scholarly journals Comparative analysis of bHLH transcription factors in five Rosaceae species, and expression analysis of PbbHLHs in response to drought stress in pear

2020 ◽  
Author(s):  
Xiaobing Kou ◽  
Changlong Xiong ◽  
Danqi Wang ◽  
Yangyang Sun ◽  
Peng Wang ◽  
...  
Keyword(s):  
Drought Stress ◽  
Gene Family ◽  
Expression Analysis ◽  
Tissue Expression ◽  
Evolutionary Pattern ◽  
Helix Loop Helix ◽  
Tissue Expression Analysis ◽  
Fruit Species ◽  
Rosaceae Species ◽  
Duplication Events

Abstract Background The bHLH (basic helix-loop-helix) transcription factor family plays important roles in regulating plant growth and development. However, informations about bHLH in rosaceous fruit species are still limited. Results In this study, a total of 198 PbbHLH genes were identified in the pear, with 188 bHLH genes in apple, 129 bHLH genes in peach, 112 in strawberry and 122 in Chinese plum. These Rosaceae bHLH genes, plus the 150 Arabidopsis thaliana bHLH genes, were divided into 34 groups, which included one Rosaceae-specific group. Evolutionary pattern analysis showed that whole-genome duplication (WGD) and segmental duplication played critical roles in expansion of the PbbHLH gene family. Ks value indicated that the two WGD duplication events (a recent WGD and an ancient WGD event) lead to the expansion of bHLH gene family. Tissue expression analysis shows that PbbHLHs may have diversity functions in different tissues. Furthermore, eight up-regulated and seven down-regulated PbbHLH genes were identified as the candidate genes in response to drought stress. Conclusion A comprehensive analysis of bHLH genes was performed in five Rosaceae species. The phylogenetic, evolution and expression analyses of the bHLH gene family in pear will be meaningful for investigating the biological roles of PbbHLH genes.

scholarly journals Identification and Expression Analysis of Stress-Associated Proteins (SAPs) Containing A20/AN1 Zinc Finger in Cucumber

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 400
Author(s):  
Wei Lai ◽  
Yong Zhou ◽  
Rao Pan ◽  
Liting Liao ◽  
Juncheng He ◽  
...  
Keyword(s):  
Gene Family ◽  
Zinc Finger ◽  
Expression Analysis ◽  
Stress Responses ◽  
Tissue Expression ◽  
Future Research ◽  
Transcriptional Responses ◽  
Cucumis Sativus L ◽  
Tissue Expression Analysis ◽  
Associated Proteins

Stress-associated proteins (SAPs) are a class of zinc finger proteins that confer tolerance to a variety of abiotic and biotic stresses in diverse plant species. However, in cucumber (Cucumis sativus L.), very little is known about the roles of SAP gene family members in regulating plant growth, development, and stress responses. In this study, a total of 12 SAP genes (named as CsSAP1-CsSAP12) were identified in the cucumber genome, which were unevenly distributed on six chromosomes. Gene duplication analysis detected one tandem duplication and two segmental duplication events. Phylogenetic analysis of SAP proteins from cucumber and other plants suggested that they could be divided into seven groups (sub-families), and proteins in the same group generally had the same arrangement of AN1 (ZnF-AN1) and A20 (ZnF-A20) domains. Most of the CsSAP genes were intronless and harbored a number of stress- and hormone-responsive cis-elements in their promoter regions. Tissue expression analysis showed that the CsSAP genes had a broad spectrum of expression in different tissues, and some of them displayed remarkable alteration in expression during fruit development. RT-qPCR results indicated that all the selected CsSAP genes displayed transcriptional responses to cold, drought, and salt stresses. These results enable the first comprehensive description of the SAP gene family in cucumber and lay a solid foundation for future research on the biological functions of CsSAP genes.

Molecular cloning, sequence characteristics, and tissue expression analysis of ECE1 gene in Tibetan pig

Gene ◽  
2015 ◽  
Vol 571 (2) ◽  
pp. 237-244 ◽  
Author(s):  
Yan-dong Wang ◽  
Jian Zhang ◽  
Chuan-hao Li ◽  
Hai-peng Xu ◽  
Wei Chen ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Expression Analysis ◽  
Tissue Expression ◽  
Tibetan Pig ◽  
Tissue Expression Analysis ◽  
Sequence Characteristics

scholarly journals Identification and expression analysis of WRKY gene family under drought stress in peanut (Arachis hypogaea L.)

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0231396 ◽  
Author(s):  
Nannan Zhao ◽  
Meijing He ◽  
Li Li ◽  
Shunli Cui ◽  
Mingyu Hou ◽  
...  
Keyword(s):  
Drought Stress ◽  
Gene Family ◽  
Arachis Hypogaea ◽  
Expression Analysis ◽  
Wrky Gene ◽  
Arachis Hypogaea L

Tissue expression analysis, cloning and characterization of the 5′-regulatory region of the bovine FABP3 gene

2016 ◽  
Vol 43 (9) ◽  
pp. 991-998 ◽  
Author(s):  
Anning Li ◽  
Lijuan Wu ◽  
Xiaoyu Wang ◽  
Yaping Xin ◽  
Linsen Zan
Keyword(s):  
Expression Analysis ◽  
Regulatory Region ◽  
Tissue Expression ◽  
Tissue Expression Analysis

scholarly journals Genome-Wide Identification and Functional Exploration of SBP-Box Gene Family in Black Pepper (Piper nigrum L.)

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1740
Author(s):  
Jing Li ◽  
Rui Fan ◽  
Baoduo Wu ◽  
Xunzhi Ji ◽  
Chaoyun Hao
Keyword(s):  
Gene Family ◽  
Purifying Selection ◽  
Tissue Expression ◽  
Black Pepper ◽  
Piper Nigrum ◽  
Tissue Expression Analysis ◽  
Physiological Processes ◽  
Genome Wide ◽  
Go Enrichment ◽  
A Genome

Black pepper (Piper nigrum L.), is dubbed “the King of Spices”. However, the lack of genic knowledge has limited the understanding of its physiological processes and hindered the development of its molecular breeding. The SBP-box gene family is an important family in plant development and integrates multiple physiological processes. Here, we made a genome-wide identification of the pepper SBP-box gene family to provide evolutionary and functional information about this conserved transcription factor. In total, 34 SBP genes were identified in pepper. All these pepper SBP genes were clustered into eight groups, and one pepper group was not found in Arabidopsis thaliana. Segment duplications played the most important role in the expansion process of pepper SBP genes, and all these duplications were subjected to purifying selection. Half of pepper SBP genes were found miR156 target sites, and 17 miR156s were predicted. The tissue expression analysis revealed the differential expression of pepper SBP genes. Eleven SBP genes were found in four co-expression networks, and the GO enrichment further provides a functional prediction for pepper SBP genes. This study lays a foundation for further studies of pepper and provides a valuable reference for functional mining of pepper SBP genes.

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