Determining Optimal Microwave Antigen Retrieval Conditions for Microtubule-Associated Protein 2 Immunohistochemistry in the Guinea Pig Brain

2002 ◽  
Author(s):  
Christina M. Pleva ◽  
Tracey A. Hamilton ◽  
John P. Petrali ◽  
Robert K. Kan
Keyword(s):  
Guinea Pig ◽  
Antigen Retrieval ◽  
Pig Brain ◽  
Guinea Pig Brain

Immunolocalization of MAP-2 in Routinely Formalin-Fixed, Paraffin-Embedded Guinea Pig Brain Sections Using Microwave Irradiation: A Comparison of Different Combinations of Antibody Clones and Antigen Retrieval Buffer Solutions

2005 ◽  
Vol 11 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Robert K. Kan ◽  
Christina M. Pleva ◽  
Tracey A. Hamilton ◽  
John P. Petrali
Keyword(s):  
Citric Acid ◽  
Guinea Pig ◽  
Nerve Agent ◽  
Antigen Retrieval ◽  
Microwave Pretreatment ◽  
Pig Brain ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Guinea Pig Brain ◽  
Formalin Fixed

The present study was designed to evaluate the efficacy of different microwave pretreatment methods to retrieve microtubule-associated protein 2 (MAP-2) immunoreactivity in formalin-fixed, paraffin-embedded guinea pig brain sections. Brain sections, microwave pretreated in boiling sodium citrate, citric acid, Tris hydrochloride, and EDTA buffers of pH 4, 6, and 8, were labeled with four different clones of MAP-2 monoclonal antibodies. No MAP-2 immunoreactivity was observed in control sections processed without microwave pretreatment. Optimal MAP-2 immunoreactivity was observed only when MAP-2 antibody clone AP18 was used in conjunction with citric acid buffer of pH 6.0. Using this combination, brain sections from nerve agent soman-exposed guinea pigs were found to exhibit marked reduction in MAP-2 immunostaining in the hippocampus. These observations suggest that the clone of the antibody in addition to the type and pH of antigen retrieval (AR) solution are important variables to be considered for establishing an optimal AR technique. When studying counterpart antigens of species other than that to which the antibodies were originally raised, different antibody clones must be tested in combination with different microwave-assisted AR (MAR) methods. This MAR method makes it possible to conduct retrospective studies on archival guinea pig brain paraffin blocks to evaluate changes in neuronal MAP-2 expression as a consequence of chemical warfare nerve agent toxicity.

High-frequency oscillations and seizure-like discharges in the entorhinal cortex of the in vitro isolated guinea pig brain

2017 ◽  
Vol 130 ◽  
pp. 21-26 ◽  
Author(s):  
Laura Uva ◽  
Davide Boido ◽  
Massimo Avoli ◽  
Marco de Curtis ◽  
Maxime Lévesque
Keyword(s):  
Guinea Pig ◽  
Entorhinal Cortex ◽  
High Frequency ◽  
High Frequency Oscillations ◽  
Pig Brain ◽  
Guinea Pig Brain

Drug induced changes in free, labile and stable acetylcholine of guinea-pig brain

1969 ◽  
Vol 18 (6) ◽  
pp. 1315-1324 ◽  
Author(s):  
L. Beani ◽  
C. Bianchi ◽  
P. Megazzini ◽  
L. Ballotti ◽  
G. Bernardi
Keyword(s):  
Guinea Pig ◽  
Drug Induced ◽  
Pig Brain ◽  
Induced Changes ◽  
Guinea Pig Brain

Distribution and characterization of 5-HT1D binding sites in the guinea-pig brain: an autoradiographic analysis

1990 ◽  
Vol 183 (3) ◽  
pp. 1109-1110
Author(s):  
H. Sijbesma ◽  
J.C.H.M. Cornelissen ◽  
E.R. de Kloet ◽  
J. Schipper
Keyword(s):  
Guinea Pig ◽  
Binding Sites ◽  
Pig Brain ◽  
Autoradiographic Analysis ◽  
Guinea Pig Brain
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