In-Vivo Functional and Structural Retinal Imaging Using Multiwavelength Photoacoustic Remote Sensing Microscopy and Optical Coherence Tomography

Abstract A multiwavelength photoacoustic remote sensing (PARS) microscope is combined with swept-source optical coherence tomography and applied to in-vivo, non-contact retinal imaging. Building on the proven strength of multiwavelength PARS imaging, the system is applied for estimating retinal oxygen saturation in the rat eye. The capability of the technology is demonstrated by imaging both microanatomy and the microvasculature of the retina in-vivo. To our knowledge, this is the first time a non-contact photoacoustic imaging technique is employed for in-vivo oxygen saturation measurement in the retina.

Download Full-text

Dual-modality photoacoustic remote sensing (PARS) microscopy and swept-source optical coherence tomography (SS-OCT) for in-vivo imaging

Photons Plus Ultrasound: Imaging and Sensing 2021 ◽

10.1117/12.2578036 ◽

2021 ◽

Author(s):

Zohreh Hosseinaee ◽

Nima Abbasi ◽

Alex Tummon Simmons ◽

Layla Khalil ◽

Kevan L. Bell ◽

...

Keyword(s):

Remote Sensing ◽

Optical Coherence Tomography ◽

In Vivo Imaging ◽

Optical Coherence ◽

Swept Source ◽

Dual Modality

Download Full-text

Non-contact, in-vivo, functional, and structural ophthalmic imaging using multimodal photoacoustic remote sensing (PARS) microscopy and swept source optical coherence tomography (SS-OCT)

10.21203/rs.3.rs-289633/v1 ◽

2021 ◽

Author(s):

Zohreh Hosseinaee ◽

Nima Abbasi ◽

Layla Khalili ◽

Lyazzat Mukhangaliyeva ◽

Nicholas Pellegrino ◽

...

Keyword(s):

Remote Sensing ◽

Optical Coherence Tomography ◽

Pigment Epithelium ◽

Retinal Pigment ◽

Ocular Tissue ◽

Optical Coherence ◽

Structural Imaging ◽

Ocular Diseases ◽

Swept Source

Abstract Early diagnosis of ocular diseases improves the understanding of pathophysiology and aids in accurate monitoring and effective treatment. Advanced, multimodal ocular imaging platforms play a crucial role in visualization of ocular components and provide clinicians with a valuable tool for evaluating various eye diseases. Here, for the first time we present a non-contact, multiwavelength photoacoustic remote sensing (PARS) microscopy and swept-source optical coherence tomography (SS-OCT) for in-vivo functional and structural imaging of the eye. The system provides complementary imaging contrasts of optical absorption and optical scattering, and is used for non-contact, in-vivo imaging of murine eye. Results of vasculature and structural imaging as well as melanin content in the retinal pigment epithelium layer are presented. Multiwavelength PARS microscopy using Stimulated Raman Scattering is applied to enable in-vivo, non-contact oxygen saturation estimation in the ocular tissue. The reported work may be a major step towards clinical translation of ophthalmic technologies and has the potential to advance the diagnosis and treatment of ocular diseases.

Download Full-text

Quantitative Swept-Source Optical Coherence Tomography of Early Enamel Erosion in vivo

Caries Research ◽

10.1159/000477098 ◽

2017 ◽

Vol 51 (4) ◽

pp. 410-418 ◽

Cited By ~ 5

Author(s):

Rupert S. Austin ◽

Maisalamah Haji Taha ◽

Frederic Festy ◽

Richard Cook ◽

Manoharan Andiappan ◽

...

Keyword(s):

Optical Coherence Tomography ◽

Healthy Volunteers ◽

Orange Juice ◽

Signal Intensity ◽

Surface States ◽

Enamel Surface ◽

Optical Coherence ◽

Swept Source ◽

Water Rinsing

Swept-source optical coherence tomography (SS-OCT) shows potential for the in vivo quantitative evaluation of micro-structural enamel surface phenomena occurring during early erosive demineralization. This randomized controlled single-blind cross-over clinical study aimed to evaluate the use of SS-OCT for detecting optical changes in the enamel of 30 healthy volunteers subjected to orange juice rinsing (erosive challenge) in comparison to mineral water rinsing (control), according to wiped and non-wiped enamel surface states. Participants were randomly allocated to 60 min of orange juice rinsing (pH 3.8) followed by 60 min of water rinsing (pH 6.7) and vice versa, with a 2-week wash-out period. In addition, the labial surfaces of the right or left maxillary incisors were wiped prior to SS-OCT imaging. An automated ImageJ algorithm was designed to analyse the back-scattered OCT signal intensity (D) after orange juice rinsing compared to after water rinsing. D was quantified as the OCT signal scattering from the 33 µm sub-surface enamel, normalised by the total OCT signal intensity entering the enamel. The back-scattered OCT signal intensity increased by 3.1% (95% CI 1.1-5.1%) in the wiped incisors and by 3.5% (95% CI 1.5-5.5%) in the unwiped incisors (p < 0.0001). Wiping reduced the back-scattered OCT signal intensity by 1.7% (95% CI -3.2 to -0.3%; p = 0.02) in comparison to the unwiped enamel surfaces for both rinsing solutions (p = 0.2). SS-OCT detected OCT signal changes in the superficial sub-surface enamel of maxillary central incisor teeth of healthy volunteers after orange juice rinsing.

Download Full-text