Seed storage protein variation in Indian barley using 2-D diagonal gel electrophoresis approach

Barley is the fourth most important cereal, which is used as feed and fodder. It is also used as raw material in the brewing industry and is rich in soluble dietary fiber. Thus, it has economic as well as health benefits. The present study studied seed storage protein characterization of malt, feed, two row, six row, salinity tolerant and susceptible Indian barley genotypes. The polymorphic bands in feed and malt barley were 59% and 54%; in two and six-row barley 35% and 59%; and in salt-tolerant and susceptible barley 38% and 59%. The salt-tolerant lines DL 88 and NB 1were exhibited five interlinked polypeptides, while NDB 1173, NB 3, and RD 2552 exhibited four, two, and one interlinked disulfide linkage, respectively. Whereas, in the case of salt susceptible lines Alfa 93, NB 2, K 508, BH 393 and K 551 a total of six each in Alfa 93 and K 551 barley lines, while in K 508 BH 393 barley lines, two disulfide-linked polypeptides were found. The current study portrayed a simple and economical procedure to characterize the seed protein by gel electrophoresis. Characterization of seed storage proteins by banding pattern analysis using one dimensional SDS-PAGE is the simple molecular technique to evaluate the barley lines with particular traits such as salinity tolerance ability, malt or feed quality, and others. The unique protein bands can also be used as a marker to identify the lines.