Optimization of Glial Fibrillary Acidic Protein Immunoreactivity in Formalin-fixed, Paraffin-Embedded Guinea Pig Brain Sections

2003 ◽  
Author(s):  
Christina P. Tompkins ◽  
Tracey A. Hamilton ◽  
John P. Petrall ◽  
Robert K. Kan
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Guinea Pig ◽  
Acidic Protein ◽  
Pig Brain ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Protein Immunoreactivity ◽  
Guinea Pig Brain ◽  
Formalin Fixed

scholarly journals Microwave-Assisted MAP-2 Immunoreactivity in Formalin-Fixed, Paraffin-Embedded Guinea Pig Brain

2002 ◽  
Vol 8 (S02) ◽  
pp. 736-737
Author(s):  
Robert K. Kan ◽  
Christina M. Pleva ◽  
Tracey A. Hamilton ◽  
John P. Petrali
Keyword(s):  
Guinea Pig ◽  
Microwave Assisted ◽  
Pig Brain ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Guinea Pig Brain ◽  
Formalin Fixed

Immunolocalization of MAP-2 in Routinely Formalin-Fixed, Paraffin-Embedded Guinea Pig Brain Sections Using Microwave Irradiation: A Comparison of Different Combinations of Antibody Clones and Antigen Retrieval Buffer Solutions

2005 ◽  
Vol 11 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Robert K. Kan ◽  
Christina M. Pleva ◽  
Tracey A. Hamilton ◽  
John P. Petrali
Keyword(s):  
Citric Acid ◽  
Guinea Pig ◽  
Nerve Agent ◽  
Antigen Retrieval ◽  
Microwave Pretreatment ◽  
Pig Brain ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Guinea Pig Brain ◽  
Formalin Fixed

The present study was designed to evaluate the efficacy of different microwave pretreatment methods to retrieve microtubule-associated protein 2 (MAP-2) immunoreactivity in formalin-fixed, paraffin-embedded guinea pig brain sections. Brain sections, microwave pretreated in boiling sodium citrate, citric acid, Tris hydrochloride, and EDTA buffers of pH 4, 6, and 8, were labeled with four different clones of MAP-2 monoclonal antibodies. No MAP-2 immunoreactivity was observed in control sections processed without microwave pretreatment. Optimal MAP-2 immunoreactivity was observed only when MAP-2 antibody clone AP18 was used in conjunction with citric acid buffer of pH 6.0. Using this combination, brain sections from nerve agent soman-exposed guinea pigs were found to exhibit marked reduction in MAP-2 immunostaining in the hippocampus. These observations suggest that the clone of the antibody in addition to the type and pH of antigen retrieval (AR) solution are important variables to be considered for establishing an optimal AR technique. When studying counterpart antigens of species other than that to which the antibodies were originally raised, different antibody clones must be tested in combination with different microwave-assisted AR (MAR) methods. This MAR method makes it possible to conduct retrospective studies on archival guinea pig brain paraffin blocks to evaluate changes in neuronal MAP-2 expression as a consequence of chemical warfare nerve agent toxicity.

Determining Optimal Microwave Antigen Retrieval Conditions for Microtubule-Associated Protein 2 Immunohistochemistry in the Guinea Pig Brain

2002 ◽  
Author(s):  
Christina M. Pleva ◽  
Tracey A. Hamilton ◽  
John P. Petrali ◽  
Robert K. Kan
Keyword(s):  
Guinea Pig ◽  
Antigen Retrieval ◽  
Pig Brain ◽  
Guinea Pig Brain

Proteome Analysis of Formalin-Fixed Paraffin-Embedded Tissues from a Primary Gastric Melanoma and its Meningeal Metastasis: A Case Report

2014 ◽  
Vol 14 (3) ◽  
pp. 382-387 ◽  
Author(s):  
Juliana Fischer ◽  
Nathalie Canedo ◽  
Katia Goncalves ◽  
Leila Chimelli ◽  
Monique Franca ◽  
...  
Keyword(s):  
Case Report ◽  
Proteome Analysis ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed
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