Secretory Heat Shock Protein - gp96-Ig Chaperoned Her-2/new Vaccines

1999 ◽  
Author(s):  
Eckhard Podack
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Heat Shock Protein Gp96 ◽  
Her 2 ◽  
New Vaccines

scholarly journals Cross-priming of minor histocompatibility antigen-specific cytotoxic T cells upon immunization with the heat shock protein gp96.

1995 ◽  
Vol 182 (3) ◽  
pp. 885-889 ◽  
Author(s):  
D Arnold ◽  
S Faath ◽  
H Rammensee ◽  
H Schild
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Heat Shock Protein ◽  
Tumor Cells ◽  
Mhc Class I ◽  
Donor Cell ◽  
Class I ◽  
Heat Shock Protein Gp96 ◽  
Shock Proteins

Vaccination of mice with heat shock proteins isolated from tumor cells induces immunity to subsequent challenge with those tumor cells the heat shock protein was isolated from but not with other tumor cells (Udono, H., and P.K. Srivastava. 1994. J. Immunol. 152:5398-5403). The specificity of this immune response is caused by tumor-derived peptides bound to the heat shock proteins (Udono., H., and P.K. Srivastava. 1993. J. Exp. Med. 178:1391-1396). Our experiments show that a single immunization with the heat shock protein gp96 isolated from beta-galactosidase (beta-gal) expressing P815 cells (of DBA/2 origin) induces cytotoxic T lymphocytes (CTLs) specific for beta-gal, in addition to minor H antigens expressed by these cells. CTLs can be induced in mice that are major histocompatibility complex (MHC) identical to the gp96 donor cells (H-2d) as well as in mice with a different MHC (H-2b). Thus gp96 is able to induce "cross priming" (Matzinger, P., and M.J. Bevan. 1977. Cell. Immunol. 33:92-100), indicating that gp96-associated peptides are not limited to the MHC class I ligands of the gp96 donor cell. Our data confirm the notion that samples of all cellular antigens presentable by MHC class I molecules are represented by peptides associated with gp96 molecules of that cell, even if the fitting MHC molecule is not expressed. In addition, we extend previous reports on the in vivo immunogenicity of peptides associated gp96 molecules to two new groups of antigens, minor H antigens, and proteins expressed in the cytosol.

scholarly journals The Endoplasmic Reticulum-resident Heat Shock Protein Gp96 Activates Dendritic Cells via the Toll-like Receptor 2/4 Pathway

2002 ◽  
Vol 277 (23) ◽  
pp. 20847-20853 ◽  
Author(s):  
Ramunas M. Vabulas ◽  
Sibylla Braedel ◽  
Norbert Hilf ◽  
Harpreet Singh-Jasuja ◽  
Sylvia Herter ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Dendritic Cells ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Toll Like Receptor ◽  
Heat Shock Protein Gp96 ◽  
Toll Like Receptor 2

scholarly journals 86 – Heat Shock Protein Gp96 is Essential for Maintaining Intestinal Epithelial Architecture by Supporting Its Self-Renewal Capacity

2019 ◽  
Vol 156 (6) ◽  
pp. S-19
Author(s):  
Janine Haefliger ◽  
Marlene Schwarzfischer ◽  
Marnix Van de Sande ◽  
Roberto Manzini ◽  
Claudia Stanzel ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Intestinal Epithelial ◽  
Self Renewal ◽  
Heat Shock Protein Gp96

scholarly journals Cutting Edge: The Heat Shock Protein gp96 Activates Inflammasome-Signaling Platforms in APCs

2018 ◽  
Vol 201 (8) ◽  
pp. 2209-2214 ◽  
Author(s):  
Yifei Wang ◽  
Abigail L. Sedlacek ◽  
Sudesh Pawaria ◽  
Haiyan Xu ◽  
Melanie J. Scott ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Cutting Edge ◽  
Heat Shock Protein Gp96

810 - Heat Shock Protein GP96 is Essential for Self-Renewal of the Intestinal Epithelial Barrier

2018 ◽  
Vol 154 (6) ◽  
pp. S-166
Author(s):  
Marlene Schwarzfischer ◽  
Yasser Morsy ◽  
Claudia Stanzel ◽  
Kirstin Atrott ◽  
Silvia Lang ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Intestinal Epithelial Barrier ◽  
Self Renewal ◽  
Heat Shock Protein Gp96

scholarly journals Cutting Edge: Heat Shock Protein gp96 Induces Maturation and Migration of CD11c+ Cells In Vivo

2000 ◽  
Vol 165 (11) ◽  
pp. 6029-6035 ◽  
Author(s):  
Robert J. Binder ◽  
Kirstin M. Anderson ◽  
Sreyashi Basu ◽  
Pramod K. Srivastava
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Cutting Edge ◽  
Heat Shock Protein Gp96 ◽  
And Migration

The Significance of Heat-Shock Protein GP96 and its Receptors' CD91 and Toll-Like Receptor 4 Expression at the Maternal Foetal Interface

2013 ◽  
Vol 70 (1) ◽  
pp. 10-23 ◽  
Author(s):  
Tamara Gulic ◽  
Gordana Laskarin ◽  
Arnela Redzovic ◽  
Senija Eminović ◽  
Herman Haller ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Toll Like Receptor ◽  
Toll Like Receptor 4 ◽  
Heat Shock Protein Gp96

scholarly journals Biophysical Analysis of the Endoplasmic Reticulum-Resident Chaperone/Heat Shock Protein gp96/GRP94 and Its Complex with Peptide Antigen†

Biochemistry ◽  
2001 ◽  
Vol 40 (5) ◽  
pp. 1483-1495 ◽  
Author(s):  
Nora A. Linderoth ◽  
Martha N. Simon ◽  
Natalia A. Rodionova ◽  
Martine Cadene ◽  
William R. Laws ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Heat Shock ◽  
Heat Shock Protein ◽  
Peptide Antigen ◽  
Biophysical Analysis ◽  
Heat Shock Protein Gp96

Identification and expression distribution of esophageal carcinoma autoantigens.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e16520-e16520
Author(s):  
Fuchun Si
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Esophageal Carcinoma ◽  
Normal Tissue ◽  
Proteome Analysis ◽  
Tumor Rejection ◽  
Cancer Tissue ◽  
Carcinoma Tissue ◽  
Heat Shock Protein Gp96 ◽  
Tumor Rejection Antigen

e16520 Background: To identify the autoantigen protein molecules with autoserum in the tissues from the esophageal carcinoma (EC) patients, analyze autoantigen expression distribution in EC tissues, so as to provide basis for the molecular pathogenesis and clinical medication of EC. Methods: 69 cases of EC patients tissues and serum and 81 cases of healthy people serum were collected, serological proteome analysis (SERPA) was modified with sequential extraction of subcellular protein fractions to identify esophageal oncopathgensis stages autoantigen with autoserum in the tissues from the EC patients. Another 93 cases of EC patients tissue were collected, immunohistochemical and western blot method were used to detect expression distribution of EC autoantigens in esophageal carcinoma tissue, para-carcinoma tissue and normal tissue. Results: Autoantigens CK13, CK16, CaD, ACTG2, tumor rejection antigen (gp96) 1 variant, heat shock protein gp96 precursor were identified, among wihich, CK16, CaD, ACTG2, tumor rejection antigen (gp96)1 variant, heat shock protein gp96 precursor were firstly reported as EC autoantigens. Expression of autoantigens CK16, CaD, ACTG2 were increased in EC carcinoma tissue than para-carcinoma tissue and normal tissue, while CK13 were decreased. Positive expression level of CK16 in normal tissue, para-carcinoma tissue and cancer tissue of EC patients was 0.0076±0.0033, 0.0158±0.0065, 0.0356±0.0165 respectively, CaD was 0.0085±0.0048, 0.0107±0.0056, 0.0177±0.0103 respectively, ACTG2 was 0.0091±0.0039, 0.0136±0.0043, 0.0214±0.0110 respectively, and CK13 was 0.2053±0.0311, 0.1633±0.0280, 0.0412±0.0239 respectively. Conclusions: 6 EC autoantigens were identified, and 5 were first reported. Autoantigens CK13, CK16, CaD and ACTG2 were expressed in EC patients carcinoma tissue, which can be the potential biomarkers of esophageal carcinoma. This study provides new basis for the EC molecular mechanism and development of molecular drugs.

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