieties analyzed with 4,606 SNPs demonstrated several small groups containing genotypic mixtures between indica and japonica rice, and no groups were found to contain firmly indica or japonica type. Structure analysis (K = 2) with value of 0.8 showed that the 53 rice varieties were divided into several groups and each group consisted of 4 japonica, 2 tropical japonica, 46 indica, and 1 aus rice type, respectively. IR64 and Ciherang proved to have an indica genome, while Rojolele has japonica one. Dupa and Hawara Bunar, usually grouped into tropical japonica rice, were classified as indica type, and Hawara Bunar has perfectly 100% indica type. The results of this study indicated that rice classification (indica-japonica) which is usually classified based only on morphological characters, e.g. grain and leaf shapes, is not enough and classification based on SNP markers should be considered for that purpose.</p>

scholarly journals Performance of hybrid Boro rice in coastal area of Bangladesh

2019 ◽  
Vol 30 (2) ◽  
pp. 186-193
Author(s):  
R Mahmood ◽  
MHK Howlader ◽  
MZ Haque
Keyword(s):  
Growth Rate ◽  
Leaf Area ◽  
Coastal Area ◽  
Block Design ◽  
Morphological Characters ◽  
Research Field ◽  
Rice Varieties ◽  
Area Index ◽  
Rice Genotypes ◽  
Boro Rice

The present experiment was conducted at the research field of Patuakhali Science and Technology University (PSTU), Patuakhali to evaluate the Performance of hybrid boro rice (genotypes) in coastal area of Bangladesh. The experiment consisted of five rice varieties as treatment such as Arize Tej, Tea Sakti, Shathi and BRRI Dhan 28. Data were collected on morphological characters such as plant height, number of leaves plant-1 and leaf area hill-1, growth characters such as leaf area index (LAI), crop growth rate (CGR) and relative growth rate (RGR), yield and yield components such as number of effective tillers hill-1,number of non effective tillers hill-1, panicle length, number of filled grains panicle-1, number of unfilled grains panicle-1, 1000 grain weight, grain yield, straw yield, biological yield and harvest index (%) were recorded. The experiment was laid out in a completely randomized block design (RCBD) with three replications. The collected data were analyzed statistically and means were adjudged by DMRT at 5% level of probability. Among the five varieties the Arize Tej gave the highest performance. From the above investigated results, it was observed that the Arize Tej was the most efficient for better growth and higher yield of  hybrid boro rice genotypes grown in coastal area of Bangladesh. Progressive Agriculture 30 (2): 186-193, 2019

scholarly journals Evaluation of Short Duration Drought Tolerant Rice Varieties in Drought Prone Areas of Subarnapur District of Odisha

2020 ◽  
pp. 21-26
Author(s):  
S. Pradhan ◽  
F. H. Rahman ◽  
S. Sethy ◽  
G. Pradhan ◽  
J. Sen
Keyword(s):  
Short Duration ◽  
Upland Rice ◽  
Plant Protection ◽  
Block Design ◽  
Rice Variety ◽  
Rice Varieties ◽  
Agronomic Practices ◽  
Drought Tolerant ◽  
Randomized Block Design ◽  
Sowing Seed

A study was conducted in farmer’s field through technology demonstrations during Kharif season of 2018 and 2019 in Subarnapur district of Odisha to recommend the most suited short duration drought tolerant upland rice variety particular to the locality. Six different varieties like Sahabhagi dhan, Mandakini, Jogesh, DRR-44, and DRR-42 & Khandagiri were taken with four replications in Randomized Block Design in both years. Technology demonstration included not only the improved short duration varieties, but also a package of good agronomic practices viz. line sowing, seed treatment with Bavistin, proper fertilization with dose @ 80:40:40 NPK/ha, pre emergence herbicide application with pretilachlor, needful manual weeding and  need based plant protection chemicals. The results of demonstration showed that farmers could increase rice productivity notably by adopting improved variety Sahabhagi dhan and DRR-44. These varieties recorded higher grain yields of 64% and 46%, respectively, and fetched a sum return of Rs 25620 and Rs 25275 over farmers’ local variety Khandagiri.

scholarly journals Selection of drought tolerant rice varieties for the western mid hills of Nepal

2018 ◽  
pp. 195-206
Author(s):  
B. B. Adhikari ◽  
B. Mehera ◽  
S. M. Haefele
Keyword(s):  
Oryza Sativa L ◽  
Block Design ◽  
Cropping System ◽  
Root Weight ◽  
Total Production ◽  
Wet Season ◽  
Growth Duration ◽  
Rice Varieties ◽  
Participatory Varietal Selection ◽  
Drought Tolerant

Rice (Oryza sativa L) is the most important staple food crop in Nepal, but current yields are low and total production varies, mostly due to drought. To evaluate high yielding and drought tolerant rice genotypes in typical rainfed lowland conditions, a participatory varietal selection trials was conducted with farmers in farmer’s field at Sundarbazar, Lamjung, Nepal during the wet season 2009 and 2010. Trials were laid out in a Randomized Complete Block Design with six replications. Results obtained from two years’ experiment indicated that the genotypes IR-74371-54-1 and IR74371-70-1 matured at the same duration as the local check Radha-4 (120-123 days growth duration). Both genotypes had a high average chlorophyll content (SPAD reading of 15.9-16.1), were medium tall (110-112 cm), non-lodging, had a high number of filled grains and little sterility, and a high grain yield (mean yield 5.0 and 4.8 t ha-1, respectively). They also had the highest root length (22.4-26.2 cm) and root weight (9.5-10 g hill-1) which could tolerate more drought than the other genotypes tested. The maximum cost/benefit ratio was found in IR-74371-54-1 (1:1.72) followed by IR-74371-70-1 (1:1.66) and IR-74371-46-1-1 (1:1.65). All three lines were highly preferred by farmers in preference rankings during field visits and were released in Nepal in 2011 as Sookhadhan-2, Sookhadhan-3 and Sookhadhan-1 varieties, respectively. They fit easily into the existing cropping system in rainfed lowlands, are economically viable and safe to cultivate for the farmers in the mid hills of Nepal.Journal of the Institute of Agriculture and Animal Science.Vol. 33-34, 2015, page: 195-206

scholarly journals Rajdeep (IET 17713): A New Rainfed Lowland High Yielding Rice Variety for Semideep Situation

2021 ◽  
Author(s):  
Indrani Dana ◽  
Sitesh Chatterjee
Keyword(s):  
West Bengal ◽  
Insect Pests ◽  
Oryza Sativa L ◽  
Rice Variety ◽  
National Level ◽  
Stem Borer ◽  
Research Station ◽  
Wet Season ◽  
Rice Varieties ◽  
Improvement Project

In eastern India, more than 13.0 million ha of rice lands are affected by excess water and periodically suffer from flash floods and complete submergence. Most of the traditional and adapted rice varieties of this situation are low yielders. To overcome these problems the high yielding rice variety (HYV), Rajdeep (IET 17713) has been developed by Rice Research Station, Government of West Bengal, Chinsurah, Hooghly as high yielding rice variety for semideep water situation. Field experiment was conducted to evaluate the performance of promising semideep water rice (Oryza sativa L.) genotypes under lowland situation during wet season of 2002-2013. Performance of Rajdeep, semideep water rice was better and therefore it was released. It is tolerant to sheath blight, sheath rot diseases and stem borer, leaf folder insect-pests. The high yielding semideep rice variety, Rajdeep exhibited superiority over national check (Sabita), regional check (Purnendu) and local check tested in different locations under All India Coordinated Rice Improvement Project (AICRIP) trials during kharif, 2002-2003 conducted by ICAR-Indian Institute of Rice Research, Hyderabed, India. This variety was also tested in multi-locational yield trials (2003-2013) along with check variety Swarna-Sub 1/ Bhudeb at different locations of West Bengal. After testing at national level as well as at state level, based on yield performance of the variety, the HYV semideep rice variety Rajdeep was released and notified by Government of India in the Gazette of India vide Notification No. S.O. 1007(E) on 30th March, 2017.

scholarly journals RESPONSE OF SUB1 INTROGRESSION LINES OF RICE TO VARIOUS FLOODING CONDITIONS

2013 ◽  
Vol 14 (1) ◽  
pp. 15 ◽  
Author(s):  
Yudhistira Nugraha ◽  
Georgiana V. Vergara ◽  
David J. Mackill ◽  
Abdel Bagi Ismail
Keyword(s):  
Block Design ◽  
Soluble Sugar ◽  
Chlorophyll Concentration ◽  
Flash Floods ◽  
Introgression Lines ◽  
Yield Potential ◽  
Rice Varieties ◽  
Panicle Number ◽  
Rice Genotypes ◽  
Soluble Sugar And Starch

Two types of floods can be happen in rice crops, i.e. flash floods and  stagnant floods. Flash floods cause complete submergence for up to 2 weeks, while stagnant floods (SF) could partially submerge part of rice  plant. To overcome yield loss due to the floods, introgression of SUB1 gene, known as a gene suppressing cell elongation and carbohydrate  metabolism, to rice genotype can increase plant tolerance to complete submergence for 10 days or more. The study aimed to evaluate the response of 18 rice genotypes, including the recently developed sixth pair SUB1 near isogenic lines (NILs) of mega-rice varieties (Swarna, Sambha Mahsuri, IR64, TDK1, BR11, and CR1009), to various flooding conditions. The rice genotypes were planted at field ponds at Los Banos, Philippines, in the wet season (WS) of 2009. The treatments were 15 days  submergence, SF, SF follows submergence and normal conditions. Each treatment was arranged in completely randomized block design with three<br />replications. The results showed that the SUB1 introgression rice lines had higher survival compared to the non-SUB1 and did not much elongate their shoots during submergence. Nevertheless, under SF the rice genotypes should elongates their shoots to allow restoring contact with the air. SF and SF follows submergence decreased the panicle number, grain<br />number per panicle and panicle fertility. Consequently, the yield declined. It suggests that sensitive genotypes are mostly sourcelimited during grain filling. The SUB1 introgression lines had higher chlorophyll concentration and less depletion in soluble sugar and starch after submergence. Under SF, soluble sugar and starch contents between the SUB1 NILs and  non-SUB1 lines were not significantly different. Introgression of the SUB1 into high-yielding varieties improved submergence tolerance without affecting yield potential. The study indicates that introgression of the SUB1 into taller type rice varieties should be done to compensate the effect of suppressed elongation. <br />

scholarly journals Pengaruh Pupuk Kandang dan Kesesuaian Varietas-Musim Tanam terhadap Hasil Padi Sawah

2014 ◽  
Vol 33 (3) ◽  
pp. 177
Author(s):  
Gagad Restu Pratiwi ◽  
Sumarno Sumarno
Keyword(s):  
Grain Yield ◽  
Organic Fertilizer ◽  
Rice Variety ◽  
Brown Planthopper ◽  
Production Capacity ◽  
Residual Effect ◽  
Wet Season ◽  
Rice Varieties ◽  
Significant Interaction Effect ◽  
Manure Treatment

Adaptive variety and organic fertilizer are important components for rice cultural practices in a spesific agroecology. The research objectives were (1) to clarify the direct and the residual effect of manure on grain yield of rice, (2) to identify rice varieties most suitable for dry and for wet season planting. The research was conducted at Sukamandi Exp. Farm, West Java, during dry season (DS) of 2010 and wet season (WS) of 2010/2011, using a split-plot design with three replications. The main plots of DS experiment consisted of (1) recommended dosage of NPK (115 kg N + 50 kg P2O5 + 50 kg K2O/ha) and (2) 80% dosage of NPK + 5 t manure/ha. The WS experiment, main plots were (1) recommended NPK and (2) the residue of manure given in DS + 80% of the NPK. The subplots were fifteen improved varieties, each planted on plot 3.4 m x 5.4 m with 20 cm x 20 cm plant spacing. Data were collected for agronomic variables and grain yield. The DS 2010 experiment was attacked by brown planthopper (BPH), only two varieties, Inpari-8 and Inpari-13 were resistant, each yielded around 6 t/ha, while thirteen varieties were susceptible, yielded between 0.14 t to 3.9 t/ha. Application of 5 t manure/ha increased the averaged of biomass and grain yields by 9% and 10%, respectively, but were not consistent among varieties. In WS 2010/2011 experiment, the residual manure + 80% NPK decreased the averaged grain yield, but there was a significant interaction effect between fertilizer x variety. Seven varieties yielded less, six varieties yielded similar, and two varieties yielded more on the residual manure treatment, compared to that on no-manure treatment. Biomass weight, plant height, days to flowering, and days to harvesting, each was significantly correlated with grain yield. Five varieties were identified as suitable for WS planting, namely: Inpari-3, Inpari-10, Inpari-7, Ciherang, and Mekongga. When outbreak of BPH was suspected in the DS, Inpari-8 and Inpari-13 were suggested for planting. Suitable variety(ies) for season specific planting would optimize productivity, conversely, planting of unsuitable rice variety, during either DS or WS planting, would decreased the land production capacity.

scholarly journals Similarity of 26 New Released Rice Varieties and Rice Parental Hybrids Based on 36 SSR Markers

2014 ◽  
Vol 33 (2) ◽  
pp. 71
Author(s):  
Untung Susanto ◽  
Satoto Satoto ◽  
Nofi A. Rohmah ◽  
Made Jana Mejaya
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Ssr Markers ◽  
Rice Varieties ◽  
Average Value ◽  
Modified Method ◽  
Rice Chromosomes ◽  
Medium Level ◽  
Rice Genotypes ◽  
Parental Lines

More than 200 rice varieties had been released in Indonesia, but the genetic variability among those released varieties was suspected to be relatively low. Molecular markers, especially SSR could be used as a tool to disect the distinctness among rice genotypes, albeit phenotipically similar varieties. The technique could also be used to prove the authenticity of a variety. This research was aimed to obtain DNA fingerprinting data of new released rice varieties and hybrid parental lines using SSR markers. A total of 26 rice genotypes consisted of three upland, ten irrigated, five swampy rice varieties, along with eight hybrid parental lines were used in this experiments. The DNA was extracted from young leaf samples using CTAB modified method and was amplified with 36 SSR markers linked to important rice traits which spread accross the 12 rice chromosomes. The experiment was conducted in Plant Breeding Laboratory of Indonesian Center for Rice Research (ICRR) during 2012. The results showed that PIC value of the genotypes were mostly at medium level of the genetic diversity with the average value of 0.4451. The phylogenetic analysis showed that at the genetic distance of 10%, the genotypes were separated into 9 groups, i.e. Inpago 6, Inpara 5, and BH33d each stood alone while (Inpara 1, Inpara 2 , and Inpara 3); (Inpari 18 and Inpari 19, Inpago 4, Inpago 5, and Inpara 4); (Inpari 11, Inpari 12, Inpari 13, Inpari 14, Inpari 15, Inpari 16, Inpari 17, and Inpari 20); (GMJ6B, B6, and IR79156B); (PK21, BH95E, Bio9, and R14) each belong to one group. The grouping of the genotypes in this study seemed to follow the adaptation type to agro ecosystems. The hybrid parental lines tended to stay in different group from the inbred varieties. The application of these 36 SSR markers was able to distinguish among 26 genotypes rather distinctly.The use of more markers should give more powerful data to distinguish among genotypes.

scholarly journals Development of Drought Tolerant Rice Variety BRRI dhan66 for Rainfed Lowland Ecosystem of Bangladesh

2020 ◽  
Vol 23 (1) ◽  
pp. 45-55
Author(s):  
M A Kader ◽  
T L Aditya ◽  
R R Majumder ◽  
T K Hore ◽  
A K M Shalahuddin ◽  
...  
Keyword(s):  
Flag Leaf ◽  
Rice Variety ◽  
Economic Loss ◽  
Reproductive Stage ◽  
Wet Season ◽  
Rice Varieties ◽  
Commercial Cultivation ◽  
Drought Tolerant ◽  
Rainfed Lowland ◽  
Prone Area

A newly released drought tolerant rice variety BRRI dhan66 suitable for rainfed lowland ecosystem of Bangladesh is an improvement over existing drought tolerant rice varieties. The variety has satisfactorily been passed in the proposed variety trial conducted in the farmers’ field. As a result, National Seed Board (NSB) approved this variety for commercial cultivation in the wet season (T. Aman) in 2014. It has modern plant type with 120 cm plant height and matures in 110-115 days. The important feature of this variety is higher drought tolerance during reproductive stage. It can produce 4.5-5.0 t/ha grain yield without irrigation during reproductive stage. It can produce satisfactory yield when soil moisture remains <20% and perch water table depth is more than 70-80 cm from the surface. The seed size of the variety is 24 g with intermediate long bold grain. It has long, wide and erect flag leaf with deep green colour. It is highly promising as a drought tolerant rice variety for cultivation in the drought prone area, which helps farmers’ to get rid of huge economic loss and is contributing in sustaining food security. Bangladesh Rice j. 2019, 23(1): 45-55

Evaluation of the tolerance of Thai indigenous upland rice germplasm to early drought stress using multiple selection criteria

2015 ◽  
Vol 15 (2) ◽  
pp. 109-118 ◽  
Author(s):  
Kittichai Narenoot ◽  
Tidarat Monkham ◽  
Sompong Chankaew ◽  
Patcharin Songsri ◽  
Wattana Pattanagul ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Selection Criteria ◽  
Upland Rice ◽  
Block Design ◽  
Rice Variety ◽  
Tolerance Index ◽  
Rice Varieties ◽  
Rice Germplasm ◽  
Drought Tolerance Index

Drought remains the most important factor that affects rice productivity, especially in rainfed areas, worldwide. Upland rice is one of the crop choices of farmers in the rainfed environment. Although upland rice varieties require less water than lowland rice varieties, yields often remain limited by drought, particularly in the period of early growth. The aims of this study were to identify the traits related to early drought tolerance in upland rice varieties, and to identify the potential sources of germplasm for early drought tolerance. A total of sixty upland rice varieties were planted in a factorial experiment with a randomized complete block design with 3 replications in the rainy seasons of 2011 and 2012, under greenhouse conditions. Based on the drought tolerance index (DTI), the test germplasm sources were classified into three groups: (i) susceptible; (ii) moderately tolerant; (iii) tolerant to drought stress. Grain yield (GY) showed significant negative correlations with the leaf rolling score (r= − 0.623, P< 0.01), the leaf death score (LDS) (r= − 0.673, P< 0.01) and the recovery score (r= − 0.746, P< 0.01), while leaf dry matter (r= 0.698, P< 0.01) and leaf water potential (r= 0.618, P< 0.01) had significant positive correlations with GY. These findings indicate the suitability of the DTI as the selection criteria for early drought tolerance in a breeding programme. In addition, the upland rice germplasm accessions KKU-ULR011, KKU-ULR012, KKU-ULR125, KKU-ULR199 and KKU-ULR292 were identified as having high levels of stability for drought tolerance in both the 2011 and 2012 experiments, suggesting their potential for further use for rice variety improvement for drought tolerance.

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