scholarly journals Chromosome length genome assembly of the redbanded stink bug, Piezodorus guildinii (Westwood).

2021 ◽  
Author(s):  
Omaththage P. Perera
Keyword(s):  
Genome Assembly ◽  
Population Genetic ◽  
Chromosome Length ◽  
Invasive Pest ◽  
Stink Bug ◽  
High Quality ◽  
Genetic Population ◽  
Redbanded Stink Bug ◽  
The Usa ◽  
Piezodorus Guildinii

Abstract Objective: The redbanded stink bug, Piezodorus guildinii (Hemiptera: Pentatomidae), is native to Caribbean Basin and is currently considered an invasive pest in Florida, Louisiana, Mississippi, and Texas in the southern United States. Although P. guildinii is an economically important invasive pest in the USA, a relatively few studies have been conducted to understand genetic, population genetic structure, and genetic basis of resistance to insecticides. The objective of this work was to obtain a high-quality genome assembly to develop genetic resources to conduct genetic, population genetic, and physiological studies of the RBSB. Results: The genome of RBSB was sequenced with Pacific Biosciences technology followed by two rounds of scaffolding using Chicago libraries and HiC proximity ligation to obtain a high-quality assembly. The genome assembly contained 800 scaffolds larger than 1 kbp and the N50 was 170.84 Mbp. The largest scaffold was 222.22 Mbp and 90% of the genome was included in the 7 scaffolds larger than 1 Mbp. The number of megabase scaffolds also matched the number of chromosomes in this insect. The genome sequence will facilitate the development of resources to conduct studies on genetics, transcriptomics, and physiology of RBSB.

scholarly journals Chromosome-level genome assembly of a benthic associated Syngnathiformes species: the common dragonet, Callionymus lyra

Gigabyte ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Sven Winter ◽  
Stefan Prost ◽  
Jordi de Raad ◽  
Raphael T. F. Coimbra ◽  
Magnus Wolf ◽  
...  
Keyword(s):  
Genome Assembly ◽  
Morphological Differentiation ◽  
Chromosome Length ◽  
Morphological Transformation ◽  
High Quality ◽  
The North ◽  
Repeat Content ◽  
The Common ◽  
Species Specific ◽  
Chromosome Level

Background The common dragonet, Callionymus lyra, is one of three Callionymus species inhabiting the North Sea. All three species show strong sexual dimorphism. The males show strong morphological differentiation, e.g., species-specific colouration and size relations, while the females of different species have few distinguishing characters. Callionymus belongs to the ‘benthic associated clade’ of the order Syngnathiformes. The ‘benthic associated clade’ so far is not represented by genome data and serves as an important outgroup to understand the morphological transformation in ‘long-snouted’ syngnatiformes such as seahorses and pipefishes. Findings Here, we present the chromosome-level genome assembly of C. lyra. We applied Oxford Nanopore Technologies’ long-read sequencing, short-read DNBseq, and proximity-ligation-based scaffolding to generate a high-quality genome assembly. The resulting assembly has a contig N50 of 2.2 Mbp and a scaffold N50 of 26.7 Mbp. The total assembly length is 568.7 Mbp, of which over 538 Mbp were scaffolded into 19 chromosome-length scaffolds. The identification of 94.5% complete BUSCO genes indicates high assembly completeness. Additionally, we sequenced and assembled a multi-tissue transcriptome with a total length of 255.5 Mbp that was used to aid the annotation of the genome assembly. The annotation resulted in 19,849 annotated transcripts and identified a repeat content of 27.7%. Conclusions The chromosome-level assembly of C. lyra provides a high-quality reference genome for future population genomic, phylogenomic, and phylogeographic analyses.

scholarly journals Alkane-priming of Beauveria bassiana strains to improve biocontrol of the redbanded stink bug Piezodorus guildinii and the bronze bug Thaumastocoris peregrinus

2021 ◽  
Author(s):  
Lucia Sessa ◽  
Nicolas Pedrini ◽  
Nora Altier ◽  
Eduardo Abreo
Keyword(s):  
Stress Tolerance ◽  
Beauveria Bassiana ◽  
Stink Bug ◽  
High Susceptibility ◽  
Bronze Bug ◽  
Redbanded Stink Bug ◽  
Fungal Entomopathogens ◽  
Cytochrome P450 Genes ◽  
Beauveria Bassiana Isolates ◽  
Piezodorus Guildinii

Insect Epicuticle hydrocarbons (CHC) are known to be important determinants in the susceptibility degree of insects to fungal entomopathogens. Five Beauveria bassiana isolates were phenotypically analyzed regarding their response to CHC nutrition and their pathogenicity and virulence towards high fungal-susceptible Thaumastocoris peregrinus and low fungal-susceptible Piezodorus guildinii, which are important hemipteran pests in eucalyptus and soybean plantations, respectively. Two of these isolates, resulting the most (ILBB308) and the least (ILBB299) virulent to P. guildinii, were also evaluated at gene expression level after growth on n-pentadecane. B. bassiana most virulent isolate ILBB308 showed the lowest growth on most evaluated CHC media. However, this isolate distinctively induced most of the analyzed genes involved in CHC assimilation, cuticle degradation and stress tolerance. Virulence towards low susceptibility P. guildinii was enhanced in both hypervirulent ILB308 and hypovirulent ILBB299 isolates after growth on n-pentadecane as the sole carbon source, whereas virulence enhancement towards high susceptibility T. peregrinus was not observed in alkane-grown fungi. Virulence enhancement towards P. guildinii could be mostly explained by a priming effect produced by CHC on the induction of some genes related to hydrocarbon assimilation in ILB 205 and ILB 308, such as hydrophobin (Bbhyd2) and cytochrome P450 genes (BbCyp52g11 and BbCyp52x1), and partially by the induction of genes related to cuticle degradation (Bbchit and Bbcdep1) and stress tolerance (Bbsod1) observed only in ILB308.

Characterization of antibiosis to the redbanded stink bug Piezodorus guildinii (Hemiptera: Pentatomidae) in soybean entries

2013 ◽  
Vol 86 (4) ◽  
pp. 649-657 ◽  
Author(s):  
José P. G. F. Silva ◽  
Edson L. L. Baldin ◽  
Efrain S. Souza ◽  
Vinícius F. Canassa ◽  
André L. Lourenção
Keyword(s):  
Stink Bug ◽  
Redbanded Stink Bug ◽  
Piezodorus Guildinii

scholarly journals A Male-produced Sex Pheromone from the Neotropical Redbanded Stink Bug, Piezodorus guildinii (W.)

2007 ◽  
Vol 33 (6) ◽  
pp. 1235-1248 ◽  
Author(s):  
Miguel Borges ◽  
Jocelyn G. Millar ◽  
R. A. Laumann ◽  
Maria C. B. Moraes
Keyword(s):  
Sex Pheromone ◽  
Stink Bug ◽  
Redbanded Stink Bug ◽  
Piezodorus Guildinii

scholarly journals Chromosome-level genome assembly of a benthic associated Syngnathiformes species: the common dragonet, Callionymus lyra

2020 ◽  
Author(s):  
Sven Winter ◽  
Stefan Prost ◽  
Jordi de Raad ◽  
Raphael T. F. Coimbra ◽  
Magnus Wolf ◽  
...  
Keyword(s):  
Genome Assembly ◽  
Morphological Differentiation ◽  
Chromosome Length ◽  
Morphological Transformation ◽  
High Quality ◽  
The North ◽  
Repeat Content ◽  
The Common ◽  
Species Specific ◽  
Chromosome Level

AbstractBackgroundThe common dragonet, Callionymus lyra, is one of three Callionymus species inhabiting the North Sea. All three species show strong sexual dimorphism. The males show strong morphological differentiation, e.g., species-specific colouration and size relations, while the females of different species have few distinguishing characters. Callionymus belongs to the ‘benthic associated clade’ of the order Syngnathiformes. The ‘benthic associated clade’ so far is not represented by genome data and serves as an important outgroup to understand the morphological transformation in ‘long-snouted’ syngnatiforms such as seahorses and pipefishes.FindingsHere, we present the chromosome-level genome assembly of C. lyra. We applied Oxford Nanopore Technologies’ long-read sequencing, short-read DNBseq, and proximity-ligation-based scaffolding to generate a high-quality genome assembly. The resulting assembly has a contig N50 of 2.2 Mbp, a scaffold N50 of 26.7 Mbp. The total assembly length is 568.7 Mbp, of which over 538 Mbp were scaffolded into 19 chromosome-length scaffolds. The identification of 94.5% of complete BUSCO genes indicates high assembly completeness. Additionally, we sequenced and assembled a multi-tissue transcriptome with a total length of 255.5 Mbp that was used to aid the annotation of the genome assembly. The annotation resulted in 19,849 annotated transcripts and identified a repeat content of 27.66%.ConclusionsThe chromosome-level assembly of C. lyra provides a high-quality reference genome for future population genomic, phylogenomic, and phylogeographic analyses.

Sign in / Sign up

Export Citation Format

Share Document